• Korean Journal of Food Science and Technology
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• v.30 no.2
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• pp.397-406
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• 1998

Hen egg white lysozyme (HEWL) is very valuable as a natural preservative in food processing due to its selective bactericidal activity. HEWL which traditionally isolated by crystallization or freeze drying was simply separated from 13 different hen egg white (HEW) proteins by a single-step ultrafiltration. Freeze dried HEW (0.25%, w/v) dissolved in a citrate-phosphate buffer (pH 4.6) was ultrafiltered with a PM30 membrane under various operating conditions, by changing concentration, temperature, transmembrane pressure $({\triangle}P_T)$, and stirring speed. Optimum separation conditions were decided when maximal flux was obtained. Under the optimum separation conditions, the effect of membrane material and fouling on flux as time passed as well as lysozyme concentration, protein concentration, specific activity (SA) in the permeate were measured. Best separation conditions of HEWL with PM30 membrane were sample concentration 0.25%, temperature $35^{\circ}C$, ${\Delta}P_T\;30\;psi$, and stirring speed 300 rpm. During the first 12 min, the flux of YM30 was higher, but at the steady-state it was lower than that of PM30. The SA of the PM30 permeate was over 2 times higher in spite of the lysozyme and protein concentration being lower than that of YM30 permeate. The flux of 5 times used PM30 decreased 30% compared to a new PM30, but both had the same tendency in flux decrease when time passed. Both of them reached a steady-state after 35 min and remained at 70% of the initial flux. In the PM30 permeate, the lysozyme concentration and SA were 110 units/mL and 2,821 units/mg protein, respectively. Therefore, PM30 membrane separation was very effective for separation of antimicrobial lysozyme.

• Proceedings of the Korea Inteligent Information System Society Conference
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• 1999.03a
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• pp.175-186
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• 1999

Detecting the features of significant patterns from their own historical data is so much crucial to good performance specially in time-series forecasting. Recently, a new data filtering method (or multi-scale decomposition) such as wavelet analysis is considered more useful for handling the time-series that contain strong quasi-cyclical components than other methods. The reason is that wavelet analysis theoretically makes much better local information according to different time intervals from the filtered data. Wavelets can process information effectively at different scales. This implies inherent support for multiresolution analysis, which correlates with time series that exhibit self-similar behavior across different time scales. The specific local properties of wavelets can for example be particularly useful to describe signals with sharp spiky, discontinuous or fractal structure in financial markets based on chaos theory and also allows the removal of noise-dependent high frequencies, while conserving the signal bearing high frequency terms of the signal. To data, the existing studies related to wavelet analysis are increasingly being applied to many different fields. In this study, we focus on several wavelet thresholding criteria or techniques to support multi-signal decomposition methods for financial time series forecasting and apply to forecast Korean Won / U.S. Dollar currency market as a case study. One of the most important problems that has to be solved with the application of the filtering is the correct choice of the filter types and the filter parameters. If the threshold is too small or too large then the wavelet shrinkage estimator will tend to overfit or underfit the data. It is often selected arbitrarily or by adopting a certain theoretical or statistical criteria. Recently, new and versatile techniques have been introduced related to that problem. Our study is to analyze thresholding or filtering methods based on wavelet analysis that use multi-signal decomposition algorithms within the neural network architectures specially in complex financial markets. Secondly, through the comparison with different filtering techniques results we introduce the present different filtering criteria of wavelet analysis to support the neural network learning optimization and analyze the critical issues related to the optimal filter design problems in wavelet analysis. That is, those issues include finding the optimal filter parameter to extract significant input features for the forecasting model. Finally, from existing theory or experimental viewpoint concerning the criteria of wavelets thresholding parameters we propose the design of the optimal wavelet for representing a given signal useful in forecasting models, specially a well known neural network models.

• The Korean Journal of Rheology
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• v.10 no.4
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• pp.202-216
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• 1998

Improved version of previous 'Orthotropic' closure approximation, termed 'ORW' has been numerically developed using new homogeneous flow data. Previous 'Orthotropic' closure approximation, i.e., ORF or ORL showed non-physical oscillation for interaction coefficient $C_1$<0.001 at simple shear flow. It also shows non-physcial oscillation and under-prediction compared with 'Distribution Function Calculation' at non-homogeneous flow of center-gated disk. These phenomena are mainly due to the flow data of 'Distribution Function Calculation' which were used for least-square optimization. ORW obtained by fitting flow data of low interaction coefficient does not show non-physical oscillation and results in reasonably good behaviors at non-homogeneous flows as well as homogeneous flows. Fitting function forms have not been found to improve overall behaviors. It has been found that considering all the eigenvalues of orientation tensor (including the third eigenvalues) might end up with a better closure approximation than just considering the first and second eigenvalues. It is, however, very important and yet difficult to select appropriate function forms of eigenvalues. Numerical simulation including coupling and in-plane velocity gradient effects were performed for injection mold filing process with a film-gated strip and a center-gated disk using ORW and various other closure approximations for comparisons. Although ORW is in excellent agreement with 'Distribution Function Calculation', the predicted results seem to have consistent error in comparison with experimental data. The diffusivity term with constant interaction coefficient might have to be further investigated in order to accurately describe orientation states.

• Journal of Korean Society of Steel Construction
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• v.24 no.6
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• pp.671-682
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• 2012

The use of sinusoidal corrugated web girder for the box-type girders and gable steel main frames has recently been increasing very much. The reasons are that the thin web of the girder affords a significant weight reduction compared with rolled beam and welded built-up girder, and that corrugation prevents the buckling failure of the web. Improvements of the automatic fabrication process makes mass production of the corrugated web and unit possible, and applications of this girder have been extended considerably. Thus, the research for the optimum design processer considering the production data is needed practically. For doing this research, we develope the discrete optimum structural design program in consideration of production list data for the research, and the program apply to the single girder under the uniform load and the concentrated load as numerical example. We consider objective function as minimum weight of the girder, and use slenderness ratio, stress of flanges and corrugated web, and the girder deflection as the constraint functions. And also the Genetic Algorithms is adopted to search the global minimum point by using the production list as a discrete design variable. Finally, to verify the optimality of the design, we conduct a comparison of the results of the discrete optimum design with those of the continuous one, and also analyze the characteristics of the optimum cross-section.

• Journal of Microbiology and Biotechnology
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• v.23 no.9
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• pp.1308-1321
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• 2013

The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monoclonal antibody (mAb) production by CHO-K1 cells. Cultures (5 ml) of microporous Cytodex 3 and macroporous CultiSpher-S carriers were performed in vented conical tubes and subsequently scaled-up (20 ml) to shake-flasks, testing combinations of different culture conditions (cell concentration, microcarrier concentration, rocking methodology, rocking speed, and initial culture volume). Culture performance was evaluated by considering the mAb production and cell growth at the phases of initial adhesion and proliferation. The best culture performances were obtained with Cytodex 3, regarding cell proliferation (average $1.85{\pm}0.11{\times}10^6$ cells/ml against $0.60{\pm}0.08{\times}10^6$ cells/ml for CultiSpher-S), mAb production ($2.04{\pm}0.41{\mu}g/ml$ against $0.99{\pm}0.35{\mu}g/ml$ for CultiSpher-S), and culture longevity (30 days against 10-15 days for CultiSpher-S), probably due to the collagen-coated dextran matrix that potentiates adhesion and prevents detachment. The culture conditions of greater influence were rocking mechanism (Cytodex 3, pulse followed by continuous) and initial cell concentration (CultiSpher-S, $4{\times}10^5$ cells/ml). Microcarriers proved to be a viable and favorable alternative to standard adherent and suspended cultures for mAb production by CHO-K1 cells, with simple operation, easy scale-up, and significantly higher levels of mAb production. However, variations of microcarrier culture performance in different vessels reiterate the need for optimization at each step of the scale-up process.

• Journal of Korean Society of Environmental Engineers
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• v.38 no.9
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• pp.521-527
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• 2016

Nanoscale zero valent iron (nZVI) has been intensively studied for the treatment of a plethora of pollutants through reductive reaction, however, the nano size should be of concern when nZVI is considered for water treatment, due to difficulties in recovery. The loss of nZVI causes not only economical loss, but also potential risk to human health and environment. Thus, the immobilization onto coarse or structured support is essential. In this study, two representative processes for nZVI immobilization on granular activated carbon (GAC) were evaluated, and optimized conditions for synthesizing Fe/GAC composite were suggested. Both total iron content and $Fe_0$ content can be significantly affected by preparation processes, therefore, it was important to avoid oxidation during preparation to achieve higher reduction capacity. Synthesis conditions such as reduction time and existence of intermediate drying step were investigated to improve $Fe_0$ content of Fe/GAC composites. The optimal condition was two hours of $NaBH_4$ reduction without intermediate drying process. The prepared Fe/GAC composite showed synergistic effect of the adsorption capability of the GAC and the degradation capability of the nZVI, which make this composite a very effective material for environmental remediation.

• Journal of the Korea Organic Resources Recycling Association
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• v.23 no.2
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• pp.47-52
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• 2015

A microbial fuel cell (MFC) is a device that can be obtained electricity from a variety of organic through the catalytic reaction of the microorganism. The MFC can be applied to various fields, and research is required to promote the performance of the microbial fuel cell for commercialization. The lower performance of an MFC is due to oxygen reduction at the cathode and the longer time of microbial degradation at anode. The MFC amount of power is sufficient but, in consideration of many factors, as a renewable energy, now commonly power density as compared to Nafion117 it is an ion exchange membrane used is PP (Poly Propylene) from 80 to about 11 fold higher, while reducing the cost to process wastewater is changed to a microporous non-woven fabric of a low cost, it may be energy-friendly environment to generate electricity. All waste, in that it can act as a bait for microorganisms, sustainability of the microbial fuel cell is limitless. The latest research on the optimization and performance of the operating parameters are surveyed and through the SSaM-GG(Smart, Shared, and Mutual- Green Growth) or GG-SSaM(Green Growth - Smart, Shared, and Mutual) as the concept of sustainable development in MFC, the middle indices are developed in this study.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.1
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• pp.579-584
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• 2017

The damper oil seal of a high-speed railway vehicle is made from nitrile butadiene rubber (NBR) in order to prevent lubricant from leaking into the damper and to stop harmful contaminants from entering the external environment while in service. Oil leakage through the seal primarily occurs from fatigue failure of the damper. Cumulative damage of the seal occurs due to the contact force between the rod and the rubber during movement due to track irregularities and cants, among other factors. Thus, the design of the oil seal should minimize the maximum principal strain at weak points. In this study, the optimal cross section of the damper oil seal was found using the multi-island genetic algorithm method to improve the durability of the damper. The optimal shape of the oil seal was derived using process automation and design optimization software. Nonlinear material properties for finite element analysis (FEA) of the rubber were determined by Marlow's model. The nonlinear FEA confirmed that the maximum principal strain at the oil leakage point was decreased 24% between the initial design and the optimum design.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.8
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• pp.147-153
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• 2020

Developments in aircraft require safety verification through flight testing for many hours with prototype design and production. The test evaluation step of performing flight tests was an important process that determines the success in the development of the system. In particular, safety development through flight tests in the armed flight test is important for the development of attack helicopters. In the development of attack helicopters, the evaluation period and cost related to the armed flight test are closely related to the required ammunition requirement. Therefore, this paper presents the amount of ammunition required for the military flight test between attack helicopter developments through an analysis of the AH-1 helicopter in a similar case and ADS-44-HDBK of military specification. The AH-1 can be used to calculate the ammunition demand by considering the exclusion of redundant firing tests and configuration differences. In the case of the machine gun-equipped configuration, approximately 10,500R was required, and approximately 324R was required in the case of a rocket-mounted configuration. In addition, if the armed integrated bench is used properly, it is expected to promote efficiently the flight test in the armed flight by identifying the possible risk factors with armed flight tests and excluding them.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.5
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• pp.364-370
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• 2009

With time, the stable management of turbidity is becoming more important in the water treatment process. So optimization of flocculation is important for the improvement of the sedimentation efficiency. we evaluated the hydrodynamic behavior in the rotation direction (clock-wise, counterclock-wise) of the flocculator in the flocculation basin using Computational Fluid Dynamics (CFD). The results of the CFD simulation, in cases where flocculators rotate in a clockwise direction, a stronger flow is formed near the surface of the water where the rotating direction and current of flow correspond. The variance and standard deviation of the flux are about 8.5 and 2.9 respectively. In contrast, in the case of a counterclockwise direction, a stronger flow is formed near the bottom of the basin. The variance and standard deviation of the flux are about 5.3 and 2.3, respectively. The effluent flux is affected more by the third flocculator spin than the first and second flocculator spins. The third flocculator spinning in the counterclockwise direction is better for the uniform flow of the sedimentation basin than the third flocculator spinning in the clockwise direction