• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.4
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• pp.819-824
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• 2015

SDN (Software Defined Network) technology, hardware and network control functions should be separated independently of existing switches or routers and data transfer functions and are also refers to a separate network architecture that can develop and run. When planning a network and designed to reflect the network control function by applying the SDN may reflect the resources efficiently. In this study, one people suggest Applications SDN, which offer an absolutely necessary part of a coded program that combines the benefits of the existing IOS (Combined Benefits) applying the model to complete the Controller part through the MPLS IOS. The OpenFlow Controller is a command to the router LER(Label Edge Router), and transmits the packet in accordance with the command to the destination according to the EXP bit. To configure TCP / IP to the IOS through the optimized coding. Using the OpenFlow protocol controller transmits an encapsulation header as defined in the MPLS packet.

• Journal of KIISE:Information Networking
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• v.34 no.1
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• pp.48-61
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• 2007

WiBro (Wireless Broadband) service, developed in Korea, can provide the host mobility while its users hang around within the subnet. Next-generation Internet protocols, IPv6 and Mobile IPv6 (MIPv6), provide a plenty of addresses to the nodes and enable the handover between different subnets. However, MIPv6 is not enough to support a real time service such as VoIP (Voice over IP) due to the long latency, and it is necessary to develop an enhanced handover mechanism which is optimized to the WiBro networks. In this paper, we suggest an improved fast handover mechanism while the mobile node moves around WiBro networks. The proposal is based on Fast Mobile IPv6 (FMIPv6) which is the representative protocol for fast handover, and reduces the handover latency by the close interaction between the link layer (WiBro MAC) and IP layer (FMIPv6). Finally, we analyze the performance of proposed mechanism through the mathematical analysis.

• Journal of Embryo Transfer
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• v.28 no.2
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• pp.87-93
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• 2013

The efficiency of artificial insemination (AI) for horses remains unsatisfactory. It is mainly because each process of AI causes a detrimental effect on semen quality. To sustain quality of semen properly, several factors including libido of stallions and sperm damage during sperm processing and preservation should be considered. Stallions with decent libido produce a high ratio of sperm to seminal plasma in their ejaculates, which is the ideal semen composition for maintaining sperm quality. Thus, to maximize the fertility rate upon AI, stallions should be appropriately managed to enhance their libido. Seminal plasma should have a positive effect on horse fertility in the case of natural breeding, whereas the effects of seminal plasma on both sperm viability and quality in the context of AI remain controversial. Centrifugation of semen is performed during semen processing to remove seminal plasma and to isolate fine quality sperm from semen. However, the centrifugation process can also result in sperm loss and damage. To solve this problem, several different centrifugation techniques such as Cushion Fluid along with dual and single Androcoll-E$^{TM}$ were developed to minimize loss of sperm and to damage at the bottom of the pellet. Most recently, a new technique without centrifugation was developed with the purpose of separating sperm from semen. AI techniques have been advanced to deliver sperm to optimal region of female reproductive tract at perfect timing. Recombinant equine luteinizing hormone (reLH) and low dose insemination techniques have been developed to maximize both fertility rate and the efficiency of AI. Horse breeders should consider that the entire AI procedure should be optimized for each stallion due to variation in individual horses for a uniformed AI protocol.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.8
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• pp.1171-1177
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• 2015

The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of myogenic precursor cells in postnatal muscle. Muscle satellite cells are a useful model to investigate the mechanisms of muscle growth and development. Although the isolation and culture protocols of muscle satellite cells in some species (e.g. mouse) have been established successfully, the culture system for porcine muscle satellite cells is very limited. In this study, we optimized the isolation procedure of porcine muscle satellite cells and elaborated the isolation and culture process in detail. Furthermore, we characterized the porcine muscle satellite cells using the immunofluorecence. Our study provides a reference for the isolation of porcine muscle satellite cells and will be useful for studying the molecular mechanisms in these cells.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.3
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• pp.223-231
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• 2020

Karyotype analysis is a major work in the process of triploid abalone production for the purpose of productivity and quality improvement. However, the metaphase spreads for karyotype analysis have been prepared just from the larvae at trochophore stage, which has restricted the spectrum of sample correction inhibiting more efficient analysis. Here, we investigated the feasibility of preparing metaphase spreads from the larvae at veliger stage that is the next developmental stage of trochophore. For this, diploid and triploid larvae at trochophore and veliger stages from Pacific abalone (Haliotis discus hannai) were subjected to metaphase spread preparation and its efficiencies were measured and compared each other. As the results, although the efficiencies of metaphase spread preparation were significantly lower in the larvae at veliger stage compared to the ones at trochophore stage regardless of ploidy status, we found that the preparation of metaphase spreads, which showed the clear chromosomal images containing the normal number of chromosomes, was possible from the veliger stage larvae. On the other hands, all larvae used in this study regardless of developmental stage and ploidy did not show colchicine sensitivity. Moreover, no significant difference was observed in cell cycle distribution of the cells comprising larvae between two developmental stages regardless of ploidy status. These suggested that the details of protocol to prepare metaphase spreads from abalone larvae should be optimized depending on its developmental stages. Taken together, we demonstrated the feasibility of preparing metaphase spreads from H. discus hannai veliger stage larvae for karyotype analysis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.10
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• pp.2733-2740
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• 2009

An advanced and proactive response mechanism against diverse attacks on All-IP network should be proposed for enhance its security and reliability on open network. There are two main research works related to this study. First one is the SPIE system with hash function on Bloom filter and second one is the Sinkhole routing mechanism using BGP protocol for verifying its transmission path. In this study, we proposed an advanced IP Tracing mechanism based on Bloom filter and Sinkhole routing mechanism. Proposed mechanism has a Manager module for controlling the regional router with using packet monitoring and filtering mechanism to trace and find the attack packet's real transmission path. Additionally, proposed mechanism provides advanced packet aggregation and monitoring/control module based on existing Sinkhole routing method. Therefore, we can provide an optimized one in All-IP network by combining the strength on existing two mechanisms. And the Tracing performance also can be enhanced compared with previously suggested mechanism.

• Journal of Biomedical Engineering Research
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• v.27 no.6
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• pp.392-401
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• 2006

Image Guided Surgery (IGS) system which has variously tried in medical engineering fields is able to give a surgeon objective information of operation process like decision making and surgical planning. This information is displayed through 3D images which are acquired from image modalities like CT and MRI for pre-operation. The technique of image registration is necessary to construct IGS system. Image registration means that 3D model and the object operated by a surgeon are matched on the common frame. Major techniques of registration in IGS system have been used by recognizing fiducial markers placed on the object. However, this method has been criticized due to additional trauma, its invasive protocol inserting fiducial markers in patient's bone and generating noise data when 2D slice images are acquired by image modality because many markers are made of metal. Therefore, this paper developed shape-based registration technique to improve the limitation of fiducial marker based IGS system. Iterative Closest Points (ICP) algorithm was used to match corresponding points and quaternion based rotation and translation transformation using closed form solution applied to find the optimized cost function of transformation. we assumed that this algorithm were used in Total Knee replacement (TKR) operation. Accordingly, we have developed region-based 3D registration technique based on anatomical landmarks and this registration algorithm was evaluated in a femur model. It was found that region-based algorithm can improve the accuracy in 3D registration.

• Korean Journal of Medicinal Crop Science
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• v.15 no.5
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• pp.346-351
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• 2007

An efficient somatic embryogenesis and plant regeneration protocol was developed for Schisandra chinensis Baill, using embryogenic cell suspensions and optimized media conditions. Friable embryogenic callus was induced from cotyledonary leaf and hypocotyl explants of 7 days old seedlings on MS agar medium supplemented with 1.0 to $4.0\;mg\;l^{-1}$ of 2,4-dichlorophenoxyacetic acid (2,4-D). Fast growing and well dispersed embryogenic cell suspensions were developed within two months when embryogenic calli were transferred to MS liquid medium containing $1.0\;mg\;l^{-1}\;2,4-D$. One third strength of MS medium was the best for both overall growth and development of somatic embryos in liquid culture. Over 3400 viable somatic embryos were produced from each 150 ml flask with an initial cell density of 30 mg in 30 ml medium. Germinated somatic embryos developed in liquid medium converted into plantlets after transferred to half-strength MS semi-solid medium. Approximately 90% of the converted plantlets were successfully transplanted to soil and grew into fertile plants.

• The Journal of the Korea Contents Association
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• v.12 no.12
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• pp.46-53
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• 2012

The traffic in research network for the new structure of the network and new service research has the various properties. From the perspective of a specific traffic point of view, transmission of traffic with different requirements using a single routing protocol is an obstacle to satisfy requirements. In this study, we classify the properties of the traffic into two types. We propose the way that we can get the overall optimization effect, the experiment proves it by providing independent multiple forwarding path by applying optimized algorithm by types. In order to distinguish each type of traffic we use the ports on the switch and in order to implement independent path we apply OpenFlow system. In other words, we present the measure that can be implemented to improve the satisfaction of the traffic by making multiple paths by OpenFlow controller according to the type of traffic and by enforcing in Forwarder.

• ALGAE
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• v.31 no.4
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• pp.373-378
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• 2016

Cochlodinium polykrikoides is a red-tide forming dinoflagellate that causes significant worldwide impacts on aquaculture industries and the marine ecosystem. There have been extensive studies on managing and preventing C. polykrikoides blooms, but it has been difficult to identify an effective method to control the bloom development. There is also limited genome information on the molecular mechanisms involved in its various ecophysiology and metabolism processes. Thus, comprehensive genome information is required to better understand harmful algal blooms caused by C. polykrikoides. We estimated the C. polykrikoides genome size using flow cytometry, with detection of the fluorescence of DNA stained with propidium iodide (PI). The nuclear genome size of C. polykrikoides was 100.97 Gb, as calculated by comparing its mean fluorescence intensity (MFI) to the MFI of Mus musculus, which is 2.8 Gb. The exceptionally large genome size of C. polykrikoides might indicate its complex physiological and metabolic characteristics. Our optimized protocol for estimating the nuclear genome size of a dinoflagellate using flow cytometry with PI can be applied in studies of other marine organisms.