• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2002.05a
• /
• pp.490-493
• /
• 2002

Many servers that is operated in present earth are UNIX base, is trend that server of LINUX base is increasing steadily recently. When users who have account to this server wish to do remote access, instruction that use most easily is‘telnet’, security does not consist entirely about ID and password that this uses at communication substance as well as login. The interest about latest SSH is rising by the alternative, but SSH has various kinds problem in following telnet's fame. Therefore, We studied about problems and the solution that can happen when window users attempted remote access laying stress on OpenSSH.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2015.05a
• /
• pp.636-638
• /
• 2015

Take advantage of smart devices is time and place constraints beyond the Office, home, school and a Korean or foreign travel was being keep doing it yourself anywhere. But in order to build the infrastructure of these network-to-network VPN-only expert support and expensive equipment is needed for some reason does not go as far as small-to medium-sized institutions, popularised. In this paper, H/W wired/wireless router a cheap, open platforms, open network-only embedded OS is based on OpenSSH, OpenVPN, OpenWRT platforms to implement network VPN iproute support system design and implementation.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.19 no.5
• /
• pp.1097-1103
• /
• 2015

In order to build network infrastructure to implement the aforementioned advantages enabling smart device users to work anywhere, professional support and expensive VPN devices are required. This is a barrier to supplying VPN devices to small and medium-sized institutes. To address this issue, this study aims to implement OpenVPN, OpenSSH and iproute based on the OpenWRT platform which is an embedded OS for open networks in affordable open wired/wireless gateway H/W platforms to support the inter-network VPN. In addition, the network environment can be maintained optimal by applying a "multi-queuing real-time traffic shaping technology" to VPN tunnels, although channel quality changes.

• Journal of Plant Biotechnology
• /
• v.29 no.2
• /
• pp.79-84
• /
• 2002

Suppression subtractive hybridization (SSH) was used to isolate wound-induced cDNAs from wounded soybean. One of wound-induced cDNA, gmwi33 showed high homology with genes encoding $\beta$-amyrin synthase. The full length cDNA of gmwi33, designated GmAMS1, is 2416 bp long and contains an open reading frame consisted of 739 amino acids. GmAMS1 protein showed 89% identity with licorice GgbAS1 and 86% identity with pea OSCPSY. In 5 day-old, dark-grown seedlings, the expression of GmAMS1 was most strongly induced by light and weakly induced by methyl jasmonate and by low temperature. However, GmAMS1 was not induced by elicitor or UV-B treatment. Such expression pattern might be closely related with the oxygen-radical scavenging activity of soyasaponin.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.69.1-69
• /
• 2003

RSH (relA/spoT homolog) has been known to determine the level of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), which are the effector nucleotide of the prokaryotic stringent response and also play a role in antibiotic production and differentiation in Streptomyces species but not a little in eukaryotic organism, especially in plant. Salicylic acid (SA), a critical signal molecule of establishing systemic acquired resistance (SAR), could induce SAR in Pepper (Capcicum annuum) against Phytophthora capsici. And the extent of SAR induction was in proportion to the dosage of SA (or BTH). Suppression subtractive hybridization (SSH), a PCR-based method for cDNA subtraction, was carried out between SA-treated and non-SA-treated pepper leaves to isolate genes which may be responsible for defense signaling against pathogens. Early upregulated gene was selected from reverse northern and kinetics of SSH-genes transcripts in SA-treated pepper leaves upon SA treatment. Full-length cDNA of the gene (PepRSH； Pepper RelA / SpoT homolog) had an open reading frame (ORF) of 2166 bp encoding a protein of 722 amino acids and a significant homology with (p)ppGpp phosphohydrolase or synthetase. Genomic DNA gel blot analysis showed that pepper genome has at least single copy of PepRSH. PepRSH transcripts was very low in untreated pepper leaves but strongly induced by SA and methyljasmonic acid (MeJA), indicating that PepRSH may share common SA and MeJA-mediated signal transduction pathway Functional analysis in E. coli showed PepRSH confers phenotypes associated with (p)ppGpp synthesis through a complementation using active site mutagenesis.

• Journal of Plant Biotechnology
• /
• v.29 no.2
• /
• pp.99-104
• /
• 2002

Suppression subtractive hybridization (SSH) was used to isolate wound-induced cDNAs from wounded soybean. One of low-temperature-inducible cDNA, slti182 showed high homology with genes encoding 1-asparaginase. The full length cDNA of slti182, deginated GmASP1, is 1258 bp long and contains an open reading frame consisted of 326 amino acids. CmASP1 protein showed the highest identity (84%) with putative asparaginase from A. thaliana (AB012247), but it showed only 55% identity with another isoform of A. tathaliana (Z34884). The expression of GmASP1 during low temperature stress started to increase 3 hours after treatment, reached the maximum at 6 hour, and then decreased to the initial level at 48 hours. The amount of GmASP1 transcripts increased again when low-temperature-treated plants were transferred to room temperature, The present study suggests that GmASP1 may function to accelerate the protein synthesis which is important in the early response to low temperature.