• Journal of Korean Society of Environmental Engineers
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• v.38 no.10
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• pp.535-542
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• 2016

As one of the most cost-effective methods for surface emission measurements, flux chamber method has been used worldwide. It can be classified into two types: SFC (with slope method) and DFC (with steady-state method). SFC (static flux chamber) type needs only simple equipment and is easy to handle. However, the value of flux might vary with SFC method, because it assumes that the change of concentration in chamber is linear with time. Although more specific equipments are required for DFC (dynamic flux chamber) method, it can lead to a constant result without any ambiguity. We made a self-designed DFC using a small and compact kit, which recorded good sample homogeneity (RSD < 5%) and recovery ( > 90%). Relative expanded measurement uncertainty of this improved DFC method was 7.37%, which mainly came from uncontrolled sweep air. The study shows that the improved DFC method can be used to collect highly reliable emission data from large landfill area.

• Journal of Korean Society of Environmental Engineers
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• v.39 no.8
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• pp.478-488
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• 2017

Stormwater filtration is widely used for the urban runoff treatment. However, intensive maintenance and lack of information about the performance have resulted in an increased need of proper evaluation. In this study, the performance of an upflow stormwater runoff filtration system, consisting of a supporting unit and a filtration unit filled with a ceramic media, was investigated. The maximum head loss increase was about 3 cm under the suspended solid (SS) load of $30kg/m^2$ and the SS removal was more than 96%, when the filtration velocity was 20-40 m/h. The head loss and the porosity of the media can successfully be described by a power model. It was confirmed that the a significant amount of SS can effectively be removed at supporting unit, minimizing SS load to the filter media bed. Several backwashing strategies have been tested to establish the optimum condition. It was found that the stagnant water discharge is important to minimize the SS release immediately after backwashing. Also, the filter bed loaded with $400-450kg/m^2$ SS can almost completely be washed to reduce the head loss to the that of empty bed. The results in this study indicate that the upflow ceramic media filter is an excellent alternative to stormwater treatment, with high SS removal and long lifespan.

• Journal of the Korean Society of Hazard Mitigation
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• v.10 no.1
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• pp.89-102
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• 2010

Future uncertainty on water demand caused by future climate condition and water consumption leads a difficulty to determine the reservoir operation rule for supplying sufficient water to users. It is, thus, important to operate reservoirs not only for distributing enough water to users using the limited water resources but also for preventing floods and drought under the unknown future condition. In this study, the reservoir storage is determined in the first stage when future condition is unknown, and then, water distribution to users and river stream is optimized using the available water resources from the first stage decision using 2-stage stochastic linear programming (2-SLP). The objective function is to minimize the difference between target and actual water storage in reservoirs and the water shortage in users and river stream. Hedging rule defined by a precaution against severe drought by restricting outflow when reservoir storage decreases below a target, is also applied in the reservoir operation rule for improving the model applicability to the real system. The developed model is applied in a system with five reservoirs in the Han River basin, Korea to optimize the multi-reservoir system under various future water demand scenarios. Three multi-purposed dams - Chungju, Hoengseong, and Soyanggang - are considered in the model. Gwangdong and Hwacheon dams are also considered in the system due to the large capacity of the reservoirs, but they are primarily for water supply and power generation, respectively. As a result, the water demand of users and river stream are satisfied in most cases. The reservoirs are operated successfully to store enough water during the wet season for preparing the coming drought and also for reducing downstream flood risk. The developed model can provide an effective guideline of multi-reservoir operation rules in the basin.

• Applied Biological Chemistry
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• v.37 no.4
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• pp.303-309
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• 1994

To develop the treatment process of swine wastewater using Rhodopseudomonas palustris KK14 with high utilizable ability of organic acids, some operating conditions were investigated and optimized in flask-scale and laboratory-scale reactors. The optimal operating conditions in photosynthetic bacteria (PSB) reactor of semi-continuous type were obtained at HRT 6 day, 5% (v/v/day) seeding rate of PSB sludge and 10% (v/v/day) returning rate of PSB return sludge. Under the above operating condition, COD level of the wastewater (initial COD: 10 g/l) was reduced to about 1.7 g/l after 4 days treatment and MLSS was held constant at $4{\sim}5\;g$ per liter. In laboratory-scale process consisted of 5.2 l anaearobic digestion reactor and 15 l PSB reactor, the total removal rates of COD and BOD were increased to 95% and 96% by the continuous operation for 5.36 days, respectively, showing $3kg\;COD/m^3/day$ COD loading rate and 1.1 Kg COD/Kg MLSS/day sludge loading rate in PSB reactor. The offensive odor was considerably removed through the treatment process of swine wastewater.

• Applied Biological Chemistry
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• v.46 no.4
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• pp.291-298
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• 2003

A bacterial strain producing a high level of an extracellular phytase was isolated from livestock waste water, identified as a strain of Pseudomonas fragi and designated as Pseudomonas fragi Y9451. Under the phytase production medium, the activity of phytase reached the highest level after 120 hours of incubation. On the effect of carbon sources on the phytase production, the most favorable carbon source for phytase production was fructose. As for the effect of nitrogen sources, high levels of phytase activity were detected in the medium containing nutrient broth as the nitrogen source. Free $PO_4^{3-}$ inhibited phytase production with increasing concentration of $KE_2PO_4$ and phytate in the media. The addition of $CaCl_2$ and $MgSO_4$ also resulted in the inhibition of phytase production. To investigate the effect of aeration on the phytase production, different volumes of culture broth in Erlenmeyer flasks were incubated in rotary shaker at the speed of 200 rpm. As a result, a high level of phytase activity was detected at small volume of culture broth as compared to larger volume because of its more aerobic condition.

• Journal of Applied Biological Chemistry
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• v.53 no.4
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• pp.225-231
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• 2010

To understand the inhibitory activity with changing hydroxyl substituents ($R_l-R_9$) of polyhydroxy substituted 2-phenyl-l,4-benzopyrone analogues (1-25) against tyrosinase (PDB ID: oxy-form; 1WX2), molecular docking and the three dimensional quantitative structure-activity relationships (3D-QSARs: Comparative molecular field analysis (CoMFA) & Comparative molecular similarity indices analysis (CoMSIA)) were studied quantitatively. The statistically best models were CoMFA 1 and CoMSIA 1 model from the results. The optimized CoMSIA 1 model with the sensitivity of the perturbation and the prediction produced ($dq^2'/dr_{yy'}^2$=1.009 & $q^2$=0.51l) by a progressive scrambling analysis were not dependent on chance correlation. The inhibitory activities with optimized CoMSIA 1 model were dependent upon electrostatic factor (51.4%) of substrate molecules. Contour mapping the 3D-QSAR models to the active site of tyrosinase provides new insight into the interaction between tyrosinase as receptor and 2-phenyl-l,4-benzopyrone analogues as inhibitor. Therefore, the results will he able to apply to the optimization of a new potent tyrosinase inhibitors.

• Analytical Science and Technology
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• v.16 no.2
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• pp.102-109
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• 2003

Microwave digestion and solid-state extraction were studied for determination of trace aluminum{Al(III)} in human urine samples. A mixed acid of nitric acid and hydrogen peroxide was added to urine samples, organic materials were destructed in a home microwave oven and dried in a drying oven. The dried residues were dissolved in a sulfuric acid solution. The solution was eluted through a XAD-4 resin column adsorbed with 8-hydroxyquinoline(Oxine, HQ). Al(III)-8-hydroxyquinolinate complex was formed in the column and eluted with 0.5 M nitric acid solution. The Al(III) eluted was determined by graphite atomic absorption spectrophotometry. Various experimental conditions of followings were investigated for the optimization : the type of acid to dissolve the residues, the amount of HQ adsorbed on the resin, the pH of sample solutions, the type and concentration of acid to elute the complex from column and so on. The contents of Al(III) in real samples were determinated by a calibration curve method. The recovery in standard spiked samples was 94~101% and the detection limit of this procedure was 0.05 ng/mL.

• Journal of Energy Engineering
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• v.21 no.4
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• pp.402-410
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• 2012

A two-loop Rankine cycle for engine waste heat recovery of gasoline vehicle has been investigated. Water-steam cycle as a high-temperature (HT) loop for exhaust gas heat recovery and R-134a cycle as a low-temperature (LT) loop for both heat recovery of the engine coolant and the residual heat from the HT loop were considered. Energy and exergy analysis was performed to investigate the performance of the system. Because two volumetric expanders are used for the HT and LT loop, the sizes of two expanders are very important for the optimization of the system. The effects of pressure ratio of the HT loop, considering the size of the HT expander, and the condensation temperature of LT loop on the performance of the system at a target engine condition were investigated. This study shows that about 20% of additional power from the engine waste heat recovery can be obtained at the target engine condition.

• The Journal of Engineering Geology
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• v.30 no.3
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• pp.269-278
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• 2020

In this study, the effects of groundwater artificial recharge through vertical wells in the upper small basin are preliminarily evaluated by using field injection test and a 3-D numerical model. The injection rate per well in a model is set to 20, 37.5, 60, and 75 ㎥/day based on the results of field injection test, groundwater levels, and hydraulic conductivities estimated from particle size analysis, and a numerical model using MODFLOW is conducted for 28 cases, which have diverse injection intervals, in order to estimated the changes of groundwater level and water balance after injection. Groundwater level after injection does not show a linear relationship with the injection rate per well, and the cumulative effect of artificial recharge decreases and the timing of maximum water level rise is shortened as the injection interval becomes longer. In four cases of continuous injection with total injection rate of 1,200 ㎥, it is revealed that the recharge effect is analyzed as 36.5~65.3% of the original injection rate. However, it will be more effective if the artificial recharge system combined with underground barrier is introduced for the longer pumping during a long and severe drought. Additionally, it will be possible to build a stable artificial recharge system by an establishment of efficient scenario from recharge to pumping as well as an optimization of recharge facilities.

• Journal of Embryo Transfer
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• v.15 no.3
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• pp.247-253
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• 2000

This study was undertaken to optimize enucleation and reconstitution methods for the production of cloned mice by somatic cell nuclear transfer Outbred ICR mouse oocytes at the metapahse- II stage were retrieved from female mice superovulated by PMSG and hCG. In Experiment 1, oocytes were enucleated in medium supplemented with cytochalasin B (CCB) of 3 levels (0, 7.5 or 15 $\mu\textrm{g}$/mL), and higher rate of encleation was obtained at 7.5 and 15 $\mu\textrm{g}$/mL than at $\mu\textrm{g}$/mL. In Experiment 2, oocytes enucleated in 7.5 $\mu\textrm{g}$/mL CCB-containing medium were reconstituted with different types of somatic cell by following methods; 1) cumulus cells by direct cell injection, 2) cumulus cells by electric fusion (1.25 kV/cm, 2 pulses for each 70 $mutextrm{s}$) or 3) STO cells by the electrofusion. Electrofusion of STO cells with enucleated oocytes yielded the greatest (P<0.05) rate of reconstitution without lysis (76%) than any other combinations. Although significant decrease in the rate of somatic cell introduction was found, the electrofusion of cumulus cells yielded better rate of reconstitution than direct injection (0 vs. 18%). In Experiment 3, the duration of electric stimulation for the fusion was changed to either 50 $mutextrm{s}$ or 90 $mutextrm{s}$, but no significant improvement of reconstitution efficacy was obtained. In conclusion, this study showed that ICR mouse oocytes could be used for the production of reconstituted oocytes and a fusion method of 1.25 KV/cm with 2 pulses using 570 cell was the optimal.