• Journal of Life Science
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• v.14 no.1
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• pp.51-56
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• 2004

The polysaccharide isolated from Phellinus species has been known as a folk remedy, including antitumor and immune-stimulating activities. However, there are lacks of knowledge about mycelial growth and exopolysaccharide (EH) production in its submerged culture. We investigated the optimal conditions on mycelial growth and EPS production in Phellinus baumii. The optimal temperature and initial pH for mycelial growth and EPS production in shake flask culture of P. baumii were proved to be 3$0^{\circ}C$ and pH 5.0, respectively. In case of carbon source, cellobiose and maltose were highly efficient for mycelial growth and fructose and mannitol were also relatively favorable for EPS production. Yeast extract was the most suitable nitrogen source for mycelial growth and EPS production. The composition of optimal culture medium was determined to be fructose 20 g/L, yeast extract 20 g/L, and $CaCl_2$ 0.55 g/L, respectively. Under the optimal culture condition, the maximum mycelial biomass and EPS achieved in a 5-L stirred-tank fermenter were 17.43 g/L and 3.6 g/L, respectively. It was found that the EPS was a glycoprotein onsisted of mainly arginine (14.1%) and glycine (12.0 %) in protein moiety and mainly mannose (48.7%) and arabinose (38.4%) in carbohydrate moiety.

• Korean Journal of Plant Resources
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• v.15 no.2
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• pp.135-143
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• 2002

The results of examining characteristics of mycelial growth and culture condition for determining the condition of artificially culturing Inonotus obliqua (chaga) are as follows. 1) Mycelial growth and density oft, oblique were the highest in the medium of BDA (Birch Dextrose Agar; 66.3mm/10d) followed by the order of GDA, PDA, CDA, PODA, ODA, YM, MCM, MEA (pH 7.0), CHA, and MEA (pH 4.7). 2) Optimal temperature for the mycelial growth and density of 1. obliqua were shown to be 30$^{\circ}C$, but the mycelia were dead at 40$^{\circ}C$. the mycelial growth and density of KNAC3005 strains was the highest at 30$^{\circ}C$(66.3mm/10d) followed by the order of 25, 20, 15, 35, 10, and 5$^{\circ}C$. 3) Optimal pH for the mycelial growth and density were revealed to be 6.0 (88.4mm/10d). Above or below pH 6.0, the mycelial growth and density were shown to be retarded. 4) Optimal carbon, nitrogen and organic acid sources for the spawn growth of 40 were maltose (331mg/25$m\ell$/15d), peptone (347mg/25$m\ell$/15d), and glutamic acid (357mg/25$m\ell$/15d), respectively. Optimal level of biotin was 370mg/15d and optimal C/N ratio was 40.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.6
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• pp.487-494
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• 2006

The effects of water temperature, salinity and irradiance on the growth of harmful algae Chattonella marina isolated from Gamak Bay in South Sea, Korea were investigated. C. marina was able to grow in temperatures of $15-30^{\circ}C$ and salinities of 10-35 psu. Maximum specific growth rate (0.64/day) was observed with combination of $25^{\circ}C$ and 25 psu. Optimal growth (${\ge}70%$ of maximum specific growth rate) was obtained with all salinities of the above $20^{\circ}C$. This result indicated that C. marina is a stenothermal of the high water temperature and euryhaline organism. C. marina was did not grow at irradiance ${\le} 10{\mu}mol$ photons/($m^2\;s$). Photoinhibition did not occur at $300{\mu}mol$ photons/($m^2\;s$), which was the maximum irradiance used in this study. The irradiance-growth curve was described as ${\mu}=0.78(I-11.4)/(I+34.1)$ at $25^{\circ}C$ and 25 psu. The half-saturation photon flux density (PFD) ($K_s$) was $56.9{\mu}mol$ photons/($m^2\;s$) and compensation PFD ($I_c$) was $11.4{\mu}mol$ photons/($m^2\;s$). The result of the present study indicate that C. marina has advantage physiological characteristic to the interspecific competition at the embayment and costal areas of South and West Sea, Korea in summer.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.3
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• pp.290-297
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• 2011

The phenology of Campylaephora hypnaeoides J. Agardh and optimal conditions for carpospore release, growth and reproduction were examined in the field and in the laboratory from January to December 2007. In the field population of C. hypnaeoides, approximately 50% of the plants were vegetative during the study period. Additionally, the percentages of carposporophytes and tetrasporophytes were maximal in April (37%) and June (57%), respectively. Maximum growth in plant length, dry weight, and hook number coincided with the tetrasporophyte reproductive peak in the field. In culture, carpospore release, sporeling growth and reproduction were affected by environmental factors such as daylength, temperature, and salinity. The liberation of carpospores was maximum under continuous light and at a combination of $15^{\circ}C$ and $10\;{\mu}mol$ photons $m^{-2}\;s^{-1}$. Maximum growth of tetrasporophyte sporelings occurred at a combination of $20\;{\mu}mol$ photons $m^{-2}\;s^{-1}$ of constant light and $25^{\circ}C$. However, the growth of gametophyte sporelings was maximal under $40\;{\mu}mol$ photons $m^{-2}\;s^{-1}$ of constant light and in a combination of $20^{\circ}C$ and 35 psu. The tetrasporophyte sporelings were grew faster than gametophytes, indicating that gametophyte- and tetrasporophyte-sporelings have different physiological responses to irradiance and temperature. Tetrasporangial branches and cystocarps of C. hypnaeoides were produced from carpospores and tetraspores within 1 month, and they were stimulated at high temperature and irradiance levels. In conclusion, C. hypnaeoides should be seeded using carpospores during early winter (November-December) because cystocarps are easily identified by fishermen, and tetrasporophytes grow faster than gametophytes.

• Mycobiology
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• v.33 no.1
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• pp.12-14
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• 2005

The culture conditions for the enhanced mycelial growth of Ramaria botrytis was investigated. The optimal temperature and pH for the mycelial growth were $24^{\circ}C$ and 5.0, respectively. It was shown that starch was best of several carbon sources in Czapek-Dox medium as a minimal medium for the enhanced mycelial growth. Organic nitrogen sources were better than inorganic ones for mycelial growth. The appropriate vitamin and mineral salt were biotin and FeCl3, respectively. When this strain was cultured with $FeCl_3$ for 30 days, 19.23 g/l of dry mycelium of R. botrytis was obtained.

• Journal of Mushroom
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• v.14 no.1
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• pp.6-13
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• 2016

This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and $35^{\circ}C$, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and $MgSO_4{\cdot}7H_2O$ and $FeSO_4{\cdot}7H_2O$ as mineral salts.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.3
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• pp.257-262
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• 2017

This study was conducted to investigate the growth and skin color of red- and white-colored fancy carp Cyprinus carpio var. koi juvenile reared at different water temperatures (21, 24, 27, and $30^{\circ}C$) for eight weeks. Two experimental diets were prepared to contain 10% Arthrospira platensis or 10% Arthrospira pacifica powder. After the feeding trial, survival was 100% in all group. Weight gain of fish reared at 24, 27, and $30^{\circ}C$ was higher than $21^{\circ}C$ group. Feed efficiency was not affected by water temperature and dietary Arthrospira sp. Daily feed intake tended to increase with water temperature. The $a^*$ value of skin coloration was affected by Arthrospira sp., but not by water temperature. Fish fed the diet containing A. pacifica powder reared at $27^{\circ}C$ had the highest $a^*$ value among the groups. Total carotenoids were highest in fish fed the diet containing A. pacifica powder reared at $21^{\circ}C$. Based on the results of this study, we conclude that 24-$30^{\circ}C$ water temperature was the optimal range for growth, and dietary inclusion of A. pacifica improved the skin redness of red- and white-colored fancy carp reared at $27^{\circ}C$.

• Microbiology and Biotechnology Letters
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• v.28 no.6
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• pp.329-333
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• 2000

Alkaline bacterium producing a high pro-tease activity at low temperature was isolated by using enrichment culture from soil samples and identified as Bacil-lus sp. WRD-1 Cell growth was maximal at 10 hours and the optimal initial pH and culture time of culture condition for enzyme production was pH 7 and 10 hours, respectively. Temperature range of high enzyme activity were $10~40^{\circ}C$. The optimal pH and temperature for the enzyme activity were pH9 and $30^{\circ}C$.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.9 no.5
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• pp.504-509
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• 1999

In-situ cleaning and subsequent silicon epitaxial film growth were performed in a load-locked reactor equipped with Hg-grid UV lamp and PBN heater to obtain the smooth and contaminant-free underlying surface and develop low-temperature epitaxial film growth process. The removals of organic and native oxide were investigated using UV-excited $O_2$ and $NF_{3}/H_{2}$, and the effect of the surface condition was examined on the quality of silicon epitaxial film grown at temperature as low as $750^{\circ}C$. UV-excited gas phase cleaning was found to be effective in removing the organic and native oxide successfully providing a smooth surface with RMS roughness of 0.5$\AA$ at optimal condition. Crystalline quality of epitaxial film was determined by smoothness of cleaned surface and the presence of native oxide and impurity. Crystalline defects such as dislocation loops or voids due to the surface roughness were observed by XTEM.

• Mycobiology
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• v.28 no.4
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• pp.165-170
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• 2000

This study was carried out to obtain the basic data for artificial culture of veiled lady mushroom (Dictyophora spp). The optimal conditions for the mycelial growth were $25^{\circ}C$ and pH 5.0 for all isolates except the optimal temperature of $30^{\circ}C$ for D. echinovolvata ASI 32002 and Phallus rugulosus. The optimal medium for Dictyophora spp. was PBA (potato bamboo sawdust extract agar) medium. The strain ASI 32002, D. echinovolvata, grew faster than. D. indusiata ASI 32003 and Phallus rugulosus ASI 25007 on the medium. Carbon sources such as glucose, maltose and inuline were favorable for stimulating a mycelial growth of the two strains of ASI 32002 and ASI 32003. Asparagine and glutamine appeared to be favorable to the strain ASI 32002 and ASI 32003, where as alanine, one of nitrogen source also favorable to the strain ASI 32002. The optimum C/N ratio of the two isolates of ASI 32002 and ASI 32003 was about 25 : 1 when 2% glucose as carbon source was mixed with the basal medium. While, in the case of 4% as carbon source, the optimum C/N ratio was about 30 : 1.