• Title/Summary/Keyword: On-the-spot detection

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Probe Classification of an On-Off Type DNA Chip Using Template Matching Method (템플릿 정합법을 이용한 온-오프 형태 DNA 칩의 탐색자 구분)

  • Ryu, Mun-Ho
    • The KIPS Transactions:PartB
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    • v.13B no.6 s.109
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    • pp.579-584
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    • 2006
  • This paper proposes a nonlinear template matching measure, called counting measure, as a signal detection measure that is defined as the number of on pixels in the spot area. It is applied to classify probes for an on-off type DNA chip, where each probe spot is classified as hybridized or not. The counting measure also incorporates the maximum response search method, where the expected signal is obtained by taking the maximum among the measured responses of the various positions and sizes of the spot template. The counting measure was compared to existing signal detection measures such as the normalized correlation and the median for 2390 patient samples tested on the human papiliomavirus (HPV) DNA chip. The counting measure performed the best regardless of whether or not the maximum response search method was used. The experimental results showed that the counting measure combined with the positional search was the most preferable.

Detection of Myrothecium Leaf Spot, A New Disease of Watermelon

  • Kim, Dong-Kil;Bae, Dong-Won;Lee, Sun-Chul;Han, Ki-Soo;Kim, Hee-Kyu
    • The Plant Pathology Journal
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    • v.19 no.4
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    • pp.200-202
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    • 2003
  • Leaf spots were first observed on watermelon (Citrullus vulgaris Schrad) under polyethylene film-covered green-house in November 2002. Symptoms appeared as dark-brown circles or large irregular spots on the leaves of watermelon. Occasionally, zonal growth of the lesions was observed. Under humid conditions, small black sclerotium-like bodies (sporodochia) were produced on the surface of the lesions. The sporodochia on leaf lesions were sessile, polymorphic, variable in size, 35-850 $\mu\textrm{m}$ in diameter, and 30-470 $\mu\textrm{m}$ in depth. Conidia in sporodochium were black in mass, one-celled, rod-shaped, with rounded ends, hyaline, guttulate, and measured 6-8$\times$1.6-2.2 $\mu\textrm{m}$ in size. The pathogen was identified as Myrothecium roridum Tode ex Fr. This is the first report of Myrothecium leaf spot on watermelon naturally occurring in commercial greenhouses.

Screening method for amines by derivatization reaction on TLC (TLC 상 유도체화 반응을 이용한 아민 계 화합물의 Screening 방법)

  • Choi, Sung-Woon;Lee, Hye-In;Sung, Nack-Do
    • Analytical Science and Technology
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    • v.26 no.4
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    • pp.228-234
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    • 2013
  • Methamphetamine is an amine-containing illegal drug and is distributed unlawfully in South Korea. Finding a rapid, convenient and semi-quantitative determination method for methamphetamine is a very important issue in the area of forensic drug testing. As an effort to develop new screening method, the reactions between three organic compounds which are structurally similar to methamphetamine and N-(9-fluorenylmethoxycarbonyloxy) succinimide (FMOC-NHS) were performed on silica gel ($SiO_2$) TLC plates. Three reference compounds were synthesized and used for the identification, comparison and study of the limit of detection (LOD) of the products obtained from a direct reaction on a TLC plate. As a result, FMOC-NHS as a derivatization reagent generated compounds containing highly UV-active functional groups on the TLC plate after reacting with primary- and secondary amines. In the experiment 2D the LOD of amines was in the range of 0.045 and 0.01 mg/mL ($2{\mu}L/spot$), and in 1D the LOD was in the range of 0.002 and 0.007 mg/mL ($2{\mu}L/spot$). The LODs of the compounds tested were dependent on the concentration of the derivatizing reagent.

Focused Electrospray Deposition for Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

  • Jeong, Kyung-Hwan;Seo, Jong-Cheol;Yoon, Hye-Joo;Shin, Seung-Koo
    • Bulletin of the Korean Chemical Society
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    • v.31 no.8
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    • pp.2293-2298
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    • 2010
  • Focused electrospray (FES) deposition method is presented for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. FES ion optics consists of two cylindrical focusing electrodes capped with a truncated conical electrode through which an electrospray emitter passes along the cylindrical axis. A spray of charged droplets is focused onto a sample well on a MALDI target plate under atmospheric pressure. The shape and size distributions of matrix crystals are visualized by scanning electron microscope and the mass spectra are obtained by time-of-flight mass spectrometry. Angiotensin II, bradykinin, and substance P are used as test samples, while $\alpha$-cyano-4-hydroxycinnamic acid and dihydroxybenzoic acid are employed as matrices. FES of a sample/matrix mixture produces fine crystal grains on a 1-3 mm spot and reproducibly yields the mass spectra with little shot-to-shot and spot-to-spot variations. Although FES greatly stabilizes the signals, the space charge due to matrix ions limits the detection sensitivity of peptides. To avoid the space charge problem, we adopted a dual FES/FES mode, which separately deposits matrix and sample by FES in sequence. The dual FES/FES mode reaches the detection sensitivity of 0.88 amol, enabling ultrasensitive of peptides by homogeneously depositing matrix and sample under atmospheric pressure.

Internal Defection Evaluation of Spot Weld Part and Carbon Composite using the Non-contact Air-coupled Ultrasonic Transducer Method (비접촉 초음파 탐상기법을 이용한 스폿용접부 및 탄소복합체의 내부 결함평가)

  • Kwak, Nam-Su;Lee, Seung-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.11
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    • pp.6432-6439
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    • 2014
  • The NAUT (Non-contact Air coupled Ultrasonic Testing) technique is one of the ultrasonic testing methods that enables non-contact ultrasonic testing by compensating for the energy loss caused by the difference in acoustic impedance of air with an ultrasonic pulser receiver, PRE-AMP and high-sensitivity transducer. As the NAUT is performed in a state of steady ultrasonic transmission and reception, testing can be performed on materials of high or low temperatures or specimens with a rough surface or narrow part, which could not have been tested using the conventional contact-type testing technique. For this study, the internal defects of spot weld, which are often applied to auto parts, and CFRP parts, were tested to determine if it is practical to make the NAUT technique commercial. As the spot welded part had a high ultrasonic transmissivity, the result was shown as red. On the other hand, the part with an internal defect had a layer of air and low transmissivity, which was shown as blue. In addition, depending on the PRF (Pulse Repetition Frequency), an important factor that determines the measurement speed, the color sharpness showed differences. With the images obtained from CFRP specimens or an imaging device, it was possible to identify the shape, size and position of the internal defect within a short period of time. In this paper, it was confirmed in the above-described experiment that both internal defect detection and image processing of the defect could be possible using the NAUT technique. Moreover, it was possible to apply NAUT to the detection of internal defects in the spot welded parts or in CFRP parts, and commercialize its practical application to various fields.

The $10-{\mu}m$ Noth-Polar Bightening of Juptier: A Dynamical Phenomenon?

  • Kim, Sang Joon;Seo, Haingja
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.1
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    • pp.60.2-60.2
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    • 2015
  • Since its detection in 1980, the $8-{\mu}m$ north-polar brightening of $CH_4$ on Juptier has not moved from $180^{\circ}$ (SysIII) longitude. The $8-{\mu}m$ $CH_4$ brightening is mostly thermal and very similar to that of $13-{\mu}m$ $C_2H_2$ emissions, but the morphology of these hydrocarbon north-polar brightenings are very different from that of the $3-{\mu}m$ $H_3{^+}$ auroral oval suggesting a significantly different excitation process yet unknown heating mechanism. Recently, Kim et al. (submitted to Icarus, 2015) found that that the center of the $3-{\mu}m$ $CH_4$ northern bright spot is located at ${\sim}200^{\circ}$ (SysIII) longitude, which is ${\sim}20^{\circ}$ west from the center of the $8-{\mu}m$ north-polar bright spot, and it does not coincide with the $3-{\mu}m$ $H_3{^+}$ bright spot. They found significantly high temperatures (500 ~ 850K) from $CH_4$ rotational lines on the $3-{\mu}m$ bright spot above the $1-{\mu}bar$ pressure level, while we find cooler temperatures (<350K) over the the $8-{\mu}m$ spot. They also found that the upper states of the $3-{\mu}m$ $CH_4$ bands are mostly populated by non-thermal excitations, such as auroral particle precipitations and/or Joule heatings in contrast to the $8-{\mu}m$ thermal emission. This finding indicates that the $10-{\mu}m$ hydrocarbon brightening is confined to low altitudes below the $1-{\mu}bar$ level eliminating the long-suggested possibility of direct auroral bombardments while opening a new possibility of dynamical origin for the $10-{\mu}m$ brightening.

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Development of Obstacle Detection System on a Railroad Crossing (철도건널목 지장물 영상검지장치 개발)

  • Cho, Bong-Kwan;Ryu, Sang-Hwan
    • Proceedings of the KIEE Conference
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    • 2009.07a
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    • pp.1197_1198
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    • 2009
  • The development of Technology study for preventing of accident and reducing the risk through the intelligence of level crossing is provide the detection of stopped car at railway crossing with the most advanced intelligence technology such as sensor, computer, communication and date processing and transmit to the operational staff on broad for reaction or make the train stopped automatically through the connection with train. Also this study include that showing the situation of crossing railway when the train is approached and prevent the accident and reduce the risk through the connection of road transit signal system. On this study is performed the test through the date from spot level crossing and the development of video detection algorism for stopped road transit vehicle at level crossing with intelligent system.

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Implementation of TFDR system with PXI type instruments for detection and estimation of the fault on the coaxial cable (동축 케이블의 결함 측정에 있어서 PXI 타입의 계측기를 이용한 개선된 TFDR 시스템의 구현)

  • Choe, Deok-Seon;Park, Jin-Bae;Yun, Tae-Seong
    • Proceedings of the KIEE Conference
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    • 2003.11b
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    • pp.91-94
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    • 2003
  • In this paper, we achieve implementation of a Time-Frequency Domain Reflectometry(TFDR) system through comparatively low performance(100MS/s) PCI extensions for Instrumentation(PXI). The TFDR is the general methodology of Time Domain Reflectometry(TDR) and Frequency Domain Reflectometry(FDR). This methodology is robust in Gaussian noises, because the fixed frequency bandwidth is used. Moreover, the methodology can get more information of the fault by using the normalized time-frequency cross correlation function. The Arbitrary Waveform Generator(AWG) module generates the input signal, and the digital oscilloscope module acquires the input and reflected signals, while PXI controller module performs the control of the total PXI modules and execution of the main algorithm. The maximum range of measurement and the blind spot are calculated according ta variations of time duration and frequency bandwidth. On the basis of above calculations, the algorithm and the design of input signals used in the TFDR system are verified by real experiments. The correlation function is added to the TDR methodology for reduction of the blind spot in the TFDR system.

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A STUDY ON THE EARLY DETECTION OF ENAMEL CARIES BY THE LUMINESCENCE EXCITED BY ARGON LASER (아르곤 레이저 광감각법의 법랑질 우식증 조기탐지 효과에 관한 연구)

  • Lee, Nan-Young;Lee, Chang-Seop;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.24 no.1
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    • pp.313-324
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    • 1997
  • The aim of the present study was to describe an safe and convenient method for the early detection of enamel caries using laser fluorescence. Fluorescence from natually carious lesion of human teeth illuminated by an argon laser(488nm) was observed and photographed using barrier filter. Intact enamel was found to fluorescence with a yellowish light. Whereas, incipient caries lesions in the enamel were dearly visible as dark areas in contrast to the fluorescence surroundings. For evaluation of accuracy of this method, lesion depth measured by the laser fluorescence in light microscope was compared with that polarizing microscope. The results from the present study can be summarized as follows : 1. Enamel caries of smooth surface was observed as pale white spot and undefined outline in ordinary light. Whereas, lesion was clearly visible as dark spot in laser fluorescence. 2. There was no difference between ordinary light view and laser fluorescence in occlusal surface and interproximal surface. 3. There was no significant difference between the lesion depth observed by laser fluorescence with light microscope and polarizing microscope. Apparent correlation exists between two groups.

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Intended for photovoltaic modules Compare modeling between SfM based RGB and TIR Images (SfM 기반 RGB 및 TIR 영상해석을 통한 태양광 모듈 이상징후 정밀위치 검출)

  • Park, Joon-Kyu;Han, Woong-ji;Kwon, Young-Hun;Kang, Joon-Oh;Lee, Yong-Chang
    • Journal of Urban Science
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    • v.8 no.1
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    • pp.7-14
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    • 2019
  • Recently, interest in solar energy, which is the center of new government energy policy, is increasing. However, the focus is on mass production of solar power plants, and policies and related technologies for maintenance and management of existing installed PV modules are insufficient. In this study, we use UAV (Unmanned Aerial Vehicle) to acquire RGB and infrared images, apply it to the structure-from-motion (SfM) based image analysis tool, model the three- And the position of the hot spot was monitored and coordinates were detected. As a result, it is possible to provide basic spatial information for maintenance of solar module by monitoring and position detection of hot-spot suspected solar cells by superimposing infrared image and RGB image based on unmanned aerial vehicle.