• Journal of Plant Biotechnology
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• v.47 no.3
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• pp.185-193
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• 2020

The number of commercially approved gene-edited crops is gradually increasing, and in South Korea, it has led to intense investment in gene-edited crop development to increase international competitiveness. However, as with genetically modified crops, the safety of gene-edited crops regarding unexpected risks for humans and the environment is subject to an ongoing debate. In particular, unintentional "off-target effects" have become the center of controversy. In this review, we discuss typical plant characteristics (including somatic variation and ploidy), the extent of various off-target effects in genetically modified crops generated via horizontal transfer in nature, and the off-target effects in commercial genetically modified crops. We conclude that most off-target effects possibly occurring in gene-edited crops are not expected to be critically harmful to humans or the environment. Therefore, existing regulation for genetically modified crops should be enough for the risk assessment of gene-edited crops.

• Molecules and Cells
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• v.38 no.6
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• pp.475-481
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• 2015

Programmable nucleases, which include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and RNA-guided engineered nucleases (RGENs) repurposed from the type II clustered, regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system are now widely used for genome editing in higher eukaryotic cells and whole organisms, revolutionising almost every discipline in biological research, medicine, and biotechnology. All of these nucleases, however, induce off-target mutations at sites homologous in sequence with on-target sites, limiting their utility in many applications including gene or cell therapy. In this review, we compare methods for detecting nuclease off-target mutations. We also review methods for profiling genome-wide off-target effects and discuss how to reduce or avoid off-target mutations.

• The Journal of Information Technology
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• v.3 no.3
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• pp.67-76
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• 2000

Internet user are very important target market in off-line. Also as being activated e-business, they become core target market in on-line. It comes the present question to the business that marketer has to develop the most suitable marketing method by analysis of consumer's purchasing behavior In this study, we analysis internet user's purchasing behavior and consider the way which is the most suitable internet advertisement using an analysis of census.

• Proceedings of the Korean Information Science Society Conference
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• 2006.06a
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• pp.43-45
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• 2006

Unlike RNA interference, whose usage is limited to eukaryotic cells, Peptide Nucleic Acid (PNA) technique is applicable to both eukaryotic and prokaryotic cells. PNA has been proven to be an effective agent for blocking gene expressions and has several advantages over other antisense techniques. Here we developed a parallel computing software that provides the ideal sequences to design PNA oligos to prevent any off-target effects. We applied a new approach in our location-finding algorithm that finds a target gene from the whole genome sequence. Message Passing Interface (MPI) was used to perform parallel computing in order to reduce the calculation time. The software will help biologists design more accurate and effective antisense PNA by minimizing the chance of off-target effects.

• Journal of IKEEE
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• v.23 no.2
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• pp.375-379
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• 2019

$ZnO-SnO_2(ZTO)$ was deposited by RF magnetron sputtering using a ceramic target whose Zn atomic ratio to Sn is 2:1 as a target, and the crystal structure variation with thermal treats was investigated. Transparent thin film transistors (TTFT) were fabricated using the ZTO films as active layers. About 100 nm-thick $Si_3N_4$ film grown on 100 nm-thick $SiO_2$ film was adopted as gate dielectrics. The mobility, threshold voltage, $I_{on}/I_{off}$, and interface trap density were obtained from the transfer characteristics of ZTO TTFTs. The effects of substrate temperature, and post-annealing on the property variation of ZTO TTFT were analyzed.

• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.205-216
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• 2019

Since the RNA interference (RNAi) had been discovered in many organisms, small non-coding RNA-mediated gene silencing technology, including RNAi have been widely applied to analysis of gene function, as well as crop improvement. Despite the usefulness of RNAi technology, RNAi transgenic crops have various potential environmental risks, including off-target and non-target effects. In this study, we developed methods that can be effectively applied to environmental risk assessment of RNAi transgenic crops and verified these methods in 35S::dsRNAi_eGFP rice transgenic plant we generated. Off-target genes, which can be non-specifically suppressed by the expression of dsRNAi_eGFP, were predicted by using the published web tool, pssRNAit, and verified by comparing their expressions between wild-type (WT) and 35S::dsRNAi_eGFP transgenic rice. Also, we verified the non-target effects of the 35S:: dsRNAi_eGFP plant by evaluating horizontal and vertical transfer of small interfering RNAs (siRNAs) produced in the 35S::dsRNAi_eGFP plant into neighboring WT rice and rhizosphere microorganisms, respectively. Our results suggested that the methods we developed, could be widely applied to various RNAi transgenic crops for their environmental risk assessment.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.10
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• pp.1341-1347
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• 2002

In order to improve efficiency of a system with three-dimensional flow characteristics, this paper presents a new method that overcomes three-dimensional effects by using two-dimensional CFD and neural network. The method was applied to shape optimization of cut-off in a multi-blade fan/scroll system. As the entrance conditions of two-dimensional CFD, the experimental values at the positions out of the inactive zone were used. The distributions of velocity and pressure obtained by two-dimensional CFD were compared with those of three-dimensional CFD and experimental results. It was found that the distributions of velocity and pressure have qualitative similarity. The results of two-dimensional CFD were used for teaming as target values of neural network. The optimal angle and radius of cut-off were determined as 71$^{\circ}$and 0.092 times the outer diameter of impeller, respectively. It is quantified in the previous report that the optimal angle and radius of cut-off are approximately 72$^{\circ}$and 0.08 times the outer diameter of impeller, respectively.

• Physical Therapy Korea
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• v.13 no.4
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• pp.23-29
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• 2006

The purpose of the present study was to examine gaze effects on spatial and kinematic characteristics during a pointing task. Subjects were asked to watch and point to an aimed target (2 mm in diameter) displayed on a vertically mounted board. Four gaze conditions were developed as combinations of "seeing-aiming" in terms of the eye movements: Focal-Focal (F-F), Focal-Fixing (F-X), Fixing-Focal (X-F), and Fixing-Fixing (X-X). Both the home target and an aimed target were presented for 1 second and then were disappeared in F-F and X-F. In X-F and X-X, only an aimed target disappeared after 1 second. Subjects were asked to point (with index finger tip) to an aimed target accurately as soon as the aimed target was removed. A significant main effect of gaze was found (p<.01) for normalized movement time. Peripheral retina targets had significantly larger absolute error compared to central retina targets on the x (medio-lateral) and z (superior-inferior) axes (p<.01). A significant undershooting to peripheral retina targets on the x axis was found (p<.01). F-F and X-F had larger peak velocities compared to F-X and X-X (p<.01). F-F and X-F were characterized by more time spent in the deceleration phase compared to F-X and X-X (p<.01). The present study demonstrates that central vision utilizes a form of on-line visual processing to reach to an object, and thus increases spatial accuracy. However, peripheral vision utilizes a relatively off-line visual processing with a dependency on proprioceptive information.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.35 no.3
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• pp.301-311
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• 1999

The investigation to evaluate the possible effects of fish behaviour on acoustic target strength was carried out during the 1997 and 1998 hydroacoustic-demersal trawl surveys in the southern waters of Korea.The swimming speed and the target strength of individual, acoustically resolved fished swimming through the sound beam were measured using the split-beam tracking method on board R/V Kaya.The results obtained can be summarized as follows:1. The alongship and athwartship angles between -3dB poionts of a hull mounted 38 kHz split beam tranducer used in these surveys was >$3.76^{\circ}\;and\;6.74^{\circ}$ respectively, and the equal energy contour obtained from the measured beam pattern showed approximately the circular pattern. 2. The swimming speed measured off the south coast of Sorido in 23 January 1997 ranged 0.10 to 0.80 m/s with the average swimming spped of 0.36 m/s, and the target strength ranged -64.8 to -31.7 dB with the average target strength of -52.7 dB. The most dominant species sampled in this survey area were Japanese scaled sardine, Sardinella zunasi and Konoshiro gizzard shad, Konosirus punctatus, respectively.3. The swimming speed measured off the east coast of Kojedo in 24 March 1997 ranged 0.10 to 1.10 m/s with the average swimming speed of 0.40 m/s, and the target strength ranged -64.8 to -51.5 dB with the average target strength of -59.2 dB. The most dominant species sampled in this survey area were Swordtip squid, Photololigo edulis, Konoshiro gizzard shad and Japanese flying squid, Toddarodes pacificus, respectively and the swimming activity of these species seems to be controlled at speeds between 0.20 and 0.60 m/s. 4. The swimming speed measured the south coast of Kojedo in 25 March 1997 ranged 0.10 to 1.40 m/s with the average swimming speed of 0.51 m/s and the target strength ranged -64.3 to -47.7 dB with the average target strength of -55.1 dB. The most dominant species sampled in this survey area were Swordtip squid, Blotchy sillage, Sillago maculata and japanese scaled sardine, respectively and the swimming activity of these species seems to be controlled at speeds between 0.20 and 0.70 m/s.5. The swimming speed measured during morning twilight in the southeastern water of Cheju Island in 11 July 1998 ranged 0.20 to 1.0 m/s with the average swimming speed of 0.53 m/s, and the target strength ranged -65.0 to -47.0 dB with the average target strength of -57.1 dB. The most dominant species sampled in this survey area were Swordtip squid, Black scraper, Thamnaconus modesutus and japanese flying squid, respectively and the tile angle ranged$ +28^{\circ}\;to\;+2^{\circ}$ with the average tilt angle of -8.1$^{\circ}$ showing the downward migration.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.30 no.1
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• pp.23-26
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• 2017

Mo doped carbon (C:Mo) thin films were fabricated with various Mo target power densities by unbalanced magnetron sputtering (UBM). The effects of target power density on the surface, structural, and electrical properties of C:Mo films were investigated. UBM sputtered C:Mo thin films exhibited smooth and uniform surfaces. However, the rms surface roughness of C:Mo films were increased with the increase of target power density. Also, the resistivity value of C:Mo film as electrical properties was decreased with the increase of target power density. From the performance of organic thin filml transistor using conductive C:Mo gate electrode, the carrier mobility, threshold voltage, and on/off ratio of drain current (Ion/Ioff) showed $0.16cm^2/V{\cdot}s$, -6.0 V, and $7.7{\times}10^4$, respectively.