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http://dx.doi.org/10.5010/JPB.2002.29.2.079

Molecular Cloning and Characterization of Wound-inducible Beta-amyrin Synthase from Soybean  

Park, Seong-Whan (Faculty of Natural Resources and Life science, Dong-A university)
Lee, Jai-Heon (Faculty of Natural Resources and Life science, Dong-A university)
Publication Information
Journal of Plant Biotechnology / v.29, no.2, 2002 , pp. 79-84 More about this Journal
Abstract
Suppression subtractive hybridization (SSH) was used to isolate wound-induced cDNAs from wounded soybean. One of wound-induced cDNA, gmwi33 showed high homology with genes encoding $\beta$-amyrin synthase. The full length cDNA of gmwi33, designated GmAMS1, is 2416 bp long and contains an open reading frame consisted of 739 amino acids. GmAMS1 protein showed 89% identity with licorice GgbAS1 and 86% identity with pea OSCPSY. In 5 day-old, dark-grown seedlings, the expression of GmAMS1 was most strongly induced by light and weakly induced by methyl jasmonate and by low temperature. However, GmAMS1 was not induced by elicitor or UV-B treatment. Such expression pattern might be closely related with the oxygen-radical scavenging activity of soyasaponin.
Keywords
Light-inducible; saponins; suppression subtractive hybridization; triterpene biosynthesis;
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