• 한국응용과학기술학회지
• /
• 제35권4호
• /
• pp.1407-1412
• /
• 2018

본 연구는 오존화 올리브오일이 독성이 적으면서 다양한 종류의 미생물을 치사시킬 수 있으나 경우투여나 복강투여에도 인체에 해가 없는 것으로 알려져 있다. 그리고 국내에서는 아직 동물에 대한 안정성 실험이 없었다. 그래서 본 연구에서는 오존화 올리브오일을 사용하여 동물의 안구를 통한 안정성 실험을 하였다. 구체적인 연구에서는 rabbit에서 오존화오일(고농도)의 동물 눈 안점막 자극을 평가하기 위해 실시하였고, 시험물질 처치부위를 관찰한 결과, 비세척군 및 세척군 모두에서 안점막 자극이 관찰되지 않았다.

• Fisheries and Aquatic Sciences
• /
• 제24권8호
• /
• pp.277-283
• /
• 2021

Kudoa septempunctata has been reported as a new parasite in aquacultured olive flounder Paralichthys olivaceus, and also as a causative agent of food poisoning in humans. This paper investigated the infection of K. septempunctata in 216 sashimi and 20 sushi made of olive flounders in Busan, Korea. Among 236 samples, K. septempunctata was detected in eleven sashimi with 6-7 polar capsules by the microscopy. Among eleven sashimi, five sashimi were positive in Polymerase Chain Reaction (PCR) assay with the targets of 18S rDNA and 28S rDNA. The genotype of all the five PCR results is identified as the genotype ST3 which is common in Korea. K. septempunctata was found in olive flounders sashimi from Samcheonpo and Wando outside of Jeju Island. These findings would contribute to establish the standard of K. septempunctata for preventing food-borne outbreaks in advance and providing further preventive management for the seafood safety.

• 한국수산과학회지
• /
• 제56권5호
• /
• pp.716-720
• /
• 2023

This study was designed to analyze the effects of reducing water turnover in olive flounder Paralichthys olivaceus farms, focusing on olive flounder growth, decreasing electricity costs, and developing measures to ensure business stability. Daily water turnover was set at 18 in the control group and six in the experimental group. Juvenile fish were reared for 12 months. No significant differences in mean weight were observed between groups until five months. After five months, the mean weight of the control group grew significantly faster than that of the experimental group. Maintaining water turnovers leverl at six turnovers for the first five months after stocking juvenile fish and then increasing water turnover resulted in a 34.4% reduction in electricity costs compared to the control group. This approach presents a potential method to enhance the profitability of olive flounder farms and ensure stable productivity and profitability without sacrificing olive flounder growth.

• 한국어병학회지
• /
• 제37권1호
• /
• pp.123-132
• /
• 2024

Viral diseases cause enormous economic losses to the olive flounder (Paralichthys olivaceus) aquaculture industry in Korea. This study aimed to identify immune-related genes expressed in the kidney of olive flounder injected with Polyinosinic-polycytidylic acid (Poly (I:C)). Thirty fish were divided into two groups by intraperitoneal injection of 100µl of diethylpyrocarbonate-treated water or poly I:C per fish. Kidney tissues at day 3 and 30 after the injection were used for RNA-seq analysis to identify differentially expressed genes (DEGs). Poly I:C group upregulated il8, cfh, tnfaip2b, c3b.2, ly6d and cd38 genes at 3 days post-injection. Additionally, cd22, ccl34a.3, c9, cxcl19, ccl27a, ccl7, and cfh genes were upregulated at 30 days post-injection. Differential expression gene analysis showed that poly I:C has both short and long-term immune effects in olive flounder. This study provides a theoretical basis for understanding the molecular mechanism of the short and long-term immune effects of poly I:C.

• 한국식품영양과학회지
• /
• 제34권4호
• /
• pp.503-508
• /
• 2005

본 연구는 올리브 잎과 월계수 잎의 화학적 특성들을 분석하고, 비교하여 식품 소재화나 가공시 활용될 수 있는 기초자료를 제공하고자 하였다. 올리브 잎과 월계수 잎의 수분함량은 각각 $3.95,\;8.50\%$이었다. 올리브 잎은 조단백질 $11.04\%$, 조지방 $7.45\%$, 조회분 $5.05\%$, 탄수화물 $76.46\%$이었고, 월계수 잎은 조단백질 $7.23\%$, 조지방 $7.21\%$, 조회분 $3.72\%$, 탄수화물 $81.84\%$이었다. 올리브 잎의 주요 아미노산은 glutamic acid$(1086.8\;mg\%)$와 aspartic acid$(918.8\;mg\%)$이었고, 월계수 잎은 glutamic acid$(621.2\;mg\%)$와 leucine$(558.6\;mg\%)$이 가장 많이 함유된 아미노산이었다. 올리브 잎의 주요 유리당은 sucrose$(1.55\%)$이었고, linolenic acid$(33.0\%)$, palmitic acid$(31.1\%)$가 조지방 중의 가장 많이 함유된 지방산이었다. 월계수 잎에는 glucose$(1.54\%)$와 fructose$(0.99\%)$가 주요 유리당이었고, palmitic acid$(35.2\%)$와 linolenic acid$(17.8\%)$가 조지방중의 가장 많이 함유된 지방산이었다. 무기질 함량은 올리브 잎과 월계수 잎 모두에서 Ca이 $929.6\;mg\%,\;836.2\;mg\%$로 가장 많이 함유되어 있었다. 비타민 A는 올리브 잎 5.10 mg/100 g, 월계수 잎 6.49 mg/100 g로 나타났고 비타민 C는 올리브 잎 36.64 mg/100 g, 월계수 잎 13.86 mg/100 g로 나타났다. 올리브 잎과 월계수 잎 모두에서 비타민 $B_1,\;B_2$, niacin이 소량 함유되어 있었으며 $B_6$과 $B_{12}$은 검출되지 않았다.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2008년도 학술대회 논문집 정보 및 제어부문
• /
• pp.441-442
• /
• 2008

본 논문에서는 비선형 시스템인 척추동물의 Inferior Olive 뉴론을 대상으로 center manifold와 normal form 해를 통하여 bifurcation해석을 한다. IO 모델에 고정점이 있음을 보이고, 3차 항까지 근사를 하며 행렬 기저벡터를 통하여 해를 구하는 과정을 제시한다.

• 한국정보처리학회:학술대회논문집
• /
• 한국정보처리학회 2017년도 추계학술발표대회
• /
• pp.464-465
• /
• 2017

본 논문에서는 애자일 방법론과 스크럼 기법에 대해서 소개하고 국내 SI프로젝트에 스크럼 기법을 적용하기 어려운 이유와 스크럼이 적용된 SI프로젝트를 성공적으로 완료하기 위한 가이드를 제안한다.

• 한국어병학회지
• /
• 제17권3호
• /
• pp.217-222
• /
• 2004

This study was performed to investigate the immunological side effects of chloramphenicol (CAP) on olive flounder, Paralichthys olivaceus. To investigate immunological effects on olive flounder, we determined the changes of chemiluminescence (CL) response of flounder kidney-derived leucocyte after the treatment of CAP in vivo and in vitro. The CL activity was significantly decreased in a dose-dependent manner during the treatment of CAP in vitro. Similarly, a dose-dependent reduction of CL response, although not significant, were observed during the treatment of CAP in vivo. The results suggest that CAP reduced the function of flounder phagocytosis in vivo and in vitro, indicating the immunosuppressive ability of CAP.

• 한국어병학회지
• /
• 제16권3호
• /
• pp.147-152
• /
• 2003

The CMV promoter driven luciferase reporter gene coding plasmid (pcDNA-luc) was constructed and used as a model for DNA immunization study. Expression of the recombinant luciferase protein was confirmed in vitro in RTG-2 cell line before using in vivo study in olive flounder. In dose response study, the maximum expression of the luciferase gene was found in the group injected with 10-15μg of plasmid DNA. The kinetic study showed that the luciferase gene expression was reached at the maximum level at one day after injection and slightly decreased after then but significantly high level of expression was sustained until the conducted experiment of 7 days. In the study of tissue distribution of gene expression, it was found that luciferase gene was expressed at the significant level in immune organs such as gill and spleen, located far from the injected site, suggesting the systemic distribution of the intramuscularly injected DNA in olive flounder.

• Fisheries and Aquatic Sciences
• /
• 제6권2호
• /
• pp.101-104
• /
• 2003

A cDNA clone for the olive flounder (Paralichthys olivaceus) transferrin receptor (fTfR) was isolated from a leukocytes cDNA library. The fTfR gene consisted of 2,319 bp encoding 773 amino acid residues. The amino acid sequence alignment of the fTfR showed that their size and hydrophobic profile are similar. In addition, the Tyr-Thr-Arg-Phe (YTRF) motif that is the recognition signal for high-efficiency endocytosis, is conserved very well. This motif is important for functional properties of TfR. The deduced amino acid sequence had $42.4-42.9\%$ identities with the previously reported TfRs of vertebrates. The fTfR was expressed in the blood, kidney, spleen, and liver of healthy olive flounder by the Northern blot hybridization.