• Biomedical Science Letters
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• v.13 no.4
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• pp.251-261
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• 2007

A myofiber of skeletal muscle is composed of myofibrils, sarcolemma (plasma membrane), and constameres, which anchor the myofibrils to the sarcolemma. Achvillin is a recently identified F-actin binding muscle protein, co-isolates with dystrophin and caveolin-3 in low-density sarcolemma of striated muscle, and colocalizes with dystrophin at costameres, the specialized adhesion sites in muscle. Archvillin also binds to nebulin and localizes at myofibrillar Z-discs, the lateral boundaries of the sarcomere in muscle. However other roles of archvillin on the dynamics of myofibrillogenesis remain to be defined. The goal of this study is, by using siRNA-mediated gene silencing technique, to investigate the effect of archvillin on the dynamics of myofibrillogenesis in cell culture of a mouse skeletal myogenic cell line (C2C12), where presumptive myoblasts withdraw from the cell cycle, fuse, undergo de novo myofibrillogenesis, and differentiate into mature myotubes. The roles of archvillin in the assembly and maintenance of myofibril and during the progression of myofibrillogenesis induced in skeletal myoblast following gene silencing in the cell culture were investigated. Fluorescence microscopy demonstrated that the distribution of archvillin was changed along the course of myofibril assembly with nebulin, vinculin and F-actin and then located at Z-lines with nebulin. Fluorescence microscopy demonstrated that knockdown of mouse archvillin expression led to an impaired assembly of new myofibrillar clusters and delayed fusion and myofibrillogenesis although the mouse archvillin siRNA did not affect those expressions of archvillin binding proteins, such as nebulin and F-actin. This result is corresponded with that of RT-PCR and western blots. When the perturbed archvillin was rescued by co-transfection with GFP or Red tagged human archvillin construct, the inhibited cell fusion and myotube formation was recovered. By using siRNA technique, archvillin was found to be involved in early stage of myofibrillogenesis. Therefore, the current data suggest the idea that archvillin plays critical roles on cell fusion and dynamic myofibril assembly.

• The korean journal of orthodontics
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• v.40 no.5
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• pp.325-333
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• 2010

Objective: The aim of this study was to illustrate the circumferential comfortable retainer (CCR) as a removable maxillary retainer with good potential patient compliance and to evaluate the discomfort of the retainers including distorted speech, gagging sensation and appliance discomfort. Methods: Sixty-six orthodontic patients (male, 23; female, 43; mean age, $23.42{\pm}10.19$ years) who received orthodontic treatment with fixed orthodontic appliances were randomly assigned to two groups after debonding, a conventional wraparound retainer (CWR) group that fully covers the palate with an acrylic plate and a highly polished surface, and a circumferential comfortable retainer (CCR) group which has a horseshoe shaped base plate with three folds on the anterior region. A questionnaire that had a visual analog scale (VAS) which consists of a 100-mm horizontal line with 2 end-points labeled "no discomfort" on the left and "worst discomfort" on the right, with regard to distorted speech, gagging sensation and discomfort, was administered to patients after 4 weeks of retainer wear. The Mann-Whitney test was used to test the hypothesis that there was no difference between the two retainers. Results: Comparing distorted speech and discomfort, the CCR group significantly had lower values than the CWR group ($p$ < 0.05). Comparing gagging sensation, the CCR group had lower values than the CWR group but there were no statistically significant differences between groups ($p$ = 0.146). Conclusions: In conclusion, the results suggest that the circumferential comfortable retainer (CCR) might facilitate patient compliance and thereby improve the maintenance of the fixed orthodontic treatment outcome.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.14 no.4
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• pp.143-160
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• 2019

The domestic pharmacy market has undergone a lot of changes starting from the division of medicine. Along with the division of medicine, patients who had previously visited the pharmacy were moved to a hospital or clinic. As the pharmacy became more dependent on the illness and the clinic, the pharmacy began various activities to search for ways other than prescription drugs. At this time, the importance of distribution was emphasized around the time, and as the need to strengthen the competitiveness of pharmacies increased, they rapidly grew into the franchise market. Pharmacy franchise companies continue to lecture on academic management to strengthen the expertise of pharmacists' functions in line with the pharmacy market, which is different from ordinary franchise, in addition to private brand products for pharmacies, diversifying pharmacy handling items, And strengthening its market competitiveness. but research to support it are insufficient. As a research to help this, we analyzed factors affecting the intention of re-contracting and recommendation intention that affect the maintenance and expansion size of the drugstore franchise market. As a result showed the intention of re-contracting and the intention of recommending are affected by positive influence in brand trust. In addition, Brand Promise, Employee authenticity, Originality, Product excellence, Brand reputation, Brand identification, Educational Satisfaction were found to affect brand trust.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.27 no.4
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• pp.314-320
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• 2001

Matrix metalloproteinases have long been viewed as ideal candidates for proteinases that enables tumor cells to permeated basement membrane defenses and invade surrounding tissue. There is growing evidence that the MMPs have an expanded role, as they are important for the creation and maintenance of a microenvironment that facilitates growth and angiogenesis of tumors at primary and metastatic sites. MT-MMPs are not secreted but instead remaining attached to cell surfaces. Although not all of the MT-MMPs are fully characterized, MT-MMPs have important role in localizing and activating secreted MMPs. The MMP genes are transcriptionally responsive to a wide variety of oncogene, growth factors, cytokine, and hormones. Currently, a number of MMP inhibitors are being developed and some have reached clinical trials as anti-metastatic or anti-cancer therapies. MT1-MMP is involved in the activation of proMMP-2. MT1-MMP is significant not only as a tumor marker but as a new target for chemotherapy against cancer. The purpose of this study was to evaluate the effects of protein kinase C inhibitor(genistein) on the proliferation of HT1080 and expression of MT1-MMP mRNA. Human fibrosarcoma cell line HT1080 was cultured and divided 2 groups. The experimental group was treated with $100{\mu}M$ genistein and incubated 12h, 24h for $[3^H]-thymidine$ uptake assay and northern hybridization individually. And the control group was treated with same amount of PBS for the above procedures. $[3^H]-thymidine$ incorporation was measured with ${\beta}$ ray detector. And RT-PCR and northern blotting for MT1-MMP mRNA was performed. The results were as follows 1. $[3^H]-thymidine$ uptake was reduced in experimental group with statistical significance. 2. MT1-MMP mRNA expression was significantly reduced in experimental group. These results showed that protein kinase C inhibitor (genistein) inhibited proliferation of HT1080 and almost completely blocked transcription of MT1-MMP mRNA. So, it is possible to use the protein kinase inhibitor (genistein) as anti-metastatic and anti-proliferative agent.

• The KIPS Transactions:PartA
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• v.13A no.1 s.98
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• pp.45-52
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• 2006

To perform on-line maintenance for Distributed Information System, it is indispensable to disseminate files to participant nodes in the network. When users' requests for file deployment occur simultaneously in a short period a deployment server falls into overload phase, which is often called Flash Crowds. h common solution to avoid Flash Crowds is to increase hardware capacity. In this paper, we propose a software solution based on P2P, which does not cost any additional expense. In the proposed solution, nodes in the network are grouped into subnetworks one of which is composed of only neighboring nodes. In each subnetwork, copies of deployment files can be transferred to each other. Consequently, it brings about the effect of load balancing in deployment server. To raise the effectiveness, target files for deployment are packed into one package. Before being transferred, each package is divided into multiple equal-sized segments. A deployment server in a normal phase transmits a package requested from nodes in segment units. However a deployment server is overloaded, if segments already exist in the subnetwork participant nodes in the subnetwork receive necessary segments from neighboring nodes. In this paper, we propose data structures and algorithm for this approach and show performance improvement through simulation.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.12
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• pp.778-784
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• 2017

In this paper, we propose wired and wireless distributed control systems designed to improve the freight logistics efficiency and verify wired distributed control systems. The verification condition required that 50 cargo vehicles be connected and operated to travel 21 km from Busan Sinhang station to Jinlye Station at an average speed of about 100km/h. The verification results show that the traction output and braking output of the control and controlled cars are dispersed by the wired distributed control system. The application is expected to more than double the efficiency of the logistics compared to the existing freight transportation system. However, in the case of the wired distributed control system, cable installation and maintenance are difficult, and it is impossible to change the combination of freight vehicles. Through the verification of the wired distributed control system, the applicability of distributed control systems to freight vehicles in Korea was confirmed and the system was further developed to produce a wireless distributed control system. In order to apply the wireless distributed control system, a propagation environment analysis for the ISM band was performed in the testbed and, as a result, it was confirmed that Wifi technology using the ISM band could be utilized. In order to use the WDP (Wireless Distributed Power) devices newly installed in the target vehicles, the transmission / reception control signals associated with the propulsion / braking / total control devices are defined. In the case of wireless distributed control systems, the convenience of their application and operation is guaranteed, but reliability and emergency safety measures should because of the dependence of the control of the vehicle on radio signals.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.38 no.6
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• pp.106-112
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• 2001

Unfortunately, the area without economical efficiency, especially the far distance sea, is much lower than that of a urban area-built-up area. It should be promoted the equivalent level to a urban area in the light of future-oriented universal service. Actually, Because propagation environment of mobile communication in the sea is greatly different from that for inland focused on built-up area, a propagation prediction model in the sea should be distinguished from inland-based one. Accordingly, the purpose of this study is to suggest the propagation prediction model for the sea service as a method to minimize unnecessary facilities investment and maintenance caused by additional or new building of a base station. If mobile phone service for far distance sea is provided by expanding limited communication zone of narrow band CDMA mobile communication whose spread band FA is 1.2288MHz. Suggested propagation prediction model includes five parameters to minimize facilities investment of a base station and maximize channel capacity: equivalent line of sight, chip delay by PN code, antenna altitude, power of base station and gain of antennas. Finally, suggested propagation prediction model is simulated and, the results are examined for its utility by comparing with loss of free space.

• Journal of the Korean Society for Railway
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• v.19 no.2
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• pp.262-269
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• 2016

Subway station scales are determined by peak predictions. In this study, the purpose behind the installation of a subway is public transportation convenience and public interest, but economic validity is also important. By proving that the scale of the station is excessive with regard to the target station size for Seoul subway Line 5-8, a reasonable plan. can be sought. According to station installation standards, the area of the station under investigation here is out of the service levels by six stages (A~F), and it must be four or more levels (D). The Actual level for the B level is a two-step design. The Actual ratio for over- Peak predictions is only 17.8% on average. The results of measurements of the excess area and determination of the excessive costs were analyzed by subdividing the area and by calculating it based on the B level, finding that it is possible to provide benefits for customers only in the current design, with an area ratio of 16.3%. Given the weight, it was estimated that current conditions can meet the needs of only 18.6% of the current area. Simplifying the scale calculation method of the station, it is convenient, safe, and advantageous to move citizens only if the scale can be streamlined. Then, with a reduced initial investment, maintenance costs during the operation can be reduced.

• Korean Journal of Food Science and Technology
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• v.53 no.6
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• pp.715-721
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• 2021

Cognitive impairment and Alzheimer's disease are serious social problems associated with the rising elderly population in Korea. 1,2,3,4,6-Penta-O-galloyl-β-ᴅ-glucopyranose (PGG) is a gallotannin isolated from medicinal plants such as Rhus chinensis. This study was performed to evaluate the effect of PGG on biomarkers related to cognitive function in human neuroblastoma SK-N-SH cells. Inhibition of acetylcholinesterase (AChE) activity is considered to be one of the main therapeutic strategies. PGG inhibited AChE activity in the test tube as well as in SK-N-SH cells. In addition, PGG induced protein and mRNA expression of brain-derived neurotrophic factor (BDNF), which is a mammalian neurotrophin that plays major roles in the development, maintenance, repair, and survival of neuronal populations. As one of the underlying molecular mechanisms that induce BDNF expression, PGG induced the activation of Ca²⁺/calmodulin (CaM)-dependent protein kinase II (CaMKII)-cAMP response element binding protein (CREB) pathway. In conclusion, PGG may be an useful material for improving cognitive function.

• The Korean Journal of Air & Space Law and Policy
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• v.33 no.2
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• pp.115-168
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• 2018

An amendment to Japanese Civil Aeronautics Act came into effect December 10, 2015. The Act prohibits flying drones over residential areas or areas surrounding an airport without permission from the Minister of Land, Infrastructure and Transportation. Flying drones during night time and during an event is also prohibited. The term "UAV" or "UA" means any aeroplane, rotorcraft, glider or airship which cannot accommodate any person on board and can be remotely or automatically piloted (Excluding those lighter than a certain weight (200 grams). Any person who intends to operate a UAV is required to follow the operational conditions listed below, unless approved by the Minister of Land, Infrastructure, Transport and Tourism; (i) Operation of UAVs in the daytime, (ii) Operation of UAVs within Visual Line of Sight (VLOS), (iii) Maintenance of a certain operating distance between UAVs and persons or properties on the ground/water surface, (iv) Do not operate UAVs over event sites where many people gather, (v) Do not transport hazardous materials such as explosives by UAV, (vi) Do not drop any objects from UAVs. Requirements stated in "Airspace in which Flights are Prohibited" and "Operational Limitations" are not applied to flights for search and rescue operations by public organizations in case of accidents and disasters. This paper analyzes some issues as to regulations of UAVs in Korean Aviation Safety Act by comparing the regulations of UAVs in Japanese Civil Aeronautics Act. This paper, also, offers some implications and suggestions for regulations of UAVs under Korean Aviation Safety Act.