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A study on Kudoa septempunctata infection from sashimi and sushi of olive flounder Paralichthys olivaceus in Busan, South Korea

  • Koo, Hee-soo (Foodborne Diseases Inspection Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Park, Ji-young (Foodborne Diseases Inspection Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Sung, Gyung-hye (Foodborne Diseases Inspection Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Park, Eun-hee (Foodborne Diseases Inspection Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Ku, Pyeong-tae (Foodborne Diseases Inspection Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Lee, Mi-ok (Foodborne Diseases Inspection Team, Busan Metropolitan City Institute of Health & Environment)
  • Received : 2021.05.11
  • Accepted : 2021.07.21
  • Published : 2021.08.31

Abstract

Kudoa septempunctata has been reported as a new parasite in aquacultured olive flounder Paralichthys olivaceus, and also as a causative agent of food poisoning in humans. This paper investigated the infection of K. septempunctata in 216 sashimi and 20 sushi made of olive flounders in Busan, Korea. Among 236 samples, K. septempunctata was detected in eleven sashimi with 6-7 polar capsules by the microscopy. Among eleven sashimi, five sashimi were positive in Polymerase Chain Reaction (PCR) assay with the targets of 18S rDNA and 28S rDNA. The genotype of all the five PCR results is identified as the genotype ST3 which is common in Korea. K. septempunctata was found in olive flounders sashimi from Samcheonpo and Wando outside of Jeju Island. These findings would contribute to establish the standard of K. septempunctata for preventing food-borne outbreaks in advance and providing further preventive management for the seafood safety.

Keywords

References

  1. Ahn M, Woo H, Kang B, Jang Y, Shin T. Effect of oral administration of Kudoa septempunctata genotype ST3 in adult BALB/c mice. Parasite. 2015;22:35. https://doi.org/10.1051/parasite/2015035
  2. Grabner DS, Yokoyama H, Shirakashi S, Kinami R. Diagnostic PCR assays to detect and differentiate Kudoa septempunctata, K. thyrsites and K. lateolabracis (Myxozoa, Multivalvulida) in muscle tissue of olive flounder (Paralichthys olivaceus). Aquaculture. 2012;338-341:36-40. https://doi.org/10.1016/j.aquaculture.2012.01.022
  3. Harada T, Kawai T, Sato H, Yokoyama H, Kumeda Y. Development of a quantitative polymerase chain reaction assay for detection of Kudoa septempunctata in olive flounder (Paralichthys olivaceus). Int J Food Microbiol. 2012;156:161-7. https://doi.org/10.1016/j.ijfoodmicro.2012.03.018
  4. Jang Y, Ahn M, Bang H, Kang B. Effects of Kudoa septempunctata genotype ST3 isolate from Korea on ddY suckling mice. Parasite. 2016;23:18. https://doi.org/10.1051/parasite/2016020
  5. Jeon CH, Kim JH. Detection of Kudoa septempunctata (Myxozoa: Multivalvulida) in olive flounder (Paralichthys olivaceus) by DNA probe-based melting curve analysis. In: Proceedings of the 2016 KOFFST International Conference; 2016; Busan, Korea. p. 376.
  6. Kawai T, Sekizuka T, Yahata Y, Kuroda M, Kumeda Y, Iijima Y, et al. Identification of Kudoa septempunctata as the causative agent of novel food poisoning outbreaks in Japan by consumption of Paralichthys olivaceus in raw fish. Clin Infect Dis. 2012;54:1046-52. https://doi.org/10.1093/cid/cir1040
  7. Kim JJ, Ryu S, Lee H. Foodborne illness outbreaks in Gyeonggi province, Korea, following seafood consumption potentially caused by Kudoa septempunctata between 2015 and 2016. Osong Public Health Res Perspect. 2018;9:66-72. https://doi.org/10.24171/j.phrp.2018.9.2.05
  8. Kim WS, Kong KH, Jung SJ, Jung MH, Jeon CH, Kim JH, et al. A survey of Kudoa septempunctata in olive flounder (Paralichthys olivaceus) hatcheries in the southwestern coast of Korea between 2014 and 2015. J Fish Pathol. 2015;28:109-12. https://doi.org/10.7847/JFP.2015.28.2.109
  9. Matsukane Y, Sato H, Tanaka S, Kamata Y, Sugita-Konishi Y. Kudoa septempunctata n. sp. (Myxosporea: Multivalvulida) from an aquacultured olive flounder (Paralichthys olivaceus) imported from Korea. Parasitol Res. 2010;107:865-72. https://doi.org/10.1007/s00436-010-1941-8
  10. Ohnishi T, Kikuchi Y, Furusawa H, Kamata Y, Sugita-Konishi Y. Kudoa septempunctata invasion increases the permeability of human intestinal epithelial monolayer. Foodborne Pathog Dis. 2013;10:137-42. https://doi.org/10.1089/fpd.2012.1294
  11. Song JY, Choi JH, Choi HS, Jung SH, Park MA. Monitoring of Kudoa septempunctata in cultured olive flounder and wild fish in Jeju Island during 2012. J Fish Pathol. 2013;26:129-37. https://doi.org/10.7847/JFP.2013.26.3.129
  12. Song JY, Kim MJ, Choi HS, Jung SH. Monitoring Kudoa septempunctata in cultured olive flounder Paralichthys olivaceus in different regions of Korea in 2013. Korean J Fish Aquat Sci. 2014 ;47:611-21. https://doi.org/10.5657/KFAS.2014.0611
  13. Takeuchi F, Ogasawara Y, Kato K, Sekizuka T, Nozaki T, Sugita-Konishi Y, et al. Genetic variants of Kudoa septempunctata (Myxozoa: Multivalvulida), a flounder parasite causing foodborne disease. J Fish Dis. 2016;39:667-72. https://doi.org/10.1111/jfd.12395