• The Plant Pathology Journal
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• v.34 no.3
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• pp.191-198
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• 2018

Fast and accurate diagnosis is needed to eradicate and manage economically important and invasive diseases like fire blight. Loop-mediated isothermal amplification (LAMP) is known as the best on-site diagnostic, because it is fast, highly specific to a target, and less sensitive to inhibitors in samples. In this study, LAMP assay that gives more consistent results for on-site diagnosis of fire blight than the previous developed LAMP assays was developed. Primers for new LAMP assay (named as DS-LAMP) were designed from a histidine-tRNA ligase gene (EAMY_RS32025) of E. amylovora CFBP1430 genome. The DS-LAMP amplified DNA (positive detection) only from genomic DNA of E. amylovora strains, not from either E. pyrifoliae (causing black shoot blight) or from Pseudomonas syringae pv. syringae (causing shoot blight on apple trees). The detection limit of DS-LAMP was 10 cells per LAMP reaction, equivalent to $10^4$ cells per ml of the sample extract. DS-LAMP successfully diagnosed the pathogens on four fire-blight infected apple and pear orchards. In addition, it could distinguish black shoot blight from fire blight. The $B{\ddot{u}}hlmann$-LAMP, developed previously for on-site diagnosis of fire blight, did not give consistent results for specificity to E. amylovora and on-site diagnosis; it gave positive reactions to three strains of E. pyrifoliae and two strains of P. syringae pv. syringae. It also, gave positive reactions to some healthy sample extracts. DS-LAMP, developed in this study, would give more accurate on-site diagnosis of fire blight, especially in the Republic of Korea, where fire blight and black shoot blight coexist.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.10a
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• pp.291-293
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• 2022

The preparation of dendritic molecule for photodynamic diagnosis (PDD) or photodynamic therapy (PDT) has been interested on design and synthesis of macromolecule toward a new generation. Herein, the binding site of polyether group is an important role on the construction of macromolecule toward a new generation. Therefore, we will be presented on the preparation of dendritic molecule having the binding site.

• Proceedings of the KIEE Conference
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• 2004.05b
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• pp.97-100
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• 2004

HFPD(High Frequency Partial Discharge) measurement has been widely performed to diagnosis of cable system in the world although Korea just begin. The efficiency of measurement has been reported and proved by many researchers who are engaged in the diagnosis of high power apparatus. LG Cable has investigated the technology of diagnosis for cable system by means of PD measurement for 10 years. In this paper, PD measurement with portable device was performed to evaluate the quality of the 154kV transmission line and the 22.9kV XLPE cable line. Test results are shown that the HFPD detection technology is very attractive for the diagnosis of power cable system by high detection sensitivity at on-site.

• Journal of Biosystems Engineering
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• v.24 no.3
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• pp.267-274
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• 1999

An internet and network based software and related interface have been developed, which can remotely control and manage an on-site operating system. Developed software modules were composed of two parts: monitoring/management modules and control/diagnosis modules were developed for the network status, warehouse, production and selling status. Modules of control with diagnosis were developed for the on-site operating system and interface. Each module was integrated and the whole modules have been tested with an automatic mushroom grading/sorting system which was built in a laboratory. Developed software modules worked successfully without any uncommon situations such as system down caused by the software or data transfer error. Each software module was developed independently in order to apply easily to other existing on-site systems such as rice processing centers, fruit and vegetable sorting, packaging and distribution centers scattered over the country.

• Research in Plant Disease
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• v.23 no.4
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• pp.306-313
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• 2017

Recently fire blight occurred in the Republic of Korea and eradication program for the disease has been executed since then. Specificity and detection sensitivity of the 2 antibody-based diagnostic strips to Korean isolates of Erwinia amylovora (Ea) and their application for on-site diagnosis were evaluated in this study. Ea AgriStrip, a commercial diagnostic kit, and EB strip, developed in this study, reacted positively to the all tested Korean Ea strains and also to most of Erwinia pyrifoliae (Ep) strains causing black shoot blight. They reacted negatively to all Pusedomonas syringae pv. syringae (Pss) strains that cause shoot blight on apple. Detection sensitivity was similar between the 2 strips. For on-site diagnosis, the two strips reacted positively only to the extractions of the fire-blighted samples on all fire blight occurred orchards except one orchard at which on-site diagnosis was carried out at winter time. In addition, they reacted positively to the black-shoot blighted extractions from the black shoot blight occurred apple orchard. These results suggest that both EB strip and Ea AgriStrip would be useful for on-site diagnosis of fire blight in Korea.

• Research in Plant Disease
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• v.20 no.1
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• pp.15-20
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• 2014

A rapid, user-friendly and simple immune-chromatographic dipstick kit named 'rapid immune-gold strip' (RIGS) kit was developed in a novel single strip format to detect on-site detection of Tomato spotted wilt virus (TSWV). Immunoglobulin G (IgG) from polyclonal antisera raised in rabbits against TSWV was purified through protein-A affinity chromatography and then the purified TSWV-IgG was conjugated to colloidal gold nano-particles which served as a test line on nitrocellulose membrane. Protein A that non-specifically binds to TSWV antibody was used as a control line on the same strip. The diagnosis process with the TSWV-RIGS involves simply grinding the suspect plant sample in a bag that contains the extraction buffer and inserting the strip the bag. Results can be seen in 2-5 minutes. The flow of the complexes of gold particles coated with TSWV-IgG and a crude sap from TSWV-infected pepper, tobacco and tomato plants resulted in intensive color formed on the test lines proportional to the concentrations of TSWV. The RIGS-TSWV kit did not show any cross-reactions against other tomato-infecting viruses unrelated to TSWV. These results indicate that the TSWV-RIGS kit is highly sensitive and is not required for laboratory training and experience prior to testing. The TSWV-RIGS kit is suitable for on-site detection of suspect TSWV-infected plants as well as for laboratory diagnosis.

• Journal of Oral Medicine and Pain
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• v.20 no.2
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• pp.477-487
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• 1995

The purpose of this study was to assess the thermographic differences of craniomandibular area between normal individual and patients with craniomandibular disorders and to compare the differences between clinical and thermographical assessment. The author had used 50 subjects as materials for this study, which was divided into 2 groups (first group included 15 healthy subjects and second group included 35 patients) with craiomandibular disorders; 17 subjects had normal disc-condyle relationship, 13 subjects had disc displacement with reduction and 5 subjects had disc displacement without reduction. Agema 870 thermovision(D.I.T.I.) was used to take thermographs with $0.1^{\circ}C$ difference of gradual temperature shift. The results were as follows : 1. Of 34 patients with craniofacial pain, 15(44%) subjects showed hyperthermia on the pain site in the thermography, 8(23.5%)exhibited hyperthermia on the site opposed to the pain site, and 11(32.4%) did not show any significant thermal change. One patient without craniofacial pain showed hyperthermia on the site opposed to the site of disc displacement without reductin. 2. Of 35 patients with craniofacial pain or disc displacement, 24(68.6%) subjects showed a significant thermal difference between symptomatic and asymptomatic sides of the face, but 11(31.4%) did not show any difference. 3. Of 17 patients with pain but with normal disc-condyle relationship, 8(47.1 subjects showed hyperthermia on the pain site, 4(23.5%) showed on the site opposed to the pain site, and 5(29.4%) did not show any significant thermal change. 4. Or 13 patients with pain and disc displacement with reduction, 6(46.2%) subjects showed hyperthermia on the pain site, 3(23.1%) showed on the site opposed to the pain site, and 4(30.8%) did not show any significant thermal change. 5. 15 healthy subjects did not show any thermal differences between the both sides of the face.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.5
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• pp.553-560
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• 2003

On-site diagnosis of chiller performance is an essential step fur energy saving business. The main purpose of the on-site diagnosis is to predict the COP of a target chiller. Many models based on thermodynamics background have been proposed for this purpose. However, they have to be modified from chiller to chiller and require deep insight into thermodynamics that most of field engineers are often lacking in. This study focuses on developing an easy-to-use diagnostic technique that is based on adaptive neuro-fuzzy inference system (ANFIS). Quality of the training data for ANFIS, sampled over June through September, is assessed by checking COP prediction errors. The architecture of the ANFIS, its error bounds, and collection of training data are described in detail.

• Journal of Korean Medical classics
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• v.23 no.1
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• pp.317-319
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• 2010

The collateral diagnosis method is unique in Traditional Chinese Medicine diagnosis methods which has important clinical value. In my article, the contents of the "Hwangjenaegyeong(黃帝內經)" related to this method is discussed. According to the site of inspection in the diagnosis process, there are five types as following: inspection of the face and surface, the thenar, the orifices, abdominal collaterals and finally the index finger of children. This method can inspire clinical practitioners.

• International journal of advanced smart convergence
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• v.10 no.4
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• pp.22-29
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• 2021

For web application vulnerability diagnosis, from the development stage to the operation stage, it is possible to stably operate the web only when there is a policy that is commonly applied to each task through diagnosis of vulnerabilities, removal of vulnerabilities, and rapid recovery from web page damage. KISA presents 28 evaluation items for technical vulnerability analysis of major information and communication infrastructure. In this paper, we diagnose the vulnerabilities in the automobile goods shopping mall website and suggest security measures according to the vulnerabilities. As a result of diagnosing 28 items, major vulnerabilities were found in three items: cross-site scripting, cross-site request tampering, and insufficient session expiration. Cookie values were exposed on the bulletin board, and personal information was exposed in the parameter values related to passwords when personal information was edited. Also, since the session end time is not set, it was confirmed that session reuse is always possible. By suggesting security measures according to these vulnerabilities, the discovered security threats were eliminated, and it was possible to prevent breaches in web applications and secure the stability of web services.