• 생명과학회지
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• 제14권2호
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• pp.245-251
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• 2004

본 연구는 streptozotocin으로 당뇨병을 유도하는 조건들을 농도에 따른 변화를 관찰하고, 적절한 조건에서 당뇨쥐에서의 저강도의 treadmill 운동이 체중과 혈중의 당, 인슐린 및 지질의 변화에 미치는 영향을 알아보고자 실시하였으며, 연구결과를 요약하면 다음과 같다. 실험 I에서 STZ 주사량이 40 mg/mg으로 높아질수록 정상군에 비해 혈당이 유의하게 증가하고, 반대로 체중과 인슐린 농도는 상대적으로 감소하였다. 실험II에서 STZ 주사를 한 모든 군에서 혈당이 유의하게 증가하지만 BE군은 C군에 비해 그 증가율이 유의하게 억제되었으며, 운동이 인슐린 농도와 혈중지질 농도를 정상적으로 유도하는 것으로 나타났다. 특히, 당뇨 유발 전부터 운동을 실시한 경우 그 효과가 더욱 유의하게 나타났으며, 앞으로 당뇨 유발의 정도와 운동 빈도 및 강도와 관련된 더욱 많은 연구가 이루어져야 할 것으로 사료된다.

• The Korean Journal of Pain
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• 제23권3호
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• pp.190-197
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• 2010

Background: Conventional spinal saddle block is performed with the patient in a sitting position, keeping the patient sitting for between 3 to 10 min after injection of a drug. This amount of time, however, is long enough to cause prolonged postoperative urinary retention. The trend in this block is to lower the dose of local anesthetics, providing a selective segmental block; however, an optimal dose and method are needed for adequate anesthesia in variable situations. Therefore, in this study, we evaluated the question of whether only 1 min of sitting after drug injection would be sufficient and safe for minor anorectal surgery. Methods: Two hundred and sixteen patients undergoing minor anorectal surgery under spinal anesthesia remained sitting for 1 min after completion of subarachnoid administration of 1 ml of a 0.5% hyperbaric bupivacaine solution (5 mg). They were then placed in the jack-knife position. After surgery, analgesia levels were assessed using loss of cold sensation in the supine position. The next day, urination and 11-point numeric rating scale (NRS) for postoperative pain were assessed. Results: None of the patients required additional analgesics during surgical manipulation. Postoperative sensory levels were T10 [T8-T12] in patients, and no significant differences were observed between sex (P = 0.857), height (P = 0.065), obesity (P = 0.873), or age (P = 0.138). Urinary retention developed in only 7 patients (3.2%). In this group, NRS was $5.0{\pm}2.4$ (P = 0.014). Conclusions: The one-minute sitting position for spinal saddle block before the jack-knife position is a safe method for use with minor anorectal surgery and can reduce development of postoperative urinary retention.

• Fisheries and Aquatic Sciences
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• 제7권2호
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• pp.70-75
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• 2004

The CMV promoter driven lacZ reporter gene (pcDNA-lacZ) was constructed and used for DNA immunization study. The expression of the lacZ gene was confirmed in vitro using RTG-2 cell line before using for in vivo study in olive flounder (Paralichthys olivaceus). In the dose response study, the maximum expression of the lacZ gene was found in the group injected with 5 ${\mu} g$ of the plasmid DNA. Kinetic study showed a significantly increased expression of $\beta-galactosidase$ gene at 7 days after injection. Effects of DNA vaccine on specific and nonspecific immune responses such as antibody and NO production were studied and the significant effect was found in olive flounder injected with 10 and 15 ${\mu} g$ DNA (sub optimal dose for lacZ gene expression). Two pro inflammatory cytokine genes, $IL-l\beta$ and $TNF-\alpha$, were also found to be up regulated in the muscle injected with the plasmid, suggesting an induction of local inflammatory response.

• Journal of Veterinary Science
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• 제21권4호
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• pp.55.1-55.11
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• 2020

Background: Computed tomography urography (CTU), based on the excretion of contrast medium after its injection, allows visualization of the renal parenchyma and the renal collecting system. Objectives: To determine the optimal contrast medium dose allocation ratio to apply in split-bolus CTU in dogs. Methods: This prospective, experimental, exploratory study used 8 beagles. In 3-phase CTU, unenhanced-, nephrographic-, and excretory-phase images were obtained with a single injection of 600 mg iodine/kg iohexol. In split-bolus CTU, two different contrast medium allocation ratios (30% and 70% for split CTU 1; 50% and 50% for split CTU 2) were used. Unenhanced phase image and a synchronous nephrographic-excretory phase image were acquired. Results: Although the attenuation of the renal parenchyma was significantly lower when using both split CTUs than the 3-phase CTU, based on qualitative evaluation, the visualization score of the renal parenchyma of split CTU 1 was as high as that of the 3-phase CTU, whereas the split CTU 2 score was significantly lower than those of the two others. Artifacts were not apparent, regardless of CTU protocol. The diameter and opacification of the ureter in both split CTUs were not significantly different from those using 3-phase CTU. Conclusions: Split-bolus CTU with a contrast medium allocation ratio of 30% and 70% is feasible for evaluating the urinary system and allows sufficient enhancement of the renal parenchyma and appropriate distention and opacification of the ureter, with similar image quality to 3-phase CTU in healthy dogs. Split-bolus CTU has the advantages of reducing radiation exposure and the number of CT images needed for interpretation.

• 한국미생물·생명공학회지
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• 제21권3호
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• pp.247-255
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• 1993

Antitumor activity of Lactobacillus casei YIP 9018(LC9018) was studied in mice by using sarcoma 180(S-180) and Lewis lung carcinoma (3LL). Following the eatablishment of in vivo tumor models such as ascites form S-180, solid form S-180 and 3LL for estimating antitumor activity of Lactobacilli, optimal dose and injection route of heat-killed LC9018 for supperssion of local tumor were examined. Administration of 100ng/mouse of LC9018 significantly inhibited the growth of ascites form S-180, solid form S-180 and 3LL.

• 한국분말재료학회지
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• 제15권6호
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• pp.466-470
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• 2008

Experiments with a URT-0.5 accelerator (0.5 MeV, 50 ns, 1 kW) generating a nanosecond electron beam for irradiation of silver nitrate in various liquid solutions (water and toluene) were performed with the aim of producing silver nanopowders. A radiochemical reaction allows making weakly agglomerated pure Ag powders with particles of 10-15 nm and 30-50 nm in size by irradiation in toluene and water respectively. The injection of the nanosecond electron beam energy to the solution is optimal. As the absorbed dose increases, the output of the radiochemical reaction does not grow, but more agglomerated powders are synthesized.

• Journal of Chest Surgery
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• 제53권4호
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• pp.178-183
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• 2020

During esophagectomy and esophagogastrostomy, the prediction of anastomotic leakage relies on the operating surgeon's tactile or visual diagnosis. Therefore, anastomotic leaks are relatively unpredictable, and new intraoperative evaluation methods or tools are essential. A fluorescence imaging system enables visualization over a wide region of interest, and provides intuitive information on perfusion intraoperatively. Surgeons can choose the best anastomotic site of the gastric tube based on fluorescence images in real time during surgery. This technology provides better surgical outcomes when used with an optimal injection dose and timing of indocyanine green.

• Journal of Korean Neurosurgical Society
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• 제65권2호
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• pp.180-185
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• 2022

Objective : Drug-eluting stents and balloons are occasionally used to reduce restenosis in medically intractable intracranial atherosclerotic stenosis. The authors aimed to determine whether such drugs can cause neurotoxicity due to local effects in a rat model. Methods : Intra-arterial catheters were placed in the right common carotid artery of rats. Mannitol was injected to transiently open the brain-blood barrier (BBB), followed by high-dose drug (paclitaxel and rapamycin) injection. The optimal time interval of transient BBB opening for maximal drug penetration was determined to be 10 minutes. Paclitaxel and rapamycin were intra-arterially administered in various doses. All the rats were neurologically evaluated, and their brain tissues were histologically examined. Results : Neither neurological deficits nor histological abnormalities were observed in all the rats. Conclusion : Paclitaxel and rapamycin did not cause neurotoxicity in a rat model with transient BBB opening.

• 대한의용생체공학회:의공학회지
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• 제20권2호
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• pp.173-182
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• 1999

조영 증강제를 이용한 나선식 CT는 간 질환을 진단하는데 있어 중요한 역할을 하고있음에도 불구하고 진단의 효율을 최적화하는 프로토콜은 명확하게 알려져 있지 않다. 따라서 나선식 CT에서의 간암-간 대조 곡선을 모의 실험(simulation) 하여 다양한 요소들이 시간-조영 곡선에 어떻게 영향을 미치는지 파악하고, 또한 CT 검사 전에 모의 실험을 하여 이론적으로 최적의 스캔을 할 수 있도록 하기 위하여 약동학(pharmacokinetics)에 기초한 compartment model을 구성하였다. 간암, 간, 대동맥 및 간문맥 등을 각 구획(compartment)으로 설정하여 각 구획에서의 미분방정식을 얻은 후 적분하여 Hounsfield unfit 값을 조영제 주입 후 시간의 함수로 얻었으며 각 구획의 시간-조영 곡선을 출력하였다. 구현한 프로그램에서는 간암의 크기 및 종양 혈관의 분포 등과 같은 간암의 성질, 간경화의 정도에 따른 간 혈관 공급의 양상 및 조영제의 부피, 농도, 주입 속도 등의 조영제 주입 방법, 환자의 몸무게, 키 등의 환자의 신체 계수, 그리고 심박출량 등의 환자의 혈역학적 계수 등을 입력 받아 간암을 비롯한 각 구획의 시간-조영 곡선 및 간-간암 대조 곡선을 출력할 수 있도록 하였다. 모델링을 통해 얻은 조영 증강 곡선은 같은 환경하에서 얻은 24명의 환자 데이터와 비교하여 유사한 결과를 얻을 수 있었으며, 조영 증강제 주입 방법의 변화가 간암-간 대조 곡선에 미치는 영향을 비교할 수 있었다.

• Nuclear Engineering and Technology
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• 제56권6호
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• pp.2153-2162
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• 2024

The purpose of this study is to develop a high-energy neutron shielding material applied in proton therapy environment. Composite shielding material consisting of 10.00 wt% boron carbide particles (B₄C), 13.64 wt% surface-modified cross-linked polyethylene (PE), and 76.36 wt% tungsten particles were fabricated by hot-pressure sintering method, where the optimal ratio of the composite is determined by the shielding effect under the neutron field generated in typical proton therapy environment. The results of Differential Scanning Calorimetry measurements (DSC) and tensile experiment show that the composite has good thermal and mechanical properties. In addition, the high energy-neutron shielding performance of the developed material was evaluated using cyclotron proton accelerator with 100 MeV proton. The simulation shows a 99.99% decrease in fast neutron injection after 44 cm shielding, and the experiment result show a 99.70% decrease. Finally, the shielding effect of replacing part of the shielding material of the proton therapy hall with the developed material was simulated, and the results showed that the total neutron injection decreased to 0.99‰ and the neutron dose reduced to 1.10‰ before the enhanced shielding. In summary, the developed material is expected to serve as a shielding enhancement material in the proton therapy environment.