• Title/Summary/Keyword: Optical microscope

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Determining Two-Sided Surface Profiles of Micro-Optical Elements Using a Dual-Wavelength Digital Holographic Microscope With Liquids

  • Lee, Hong Seok;Shin, Sanghoon;Lee, Heonjoo;Yu, Younghun
    • Journal of the Optical Society of Korea
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    • v.18 no.5
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    • pp.495-499
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    • 2014
  • In this paper, a method is proposed for simultaneously measuring the front and back surface profiles of transparent micro-optical components. The proposed method combines a dual-wavelength digital holographic microscope with liquids to record holograms at different wavelengths, and then numerically reconstructs the three-dimensional phase information to image the front and back sides of the sample. A theoretical model is proposed to determine the surface information, and imaging of an achromatic lens is demonstrated experimentally. Unlike conventional interferometry, our proposed method supports nondestructive measurement and direct observation of both front and back profiles of micro-optical elements.

The Optical Design of Probe-type Microscope Objective for Intravital Laser Scanning CARS Microendoscopy

  • Rim, Cheon-Seog
    • Journal of the Optical Society of Korea
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    • v.14 no.4
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    • pp.431-437
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    • 2010
  • A stack of gradient-index (GRIN) rod lenses cannot be used for coherent anti-Stokes Raman scattering (CARS) microendoscopy for insertion to internal organs through a surgical keyhole with minimal invasiveness. That's because GRIN lens has large amount of inherent chromatic aberrations in spite of absolutely requiring a common focus for pump and Stokes beam with each frequency of ${\omega}_p$ and ${\omega}_S$. For this endoscopic purpose, we need to develop a long slender probe-type objective, namely probe-type microscope objective (PMO). In this paper, we introduce the structure, the working principle, and the design techniques of PMO which is composed of a probe-type lens module (PLM) and an adaptor lens module (ALM). PLM is first designed for a long slender type and ALM is successively designed by using several design parameters from PLM for eliminating optical discords between scanning unit and PLM. A combined module is optimized again to eliminate some coupling disparities between PLM and ALM for the best PMO. As a result, we can obtain a long slender PMO with perfectly diffraction-limited performance for pump beam of 817 nm and Stokes beam of 1064 nm.

Effective Area in CCD and Reconstruction Area in the reflection type Digital Holography microscope system (반사형 디지털 홀로그래피 현미경 시스템에서 촬상소자의 유효기록 가능영역과 재생영역에 대한 연구)

  • Choe, Gyu-Hwan;Kim, Seong-Gyu
    • Proceedings of the Optical Society of Korea Conference
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    • 2008.07a
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    • pp.169-171
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    • 2008
  • 마이켈슨 간섭계 시스템에 두 개의 Convex Lens를 추가함으로서 평면파 참조광을 유지 시키며, 물체에 조사되는 물체광의 조사면적을 변화시킬 수 있는 새로운 형태의 마이켈슨 간섭계를 제작 하였다. 새로운 형태의 마이켈슨 간섭계를 반사형 디지털 홀로그래피 현미경 시스템(Digital Holography Microscope System)에 적용하여 촬상소자(CCD: Charge Couple Device)에 홀로그램 기록 시 기록 가능한 CCD의 유효 영역과 재생 가능 영역에 대해 연구를 하였다

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Measurement of Relative Phase Distribution of Onion Epidermal cells by using Relative Phase Microscope (상대위상 현미경을 이용한 양파 표피세포의 상대위상 측정)

  • Sin, In-Hui;Kim, Deok-Yeong
    • Proceedings of the Optical Society of Korea Conference
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    • 2006.07a
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    • pp.147-148
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    • 2006
  • Relative phase distribution of onion epidermal cells was measured by using the relative phase microscope with inverse linear polarizing method. Decrease of relative phase distribution of onion epidermal cells was also investigated as the elapse of time. In decrease of relative phase distribution, relative phase of cell membrane in onion epidermal cells decreased radically as compared with that of cytoplasm.

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OPTICAL SENSITIVITY OF LASER FLUORESCENCE FOR INCIPIENT CARIES DETECTION (초기우식병소에 대한 레이저 fluorescence의 광학적 탐지감도)

  • Kim, Hyo-Suck;Kim, Wang-Kwen;Lee, Chang-Seop;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.26 no.1
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    • pp.109-118
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    • 1999
  • The aim of this study was to evaluate the optical density of laser fluorescence for detection of incipient caries. Prepared and polished bovine enamel specimens were demineralized in a STPP solution for varying periods of time between 3 hrs. and 60 hrs. with an area of sound enamel retained on each specimen. The randomized specimens were analyzed for optical density of enamel demineralization using laser fluorescence. The specimens were sectioned and examined lesion depth by polarizing light microscope. Results were analyzed statistically with SAS program. The results from this study can be summarized as follows: 1. Optical density measured by laser fluorescence and lesion depth measured by polarizing light microscope was increased as demineralization time was increased(p<0.001). 2. Between optical density measured by laser fluorescence and lesion depth measured by polarizing light microscope was correlated highly(${\gamma}{\geq}0.74956$, p<0.001). 3. Regressive equation was obtained in this study as follows. Y=[X-0.260851]/0.000271(R-square:0.5618, p<0.001) (X:DENSITY, Y:DEPTH) In summary, optical density measured by laser fluorescence would be within the range of possibility to quantitatively presume demineralization amount of incipient caries lesion

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Development of a soft X-ray microscopy system for Biological Application (생물의료용 연 X-선 현미경 시스템 개발)

  • Kim, Gyeong-U;Gwon, Yeong-Man;Kim, Gyu-Gyeom;Min, Jong-Hwan;Park, Jeong-Gwon;Im, Jong-Hyeok;Nam, Gi-Yong;Yun, Gwon-Ha;Min, Jin-Yeong
    • Proceedings of the Optical Society of Korea Conference
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    • 2005.02a
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    • pp.264-265
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    • 2005
  • In this paper the conceptual design and development of a compact vertical type soft x-ray microscope is described. This x-ray microscope operates in the water window wavelength region(2.3 ${\sim}$ 4.4nm), where natural contrast between carbon(protein) and oxygen(water) allows imaging of unstained biological material their natural, hydrated environment. Until now, operational x-ray microscopes are based on synchrotron radiation sources, which limit their accessibility. Many biologists would benefit from having the x-ray microscope as a tool among other tools in their own laboratory, For this purpose we introduced the compact vertical type soft X-ray microscope with 50 nm resolution for biomedical application. The compact vertical type soft x-ray microscope is based on a laser plasma x-ray source, doubled ellipsoidal condenser reflective optics, diffractive zone plate optics and MCP coupled with CCD to record an x-ray image.

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Effects of Electroplating Condition on Micro Bump of Multi-Layer Build-Up PCB (다층 PCB 빌드업 기판용 마이크로 범프 도금에 미치는 전해조건의 영향)

  • Seo, Min-Hye;Hong, Hyun-Seon;Jung, Woon-Suk
    • Korean Journal of Materials Research
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    • v.18 no.3
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    • pp.117-122
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    • 2008
  • Micro-sized bumps on a multi-layered build-up PCB were fabricated by pulse-reverse copper electroplating. The values of the current density and brightener content for the electroplating were optimized for suitable performance with maximum efficiency. The micro-bumps thus electroplated were characterized using a range of analytical tools that included an optical microscope, a scanning electron microscope, an atomic force microscope and a hydraulic bulge tester. The optical microscope and scanning electron microscope analyses results showed that the uniformity of the electroplating was viable in the current density range of $2-4\;A/dm^2$; however, the uniformity was slightly degraded as the current density increased. To study the effect of the brightener concentration, the concentration was varied from zero to 1.2 ml/L. The optimum concentration for micro-bump electroplating was found to be 0.6 ml/L based on an examination of the electroplating properties, including the roughness, yield strength and grain size.