• Journal of the Korean Applied Science and Technology
• /
• v.35 no.4
• /
• pp.1407-1412
• /
• 2018

This study suggests that ozonated olive oil can kill a wide variety of microorganisms while being less toxic but in the case of administration or abdominal administration, the human body is harmless. In Korea, there is no animal stability test yet. In this study, ozonated olive oil was used to test the stability of the animal through the eye. Specific experiments were performed to evaluate animal Ocular irritation safe test of ozonized oil (high concentration) in rabbits. and Observation of the treatment point on the test substance, ozonated olive oil showed no Ocular irritation in both the non-washing group and the washing group.

• Fisheries and Aquatic Sciences
• /
• v.24 no.8
• /
• pp.277-283
• /
• 2021

Kudoa septempunctata has been reported as a new parasite in aquacultured olive flounder Paralichthys olivaceus, and also as a causative agent of food poisoning in humans. This paper investigated the infection of K. septempunctata in 216 sashimi and 20 sushi made of olive flounders in Busan, Korea. Among 236 samples, K. septempunctata was detected in eleven sashimi with 6-7 polar capsules by the microscopy. Among eleven sashimi, five sashimi were positive in Polymerase Chain Reaction (PCR) assay with the targets of 18S rDNA and 28S rDNA. The genotype of all the five PCR results is identified as the genotype ST3 which is common in Korea. K. septempunctata was found in olive flounders sashimi from Samcheonpo and Wando outside of Jeju Island. These findings would contribute to establish the standard of K. septempunctata for preventing food-borne outbreaks in advance and providing further preventive management for the seafood safety.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.56 no.5
• /
• pp.716-720
• /
• 2023

This study was designed to analyze the effects of reducing water turnover in olive flounder Paralichthys olivaceus farms, focusing on olive flounder growth, decreasing electricity costs, and developing measures to ensure business stability. Daily water turnover was set at 18 in the control group and six in the experimental group. Juvenile fish were reared for 12 months. No significant differences in mean weight were observed between groups until five months. After five months, the mean weight of the control group grew significantly faster than that of the experimental group. Maintaining water turnovers leverl at six turnovers for the first five months after stocking juvenile fish and then increasing water turnover resulted in a 34.4% reduction in electricity costs compared to the control group. This approach presents a potential method to enhance the profitability of olive flounder farms and ensure stable productivity and profitability without sacrificing olive flounder growth.

• Journal of fish pathology
• /
• v.37 no.1
• /
• pp.123-132
• /
• 2024

Viral diseases cause enormous economic losses to the olive flounder (Paralichthys olivaceus) aquaculture industry in Korea. This study aimed to identify immune-related genes expressed in the kidney of olive flounder injected with Polyinosinic-polycytidylic acid (Poly (I:C)). Thirty fish were divided into two groups by intraperitoneal injection of 100µl of diethylpyrocarbonate-treated water or poly I:C per fish. Kidney tissues at day 3 and 30 after the injection were used for RNA-seq analysis to identify differentially expressed genes (DEGs). Poly I:C group upregulated il8, cfh, tnfaip2b, c3b.2, ly6d and cd38 genes at 3 days post-injection. Additionally, cd22, ccl34a.3, c9, cxcl19, ccl27a, ccl7, and cfh genes were upregulated at 30 days post-injection. Differential expression gene analysis showed that poly I:C has both short and long-term immune effects in olive flounder. This study provides a theoretical basis for understanding the molecular mechanism of the short and long-term immune effects of poly I:C.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.34 no.4
• /
• pp.503-508
• /
• 2005

The chemical properties of olive and bay leaves were investigated and analyzed to provide basic data for food materialization and processing. The moisture contents of olive and bay leaves were $3.95\%$ and $8.50\%$, respectively. The contents of crude protein, crude fat, crude ash and carbohydrates of olive leaf were $11.04\%,\;7.45\%,\;5.05\%\;and\;76.46\%$, respectively. And the contents of same those components of bay leaf were $7.23\%,\;7.21\%,\;3.72\%\;and\;81.84\%$, respectively. Glutamic acid $(1086.8\;mg\%)$and aspartic acid $(918.8\;mg\%)$ in olive leaf were major amino acids, glutamic acid $(621.2\;mg\%)$ and leucine $(558.6\;mg\%)$ in bay leaf were the major amino acids. The major free sugar of olive leaf was sucrose $(1.55\%)$. Whereas major free sugar of bay leaf was glucose $(1.54\%)$. Palmitic acid $(olive\;33.0\%,\;bay\;17.8\%)$ and linolenic acid $(olive\;31.1\%,\;bay\;35.2\%)$ were major fatty acid in crude fat of both olive leaf and bay leaf. The Ca contents were the highest in olive leaf $(929.6\;mg\%)$and bay leaf $(836.2\;mg\%)$. Vitamin A contents of olive and bay leaves were 5.10 mg/100 g and 6.49 mg/100 g, respectively. Vitamin C contents of olive and bay leaves were 36.64 mg/100 g and 13.86 mg/100 g, respectively. But vitamin $B_6$ and $B_{12}$ were not detected.

• Proceedings of the KIEE Conference
• /
• 2008.10b
• /
• pp.441-442
• /
• 2008

본 논문에서는 비선형 시스템인 척추동물의 Inferior Olive 뉴론을 대상으로 center manifold와 normal form 해를 통하여 bifurcation해석을 한다. IO 모델에 고정점이 있음을 보이고, 3차 항까지 근사를 하며 행렬 기저벡터를 통하여 해를 구하는 과정을 제시한다.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2017.11a
• /
• pp.464-465
• /
• 2017

본 논문에서는 애자일 방법론과 스크럼 기법에 대해서 소개하고 국내 SI프로젝트에 스크럼 기법을 적용하기 어려운 이유와 스크럼이 적용된 SI프로젝트를 성공적으로 완료하기 위한 가이드를 제안한다.

• Journal of fish pathology
• /
• v.17 no.3
• /
• pp.217-222
• /
• 2004

This study was performed to investigate the immunological side effects of chloramphenicol (CAP) on olive flounder, Paralichthys olivaceus. To investigate immunological effects on olive flounder, we determined the changes of chemiluminescence (CL) response of flounder kidney-derived leucocyte after the treatment of CAP in vivo and in vitro. The CL activity was significantly decreased in a dose-dependent manner during the treatment of CAP in vitro. Similarly, a dose-dependent reduction of CL response, although not significant, were observed during the treatment of CAP in vivo. The results suggest that CAP reduced the function of flounder phagocytosis in vivo and in vitro, indicating the immunosuppressive ability of CAP.

• Journal of fish pathology
• /
• v.16 no.3
• /
• pp.147-152
• /
• 2003

The CMV promoter driven luciferase reporter gene coding plasmid (pcDNA-luc) was constructed and used as a model for DNA immunization study. Expression of the recombinant luciferase protein was confirmed in vitro in RTG-2 cell line before using in vivo study in olive flounder. In dose response study, the maximum expression of the luciferase gene was found in the group injected with 10-15μg of plasmid DNA. The kinetic study showed that the luciferase gene expression was reached at the maximum level at one day after injection and slightly decreased after then but significantly high level of expression was sustained until the conducted experiment of 7 days. In the study of tissue distribution of gene expression, it was found that luciferase gene was expressed at the significant level in immune organs such as gill and spleen, located far from the injected site, suggesting the systemic distribution of the intramuscularly injected DNA in olive flounder.

• Fisheries and Aquatic Sciences
• /
• v.6 no.2
• /
• pp.101-104
• /
• 2003

A cDNA clone for the olive flounder (Paralichthys olivaceus) transferrin receptor (fTfR) was isolated from a leukocytes cDNA library. The fTfR gene consisted of 2,319 bp encoding 773 amino acid residues. The amino acid sequence alignment of the fTfR showed that their size and hydrophobic profile are similar. In addition, the Tyr-Thr-Arg-Phe (YTRF) motif that is the recognition signal for high-efficiency endocytosis, is conserved very well. This motif is important for functional properties of TfR. The deduced amino acid sequence had $42.4-42.9\%$ identities with the previously reported TfRs of vertebrates. The fTfR was expressed in the blood, kidney, spleen, and liver of healthy olive flounder by the Northern blot hybridization.