[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.9729/AM.2016.46.4.188

How to Get Well-Preserved Samples for Transmission Electron Microscopy

Park, Chang-Hyun (Medical Science Research Center, Korea University College of Medicine)
Kim, Hyun-Wook (Medical Science Research Center, Korea University College of Medicine)
Rhyu, Im Joo (Department of Anatomy, Korea University College of Medicine)
Uhm, Chang-Sub (Department of Anatomy, Korea University College of Medicine)

Publication Information

Applied Microscopy / v.46, no.4, 2016 , pp. 188-192 More about this Journal

Abstract

Proper sample preparation prior to microscopy is necessary for maintaining the components of tissues in a state as close to a living state as possible. For optimal preservation of biological samples, the sampling conditions are as important as the fixation itself. Various factors influence the selection and fixation efficiencies of a fixative, including sample size, osmolarity, pH, penetration rate and depth, fixative temperature, fixation time, fixative concentration, fixative amount, and retention time. Therefore, several factors for selecting and administering fixation procedures are evaluated pertaining to optimal sample preparation for transmission electron microscopy.

Keywords

Fixation; Penetration; Fixative;

Citations & Related Records

Reference

1	Bozzola J J and Russell L D (1992) Electron Microscopy (Jones and Bartlett Publishers, Boston).
2	Hayat M A (1973) Principles and Techniques of Electron Microscopy. Biological Applications (Van Nostrand Reinhold Co., New York, etc.).
3	Hayat M A (1981) Fixation for Electron Microscopy (Academic Press, New York).
4	Lewis P P and Knight D P (1977) Staining Methods for Sectioned Material. In: Practical Methods in Electron Microscopy (North-Holland Publ., Amsterdam).
5	Romert P and Matthiessen M E (1975) Fixation of foetal pig liver for electron microscopy. I. The effect of various aldehydrs and of delayed fixation. Anat. Embryol. 147, 243-258. DOI
6	Saito Y and Tanaka Y (1980) Glutaraldehyde fixation of fish tissues for electron microscopy. J. Electron Microsc. 29, 1-7.
7	Smith R E (1970) Comparative evaluation of 2 instruments and procedures to cut nonfrozen sections. J. Histochem Cytochem. 18, 590-591. DOI
8	Uhm C S, Park E K, and Park C H (1998) Tissue preparation with t-Butyl alcohol freeze-drying method for scanning electron microscopy: application for rat liver. Korean J. Electron Microsc. 28, 299-306.