• Title/Summary/Keyword: One-pot

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Siderophore-producing rhizobacteria reduce heavy metal-induced oxidative stress in Panax ginseng Meyer

  • Huo, Yue;Kang, Jong Pyo;Ahn, Jong Chan;Kim, Yeon Ju;Piao, Chun Hong;Yang, Dong Uk;Yang, Deok Chun
    • Journal of Ginseng Research
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    • v.45 no.2
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    • pp.218-227
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    • 2021
  • Background: Panax ginseng is one of the most important medicinal plants and is usually harvested after 5 to 6 years of cultivation in Korea. Heavy metal (HM) exposure is a type of abiotic stress that can induce oxidative stress and decrease the quality of the ginseng crop. Siderophore-producing rhizobacteria (SPR) may be capable of bioremediating HM contamination. Methods: Several isolates from ginseng rhizosphere were evaluated by in vitro screening of their plant growth-promoting traits and HM resistance. Subsequently, in planta (pot tests) and in vitro (medium tests) were designed to investigate the SPR ability to reduce oxidative stress and enhance HM resistance in P. ginseng inoculated with the SPR candidate. Results: In vitro tests revealed that the siderophore-producing Mesorhizobium panacihumi DCY119T had higher HM resistance than the other tested isolates and was selected as the SPR candidate. In the planta experiments, 2-year-old ginseng seedlings exposed to 25 mL (500 mM) Fe solution had lower biomass and higher reactive oxygen species level than control seedlings. In contrast, seedlings treated with 108 CFU/mL DCY119T for 10 minutes had higher biomass and higher levels of antioxidant genes and nonenzymatic antioxidant chemicals than untreated seedlings. When Fe concentration in the medium was increased, DCY119T can produce siderophores and scavenge reactive oxygen species to reduce Fe toxicity in addition to providing indole-3-acetic acid to promote seedling growth, thereby conferring inoculated ginseng with HM resistance. Conclusions: It was confirmed that SPR DCY119T can potentially be used for bioremediation of HM contamination.

Changes in Temperature and Quality during Fermentation Period of Traditional Doenjang in Gyeonggi Province (경기지역 전통 된장의 발효 기간 중 온도와 품질 변화)

  • Lee, Yong-Seon;Cho, Chang-Hui;Seo, Jae-Soon;Lee, Dae-Hyoung;Kang, Heui-Yun
    • The Korean Journal of Food And Nutrition
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    • v.33 no.6
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    • pp.631-638
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    • 2020
  • This study was conducted to investigate the temperatures inside and outside the pot as well as the quality characteristics of traditional Doenjang made by seven different farmers, in the Gyeonggi province during fermentation. The outside temperature of each region in the province was 5 to 10℃ higher in one place, in which fermentation took place inside the glass greenhouse, compared to other regions, while six other places showed similar changes in temperature. The moisture contents of the samples decreased according to the progress of the fermentation period from 53.6~62.3% to 51.0~58.5%. The salinity generally increased from 9.7~14.2% to 10.6~16.3% except for samples B and D, which declined from 17.0~17.2% to 16.6~16.9%. The amino-type nitrogen contents increased from 180.3~557.8 mg% to 437.3~840.7 mg%. The ammonia-type nitrogen contents decreased from 116.3~561.9 mg% to 70.7~149.2 mg%. Overall, the color of Doenjang was similar, but sample E had higher a and b values than the other regions, and the total bacterial count in the entire region was similar.

One-pot synthesis of silica-gel-based adsorbent with Schiff base group for the recovery of palladium ions from simulated high-level liquid waste

  • Wu, Hao;Kim, Seong-Yun;Ito, Tatsuya;Miwa, Misako;Matsuyama, Shigeo
    • Nuclear Engineering and Technology
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    • v.54 no.10
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    • pp.3641-3649
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    • 2022
  • A simple solvothermal reaction was used to prepare a 3-aminopropyl-functionalized silica-gel-based adsorbent for adsorbing Pd(II) from the nitric acid solution. Scanning electron microscopy, fourier transform infrared spectroscopy, and thermogravimetry analysis were performed on the as-synthesized adsorbent to demonstrate the successful introduction of Schiff base groups. Batch experiments were used to investigate the effects of contact time, nitric acid concentration, solution temperature, and adsorption capacity. It is worth noting that the prepared adsorbent exhibited a higher affinity toward Pd(II) with the uptake approximately 100% even in a 2 M HNO3 solution. At an equilibrium time of 5 h, the maximum adsorption capacity of Pd(II) was estimated to be 0.452 mmol/g. The adsorbed Pd(II) could be completely eluted by dissolving 0.2 M thiourea solution in 0.1 M HNO3. Using a combination of particle-induced X-ray emission analysis and an X-ray photoelectron spectrometer, the adsorbed Pd was found to be uniformly distributed on the surface of the prepared adsorbent and the existing species were Pd(II) and zero-valent Pd(0). Due to the desirable performances, facile preparation method, and abundant raw material source, the prepared adsorbent demonstrated a high application potential in the recovery of Pd(II) from simulated high-level liquid waste treatment.

Metal-organic frameworks-driven ZnO-functionalized carbon nanotube fiber for NO2 sensor

  • Woo, Sungyoon;Jo, Mingyeong;Lee, Joon-Seok;Choi, Seung-Ho;Lee, Sungju;Jeong, Hyeon Su;Choi, Seon-Jin
    • Journal of Sensor Science and Technology
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    • v.30 no.6
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    • pp.369-375
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    • 2021
  • In this study, heterogeneous ZnO/CNTF composites were developed to improve the NO2-sensing response, facilitated by the self-heating property. Highly conductive and mechanically stable CNTFs were prepared by a wet-spinning process assisted by the liquid crystal (LC) behavior of CNTs. Metal-organic frameworks (MOFs) of ZIF-8 were precipitated on the surface of the CNTF (ZIF-8/CNTF) via one-pot synthesis in solution. The subsequent calcination process resulted in the formation of the ZnO/CNTF composites. The calcination temperatures were controlled at 400, 500, and 600 ℃ in an N2 atmosphere to confirm the evolution of the microstructures and NO2-sensing properties. Gas sensor characterization was performed at 100 ℃ by applying a DC voltage to induce Joule heating through the CNTF. The results revealed that the ZnO/CNTF composite after calcination at 500 ℃ (ZnO/CNTF-500) exhibited an improved response (Rair/Rgas = 1.086) toward 20 ppm NO2 as compared to the pristine CNTF (Rair/Rgas = 1.063). Selective NO2-sensing properties were demonstrated with negligible responses toward interfering gas species such as H2S, NH3, CO, and toluene. Our approach for the synthesis of MOF-driven ZnO/CNTF composites can provide a new strategy for the fabrication of wearable gas sensors integrated with textile materials.

Compositions of fatty acids and structural identification in human breast milk (모유 중 지방산 조성 및 구조 분석)

  • Ji-Woo, Yoo;Young-Soo, Keum;Nam Mi, Kang
    • Analytical Science and Technology
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    • v.35 no.6
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    • pp.229-236
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    • 2022
  • Lipidomic analyses of transient breast milk are far more limited than those of other dairy products. As a preliminary analysis of breast milk lipidomes, analytical methods for polar and nonpolar lipids from transient breast milk were developed, and detailed fatty acid profiles were determined in this study. The newly developed methods include solvent fractionation of phospholipids and acyl glycerol, one-pot derivatization to FAMEs and pyridylcarbinol esters, and instrumental analysis, including GC-FID and GC-MS. The results indicate that breast milk contains 16 major common fatty acids with 8-22 carbons. Additionally, 29 minor fatty acids were identified, including odd-numbered fatty acids and branched analogues with 11-23 carbons. Their detailed concentrations in different fractions were measured using the internal standard method. In addition to ordinary fatty acids, breast milk contains several branched fatty acids, including iso/anteiso acids with 15-18 carbons. Structural studies have been performed on selected minor fatty acids via chemical synthesis.

Reduced use of nitrogen fertilizer through retarded hydrolysis of urea by pyroligneous acid for Chinese cabbage cultivation (배추 재배 시 목초액에 의한 요소 가수분해 지연을 통한 질소비료 절감 효과)

  • Lee, Joo-Kyung;Park, Hyun Jun;Park, Jin Hee
    • Journal of Applied Biological Chemistry
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    • v.65 no.1
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    • pp.1-6
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    • 2022
  • Urea is one of the most common nitrogen fertilizer, but nitrogen use efficiency by crop is low because of rapid hydrolysis of urea and loss of nitrogen in environments. Therefore, it is important to control the nitrogen release from nitrogen fertilizers. In this study, pyroligneous acid (PA) was used as a mean to inhibit urease in soil and prevent excessive nitrogen release from urea. Active ingredient in PA (AI) inhibited ammonification of urea in soil by reducing extracted ammonium nitrogen at 79.7% compared to the soil without PA. In order to evaluate the effect of PA on fertilization efficiency of urea, Chinese cabbage (Brassica campestris var. Pekinensis) was cultivated in soil treated with urea and PA both in pot and field. For PA treatment, half amount of urea was used compared to the amount of urea conventionally applied to Chinese cabbage. The PA treatment with half amount of urea resulted in similar Chinese cabbage biomass to the conventional urea application. Nitrogen concentration in Chinese cabbage was less in PA treatment indicating that Chinse cabbage effectively used nitrogen. Consequently, fertilization of urea with PA will reduce amount of fertilizer and frequency of application.

Synthesis of Ce-doped In2O3 nanoparticles via a microwave-assisted hydrothermal pathway and their application as an ultrafast breath acetone sensor

  • Byeong-Hun Yu;Sung Do Yun;Chan Woong Na;Ji-Wook Yoon
    • Journal of the Korean institute of surface engineering
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    • v.56 no.6
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    • pp.393-400
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    • 2023
  • Acetone, a metabolite detected from the exhaled breath of people doing a diet, can be used for non-invasive monitoring of diet efficiency. Thus, gas sensors with rapid response and recovery characteristics to acetone need to be developed. Herein, we report ultrafast acetone sensors using Ce-doped In2O3 nanoparticles prepared by the one-pot microwave-assisted hydrothermal method. The pure In2O3 sensor shows a high response and fast response time (τres = 6 s) upon exposure to 2 ppm acetone at 300 ℃, while exhibiting a relatively sluggish recovery speed (τrecov = 1129 s). When 20 wt% Ce is doped, the τrecov of the sensor significantly decreased to 45 s withholding the fast-responding characteristic (τres = 6 s). In addition, the acetone response (resistance ratio, S) of the sensor is as high as 5.8, sufficiently high to detect breath acetone. Moreover, the sensor shows similar acetone sensing characteristics even under a highly humid condition (relative humidity of 60%) in terms of τres (6 s), τrecov (47 s), and S (4.7), demonstrating its high potential in real applications. The excellent acetone sensing characteristics of Ce-doped In2O3 nanoparticles are discussed in terms of their size, composition, phase, and oxygen adsorption on the sensing surface.

Development of Light Distributed Solar Modules for Growing Sprout Ginseng and Overseas Empirical Research on Natural Lighting Cultivation System (새싹인삼 재배를 위한 투광 분산형 태양광 모듈 개발 및 자연채광 재배시스템 해외 실증연구)

  • Seong-Hwan Kang;Jae-ho Choi;In-Sung Jung;Gyeong-Yeol Lim;Jae-Woo Park;Nguyen Huu Hoang;Chong-Yeal Kim
    • Current Photovoltaic Research
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    • v.12 no.1
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    • pp.24-30
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    • 2024
  • To create an environment for growing ginseng sprouts, we developed a solar module that partially transmits and disperses light. A G to G type light transmission and dispersion solar module was developed using glass with a mist pattern applied, and the light dispersion effect of the developed module was confirmed through illuminance measurement. The output of one module is approximately 260 W, and the configuration consists of 48 cells in series in 4 strings. The cultivation system where the developed module will be installed was developed in the form of a container, and three units of 2.6 kW (260 W x 10 EA). The inside of the cultivation system consists of a shading screen, air conditioner, ventilator, plastic pot, etc. to create an environment for cultivating sprout ginseng. As a result of actually planting sprout ginseng, it was confirmed through verification that the plants were grown without any problems.

Study on the yield and delayed stem senescence of soybean varieties in late sowing cultivation

  • Suzuki, Daisuke;Gunji, Kento;Higo, Masao;Isobe, Katsunori
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.201-201
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    • 2017
  • Delayed stem senescence of soybean is a phenomenon of retarded leaf and stem yellowing, where plants maintain a high stem water content and remain chlorophyll in leaf and stem at maturity stage. This phenomenon was one of the most important physiological disease in Japanese soybean cultivation. The occurrence of delayed stem senescence was affected by sowing time. And the most of Japanese field, soybean seeds were sowed in June. June is the rainy season in Japan, and the soil water content of field become higher in this season. In this study, the effects of late sowing (July sowing) on the yield and the occurrence of delayed stem senescence in soybean cultivars Enrei, Tachinagaha and Ayakogane were examined from 2013 to 2015, in the experimental farm at Nihon University (Fujisawa-city, Kanagawa, Japan). The seeds of all cultivars were sowed in June (June-normal density plot) or July (July-normal density plot, July-high density plot and July-super high density plot) in field experiment. The pot experiments were carried out in 2014. In all cultivars, the yield of July-high density plot and July-super high density were higher than that of June normal density plot. And the yield of June-normal density plot was the same as that of July-normal density plot. In all cultivars, the occurrence of delayed stem senescence was increased by seeding in June sowing. And in July sowing plots, no significance difference in the occurrence of delayed stem senescence was observed among density plots. One of reason about the increasing the occurrence of delayed stem senescence in June-normal plot was the increasing of the damaged seeds by bean bugs. Add one of reason about the decreasing of the occurrence of delayed stem senescence of July plots was the decreasing of the amount of cytokinin supplied from root to top and water stress after the flowering time was improved compared with the June plot. In conclusion, the yield of Enrei, Tachinagaha and Ayakogane were not changed by changing the sowing time from June to July. In all cultivars, the occurrence of delayed stem senescence were decreasing by seeding in July.

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Characteristics of Storm Waves at Gangneung port Based on the Wave Hindcasting (파랑 후측 모의 실험 기반 강릉항 폭풍파랑 분석)

  • Ahn, Kyungmo;Hwang, Soon-mi;Chun, Hwusub
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.28 no.6
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    • pp.375-382
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    • 2016
  • In the present study, the wave hindcasting has been performed, and then the characteristics of storm waves at Gangnueng port was investigated, in which the high waves are observed. Comparing the numerical results with the wave measurements at Gangneung port, Niigata, and Hamada, there were good agreements between them. In particular, the Pearson correlation coefficients of significant wave heights and peak periods at Gangneung port were 0.92 and 0.72, respectively. Then the extreme wave analysis on the significant wave heights was carried out for the estimation of the frequency of storm waves. In this analysis, the storm waves over the threshold were fitted to GPD(Generalized Pareto Distribution). According to this analysis, the return period of the storm wave on February, 24, 2008, one of the large storm waves at Gangneung port, was 8.2 months. Among the computed significant wave heights larger than one-year wave, 58.3% of them were resulted from the storm, while the others were from the typhoon. Additionally, the regression analysis on the waves larger than one-month wave has been conducted, and then the relationship between the computed significant wave heights and the significant wave period, $T_{1/3}=7H_s^{0.25}$ was obtained.