• KIPS Transactions on Computer and Communication Systems
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• v.7 no.12
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• pp.301-312
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• 2018

In industrial wireless sensor networks, the continuous object detection such as fire or toxic gas detection is one of major applications. A continuous object occurs at a specific point and then diffuses over a wide area. Therefore, many studies have focused on accurately detecting a continuous object and delivering data to a static sink with an energy-efficient way. Recently, some applications such as fire suppression require mobile sinks to provide real-time response. However, the sink mobility support in continuous object detection brings challenging issues. The existing approaches supporting sink mobility are designed for individual object detection, so they establish one-to-one communication between a source and a mobile sink for location update. But these approaches are not appropriate for a continuous object detection since a mobile sink should establish one-to-many communication with all sources. The one-to-many communication increases energy consumption and thus shortens the network lifetime. In this paper, we propose the origin-centric communication scheme to support sink mobility in a continuous object detection. Simulation results verify that the proposed scheme surpasses all the other work in terms of energy consumption.

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.54 no.8
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• pp.378-383
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• 2005

We report the development of miniature fluorescence detection systems that employ miniature prism, mirrors and low coat CCD camera to detect the fluorescence emitted from 40 fluorescently-labeled protein patterns without scanner. This kind of miniature fluorescence detection system can be used in point of care. We introduce two systems, one uses prism+mirror block and the other uses prism and two mirrors. A large NA microscope eyepiece and low cost CCD camera are used. We fabricated protein chip containing multi-pattern BSA labeled with Cy5, using MEMS technology and modified the surface chemically to clean and to immobilize proteins. The measurements show that the combination of prism and mirrors can homogenize elliptical excitation light over the sample with higher optical efficiency, and increase the separation between excitation and fluorescence light at the CCD to give higher signal intensity and higher signal to noise ratio. The measurements also show that protein concentrations ranging from 10 ng/ml to 1000 ng/ml can be assayed with very small error. We believe that the proposed fluorescence detection system can be refined to build a commercially valuable hand-held or miniature detection device.

• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1323-1329
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• 2021

Micro-scale magnetic beads are widely used for isolation of proteins, DNA, and cells, leading to the development of in vitro diagnostics. Efficient isolation of target biomolecules is one of the keys to developing a simple and rapid point-of-care diagnostic. A zinc finger protein (ZFP) is a double-stranded (ds) DNA-binding domain, providing a useful scaffold for direct reading of the sequence information. Here, we utilized two engineered ZFPs (Stx2-268 and SEB-435) to detect the Shiga toxin (stx2) gene and the staphylococcal enterotoxin B (seb) gene present in foodborne pathogens, Escherichia coli O157 and Staphylococcus aureus, respectively. Engineered ZFPs are immobilized on a paramagnetic bead as a detection platform to efficiently isolate the target dsDNA-ZFP bound complex. The small paramagnetic beads provide a high surface area to volume ratio, allowing more ZFPs to be immobilized on the beads, which leads to increased target DNA detection. The fluorescence signal was measured upon ZFP binding to fluorophore-labeled target dsDNA. In this study, our system provided a detection limit of ≤ 60 fmol and demonstrated high specificity with multiplexing capability, suggesting a potential for development into a simple and reliable diagnostic for detecting multiple pathogens without target amplification.

• Journal of the Korea Society for Simulation
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• v.7 no.1
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• pp.27-37
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• 1998

Simulation output is generally stochastic and autocorrelated, and includes the initial condition bias. To exclude the bias, the determination of truncation point has been one of important issues for the steady-state simulation output analysis. In this paper, two methods are presented for detection of truncation point in order to estimate efficiently the steady-state measure of simulation output. They are based on the Euclidean distance equation, and the backpropagation algorithm in Neural Networks. The experimental results obtained by M/M/1 and M/M/2 show that the proposed methods are very promising with respect to coverage and relative bias. The methods could be used for the on-line analysis of simulation outputs.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.8
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• pp.1424-1429
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• 2007

This paper presents a simple and reliable one-touch type multi-immunosensing lab-on-a-chip (LOC) detecting antibodies as multi-disease markers using electrochemical method suitable for a portable point-of-care system (POCS). The multi-stacked LOC consists of a PDMS space layer for liquids loading, a PDMS valve layer with 50 im in height for the membrane, a PDMS channel layer for the fluid paths, and a glass layer for multi electrodes. For the disposable immunoassay which needs sequential flow control of sample and buffer liquids according to the designed strategies, reliable and easy-controlled on-chip operation mechanisms without any electric power are necessary. The driving forces of sequential liquids transfer are the capillary attraction force and the pneumatic pressure generated by air bladder push. These passive fluid transport mechanisms are suitable for single-use LOC module. Prior to the application of detection of the antibody as a disease marker, the model experiments were performed with anti-DNP antibody and anti-biotin antibody as target analytes. The flow test results demonstrate that we can control the fluid flow easily by using the capillary stop valve and the PDMS check valves. By the model tests, we confirmed that the proposed LOC is easily applicable to the bioanalytic immunosensors using bioelectrocatalysis.

• Journal of the Korean Institute of Intelligent Systems
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• v.24 no.1
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• pp.28-32
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• 2014

For a signal such as speech showing piece-wise linear shape in a very short time period, a nonuniform sampling method based on the inflection point detection (IPD) is proposed to reduce data rate. The method exploits the geometrical characteristics of signal further than the existing local maxima/minima detection (MMD) based sampling method. As results, the reconstructed signal by the interpolation of the IPD based sampled data resembles the original speech more. Computer simulation shows that the proposed IPD based method produces about 9~23 dB improvement over the existing MMD method. To show the usefulness of the IPD technique, it is applied to speech coding, and compared to the continuously variable slope delta modulation (CVSD). The nonuniformly sampled data is binary coded with one bit flag set "1". Noninflection samples are not sent, but only flag bits set 0 are sent. The method shows 0.3 ~ 9 dB SNR and 0.5 ~ 1.3 mean opinion score (MOS) improvements over the CVSD.

• BMB Reports
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• v.29 no.5
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• pp.442-447
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• 1996

Mutations in codon 12, 13 and 61 of one of the three ras genes, H-ras, K-ras and N-ras, convert these genes into active oncogenes. The presence of H-ras gene mutations have important prognostic implications in various cancers. In this study, the H-ras gene mutations were investigated by two-step PCRRFLP in patients with bladder and stomach cancer. For the control experiments, T24 and SK2 cell lines were used. In a total of 36 bladder cancer patient cases, five (13.9%) mutations were found by this method. Of these, point 12 mutations were two (5.6%) cases and point 61 mutations were three (8.3%) cases. On the other hand, H-ras mutation was not found in 29 cases of stomach cancer. The results of the mutated H-ras gene confirmed by direct sequencing analysis were correlated well with PCR analysis. From the sensitivity test, the H-ras mutation was found to have about 0.2% of mutated DNA mingled in normal DNA. In conclusion, the H-ras mutation has a higher clinical Significance in bladder cancer than stomach cancer. Moreover the two-step PCR-RFLP method is sensitive, rapid and relatively simple for clinical work in detecting H-ras point mutations.

• Journal of Navigation and Port Research
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• v.44 no.2
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• pp.110-118
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• 2020

With the expansion of the shipping and port logistics industry in the 21st century, the traffic density is continuously increased because of the increase in volumes of world sea freight and fleets, as well as the increase in the causes of potential marine accidents, such as ship collisions and stranding. Accordingly, the International Maritime Organization (IMO) has requested that the installation and operation of VTS should be applied in areas with high risk of marine traffic, and the request should be included as one of the Safety of Life at Sea (SOLAS) regulations. In this paper, the fundamental requirements of the radar system for vessel traffic services were analyzed and the analyzing factors were based on the IALA guideline.s This paper also includes results for the requirement and recommendation analysis on detection distance, target separation, and the target position accuracy of X-band radar. Also, to check if it satisfies the requirement of detection distance, range and azimuth separation of small point targets, and target position accuracy from the IALA guidelines, the test was conducted through the radar image acquired at the VTS center, and hence, the validity of the technical performance requirements was confirmed.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.21 no.10
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• pp.1551-1560
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• 1997

This study is devoted to the detection of the 3-dimensional point obstacles on the plane by using accumulated scan line images. The proposed accumulating only one scan line allow to process image at real time. And the change of motion of the feature in image is small because of the short time between image frames, so it does not take much time to track features. To obtain recursive optimal obstacles position and robot motion along to the motion of camera, Kalman filter algorithm is used. After using Kalman filter in case of the fixed environment, 3-dimensional obstacles point map is obtained. The position and motion of moving obstacles can also be obtained by pre-segmentation. Finally, to solve the stereo ambiguity problem from multiple matches, the camera motion is actively used to discard mis-matched features. To get relative distance of obstacles from camera, parallel stereo camera setup is used. In order to evaluate the proposed algorithm, experiments are carried out by a small test vehicle.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.10a
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• pp.538-539
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• 2018

This paper summarizes the implementation of lane recognition using OpenCV, one of the open source computer vision libraries. The Linux operating system Rasbian(r18.03.13) was installed on the ARM processor-based Raspberry Pi 3 board, and Raspberry Pi Camera was used for image processing. In order to realize the lane recognition, Canny Edge Detection and Hough Transform algorithm implemented in OpenCV library was used and RANSAC algorithm was used to prevent shaking of vanishing point and to detect only the desired straight line. In addtion, the DC motor and the Servo motor were controlled so that the vehicle would run according to the detected lane.