• Applied Microscopy
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• v.46 no.4
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• pp.188-192
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• 2016

Proper sample preparation prior to microscopy is necessary for maintaining the components of tissues in a state as close to a living state as possible. For optimal preservation of biological samples, the sampling conditions are as important as the fixation itself. Various factors influence the selection and fixation efficiencies of a fixative, including sample size, osmolarity, pH, penetration rate and depth, fixative temperature, fixation time, fixative concentration, fixative amount, and retention time. Therefore, several factors for selecting and administering fixation procedures are evaluated pertaining to optimal sample preparation for transmission electron microscopy.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.59 no.5
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• pp.882-889
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• 2010

This paper presents a method of finding the optimal operating point minimizing a given objective function with 3 phase power flow equations and operational constraints, called 3 phase optimal power flow (3POPF). 3 phase optimal power flow can provide operation and control strategies for the distribution systems with distributed generation assets, which might be frequently in unbalanced conditions assuming that high penetration rate of renewable energy sources in the systems. As the solution technique for 3POPF, this paper adopts a simulation-based method of particle swarm optimization (PSO). In the PSO based 3POPF, a utility function needs to be defined for evaluation of the degree in operational improvement of each particle's current position. To evaluate the utility function, in this paper, NR-based 3 phase power flow algorithm was developed which can deal with looped distributed generation systems. In this paper, illustrative examples with a 5-bus and a modified IEEE 37-bus test systems are given.

• Proceedings of the KIEE Conference
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• 2009.07a
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• pp.482_483
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• 2009

This paper presents a method of finding the optimal operating point minimizing a given objective function with 3 phase power flow equations and operational constraints, called 3 phase optimal power flow. 3 phase optimal power flow can provide operation and control strategies for the distribution systems with distributed generation assets, which might be frequently in unbalanced conditions assuming that high penetration rate of renewable energy sources in the systems. As the solution technique, this paper adopts a standard particle swarm optimization (PSO).

• Journal of Korean Tunnelling and Underground Space Association
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• v.14 no.5
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• pp.485-501
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• 2012

This study aims to experimentally investigate ground settlement and ground movement around the square pipe by its penetration in sandy ground. A series of laboratory model tests were carried out with a small-scale auger equipment for penetration of a square pipe as well as a newly designed test box with a sand raining equipment. From the experiments, it is shown that a square pipe induces ground movement evenly around it in a low overburden condition. However, as the overburden becomes higher, ground movement by a square pipe is concentrated mainly above it. Especially, horizontal strain above the square pipe was mainly dominated by its penetration. In addition, sand surface movement is the smallest in case of the dimensionless penetration rate equal to 0.2. When its penetration rate of the square pipe is fixed, the rotation speed of auger controls surface movement whether it is settlement or heaving. Therefore, the selection of an optimal dimensionless rate for the square pipe is a key design factor to minimize ground settlement in a trenchless construction.

• Journal of Welding and Joining
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• v.25 no.5
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• pp.64-71
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• 2007

The aim of this paper is to investigate the effects of welding parameters on the weld shape in seal-welding of STS304L capsule for manufacturing a radioisotope source which is widely used in nondestructive testing of metal structures using gamma ray. Pulsed gas tungsten arc (Pulsed GTA) welding is performed for thin cross sectional area of the capsule. Seven welding parameters including current waveform parameters and arc length etc. are selected as main process parameters using design of experiment. The weld shape such as bead width, penetration depth, weld area, aspect ratio and area rate is investigated to assess the effects of welding parameters. As results, the combination of pulse duty/welding speed largely affects on bead width, penetration depth, area and aspect ratio. Finally, it is concluded that the key parameters are the combination of pulse duty/welding speed, base current and arc length, and their optimal conditions are 50%/1.77mm/s, 6.4A and 1 mm.

• Journal of Distribution Science
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• v.17 no.4
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• pp.51-57
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• 2019

Purpose - This study aims to improve the distribution structure of the OLED market and develop cost-effective optimization techniques. Specifically, it is a study on the optimization of ferric chloride to improve the etch of SUS MASK for OLED. Research design, data, and methodology - Applying the optimal conditions of the experiment, the final confirmation was evaluated for improvement by the Process Capability Index (Cpk). It is possible to derive social performance such as improvement of precision of SUS MASK manufacturing, economic performance such as defect rate, reduction of waste generation and treatment cost, technological achievement such as SUS MASK production technology, improvement of profit structure of technology development and process improvement do. Results - The improvement of the Cpk before the improvement was made was confirmed to be 0.57% with a defect estimate of 25.07% with a failure estimate of 0.57% after the improvement, and 8.84% with a failure estimate of 0.57% level after the improvement. Conclusions - If the conclusions obtained from the specimen experiment are applied to the manufacturing process of SUS MASK, it will be possible to expect excellent cost-effective competitiveness due to the improvement of precision and reduction of defect rate to enhance the OLED market penetration.

• YAKHAK HOEJI
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• v.58 no.3
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• pp.171-180
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• 2014

To enhance the in vitro permeation of lovastatin through excised hairless mouse and human cadaver skins, solubility was determined in various hydrophilic and lipophilic vehicles, and the effects of vehicles and penetration enhancers on the skin permeation from solution formulations were investigated. Solubility of lovastatin was highest in N-methyl-2-pyrrolidone (NMP) ($278.2{\pm}10.1$ mg/ml) and dimethyl sulfoxide (DMSO) ($162.2{\pm}9.7$ mg/ml). Among different pure vehicles used, NMP, DMSO, propylene glycol and isopropyl myristate provided some drug permeation ($6.9{\pm}1.1$, $5.9{\pm}1.6$, $3.0{\pm}0.5$ and $2.2{\pm}0.3{\mu}g/cm^2$ at 24 hr, respectively) through hairless mouse skin. The addition of oleic acid, linoleic acid and oleyl alcohol to DMSO showed the maximum permeation at around 5 v/v%, however, capric acid and caprylic acid had no enhancing effect. The increase of enhancer concentrations showed bell-shaped permeation rate, suggesting the presence of optimal concentration in lovastatin penetration. Increasing donor concentration from 10 mg/ml to 80 mg/ml in DMSO and a cosolvent of DMSO, NMP and DGME (3 : 3 : 4 v/v) did not show significant dose dependent permeation in both hairless mouse and human cadaver skins. The maximum lovastatin flux through human cadaver skin was found to be $0.87{\pm}0.46{\mu}g/cm^2$/hr with 5 v/v% linoleic acid and donor dose of 4 mg/0.64 $cm^2$ in the cosolvent. These results suggest that transdermal delivery of lovastatin would be feasible by establishing the optimal concentrations of donor dose and unsaturated fatty acids in appropriate vehicles.

• Journal of Animal Reproduction and Biotechnology
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• v.38 no.2
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• pp.70-76
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• 2023

Background: Despite considerable technological advancements, polyspermy remains a significant challenge in in vitro fertilization (IVF) procedures in pigs, disrupting normal embryonic development. Here, we aimed to determine whether optimal fertilization conditions reduce the polyspermy incidence in pigs. Methods: In vitro-matured oocytes were co-incubated with sperm according to a modified two-step culture system. Results: In the first experiment, oocytes were briefly co-incubated with sperm, washed in IVF medium, and then moved to fresh IVF medium for 5 or 6 h. Although the 6 h sperm-free cultured group had a higher penetration rate than the 5 h cultured group, the polyspermy rate significantly increased in the 6 h sperm-free cultured group. The gamete co-incubation period was either 20 or 40 min. The 40 min cultured group had a higher rate of blastocyst formation and number of total cells in blastocysts than the 20 min cultured group. In experiment 2, oocytes were inseminated with sperm separated by Pecroll treatment. Percoll treatment increased the rate of oocyte penetration and blastocyst formation compared to the control. In experiment 3, fertilized oocytes were cultured in 25 µL microdroplets (10 gametes/drop) or 500 µL (100 gametes/well) of culture medium in 4-well plates. The large volume of medium significantly reduced the number of dead oocytes and increased the rate of blastocyst formation compared to the small volume. Conclusions: Collectively, these results demonstrate that various fertilization conditions, including modified co-culture period, active sperm separation, and culture medium volume, enhance fertilization efficiency and subsequent embryonic development by decreasing polyspermy occurrence.

• Archives of Pharmacal Research
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• v.24 no.6
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• pp.572-577
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• 2001

The feasibility of skin penetration was studied for aspalatone (AM, acetylsalicylic acid maltol ester), a novel antithrombotic agent. In this studys hairless mouse dorsal skins were used as a model to select composition of vehicle and AM. Based on measurements of solubility and partition coefficient, the concentration of PC that showed the highest flux for AM across the hairless mouse skin was found to be 40%. The cumulative amount permeated at 48 h, however, appear inadequate, even when the PC concentration was employed. To identify a suitable absorption enhancer and its optimal concentration for AM, a number of absorption enhancers and a variety of concentration were screened for the increase in transdermal flux of AM. Amongst these, linoleic acid (LOA) at the concentration of 5% was found to have the largest enhancement factor (i.e., 132). However, a further increase in AM flux was not found in the fatty acid concentration greater than 5%, indicating the enhancement effect is in a bell-shaped currie. In a study of the effect of AM concentration on the permeation, there was no difference in the permeation rate between 0.5 and 1% for AM, below its saturated concentration. At the donor concentration of 2%, over the saturated condition, the flux of AM was markedly increased. A considerable degradation of AM was found during permeation studies, and the extent was correlated with protein concentrations in the epidermal and serosal extracts, and skin homogenates. In rat dorsal skins, the protein concentration decreased in the rank order of skin homogenate > serosal extract > epidermal extract. Estimated first order degradation rate constants were $6.15{\pm}0.14,{\;}0.57{\pm}0.02{\;}and{\;}0.011{\pm}{\;}0.004{\;}h^{-1}$ for skin homogenate, serosal extract and epidermal extract, respectively. Therefore, it appeared that AM was hydrolyzed to some extent into salicylmaltol by esterases in the dermal and subcutaneous tissues of skin. taken together, our data indicated that transdermal delivery of AM is feasible when the combination of PC and LOA is used as a vehicle. However, since AM is not metabolically stable, acceptable degradation inhibitors may be nervessary to fully realize the transdermal delivery of the drug.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 1999.05a
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• pp.269-274
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• 1999

Aluminum alloy has not only physical characteristic of very high activated high thermal conductivity and high thermal coefficient expansion but also special characteristic of great difference fusibility of hydrogen between liquid and solid phase. Because of these reasons, Aluminum welding is very different. Therefore, only MIG welding method should be applied instead of other welding methods. In this study, in order to select optimal welding conditions, it has been to investigate the effectiveness on the welding current, welding speed, flow rate of gas and welding voltage to occurrence of spatters, external shape of bead, state of penetration and width and hight of bead by using filer metal of A15356(dia. 1.21mm) on the base material of A16061.