• Journal of Life Science
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• v.21 no.8
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• pp.1149-1155
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• 2011

Myostatin (MSTN) belongs to the transforming growth factor-${\beta}$ superfamily or growth and differentiation factor 8 (GDF-8), and functions as a negative regulator of skeletal muscle development and growth. Previous studies in mammals have suggested that myostatin knock-out increased muscle mass and decreased fat content compared to those of the wide type. Recently, several studies on myostatin have beenconducted on the block myostatin signal pathway with myostatin antagonists and the MSTN regulation with RNAi to control myostatin function. This study was performed to analyze growth and muscle alteration of Oncorhychus masou by treatment with recombinant myostatin prodomains derived from fish. We designed myostatin prodomains derived from P. olivaceus (pMALc2x-poMSTNpro) and S. schlegeli (pMALc2x-sMSTNpro) in a pMALc2x expression vector, and then purified the recombinant proteins using affinity chromatography. The purified recombinant proteins were treated in O. masou through an immersion method. Recombinant protein treated groups did not show a significant difference in weight, protein, or lipid composition compared to the control. However, there was a difference in the average number and area for histological analyses in the muscle fiber. At twelve and twenty-two weeks from the initial treatment, there were differences in averagefiber number and area between the 0.05 mg/l treated-group and the control, but the numbers were similar to those of the control during the same time period. At twelve weeks, however, 0.2 mg/l treated-group had an increase in average fiber number and decrease in average fiber area compared to the control. At twenty-two weeks, the pMALc2x-sMSTNpro 0.2 mg/l treated-group was induced and showed a decrease in average fiber number and increase in average fiber area. The results between twelve and twenty-two weeks showed that the fiber numbers had decreased, whereas average fiberarea had increased due to sMSTNpro. It is understood that the sMSTNpro induced only hyperplasia at twelve weeks, after which it induced hypertrophy. Recombinant myostatin prodomains derived from fish may induce hyperplasia and hypertrophy in O. masou depending upon the time that has elapsed.

• Journal of Aquaculture
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• v.10 no.1
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• pp.49-54
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• 1997

Characteristics of sex ratio and morphometric traits of induced allotriploid between female rainbow trout, Oncorhynchus mykiss and male cherry salmon, O. masou were performed. Sex ratios in both rainbow trout and cherry salmon were equally 1:1, while hybrid and allotriploid revealed higher proportion of male offspring (p<0.01). Body trait measurements of allotriploid in head hight/head length, length of dorsal fin base/body length and length of pectoral fin/body length were intermediate to their parental species, while in length of upper jaw/head length, allotriploid much more resembled that of rainbow trout. These facts proved that allotriploidization improved characters in sex ratio and morphometric traits compared to those o their parental species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.2
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• pp.208-229
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• 1996

Comprative osteology of the chum salmon Oncorhynchus keta, masu salmon O. masou and rainbow trout O. mykiss in Korea was studied. As a result, it was found that there were some differences in the structural features of the cranium and visceral skeleton among the species. Interspecific differencies in glossohyal, supraethmoid, parasphenoid and vomer were distinctive for the species. Chum salmon could be distinguished easily by some characters on form of glossohyal which is thin, long and sharp in shape. It has triangle shape supraethmoid while other two species have oval shape one. The shape of parasphenoid, preopercle, hyomandibular and the number of epurals were evaluated as new taxonomic criteria for the salmons.