• Journal of Life Science
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• v.21 no.1
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• pp.134-140
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• 2011

One hundred forty five bacterial colonies which were able to degrade crude oil were isolated from soil samples that were contaminated with oil in the Daejon area. Among these colonies, one bacterial strain was selected for this study based on its low surface tension ability, and this selected bacterial strain was identified as Pseudomonas sp. Z1 through physiological-biochemical tests and analysis of its 16S rRNA sequence. Pseudomonas sp. Z1 showed a high resistance to antibiotics such as chloramphenicol and ampicillin, as well as heavy metals such as lithium, manganese, and barium. It was found that the optimal pH and temperature for biosurfactant production of Pseudomonas sp Z1 were pH 6.0-7.0 and $30^{\circ}C$, respectively. After ten hours of inoculation, the biosurfactant activity of the culture broth decreased rapidly, and had maximum surface tension (28 dyne/cm) after twenty-one hours incubation. The biosurfactant activity of the culture broth was also decreased up to 2% NaCl concentration.

• Molecular & Cellular Toxicology
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• v.1 no.3
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• pp.189-195
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• 2005

Industrial development has increase consumption of crude oil and environmental pollution. A large number of microbial lipolytic enzymes have been identified and characterized to date. To development for a new lipase with catalytic activity in degradation of crude oil as a microbial enzyme, Acinetobactor sp. B2 was isolated from soil samples that were contaminated with oil in Daejon area. Acinetobactor sp. B2 showed high resistance up to 10 mg/mL unit to heavy metals such as Ba, Li, Al, Cr, Pb and Mn. Optimal growth condition of Acinetobactor sp. B2 was confirmed $30^{\circ}C$. Lipase was purified from the supernatant by Acinetobactor sp. B2. Its molecular mass was determined to the 60 kDa and the optimal activity was shown at $40^{\circ}C$ and pH 10. The activation energies for the hydrolysis of p-nitrophenyl palmitate were determined to be 2.7 kcal/mol in the temperature range 4 to $37^{\circ}C$. The enzyme was unstable at temperatures higher than $60^{\circ}C$. The Michaelis constant $(K_{m})\;and\;V_{max}$ for p-nitrophenyl palmitate were $21.8{\mu}M\;and\;270.3{\mu}M\;min^{-1}mg\;of\;protein^{-1}$, respectively. The enzyme was strongly inhibited by $Cd{2+},\;Co^{2+},\;Fe^{2+},\;Hg^{2+},\;EDTA$, 2-Mercaptoethalol. From these results, we suggested that lipase purified from Acinetobactor sp. B2 should be able to be used as a new enzyme for degradation of crude oil, one of the environmental contaminants.

• The Research Journal of the Costume Culture
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• v.14 no.5
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• pp.828-839
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• 2006

These days, by the accelerated developments of science and industries and the menaces of war and terror, humans have come to have instinct to recur and nostalgia to nature and thereby many art works and designs with natural objectives have begun to be produced. Also the design in 21st century requires creative products based on cultural speciality of the countries. As the examples, we may easily find the images of the countries from visual expressions or products when we get in touch with the products of so called advanced countries in design i. e. U.S.A. Japan, Germany, France, Italy, countries of Scandinavian peninsular. This study, in such stream of the times, was to express our culture's own originality on clothing design with butterfly that is one of natural thing as the material but limited the boundary to realistic butterflies in folk drawings of the times of Chosun. As for the technique of expression, used digital textile printing for best expression of the fine lines, realistic shapes and peculiar colors of the butterflies in Chosun folk drawings and used diverse materials as materials for printing for diversity of the designs. Thereby this thesis is purposed to grope the products with superior competitiveness in the world market by presenting the realistic butterflies in Chosun folk drawings as motives with cultural value native to Korea and applying them to clothing designs.

• Korean Journal of Food Science and Technology
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• v.9 no.4
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• pp.313-316
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• 1977

The patterns of saponins of lateral gingengs cultivated different areas and various ginseng products were investigated by quantitative thin-layer chromatography. In the case of ginseng cultivated in the Kum San and Gang Hwa area, some parts of the panaxatriol series of the saponins (peak 6 and 7.8.9) were higher in concentration than in ginseng grown in other areas while the other ingredients were almost the same. In the process of heat treatment the quantity of peak 2 was generally decreased. However, in the case of red and white ginseng, one part of the panaxatriol saponins, peak 6 was increased. This tendency was also found in honeyed ginseng and ginseng tea which were not exposed to sunlight, but the increase was much less. The change in the red and white ginseng which were exposed to sunlight was very substantial. Therefore we can assume that the increase of peak 6 comes about due to the combination of heat treatment and exposure to sunlight, especially due to exposure to sunlight.

• Journal of the Korean earth science society
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• v.21 no.3
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• pp.219-229
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• 2000

Constructive learning is an active process of meaning construction and students decide their individual learning objectives according to their own interest concern and ability. The purpose of this study is to develop a teaching-learning model and classroom materials from constructivist perspectives and to apply them to an elementary school classroom in Seoul for one month. In this study, it was reorganized the contents of unit of 'beds and fossils' based on the discussion between students and teacher during the second semester of 4th grade class. The teaching-learning model consists of five steps: 1) introduction; 2) exercise; 3) presentation; 4) consensus; and 5) development. The implementation results were summarized.

• Journal of Korean Institute of Industrial Engineers
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• v.2 no.1
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• pp.37-44
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• 1976

It is necessary for developing countries to increase their consolidation of domestic technology in order to improve their economy. In order to raise their techniques, they have to try to induce the advanced know-how from other countries in spite of heavy cost. The object of this study is to establish the model on which we base our choice of the proper techniques or plants and give priority to them by using quantified selection criteria. The method in this study has two stages, and the writer has selected 12 factors affecting the decision making for the importation of technology from the industrially advanced countries. First, the lists of valuable know-how for the better development of national industry should be determined, and for the formulation and arrangement of the lists, a council of specialists which uses questionnaires in terms of the Semantic Differential Method, should be organized. Second, for the assignments of priority to the prospective items for importation, the writer has imployed both the Leontief Model and the Disman Model as objective methods and Mottley-Newton method, one of the R&D Project Selection Methods, as a general model. The writer has applied the methods described above to the fields of petrochemical industry in Korea.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.8
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• pp.1818-1827
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• 2004

This paper carried out numerical calculation about the directivity synthesis problem in two dimension of adaptive ultrasonic transducers. Adaptive system for directivity synthesis is constructed by multi-electrode array on the surface of only one piezoelectric ceramic plate combined with optimal algorithm. In order to realize the desired directivity that is established arbitrarily, the optimal vibrational displacement is calculated by optimal algorithm (DFP method). Secondly, the optimal voltage of electrodes which is correspond to the calculated vibrational displacement is calculated used by finite element method and DFP method. And, the vibration analysis in two dimension of piezoelectric transducer carried out by means of finite element method.

• Journal of Korean Society of Rural Planning
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• v.12 no.2 s.31
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• pp.65-73
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• 2006

This study attempts to develop an interim evaluation system for the Integrated Rural Village Cluster Development Project(IRVCDP) which can secure its identity and sustainability, and induce its early settlement, and to derive plans for its steady progress. The evaluation system consists of three levels. The highest-order level is evaluation purposes, which describe IRVCDP's goals. They are expansion of income bases(EIB), improvement of living environments(ILE), strengthening of regional capabilities(SRC) and constructing effective project operation system(CEPOS). The second level is evaluation items, which each characterize the relevant evaluation purposes. The last level is evaluation indices, which observe achievement f3r evaluation purposes. 22 evaluation indices are selected to satisfy the purposes of this study through a brainstorming process and a survey on opinions of experts for those evaluation purposes and evaluation items which are derived from analyzing the IRVCDP's properties. The relative importance of the evaluation indices selected are determined through the AHP analysis. The most intriguing result from the AHP analysis is that strengthening of regional capabilities is found to be the most important one among evaluation purposes. It can be said that this result is parallel to a ultimate goal of the IRVCDP.

• Journal of the Korean Institute of Rural Architecture
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• v.12 no.3
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• pp.19-26
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• 2010

This study is aimed to look into the craftsmanship non-educated constructors in Korean-style secondary stations(Gong-So) of Naepo region which is located in the northwestern Chungcheongnam-do. Although the area was persecuted as one of three cradles of Korean Catholicism, Catholic activities flourished there. The 13 cases of Korean-style secondary stations were selected through a survey report list about modern cultural assets of Chungcheongnam-do published in december 2004. This study examined the methods of plan layout and framework of Gong-So to look into craftsmanship of non-educated constructors, the results are as follows; The methods of plan layout are divided into three types according to existence and arrangement of inner column(Nae-Ju); 'Layout with Nae-Ju' is considered as the primary form of Korean-style Gong-So with using regular Nae-Ju. 'Layout with the different position of the center between Nae-Ju and exterior column(Oe-Ju)' and 'layout without Nae-Ju' is appeared in Korean-style Gong-So which is important to assembly and alter function. The methods of framework are appeared variously according to different plan layout for securing worship space. The unusal beam(Chung-Ryang) is used to solve different spans of altar and narthex. When space was formed widely without Nae-Ju, diagonal members and M letter type rafters are used, they are influenced by the technique of western wooden roof truss.

• The Journal of Korean Society of Virology
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• v.28 no.1
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• pp.11-20
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• 1998

Twenty isolates of Hantavirus were isolated from patients and reserovirs from 1988 to 1994 in Korea. Isolation rate was 1.9% (10/538) in patients, 6.2% (5/81) in Apodemus sp., 2.6% (1/38) in Rattus sp. and 0.6% (4/677) in bats. Reciprocal mean IFA titers ranged from 27.5 to 1,024 at the specimen collection. According to the growth rate and reaching peak titier of infectivity, the isolates were grouped as rapid, intermediate, and slow growing groups. All isolates were confirmed as Hantaan type by the nested RT-PCR on the G1 region of the M segment. Comparison of nucleotide sequence (Nt: 2101 - Nt: 2280) of the G2 region revealed that the sequence homology bewteen Hantaan 76/118 virus and the isolates was more than 90%. Several nucleotide positions of the isolates showed high variation. The variation rate of patientisolates was about one-half when compared with that of rodentisolates. On the basis of phylogenetic analysis Hantaan viruses isolated were divided into two genogroups. These results indicate that Hantaan virus is highly dominant serotype in Korea and the virologic property and genogroup are not correlated.