• Title/Summary/Keyword: Optical cell

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Growth of Budding Yeasts under Optical Trap

  • Im, Kang-Bin;Kim, Hyun-Ik;Kim, Soo-Ki;Kim, Chul-Geun;Oh, Cha-Hwan;Song, Seok-Ho;Kim, Pill-Soo
    • Molecular & Cellular Toxicology
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    • v.3 no.1
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    • pp.19-22
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    • 2007
  • Optic tweezer is powerful tool to investigate biologic cells. Of eukaryotic cells, it was poorly documented regarding to optic trapping to manipulate yeasts. In preliminary experiment to explore yeast biology, interferometric optical tweezers was exploited to trap and manipulate budding yeasts. Successfully, several budding yeasts are trapped simultaneously. We found that the budding direction of the daughter cell was almost outward and the daughter cell surrounded by other yeasts grows slowly or fail to grow. Thus it was assumed that neighboring cells around budding yeast may be critical in budding and the growth of daughter cells. This is first report pertaining to the pattern of yeast budding under the optical trap when multiple yeasts were trapped.

Study on Analysis of Optical Deflection of Laser Scattering Based on Rayleigh Criterion for Crystalline Silicon Wafer in Solar Cell (태양전지용 결정질 실리콘 웨이퍼에서의 레일리기준 기반 레이저산란의 광편향 분석에 관한 연구)

  • Kim, Gyung-Bum
    • Journal of the Semiconductor & Display Technology
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    • v.9 no.4
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    • pp.31-37
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    • 2010
  • In this paper, optical deflection of laser scattering has been investigated based on Rayleigh criterion for crystalline silicon wafer in solar cell. A laser scattering mechanism is newly designed using light scattering properties in silicon wafer. Intensity distributions of laser scattering are different, depending on the incident angle of laser computed from Rayleigh criterion. In case of the incident angle satisfied with the criterion, they are asymmetric. Also, their specular reflection angle is shifted to unpredicted ones. These phenomena are in accordance with previous theories of laser scattering. The optical deflection of laser scattering is experimentally identified with the designed laser scattering mechanism. Its mathematical model is presented from the geometric relationship of laser scattering. It is shown that the optical deflection of laser scattering agree with the presented model, exclusive of grazing angles which is satisfied with Rayleigh criterion.

Electro-optical performance using a PDT-VA cell (PDT-VA 셀을 이용한 전기광학 특성)

  • 김형규;황정연;서대식;한은주;김재형
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2000.11a
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    • pp.133-136
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    • 2000
  • We investigated the improvement of viewing angle using a patterned double twisted (PDT) vertical-alignment (VA) cell mode on a homeotropic alignment layer. Good voltage-transmittance curves for negative dielectric anisotropic nematic liquid crystal (NLC) using the PDT-VA cell without a negative compensation film were obtained. The viewing angle of the PDT-VA cell without a negative compensation film was wider than that of a conventional VA cell.

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Electro-optical performance using a PDT-VA cell (PDT-VA 셀을 이용한 전기광학 특성)

  • 김형규;황정연;서대식;한은주;김재형
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2000.11a
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    • pp.133-136
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    • 2000
  • We investigated the improvement of viewing angle using a patterned double twisted (PDT) vertical-alignment (VA) cell mode on a homeotropic alignment layer. Good voltage-transmittance curves for negative dielectric anisotropic nematic liquid crystal (NLC) using the PDT-VA cell without a negative compensation film were obtained. The viewing angle of the PDT-VA cell without a negative compensation film was wider than that of a conventional VA cell.

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EFFECT OF TITANIUM SURFACE ROUGHNESS ON CELL ADHESION OF HUMAN OSTEOBLAST-LIKE CELLS (MG63)

  • Yim Soon-Ho
    • The Journal of Korean Academy of Prosthodontics
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    • v.42 no.3
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    • pp.261-266
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    • 2004
  • Statement of problem. The effects of surface roughness have not or insufficiently been analyzed on earlier events such as cell adhesion though cell behavior most germane to implant performance is cell adhesion. Purpose. The purpose of this study was to evaluate cell adhesion of osteoblast-like cells (MG63) onto three types of titanium disks with varying roughness using the Elisa assay. Materials and methods. Representative disks from each group (SLA, HA, machined) were subjected to surface analysis and surface roughness was measured by the optical interferometer (Accura 2000, Intekplus Co., Seoul, Korea). Following this, MG63 cells were cultured on the titanium disks and released. Cell adhesion measurements using the Elisa assay were performed specifically at three points: after 24, 48, and 72 hours of culture. Results. Among the 3 types of surface analyzed, the SLA surface was the roughest with a Ra value of $1.114{\mu}m$ followed by HA coated surface and machined surface, consecutively. The optical density values for the SLA surface group was significantly higher than that of the machined and HA coated surface groups following 24 and 48 hours of culture. The cell culture on HA coated surface showed significantly higher values compared to the machined surface following 24, 48 and 72 hours of culture. Conclusion. The results suggest that surface treatment of titanium surfaces enhanced cell adhesion of human osteoblast-like cells (MG63).

An Optical Configuration for Vertical Alignment Liquid Crystal cell with Wide Viewing Angle

  • Ji, Seung-Hoon;Lee, Gi-Dong
    • Journal of Information Display
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    • v.9 no.2
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    • pp.22-27
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    • 2008
  • We propose an optical configuration of a vertical alignment (VA) liquid crystal (LC) cell to eliminate the light leakage in the diagonal direction. VA LC cell has an excellent contrast ratio in the normal direction due to the no phase-retardation. However, change of the phase-retardation occurs in all directions, which causes the light leakage and deteriorates the characteristics of the dark state. We designed the LC cell structure composed of multiple combinations with two A-plates and two C-plates in order to achieve wide viewing property on the Poincare sphere. From calculations, we show that the proposed structure can improve the viewing angle characteristics by compensating for the light leakage in all directions.

Electro-Optical Performances of In plane Switching(IPS) Cell on the Inorganic Thin Film by DuoPIGatron Ion Source (NDLC박막에 DuoPIGatron 이온소스를 사용한 IPS cell의 전기광학특성)

  • Kim, Sang-Hoon;Kim, Jong-Hwan;Kang, Dong-Hoon;Kim, Young-Hwan;Hwang, Jeoung-Yeon;Seo, Dae-Shik
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.06a
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    • pp.453-454
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    • 2006
  • We studied the nematic liquid crystal (NLC) alignment capability by the IB(Ion bean) alignment method on a NDLC(Nitrogen Diamond Like Carbon) as a-C:H thin film. and investigated electro-optical performances of the IBaligned IPS(In plane switching)cell with NDLC surface. A good LC alignment by IB exposure on a NDLC surface was achieved. Monodomain alignment of the IB aligned IPS cell can be observed. The goodelectro-optical (EO) characteristics of the IB aligned IPS cell was observed with oblique IBexposure on the NDLC as a-C:H thin film for 1 min.

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A Spatial-domain Fourier Transform Infrared Spectrometer: Application for Analyte Measurement in Cell Culture Media

  • Jung, Byung-Jo
    • Journal of the Optical Society of Korea
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    • v.9 no.4
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    • pp.151-156
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    • 2005
  • A spatial-domain Fourier Transform (FT) infrared (IR) spectrometer coupled with a PtSi Schottky­barrier IR detector plane was developed in the spectral range of $2.0-2.5{\mu}m$ for noninvasive measurement of analyte concentrations in cell culture media during cell culture processing. A key optical component of the spectrometer is a Savart plate which is a birefringent polarizer generating coherent two rays for interfering. The spectral resolution of the spectrometer was determined as $71cm^{-1}$ (${\~}0.05{\mu}m$ at $2.5{\mu}m$). Clear IR fringe patterns were imaged on the IR detector plane. The feasibility of the spectrometer for our application was investigated by measuring absorbance spectra of glucose and fetal bovine serum (FBS) which are important compounds in cell culture media. Experiment results show that the spectral quality of glucose and FBS was comparable with the standard spectra acquired with a commercial FT-IR spectrometer, presenting the feasibility of the spectrometer to perform analyte measurement in cell culture media.