• Mycobiology
• /
• v.42 no.2
• /
• pp.167-173
• /
• 2014

A ${\beta}$-glucan synthase gene was isolated from the genomic DNA of polypore mushroom Sparassis crispa, which reportedly produces unusually high amount of soluble ${\beta}$-1,3-glucan (${\beta}$-glucan). Sequencing and subsequent open reading frame analysis of the isolated gene revealed that the gene (5,502 bp) consisted of 10 exons separated by nine introns. The predicted mRNA encoded a ${\beta}$-glucan synthase protein, consisting of 1,576 amino acid residues. Comparison of the predicted protein sequence with multiple fungal ${\beta}$-glucan synthases estimated that the isolated gene contained a complete N-terminus but was lacking approximately 70 amino acid residues in the C-terminus. Fungal ${\beta}$-glucan synthases are integral membrane proteins, containing the two catalytic and two transmembrane domains. The lacking C-terminal part of S. crispa ${\beta}$-glucan synthase was estimated to include catalytically insignificant transmembrane ${\alpha}$-helices and loops. Sequence analysis of 101 fungal ${\beta}$-glucan synthases, obtained from public databases, revealed that the ${\beta}$-glucan synthases with various fungal origins were categorized into corresponding fungal groups in the classification system. Interestingly, mushrooms belonging to the class Agaricomycetes were found to contain two distinct types (Type I and II) of ${\beta}$-glucan synthases with the type-specific sequence signatures in the loop regions. S. crispa ${\beta}$-glucan synthase in this study belonged to Type II family, meaning Type I ${\beta}$-glucan synthase is expected to be discovered in S. crispa. The high productivity of soluble ${\beta}$-glucan was not explained but detailed biochemical studies on the catalytic loop domain in the S. crispa ${\beta}$-glucan synthase will provide better explanations.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.42 no.3
• /
• pp.237-242
• /
• 2014

Surge phenomenon can be occurred in a compressor when the performance of turbofan engine for an aircraft is changed considerably such as take-off phase. This study is aimed to avoid surge phenomenon. This paper propose the PID and Fuzzy control System for the turbofan engine with control inputs, the VIGV(Variable Inlet Guide Vane) in closed loop, and the fuel mass flow in open loop. We design the Dynamic modeling, NPSS S-function, which is connection block of simulink between NPSS(Engine analysis program) and Simulink. Finally, we certify the performance to prevent a serge phenomenon in the VIGV control system using the both methods, PID and fuzzy.

• Journal of Bio-Environment Control
• /
• v.5 no.1
• /
• pp.57-64
• /
• 1996

To use effectively the solar energy in greenhouse heating, a high performance solar collector should be developed. And then the size of the solar collector and thermal storage tank should be determined through the calculation of heating load. The solar collector must be set in the optimum tilt angle and direction to take daily solar radiation maximally, and the flow rate of heat transfer fluid through the solar collector should be kept in the optimum range. In this research, the performance tests of a capillary tube solar collector were performed to determine the optimum water flow rate and the results summarized as follows. 1. The regressive equations for efficiency estimations of the capillary tube solar collector in the open loop were modeled in the water flow rate of 700-l,000 $\ell$/hr. 2. The optimum water flow rate of the solar collector was estimated by the second order polynomial regression and the maximum efficiency was 80% at the water flow rate of 850 $\ell$/hr. 3. The solar thermal storage system consisted of a capillary tube solar collector and a water storage tank was tested at the water flow rate of 850 $\ell$/hr in the closed loop, and obtained the solar thermal storage efficiency of 55.2%. 4. As the capillary tube solar collector engaged in this experiment was made of non-corrosive polyolefin tubes, its weight was as light as 1/30 of the flat plate solar collector made of copper tubes. Therefore it was considered to be suitable for the greenhouse heating system.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.29 no.4 s.235
• /
• pp.534-539
• /
• 2005

Nodal displacements are referred to the initial configuration in the total Lagrangian formulation and to the last converged configuration in the updated Lagrangian furmulation. This research proposes a relative nodal displacement method to represent the position and orientation for a node in truss structures. Since the proposed method measures the relative nodal displacements relative to its adjacent nodal reference frame, they are still small for a truss structure undergoing large deformations for the small size elements. As a consequence, element formulations developed under the small deformation assumption are still valid for structures undergoing large deformations, which significantly simplifies the equations of equilibrium. A structural system is represented by a graph to systematically develop the governing equations of equilibrium for general systems. A node and an element are represented by a node and an edge in graph representation, respectively. Closed loops are opened to form a spanning tree by cutting edges. Two computational sequences are defined in the graph representation. One is the forward path sequence that is used to recover the Cartesian nodal displacements from relative nodal displacement sand traverses a graph from the base node towards the terminal nodes. The other is the backward path sequence that is used to recover the nodal forces in the relative coordinate system from the known nodal forces in the absolute coordinate system and traverses from the terminal nodes towards the base node. One open loop and one closed loop structure undergoing large deformations are analyzed to demonstrate the efficiency and validity of the proposed method.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.33 no.6
• /
• pp.565-571
• /
• 2000

Mixed primers based on the high sequence homology of selected regions of known connexins (Cxs) was used for PCR reaction. A full-length connexin cDNA of sweetfish (Plecoglossus altivelis) was cloned by rapid amplification of cDNA 5'and (5'RACE) and 3'RACE method. When compared to other known Cx sequences, homology of sweetfish Cx cDNA to Atlantic croaker, Mycropogonias undulatus Cx32.7, bovine, Bos taurus Cx44 and Atlantic croaker Cx32.2 were $63.8{\%},\;61.6{\%}\;and\;56.7{\%}$, respectively. This cDNA encoded 308 amino acids (35,028 dalton) and named as sweetfish Cx35. Hydropathicity analysis of predicted amino acid sequences indicated that sweetfish Cx35 have four major hydrophobic regions and four major hydrophilic regions, suggesting its topology is similar to that of known Cxs. The presence of a tfical Cx consensus sequences were identified in each of the extracellular loops (first loop and second loop).

• Journal of Power Electronics
• /
• v.16 no.5
• /
• pp.1904-1917
• /
• 2016

The moving average filter (MAF) is widely utilized to improve the disturbance rejection capability of phase-locked loops (PLLs). This is of vital significance for the grid-integration and stable operation of power electronic converters to electric power systems. However, the open-loop bandwidth is drastically reduced after incorporating a MAF into the PLL structure, which makes the dynamic response sluggish. To overcome this shortcoming, some new techniques have recently been proposed to improve the transient response of MAF-based PLLs. In this paper, a comprehensive performance comparison of advanced MAF-based PLL algorithms is presented. This comparison includes HPLL, MPLC-PLL, QT1-PLL, and DMAF-PLL. Various disturbances, such as grid voltage sag, voltage flicker, harmonics distortion, phase-angle and frequency jumps, DC offsets and noise, are considered to experimentally test the dynamic performances of these PLL algorithms. Finally, an improved positive sequence extraction method for a HPLL under the frequency jumps scenario is presented to compensate for the steady-state error caused by non-frequency adaptive DSC, and a satisfactory performance has been achieved.

• KIEAE Journal
• /
• v.17 no.3
• /
• pp.119-124
• /
• 2017

Purpose: Groundwater heat pump (GWHP) system has high coefficient of performance than conventional air-source heat pump system and closed-loop type geothermal system. However, there is problem in long-term operation that groundwater raise at the diffusion well and reduced at the supply well. Therefore, it is necessary to accurately predict the groundwater flow, groundwater movement and control the groundwater level in the wells. In this research, in consideration of hydrogeological characteristic, groundwater level and groundwater movement were conducted analysis in order to develop the optimal design method of the two-well system using the pairing pipe. Method: For the optimum design of the two-well system, this research focused on the design method of the pairing pipe in the simulation model. Especially, in order to control the groundwater level in wells, pairing pipe between the supply well and diffusion well was developed and the groundwater level during the system operation was analyzed by the numerical simulation. Result: As the result of simulation, the groundwater level increased to -2.65m even in the condition of low hydraulic conductivity and high pumping flow rate. Consequently, it was found that the developed system can be operated stably.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.31 no.3
• /
• pp.39-49
• /
• 2003

The delta operator approach and the singular perturbation technique are introduced. The former reduces the round-off error in the numerical computation. The latter reduces computing time by decoupling the original system into the fast and slow sub-systems. The aircraft dynamics consists of the Phugoid and short-period motions whether its model is longitudinal or lateral. In this paper, an approximated solutions of lateral dynamic model of Beaver obtained by using those two methods in compared with the exact solution. For open-loop system and closed-loop system, and approximated solution gets identical to the exact solution with only one iteration and without iteration, respectively. Therefore, it is shown that implementing those approaches is very effective in the flight dynamic and control.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.21 no.6
• /
• pp.347-354
• /
• 2009

Experimental study on a cross-flow air-cooled plate heat exchanger (PHE) was performed. Two types of PHEs were manufactured either with single-wave plates or with double-wave plates in parallel. Cooling air flows through the PHEs in a crosswise direction against internal hot water. The heat exchanger aims to substitute open-loop cooling towers with closed-loop water circulation, which guarantees cleanliness and compactness. In this study, prototype single-wave and double-wave PHEs were designed and tested in a laboratory scale experiments. From the tests, the double-wave PHE shows approximately 50% enhanced heat transfer performance compared to the single-wave PHE. However, the double-wave PHE costs 30% additional pressure drop. For the commercialization, a wide channel design for air flow would be essential for performance and reliability.

• Molecules and Cells
• /
• v.26 no.1
• /
• pp.26-33
• /
• 2008

The plasmid pJB01, a member of the pMV158 family isolated from Enterococcus faecium JC1, contains three open reading frames, copA, repB, and repC. Plasmids included in this family produce counter-transcribed RNA (ctRNA) that contributes to copy number control. The pJB01 ctRNA, a transcript which consists of 54 nucleotides (nts), is encoded on the opposite strand from the copA/repB intergenic region and partially overlaps an atypical ribosome binding site (ARBS) for repB. The ARBS is integrated by the two underlined conserved regions: 5'-TTTTTGTNNNNTAANNNNNNNNNATG-3', and the ctRNA is complementary only to the 5' conserved sequence 5'-TTTTTGT-3'. This complementary sequence is located at a distance from the terminal loop of the ctRNA secondary structure. The ctRNA structure predicted by the mfold program suggests the possible generation of a terminal and an internal hairpin loop. The amount of in vitro translation product of repB mRNA was inversely proportional to the ctRNA concentration. Mutations in the terminal and internal hairpin loops of the ctRNA had inhibitory effects on its binding to the target mRNA. We propose that the intact structures of the terminal and internal hairpin loops, respectively, play important roles in forming the initial kissing and extending complexes between the ctRNA and target mRNA and that these regulate the copy number of this plasmid.