• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2001.09a
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• pp.111-114
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• 2001

Thermal desorption process is valuable for the remediation of oil contaminated site. The system is physical separation process by volatizing oil contaminants from soil matrixes and is not designed to provide high levels of oil destruction. The process is not incineration, because the decomposition of oil materials is not the desired result, although some decomposition may occur. The physical and chemical properties that influence the design and operation of the system include boiling points, soil sorption characteristics, aqueous phase solubility, thermal stability, contaminating oil concentration, moisture contents, particle size distribution and etc.

• Journal of Microbiology and Biotechnology
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• v.18 no.12
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• pp.1966-1974
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• 2008

A typical tropical soil from the northeast of Brazil, where an important terrestrial oil field is located, was accidentally contaminated with a mixture of oil and saline production water. To study the bioremediation potential in this area, molecular methods based on PCR-DGGE were used to determine the diversity of the bacterial communities in bulk and in contaminated soils. Bacterial fingerprints revealed that the bacterial communities were affected by the presence of the mixture of oil and production water, and different profiles were observed when the contaminated soils were compared with the control. Halotolerant strains capable of degrading crude oil were also isolated from enrichment cultures obtained from the contaminated soil samples. Twenty-two strains showing these features were characterized genetically by amplified ribosomal DNA restriction analysis (ARDRA) and phenotypically by their colonial morphology and tolerance to high NaCl concentrations. Fifteen ARDRA groups were formed. Selected strains were analyzed by 16S rDNA sequencing, and Actinobacteria was identified as the main group found. Strains were also tested for their growth capability in the presence of different oil derivatives (hexane, dodecane, hexadecane, diesel, gasoline, toluene, naphthalene, o-xylene, and p-xylene) and different degradation profiles were observed. PCR products were obtained from 12 of the 15 ARDRA representatives when they were screened for the presence of the alkane hydroxylase gene (alkB). Members of the genera Rhodococcus and Gordonia were identified as predominant in the soil studied. These genera are usually implicated in oil degradation processes and, as such, the potential for bioremediation in this area can be considered as feasible.

• Microbiology and Biotechnology Letters
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• v.35 no.2
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• pp.150-157
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• 2007

The advanced bioremediation of diesel-contaminated soil through the exploration of bacterial interaction with plants was studied. A diesel-degrading rhizobacterium, Rhodococcus sp.412, and a plant species, Zea mays, having tolerant against diesel was selected. Zea mays was seeded in uncontaminated soil or diesel-contaminated soil with or without Rhodococcus sp. 412. After cultivating for 30 days, the growth of Zea mays in the contaminated soil inoculated with Rhodococcus sp. 412 was better than that in the contaminated soil without the bacterium. The residual diesel concentrations were lowered by seeding Zea mays or inoculating Rhodococctis sp. 412. These results Indicate that the simultaneous use of Zea mays and Rhodococcus sp. 412 can give beneficial effect to the remediation of oil-contaminated soil. Bacterial community was characterized using a 16S rDNA PCR and denaturing gradient gel electrophoresis (DGGE) fingerprinting method. The similarities of DGGE fingerprints were $20.8{\sim}39.9%$ between the uncontaminated soil and diesel contaminated soil. The similarities of DGGE fingerprints were $21.9%{\sim}53.6%$ between the uncontaminated soil samples, and $31.6%{\sim}50.0%$ between the diesel-contaminated soil samples. This results indicated that the structure of bacterial community was significantly influence by diesel contamination.

• Journal of Soil and Groundwater Environment
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• v.16 no.5
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• pp.24-30
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• 2011

Tidal flats which are ecologically sensitive, are hard to remediate once they are contaminated by oil spill accidents. Traditional oil remediation measures focus on removal efficiency, and their improper implementation can adversely affect crude oil contaminated coastal areas and greatly disrupt the structure and functions of crude oil contaminated tidal flats. In this study, the oil degradation due to the implementation of remediation measures naturally enhanced using air and natural oil sorbents was evaluated in the lower strata of tidal flats. The effects of air and natural oil sorbents on oil degradation for two concentration levels (< 500 ppm and > 500 ppm) were tested at artificially contaminated tidal flats. Fifty days after these treatments, the natural oil sorbent treatment showed the lowest total petroleum hydrocarbon (TPH) concentration ($4.46{\pm}1.47%$) at the low concentration level, whereas both air and natural oil sorbent treatments showed high degradation efficiencies at the high concentration level ($29.30{\pm}4.39%$). Although the phosphatase activity decreased for all treatments, there was no significant difference between the decreases for the different treatments; on the other hand, B-glucosidase activities were high for both air and natural oil sorbent treatments. Although degradation efficiencies decreased as the concentration increased, the air provision and natural oil sorbent treatment could be an effective ecological restoration measure for oil contaminated tidal flats while minimizing the environmental impact of the remediation efforts.

• Journal of Soil and Groundwater Environment
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• v.17 no.1
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• pp.8-12
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• 2012

This study is maintains the condition of high temperature (above $600^{\circ}C$) within a short time using on microwave and high temperature heating elements. And removal characteristics according to changes in soil moisture, microwave power and temperature through the decomposition of the contaminated soil by oil. The difficulty resolvability material was sort of lubricating oil having long carbocyclic (C18-C50) and TPH removal rate reached 85.2% at 6 kW and $700^{\circ}C$ and thus the contaminant was removed 1,788 mg/kg within a process time of 40 minutes. In the case of light oil, gasoline contaminated soil, the removal amount showed 567 mg/kg and the treatment rate reached 98.4% at 6 kW, $500^{\circ}C$ and 20 minute. In the case of non-resolvability reached TPH concentrations on 2,000 mg/kg of worrisome level of soil contamination in the 3 zones at 6 kW, $700^{\circ}C$ and 30 minute. At the time, showed up processing costs 8,173 won per ton.

• Korean Journal of Environmental Agriculture
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• v.19 no.3
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• pp.191-193
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• 2000

To find out the countermeasure to plant damage at soil contaminated with oil, several adsorbents such as muck, peat, sawdust and PEAT SORB were treated at diesel oil contaminated soil. As the results, sawdust and PEAT SORB showed better effect of oil adsorption than muck and peat. Removal rate of diesel oil with sawdust treatment was higher than 95% at the condition which the ratio of adsorbent amount to oil was higher than 1:2(w/v). And the releasing amount of oil from adsorbent-oil complex was very small. With the oil treatment of $4,000\;L{\cdot}ha^{-1}$ at tillering stage, rice plant height and chlorophyll content were lower than control at non-adsorbent treatment, but those were increased at sawdust treatment.

• Journal of Microbiology and Biotechnology
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• v.11 no.4
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• pp.716-719
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• 2001

The mobilization and biodegradation of phenanthrene in soil was enhanced by using paraffin oil, which was stabilized by the addition of a surfactant (Brji 30). The ratio of paraffin oil/Brij 30 was determined by measuring the change in the critical micelle concentration. When only surfactant was used, the stabilized paraffin oil emulsion could dissolve more phenanthrene in the water phase. Column experiment showed increased phenanthrene mobilization from the contaminated soil. The phenanthrene mobilized in the paraffine oil/Brij 30 emulsion was biodegraded faster than that in water phase or surfactant solution. This result indicates that a paraffin oil/surfactant system can be effectively used for the removal of PAH from contaminated soil.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.04a
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• pp.297-300
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• 2004

The various microbial tests were performed to determine bioremediation agent capacity for eight strains isolated from the oil contaminated regions. Two tests for isolated strains were conducted such as cell hydrophobicity and emulsifying activity. The biodegradation of SHM (saturated hydrocarbon mixture) and AHM (aromatic hydrocarbon mixture) with the strains also was carried out. The strains having higher cell hydrophobicity and emulsifying activity degraded petroleum oil effectively. The degradation capacity for SHM was represented more than 90% in YS-7 and WLH-1 of isolated strains, and KH3-2 were capable of degrading AHM. Especially, WLH-1 as yeast was shown more than two or three times in the degradation capacity of automobile engine lubricants and the biomonitoring results of contaminated soil for residual oil degrading test showed that the hydrocarbon biodegradation was increased in the second treatment by this strain.

• Geomechanics and Engineering
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• v.20 no.3
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• pp.207-220
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• 2020

Clay soils have a big potential to become contaminated with the oil derivatives because they cover a vast area of the earth. The oil derivatives diffusion in the soil lead to soil contamination and changes the physical and mechanical properties of the soil specially clay soils. Soil stabilization by using new material is very important for geotechnical engineers in order to improve the engineering properties of the soil. The main subjects of this research are a- to investigate the effect of the cement and epoxy resin mixtures on the stabilization and on the mechanical parameters as well as the microstructural properties of clay soils contaminated with gasoline and kerosene, b- study on the phenomenon of clay concrete development. Practical engineering indexes such as Unconfined Compressive Strength (UCS), elastic modulus, toughness, elastic and plastic strains are all obtained during the course of experiments and are used to determine the optimum amount of additives (cement and epoxy resin) to reach a practical stabilization method. Microstructural tests were also conducted on the specimens to study the changes in the nature and texture of the soil. Results obtained indicated that by adding epoxy resin to the contaminated soil specimens, the strength and deformational properties are increased from 100 to 1500 times as that of original soils. Further, the UCS of some stabilized specimens reached 40 MPa which exceeded the strength of normal concrete. It is interesting to note that, in contrast to the normal concrete, the strength and deformational properties of such stabilized specimens (including UCS, toughness and strain at failure) are simultaneously increased which further indicate on suitability and applicability of the current stabilization method. It was also observed that increasing cement additive to the soil has negligible effect on the contaminated soils stabilized by epoxy resin. In addition, the epoxy resin showed a very good and satisfactory workability for the weakest and the most sensitive soils contaminated with oil derivatives.

• Environmental Engineering Research
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• v.24 no.3
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• pp.484-494
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• 2019

This paper presents a preliminary investigation of the optimum nutrients combination required for bioremediation of spent-engine oil contaminated soil using Box-Behnken-Design. Three levels of cow-manure, poultry-manure and inorganic nitrogen-phosphorus-potassium (NPK) fertilizer were used as independent biostimulants variables; while reduction in total petroleum hydrocarbon (TPH) and total soil porosity (TSP) response as dependent variables were monitored under 6-week incubation. Ex-situ data generated in assessing the degree of biodegradation in the soil were used to develop second-order quadratic regression models for both TPH and TSP. The two models were found to be highly significant and good predictors of the response fate of TPH-removal and TSP-improvement, as indicated by their coefficients of determination: $R^2=0.9982$ and $R^2=1.000$ at $p{\leq}0.05$, respectively. Validation of the models showed that there was no significant difference between the predicted and observed values of TPH-removal and TSP-improvement. Using numerical technique, the optimum values of the biostimulants required to achieve a predicted maximum TPH-removal and TSP-improvement of 67.20 and 53.42%-dry-weight per kg of the contaminated soil were as follows: cow-manure - 125.0 g, poultry-manure - 100.0 g and NPK-fertilizer - 10.5 g. The observed values at this optimum point were 66.92 and 52.65%-dry-weight as TPH-removal and TSP-improvement, respectively.