• Natural Product Sciences
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• v.21 no.2
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• pp.111-121
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• 2015

The root of Panax ginseng, is a Korea traditional medicine, which is used in both raw and processed forms due to their different pharmacological activities. As part of a continued chemical investigation of ginseng, the focus of this research is on the isolation and identification of compounds from Panax ginseng root by open column chromatography, medium pressure liquid chromatography, semi-preparative-high performance liquid chromatography, Fast atom bombardment mass spectrometric, and nuclear magnetic resonance. Dammarane-type triterpenoid saponins were isolated from Panax ginseng root by open column chromatography, medium pressure liquid chromatography, and semi-preparative-high performance liquid chromatography. Their structures were identified as protopanaxadiol ginsenosides [gypenoside-V (1), ginsenosides-Rb1 (2), -Rb2 (3), -Rb3 (4), -Rc (5), and -Rd (6)], protopanaxatriol ginsenosides [20(S)-notoginsenoside-R2 (7), notoginsenoside-Rt (8), 20(S)-O-glucoginsenoside-Rf (9), 6-O-[$\alpha$-L-rhamnopyranosyl(1$\rightarrow$2-$\beta$-D-glucopyranosyl]-20-O-$\beta$-D-glucopyranosyl-$3\beta$,$12\beta$, 20(S)-dihydroxy-dammar-25-en-24-one (10), majoroside-F6 (11), pseudoginsenoside-Rt3 (12), ginsenosides-Re (13), -Re5 (14), -Rf (15), -Rg1 (16), -Rg2 (17), and -Rh1 (18), and vinaginsenoside-R15 (19)], and oleanene ginsenosides [calenduloside-B (20) and ginsenoside-Ro (21)] through the interpretation of spectroscopic analysis. The configuration of the sugar linkages in each saponin was established on the basic of chemical and spectroscopic data. Among them, compounds 1, 8, 10, 11, 12, 19, and 20 were isolated for the first time from P. ginseng root.

• Korean Journal of Medicinal Crop Science
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• v.8 no.3
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• pp.274-282
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• 2000

Physico-chemical characteristics and chemical composition of seed oil of Sapium japonicum were determined by various analysis methods. Physio-chemical characteristics of the extracted oil from fresh seed were specific gravity (0.928), refraction index (1.477), acid value (2.30), saponification number (190.0), and iodine value (126.0). Neutral lipid (93%) in seed oil was identified as major components, followed by glycolipid (4.9%) and phospholipid (1.3%). In GC analysis, nine fatty acids were presented in the seed oil. Among fatty acids, predominant fatty acids were oleic acid (45.8%) and linoleic acid (35.6%). The contents of fatty acids of seed oil somewhat varied with their storage period. Composition of fatty acids from lipid fractions which were isolated on silica open column was investigated. Major fatty acids in three lipid fractions were linoleic acid and linolenic acid. The fatty acid contents of fresh seed oil derived from each lipid fraction were generally high comapred to storage seed oil. From the above results, seed oil of Sapium Japonicum could be useful in cosmetics, detergents and a few pharmaceuticals.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.29 no.5
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• pp.429-436
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• 2016

In this study, the genetic algorithm based optimal structural design method is proposed. The objective functions are to minimize the cost and $CO_2$ emissions, simultaneously. The cost and $CO_2$ emissions are calculated based on the cross-sectional dimensions, length, material strength, and reinforcement ratio of beam and column members. Thus, the cost and $CO_2$ emissions are evaluated by using the amounts of concrete and reinforcement used to construct a building. In this study, the cost and $CO_2$ emissions calculated at the phases of material transportation, construction, and building operation are excluded. The constraint conditions on the strength of beam and column members and the inter-story drift ratio are considered. The linear static analysis by using OpenSees is automatically conducted in the proposed method. The genetic algorithm is employed to solve the formulated problem. The proposed method is validated by applying it to the 4-story reinforced concrete moment frame example.

• Journal of Ocean Engineering and Technology
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• v.26 no.6
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• pp.53-58
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• 2012

An experimental study on the hydrodynamic performance of a backward bent duct buoy (BBDB) was performed in a 2D wave tank. The BBDB is one of the promising oscillating water column (OWC) types of floating wave energy converters. Two different corner-shaped BBDBs (sharp-corner and round-corner) were used to measure the maximum chamber surface elevations and body motions for various incident wave conditions, and their hydrodynamic characteristics were compared. In order to investigate the effect of the pneumatic pressure inside the chamber, the heave and pitch angle interacted with elevations were compared for both open chamber and partially open chamber BBDBs. From the comparison study, the deviation in the chamber surface elevations between the two shapes of BBDBs was found to be significant near the resonance period, which may be explained by viscous energy loss. It was also found that the pneumatic pressure noticeably affected the chamber surface elevation and body motions.

• Structural Engineering and Mechanics
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• v.76 no.3
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• pp.421-433
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• 2020

Masonry infills are normally considered as non-structural elements in design practice, therefore, the interaction between the bounding frame and the strength contribution of masonry infills is commonly ignored in the seismic analysis work of the RC frames. However, a number of typical RC frames with irregular distributed masonry infills have suffered from undesirable weak-story failure in major earthquakes, which indicates that ignoring the influence of masonry infills may cause great seismic collapse risk of RC frames. This paper presented the investigation on the risk of seismic collapse of RC frames with irregularly distributed masonry infills through a large number of nonlinear time history analyses (NTHAs). Based on the results of NTHAs, seismic fragility curves were developed for RC frames with various distribution patterns of masonry infills. It was found that the existence of masonry infills generally reduces the collapse risk of the RC frames under both frequent happened and very strong earthquakes, however, the severe irregular distribution of masonry infills, such as open ground story scenario, results in great risk of forming a weak story failure. The strong-column weak-beam (SCWB) ratio has been widely adopted in major seismic design codes to control the potential of weak story failures, where a SCWB ratio value about 1.2 is generally accepted as the lower limit. In this study, the effect of SCWB ratio on inter-story drift distribution was also parametrically investigated. It showed that improving the SCWB ratio of the RC frames with irregularly distributed masonry infills can reduce inter-story drift concentration index under earthquakes, therefore, prevent weak story failures. To achieve the same drift concentration index limit of the bare RC frame with SCWB ratio of about 1.2, which is specified in ACI318-14, the SCWB ratio of masonry-infilled RC frames should be no less than 1.5. For the open ground story scenario, this value can be as high as 1.8.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.151-157
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• 2000

This study was carried out to isolate and identify the maysin and related flavonoid analogues in corn silks. Silks were covered with silk bag to prevent pollination and were sampled at 3-5 days after silking. The silks were filled with 100% MeOH and stored at $0^{\circ}C$ until analysis. The MeOH extracts of corn silks were filtered and concentrated at 35-4$0^{\circ}C$. The ${CH}_2$${Cl}_2$ was added on the concentrated aqueous solution to remove the chlorophyll and lipids. The Cis open column (25mm$\times$54 cm) was washed and activated with serial treatment of 500$m\ell$ of 100% MeOH(twice)longrightarrow75% MeOH longrightarrow50% MeOHlongrightarrow30% MeOHlongrightarrow100% $H_2$O(2 times). The concentrated aqueous solution was applied to the $C_{18}$ column and washed with $H_2O$ several times to remove the sugars and water soluble pigments. Neochlorogenic acid, chlorogenic acid and 4-caffeoylquinic acid were eluted with 10% MeOH, and rhamosyl isoorientin was eluted with 30% MeOH, but maysin was eluted with 50% MeOH from the $C_18$ open column. Collected fractions were analyzed with HPLC by using revers-phase Ultras-phere $C_{18}$ column (4.6$\times$250mm, 5$\mu\textrm{m}$) and $H_2$O (10% MeOH containing 0.1% $H_3$${PO}_4$)/MeOH (100% MeOH containing 0.1% H$_3$PO$_4$) linear gradient from 20% to 90% MeOH for 35 minutes, a flow rate of 1 $m\ell$/min and detection at 340nm. The selected fractions were concentrated and applied to the silicic acid column. Maysin was eluted with 500$m\ell$ of 100% ethyl acetate from the silicic acid column for the first purification, and the purity of collected fractions was about 75%, but the purity from the second purification with the Cis column (1/2 $\times$ 43") was greater than 95%. FAB-MS spectral data was obtained with VG7O-VSEQ VG analytical fast atom bombardment mass (UK). $^1$H-NMR and $^{13}$ C-NMR data were obtained with Bruker DPX 400 MHz NMR spectrometers (German) in DMSO-d$_{6}$ at 400 and 100 MHz, respectively.vely.

• Steel and Composite Structures
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• v.22 no.4
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• pp.797-820
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• 2016

This paper simulates the hysteretic behavior of steel reinforced recycled concrete (SRRC) columns under cyclic loads using OpenSees software. The effective fiber model and displacement-based beam-column element in OpenSees is applied to each SRRC columns. The Concrete01 material model for recycled aggregate concrete (RAC) and Steel02 material model is proposed to perform the numerical simulation of columns. The constitutive models of RAC, profile steel and rebars in columns were assigned to each fiber element. Based on the modelling method, the analytical models of SRRC columns are established. It shows that the calculated hysteresis loops of most SRRC columns agree well with the test curves. In addition, the parameter studies (i.e., strength grade of RAC, stirrups strength, steel strength and steel ratio) on seismic performance of SRRC columns were also investigated in detail by OpenSees. The calculation results of parameter analysis show that SRRC columns suffered from flexural failure has good seismic performance through the reasonable design. The ductility and bearing capacity of columns increases as the increasing magnitude of steel strength, steel ratio and stirrups strength. Although the bearing capacity of columns increases as the strength grade of RAC increases, the ductility and energy dissipation capacity decreases gradually. Based on the test and numerical results, the flexural failure mechanism of SRRC columns were analysed in detail. The computing theories of the normal section of bearing capacity for the eccentrically loaded columns were adopted to calculate the nominal bending strength of SRRC columns subjected to vertical axial force under lateral cyclic loads. The calculation formulas of horizontal bearing capacity for SRRC columns were proposed based on their nominal bending strength.

• Structural Engineering and Mechanics
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• v.73 no.3
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• pp.303-317
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• 2020

In this study an innovative rocking zipper braced frame (RZBF) is proposed to overcome the deficiencies of common concentrically braced frames. RZBF is an improved rocking concentrically braced frame which is based on combination of rocking behavior and zipper columns. The base rocking joints and post-tensioned bars provide rocking response and restoring force, respectively. Also, zipper columns distribute the unbalance force over the frame height and reduce the damage concentration. To evaluate seismic performance of RZBF, a comparison study is carried out considering concentrically braced frame, zipper braced frame, rocking concentrically braced frame and RZBF. Thereby, a suite of non-linear time history analyses had been performed on four different types of archetypes with four, six, eight, ten and twelve stories. Frames were designed and non-linear time history analyses were conducted in OpenSees. To compare the seismic behavior of the archetypes, roof drifts, residual roof drifts, story drifts, the forces of first and top story braces, PT bars forces, column uplift and base shears were taken in to consideration. Results illustrate that using RZBF, can reduce the damage due to reduced residual drifts. Zipper columns enhance the seismic performance of rocking systems. As the number of stories increase in the RZBF systems, larger top story braces were needed. So the RZBF system is applicable on low and midrise buildings.

• Structural Engineering and Mechanics
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• v.73 no.2
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• pp.153-165
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• 2020

The effect of soil foundation plays active role in optimum design of steel space frames when included. However, its influence on design can be calculated after a long iterative procedure. So it requires longer computer time and more computational effort if it is done properly. The main purpose of this study is to investigate how these effects can be calculated in more practical way in a shorter time. The effects of semi-rigid column bases are taken into account in optimum design of steel space frames. This study is carried out by using JAYA algorithm which is a novel and practical method based on a single revision equation. The displacement, stress and geometric size constraints are considered in the optimum design. A computer program is coded in MATLAB to achieve corporation with SAP2000-OAPI (Open Application Programming Interface) for optimum solutions. Four different steel space frames including soil structure interaction taken from literature are investigated according to different semi-rigidly supported models depending on different rotational stiffness values. And the results obtained from analyses are compared with the results available in reference studies. The results of the study show that semi-rigidly supported systems in the range of appropriate rotational stiffness values offer practical solutions in a very short time. And close agreement is obtained with the studies on optimum design of steel space frames including soil effect underneath.

• Journal of Environmental Health Sciences
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• v.31 no.3
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• pp.221-226
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• 2005

Isoflavone was extracted with various concentration of aqueous methanol using whole hypocotyls as the starting material. Whole hypocotyls were preferred as the raw material because the residue could be easily removed from the solvent after the extraction process. Extraction yield was almost constant at the methanol concentration of $20-80\%$. Most of the isoflavone was extracted within 1 hr, and the extraction yield remained almost constant thereafter. When the concentration of methanol was $80\%$, the content of total solid was reduced due to the reduced extraction of contaminating protein as the result of protein insolubilization. Among resins tested, Diaion HP-20, Amberlite XAD-16, and Amberlite IRC-50 showed the highest capacity to absorb the compound. Open column chromatography with Diaion HP-20 showed that $80\%$ aqueous ethanol was most efficient as the eluting solvent with final recovery of the phytochemical being more than $95\%$. Maximum adsorption of the phytochemical occurred at the acidic pH 2-4. When the spatial velocity was increased to 15 and more, the degree of adsorption was decreased, whereas below spatial velocity of 15, the adsorption capacity of isoflavone to the resin was almost constant. The purity of the isoflavone purified by column chromatography was $78\%$.