• Applied Microscopy
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• 제27권4호
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• pp.357-372
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• 1997

To study the age-related morphological differences of the retinal pigment cells and Bruch's membrane of mouse, retinae of one week-old, five weeks-old, eight weeks-old, six months-old, twelve months-old, eighteen months-old, twenty-four months-old and thirty months-old ICR mice were dissected out under anesthesia. Pieces of the tissue taken from the posterior region of the retina were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde (0.1 M Millonig's phosphate buffer, pH 7.3), and 1% osmium tetroxide (0.1 M Millonig's phosphate buffer, pH 7.3), and embedded in araldite mixture. The ultrathin sections were stained with uranyl acetate and lead citrate, and were observed under a JEM 100 CX-II electron microscope. Observed results were as follows: 1. Retinae of one week old mouse exhibit that some parts of the pigment cell provided with basal foldings, whereas other parts of the one contain without basal foldings. After (ive weeks-old, all retinal pigment cells have the basal infoldings. 2. In the one week-old, stage 1 and stage 2 melanosomes were observed in the retinal pigments cells, but after five weeks-old, most of the retinal pigment cells contain some matured stage melanosomes (stage III and stage IV). 3. The phagosomes in the retinal pigment cells were increased during aging. 4. After eighteen months-old, electron dense materials are observed within the basal infoldings. 5. After eighteen months-old, the thickness of the Bruch's membrane is prominently increased. The thickness of the basal laminae of the pigment cell and the choriocapillary endothelium is more prominently increased as compared with that of the other components of the Bruch's membrane. 6. The thickness of the basal lamina of the pigment cell is more prominently increased as compared with that of the choriocapillary endothelium on aging. From the above results, it was suggested that the pigment cell and Bruch's membrane matures structurally In five weeks, and the function of the pigment cell is prominently suppressed around eighteen months-old, and thereafter the functional suppression is continued on aging.

• 대한유전성대사질환학회지
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• 제10권1호
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• pp.59-66
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• 2010

Enzyme replacement of therapy (ERT) is one of the most promising therapeutic strategies for the treatment of lysosomal storage disorders. ERT is available in three types of Mucopolysaccharidosis (MPS): for MPS I (Aludrazyme$^{(R)}$), MPS II (Elaprase$^{(R)}$) and MPS VI (Naglazyme$^{(R)}$) patients who are over 5 years old. But recently, early diagnosis can be done by expert clinicians and even in prenatal case. We describe the case of ERT under 5 years old MPS patients. Up to June, 2010 in Samsung Medical Center, there are 6patients who were diagnosed as MPS and started ERT under 5 years old. 3 patients were MPS I, 3 patients were MPS II. 2 patient who was diagnosed as MPS I was female and others were male. Their age at diagnosis were 4 to 37month-old (4, 13, 16, 25, 27, 37 month-old) and they are now 9 to 60 month-old (9, 39, 32, 81, 60 month-old). The youngest patient was started ERT at 4 month-old and others were started at their 13 to 49 month-old (13, 29, 27, 28, 49 month-old). First manifested symptoms of patients were macrocephaly, kyphosis and coarse face appearance. Especially, in 2 of them, one was MPS I and the other was MPS II had elder brother with same disease. And the youngest one was diagnosed by the iduronate-2-sulfatase (IDS) gene analysis from chorionic villi sampling. His mother knew that she was a heterozygous carrier of IDS gene mutation because her younger brother died from MPS II. All of them confirmed as MPS by the enzyme assay in leukocytes and fibroblast skin culture. We started ERT with ${\alpha}$-L-iduronidase(Aldurazyme$^{(R)}$) to MPS I and did recombinant human iduronate-2-sulfatase (Elaprase$^{(R)}$) to MPS II patients as recommended dose as over 5 years old. But for MPS II patient who was 4 month old, we started ERT by recombinant human IDS (Elaprase$^{(R)}$) with reduced dose 0.1 mg/kg and increased dose every 2 weeks by 0.1mg/kg up to 0.5mg/kg IV infusion. During ERT, all patients had no adverse effects and the excretion of GAGs were decreased. We have evaluated other clinical symptoms such as liver/ spleen volume, heart function and neurologic evaluation. We describe a successful ERT to MPS I and MPS II patient under 5 years old without any adverse event. It indicates that ERT in young children are well tolerated and that it has several effects which may confer clinical benefits with long-term therapy.

• Restorative Dentistry and Endodontics
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• 제26권2호
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• pp.141-152
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• 2001

Eliminating the infecting bacteria of the root canal system and preventing reinfection must be the main objectives of all endodontic works. None of commercially available root canal sealers have the properties of desirable tissue compatibility and strong antibacterial activity. The purpose of this study is to develope an ideal root canal sealer using commercially available polyphosphate (polyP), Calgon, which is known to be antibacterial and safe. For the study. resin type AH26, zinc oxide eugenol type Tubli Seal. Ca(OH)$_2$ type Apexit as base sealers for polyP (0~3%) and para formaldehyde containing N2 as a control base were selected. Specimens (3$\times$4mm) of the sealers were prepared in a 37$^{\circ}C$ incubator for 3 and 10 days and their antibacterial activity against streptococci and black pigmented anaerobic rods was observed using an agar diffusion method. The result were as follows: 1. Among 3 day old root canal sealers. N2 as a positive control showed the strongest antibacterial effect. followed by AH26. Tubli Seal and. Apexit which barely showed antibacterial activity against the test bacteria. In contrast. 10 day old AH26 showed a greater antibacterial activity than 10 day old N2. 2. All sealer specimens showed a greater antibacterial activity against black pigmented anaerobic rods than streptococci. Three day old ones appeared to be more antibacterial than 10 day old ones except for Apexit. 3. As compared to N2, 3 day old AH26 demonstrated a similar antibacterial activity against black pig mented anaerobic rods but to a lesser extent to streptococci. Ten day old AH26 showed a greater antibacterial activity against black pigmented anaerobic rods than 10 day old N2. 4. As compared to AH26. Tubli Seal generally revealed a lower antibacterial activity but it showed a greater antibacterial activity aginst S. gordonii Challis. 5. Enhancement of antibacterial activity by polyP was more clearly observed when it was added to Ca(OH)$^{\circ}C$ based root canal sealers. Tubli Seal and N2. 6. The addition of polyP enhanced the antibacterial activity of 3 day old AH26 against S. gordonii G9B (16%) and Challis (29%), and P. gingivalis 2561 (24%) only. Moreover, polyP failed to increase antibacterial activity of 10 day old AH26 against the test strains but P. gingivalis A7A1 28(13%). 7. The addition of polyP increased the antibacterial effect of 3 day old Tubli Seal on several test bacteria including s. mutans GS 5 (50%). s. gordonii G9B (47%) and Challis (122%). and all the test strains of P. gingivalis (13~35%) except for 9 14K 1. The addition of polyP to 10 day old Tubli Seal increased antibacterial activity of the root canal sealer against most test strains. 8. 3 day old Apexit failed to show antibacterial activity. if any very little against S. mutans GS 5 and Pr. intermedia ATCC 49046. However. polyP increased its antibacterial activity by 50 and 69%, respectively. Increase of antibacterial activity of 10 day old Apexit by polyP was more clearly observed than that of 3 day old one.

• 한국산학기술학회논문지
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• 제16권6호
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• pp.3889-3901
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• 2015

70세 이상 후기노인의 사회적자본이 건강관련 삶의 질에 미치는 영향을 알아보고자 M시의 70세 이상 후기노인 258명에 대해 1차는 2013년 10월 15일부터 11월 15일까지, 2차는 2014년 12월 1일부터 12월 30일까지 설문조사를 실시하였다. 수집된 자료는 SPSS 18.0과 AMOS 21.0 프로그램의 구조방정식 모형을 활용하여 분석하였으며, 매개효과의 통계적 유의성을 분석하기 위해 소벨검증(sobel-test)을 실시하였다. 사회적자본이 건강관련 삶의 질에 미치는 요인은 성별, 교육수준, 동거여부, 운동 여부, 수면시간, 도움요청가족수, 도움요청친척수, 일반신뢰가 영향을 미치는 것으로 나타났다(p<0.05). 연구 모형을 검증한 결과 사회적자본은 건강관련특성에 직접적 영향을 미치고, 건강관련특성은 삶의 질에 직접적 영향을 미치는 것으로 나타났다. 사회적자본은 건강관련 삶의 질에 간접접인 영향을 미치는 것으로 나타났다. 연구 결과를 통해 후기 노인들의 사회적자본이 건강관련 삶의 질에 영향을 미치는 요인들 중의 하나로 인식될 수 있으며 건강관련 삶의 질을 향상시키고, 사회적자본 수준을 증대시키기 위한 정책마련을 제안한다.

• Journal of Forest and Environmental Science
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• 제37권4호
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• pp.315-322
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• 2021

The aim of this study was to investigate the sensitivity to environmental stress, and changes in the photosynthesis capacity in Abies koreana seedlings by age and to suggest the most effective age for restoration. To identify these physiological characteristics of A. koreana, the chlorophyll fluorescence and photosynthetic capacity of 1-, 2-, 3-, 5- and 6-year-old A. koreana seedlings were observed from June 2020 to June 2021. The maximum quantum efficiency of Photosystem II (Fv/Fm), a chlorophyll fluorescence measurement parameter, was strongly positively correlated with the monthly average temperature (1-year-old seedling: r=0.8779, 2-year-old seedling: r=0.8605, 3-year-old seedling: r=0.8697, 5-year-old seedlings: r=0.8085, and 6-year-old seedlings: r=0.8316). The Fv/Fm values were the lowest in winter (November 2020-March 2021). In addition, the Fv/Fm values of 1-, 2-, and 3-year-old seedlings in winter were lower than that of 5- and 6-year-old seedlings, while the Fv/Fm values in summer were relatively higher than those in winter. Further, the Fv/Fm values of seedlings of all ages decreased in August 2020, when the monthly average temperature was the highest. In particular, 1-year-old to 3-year-old seedlings showed Fv/Fm values less than 0.8. Further, the photosynthetic capacity measured in August 2020 increased with increasing seedling age. The analysis of variance results for summer Fv/Fm values showed significant differences in age-specific averages (p<0.05), and Duncan's multiple range test showed significant differences between 5- and 6-year-old seedlings and 1-, 2-, and 3-year-old seedlings (p<0.05). These results suggested that the 5- and 6-year-old seedlings were less sensitive to environmental stress and showed better photosynthetic capacity than the 1-, 2-, and 3-year-old seedlings. Therefore, 5-year-old or older A. koreana seedlings can be used as restoration materials because they can show increased adaptability and stable growth during transplantation due to their relatively high environmental resistance and photosynthetic capacity.

• 한국산학기술학회논문지
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• 제20권6호
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• pp.332-341
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• 2019

본 연구는 전기노인과 후기노인의 수면의 질을 조사하고 수면의 질에 영향을 미치는 요인을 비교하기 위한 서술적 조사연구이다. 연구 대상은 편의표출법을 적용하여 G도에 거주하는 재가 노인을 대상자로 200명을 선정하였다. 구조화된 설문지를 이용하여 사회 인구학적 특성, 생활습관 및 건강관련 특성, 우울, 그리고 수면의 질을 조사하였다. 자료분석은 IBM SPSS WIN/21.0을 이용하여 ${\chi}^2-test$, t-test, ANOVA(Scheffe's test), Pearson's Correlation coefficient, Multiple Regression을 이용하여 분석하였다. 연구결과는 다음과 같았다. 수면의 질은 전기노인에 비해 후기노인이 통계적으로 유의하게 나빴다. 상관관계를 분석한 결과, 전기노인에서는 우울(r=-.22, p=.038)만이 수면의 질과 유의한 부적 상관관계가 나타났다. 후기노인에서는 우울(r=-.19, p=.045)과 주관적 건강상태(r=-.29, p=.002)가 수면의 질과 유의한 부적 상관관계가 있었다. 수면의 질에 영향을 미치는 요인을 분석한 결과, 전기노인에서는 직업, 만성질병 그리고 경제수준으로 나타났고, 후기노인에서는 만성질병, 주관적 건강상태 그리고 흡연이 유의하게 나타났다. 본 연구 결과는 노인의 수면 건강을 향상시킬 수 있는 연령별 맞춤형 전략 수립에 기초자료로 활용될 수 있을 것이다.

• 한국비블리아학회지
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• 제35권1호
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• pp.97-125
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• 2024

본 연구의 목적은 고지도에 대한 정보 접근성을 높이고 나아가 고지도를 이용한 서비스 및 정보제공 등 고지도의 효율적인 활용방안에 대하여 논의하는 것에 있다. 이를 위하여 고지도 정보를 제공하는 국내 기관의 정보검색시스템을 조사하고 해당 웹 사이트에서 고지도 자료 검색 및 정보 접근 방법을 탐색하였다. 이 외에 각 기관의 홈페이지와 도록 및 연구서, 간행물 등을 참고하여 국내 도서관 및 박물관의 고지도 소장현황을 분석하였다. 분석 결과, 전국 국·공립 및 대학 도서관과 박물관 등 76개 기관에 약 2,200여점의 고지도가 소장되어 있었다. 고지도 소장 각 기관에서는 영인본 간행, 전시회 및 도록, 목록 및 총서 등의 연구서 편집서의 간행 등 출판사업을 수행하고 있었다. 그러나 고지도의 희귀성, 유일본 등의 자료적 특성으로 원문 자료의 열람 및 이용이 제한적이다. 고지도를 효율적으로 활용하기 위해서는 첫째, 고지도 정보서비스 접근성 향상 및 학술정보서비스 확대가 필요하다. 둘째, 지역의 정체성을 반영한 아카이브 구축을 위한 자료로 고지도 활용을 제안한다. 셋째, 고지도 자료에 대한 지식과 인문학적 소양을 바탕으로 정보를 선별하여 제공하는 전문인력 양성이 필요하다.

• 한국문헌정보학회지
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• 제52권3호
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• pp.193-214
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• 2018

이 연구는 중국의 고문헌 관리 법제화에 대한 조사 분석을 목적으로 진행하였다. 우선 중국의 법체계와 고문헌 관리 정책을 살피고, 4종의 법률과 6종의 행정법규, 4종의 부문규장 및 13종의 행정규범성문건을 조사하였다. 그중 고문헌 관리 내용을 다루고 있는 1) 2013년의 법률 '중국문물보호법', 2) 1981년의 행정법규 '중국고문헌정리지시', 3) 1986년의 부문규장 '중의고문헌 연구정리출판 관리세칙', 4) 2001년의 부문규장 '문물소장품 등급결정 표준', 5) 2003년의 부문규장 '문물경매관리 임시규정'을 분석하였다. 이를 바탕으로 1) 고문헌 관리 정책의 중요성, 2) 국가 차원의 고문헌 관리, 3) 교육 확대와 인적자원의 양성, 4) 고문헌 관리 전문기구의 설치, 5) 중의고문헌 부문의 특별 관리 등의 중국 고문헌 관리의 시사점을 도출하였다.

• 한국정보전자통신기술학회논문지
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• 제10권4호
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• pp.279-287
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• 2017

The aim of this study is to verify the difference in the level of happiness and preparation for old age according to the general characteristics of senior welfare center attendees. The subjects of the study were 148 attendees of OO senior welfare center in Seoul and a survey was conducted from July 4 to October 7, 2016. The method of analysis was by using the SPSSWIN 18.0 program, a 5% level of significance was verified in a frequency analysis, t-test, one-way ANOVA, correlation analysis, and multiple regression analysis. The results showed that first, from investigating the difference in the level of preparation for old age according to general characteristics, there was a significant difference according to age, occupation, monthly income, and physical condition but there was no significant difference according to gender and level of education. Second, as a result of observing the difference in the level of happiness according to general characteristics, although there was a significant difference according to occupation and monthly income, the difference level according to gender, age, level of education, health condition was less than a 5% level of significance. Third, from observing the correlation between the level of happiness and preparation of old age, it was found that happiness was positively correlated to preparation for old age in the following order: physical preparation for old age ((r=.576, p<.001), economic preparation for old age (r=.570, p<.001), psychological preparation for old age (r=.526, p<.001), social preparation for old age (r=.525, p<.001). It is forecasted that this study will be used as base line data for improvement of happiness and preparation for old age for the elderly according to general characteristics.

• 한국도로학회논문집
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• 제18권5호
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• pp.21-29
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• 2016

PURPOSES : The purpose of this study is to suggest a specific investigation guideline to decide priority of repairing old concrete pavements that pile up substantially. METHODS : In this study, a principle of division of homogeneous sections was proposed to reflect the characteristics of the pavement reasonably in the specific investigation results. In addition, a checklist and guideline of field investigation were suggested for the old concrete pavement sections, which require inspection toward their durability and structural performance. Furthermore, the items of laboratory test necessary to the old concrete pavement were suggested based on the existing laboratory test considering characteristics of the old concrete pavement. The present condition of the old concrete pavement could be analyzed by the test results. RESULTS : A method of division of homogeneous sections suitable for the specific investigation of the old concrete pavement was suggested. The proportions of distress severity of pavement sections were compared by distress type to figure out the present state of the old concrete pavement. Scaling, durability cracking (or alkali-silica reaction), and longitudinal spalling were selected as the most severe distress types. The detailed positions of the sections were also suggested. The checklist of the specific investigation was categorized by field survey and laboratory test, and its evaluation criteria were proposed. The three types of the sections of durability cracking (or alkali-silica reaction), bridge connection, and asphalt overlay were selected as the sections of the field survey. The compressive strength, void structure, and chloride penetration depth were suggested as the items of the laboratory test. CONCLUSIONS : A fundamental level of the guideline was suggested in this study to resolve the problem of old concrete pavement. Appropriate guidelines related to the repair of the old concrete pavement should be provided by performing additional research efforts.