• Journal of Sensor Science and Technology
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• v.32 no.4
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• pp.252-256
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• 2023

A one-step method for nanoscale patterning of silver ionic ink on a substrate is developed using a microscale, elastic mold deformation. This method yields unique micro/nanoscale metallic structures that differ from those produced using the original molds. The linewidth of these metallic structures is significantly reduced (approximately 10 times) through the sidewall deformation of the original mold cavity on a thin liquid film, as verified by finite element analysis. The process facilitates the fabrication of various, isolated and complex micro/nanoscale metallic structures with negligible residual layers at low cost and high throughput. These structures can be utilized for various applications, including optoelectronics, wearable sensors, and metaverse-related devices. Our approach offers a promising tool for manipulation and fabrication of micro/nanoscale structures of various functional materials.

• Coupled systems mechanics
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• v.3 no.1
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• pp.1-25
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• 2014

In this work, we present the theoretical formulation, operator split solution procedure and partitioned software development for the coupled thermomechanical systems. We consider the general case with nonlinear evolution for each sub-system (either mechanical or thermal) with dedicated time integration scheme for each sub-system. We provide the condition that guarantees the stability of such an operator split solution procedure for fully nonlinear evolution of coupled thermomechanical system. We show that the proposed solution procedure can accommodate different evolution time-scale for different sub-systems, and allow for different time steps for the corresponding integration scheme. We also show that such an approach is perfectly suitable for parallel computations. Several numerical simulations are presented in order to illustrate very satisfying performance of the proposed solution procedure and confirm the theoretical speed-up of parallel computations, which follow from the adequate choice of the time step for each sub-problem. This work confirms that one can make the most appropriate selection of the time step with respect to the characteristic time-scale, carry out the separate computations for each sub-system, and then enforce the coupling to preserve the stability of the operator split computations. The software development strategy of direct linking the (existing) codes for each sub-system via Component Template Library (CTL) is shown to be perfectly suitable for the proposed approach.

• Transactions of the Korean Society of Mechanical Engineers
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• v.19 no.5
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• pp.1176-1181
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• 1995

Microdrilling is one of the most difficult operations because of the poor chip discharge and the weakness of tool. This study is concerned about the development of a microdrilling monitoring system that is useful for minimizing the tool breakage and enhancing the machinability in the air spindle based microdrilling. The system is composed of a drilling state detection unit and an adaptive step-feed control unit that controls the micro-stepping motor driven spindle axis. Drilling states such as overload, tood breakage are recognized by the change of the air spindle speed which is measured via the reflective photo sensor. Based on the monitoring results, the adaptive step-feed control algorithm adjusts the step increment to keep the decrease of spindle speed within a specified range. The results of evaluation tests have shown that the developed system is very effective to prevent the breakage of microdrill and improves the productivity in comparison with the conventional microdrilling.

• Elastomers and Composites
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• v.33 no.4
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• pp.255-266
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• 1998

One-step continuous reactive blend with EPDM is investigated using a modular intermeshing corotating twin screw extruder This was performed via anionic polymerization mechanism. Mechanical properties were characterized and compared with mechanical blends.

• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1751-1757
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• 2007

Elastin-like polypeptides (ELPs) undergo a reversible inverse phase transition upon a change in temperature. This thermally triggered phase transition allows for a simple and rapid means of purifying a fusion protein. Recovery of ELPs-tagged fusion protein was easily achieved by aggregation, triggered either by raising temperature or by adding salt. In this study, levansucrase has been used as a model enzyme in the development of a simple one-step purification method using ELPs. The levansucrase gene cloned from Pseudomonas aurantiaca S-4380 was tagged with various sizes of ELPs to functionally express and optimize the purification of levansucrase. One of two ELPs, ELP[V-20] or ELP[V-40], was fused at the C-terminus of the levansucrase gene. A levansucrase-ELP fusion protein was expressed in Escherichia coli $DH5{\alpha}$ at $37^{\circ}C$ for 18 h. The molecular masses of levansucrase-ELP[V-20] and levansucrase-ELP[V-40] were determined as 56 kDa and 65 kDa, respectively. The phase transition of levansucrase-ELP[V-20] occurred at $20^{\circ}C$ in 50 mM Tris-Cl (pH 8) buffer with 3 M NaCl added, whereas the phase transition temperature ($T_t$) of levansucrase-ELP[V-40] was $17^{\circ}C$ with 2 M NaCl. Levansucrase was successfully purified using the phase transition characteristics of ELPs, with a recovery yield of higher than 80%, as verified by SDS-PAGE. The specific activity was measured spectrophotometrically to be 173 U/mg and 171 U/mg for levansucrase-ELP[V-20] and levansucrase-ELP[V-40], respectively, implying that the ELP-tagging system provides an efficient one-step separation method for protein purification.

• Bulletin of the Korean Chemical Society
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• v.35 no.9
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• pp.2665-2668
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• 2014

Aptamers are oligonucleotides or peptide molecules that are able to bind to their specific target molecules with high affinity via molecular recognition. In this study, we present development of aptamer-based precipitation assays (or simply aptamoprecipitation) for His-tagged proteins and thrombin to compare their purification efficiency with other conventional affinity precipitation methods. A crosslinking method was employed to immobilize thiol-functionalized aptamers onto the surface of polystyrene resins, enabling them to specifically bind to His-tag and to thrombin, respectively. The resulting aptamer-functionalized resins were successfully applied via a one-step experiment to purification of His-tagged proteins from complex E. coli and to thrombin extraction, exhibiting superior or at least comparable purification results to the conventional immobilized metal affinity precipitation or immunoprecipitation.

• Proceedings of the Korean Statistical Society Conference
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• 2005.05a
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• pp.161-166
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• 2005

Locus ordering is the necessary step in constructing genetic map, and the construction of reliable and fine genetic map is one of the most important issue in genetic research area. Locus ordering searches for the best locus order among the possible orders and it amounts to evaluating the maximum likelihood for each order. With only 10 loci, for example, there are 1,814,000 possible orders, and therefore, locus ordering entails a big computational problem. In this paper we suggest a useful algorithm for loci ordering via the Fiedler vector. The suggested algorithm is easy to compute and can handle many loci simultaneously. Furthermore, the required computation time is very short compared to others and the result of locus ordering is very accurate.

• 한국정보디스플레이학회:학술대회논문집
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• 2005.07a
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• pp.710-713
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• 2005

In this study, waffle type barrier ribs for counter electrode discharge cells were prepared via micro-molding process. The master mold was prepared by UV lithography and working mold was manufactured by replicating the master mold. The UV paste used in this study consisted of ceramic powders for the barrier ribs, binder, hardener, and dispersant was filled into cavities of a polymeric mold by action of capillary pressure developed between mold and paste. The results demonstrated a possibility of one-step process for the manufacturing of waffle type barrier ribs embedded with sustaining electrodes.

• Macromolecular Research
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• v.17 no.9
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• pp.697-702
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• 2009

Mesoporous ethanesilica thin film was prepared using PEO-PLGA-PEO triblock copolymers as structure-directing agents and (1,2-bis(triethoxysilyl) ethane BTESE; bridged organosilicates) as inorganic precursors via one-step sol-gel condensation of ethanesilica precursors. The mesostructure of ethanesilica films is critically dependent on the processing experimental parameters after the hydrolyzed silica sol mixture was spin-cast. This study examined the effects of the block copolymer template/organosilica precursor ratio in the casting solution and aging period before calcination of the mesostructure. It was further demonstrated that mesoscopic ordering of organosilicate thin films is induced by the rearrangement of block copolymer template/organosilica hybrid during thermal decomposition of the PEO-PLGA-PEO triblock copolymer. The mesoporous structure and morphology were characterized by SAXS, TEM and solid-state NMR measurement.

• International Journal of Automotive Technology
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• v.8 no.3
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• pp.259-268
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• 2007

A knock detection circuit that is based on the signal of an accelerometer installed on the engine block of a spark ignition automotive engine has a band-pass filter with a certain frequency as a parameter to be calibrated. A new statistical method for the determination of the frequency which is the most suitable for the knock detection in real-time applications is proposed. The method uses both the cylinder pressure and block vibration signals and is divided into two steps. In both steps, a new recursive trigonometric interpolation method that calculates the frequency contents of the signals is applied. The new trigonometric interpolation method developed in this paper improves the performance of the Discrete Fourier Transformation, allowing a flexible choice of the size of the moving window. In the first step, the frequency contents of the cylinder pressure signal are calculated. The knock is detected in the cylinder of the engine cycle for which at least one value of the maximal amplitudes calculated via the trigonometric interpolation method exceeds a threshold value indicating a considerable amount of oscillations in the pressure signal; this cycle is selected as a knocking cycle. In the second step, the frequency analysis is performed on the block vibration signal for the cycles selected in the previous step. The knock detectability, which is an individual cylinder attribute at a certain frequency, is verified via a statistical hypothesis test for testing the equality of two mean values, i.e. mean values of the amplitudes for knocking and non-knocking cycles. Signal-to-noise ratio is associated in this paper with the value of t-statistic. The frequency with the largest signal-to-noise ratio (the value of t-statistic) is chosen for implementation in the engine knock detection circuit.