• Reproductive and Developmental Biology
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• 제28권1호
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• pp.45-52
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• 2004

To search a new porcine pheromonal odorants, this research for biostimulation and role of pheromone was augmented by means of "control system technologies" to offer a potentially useful and practical way to improve reproductive efficiency in livestock species. Therefore the 13 physicochemical parameters such as similarity indice (S), hydrophobicity (logP) and van der Waals molecule volume (MV) etc. of 54 steroid analogues, which are analogous of substrate molecules, 5$\alpha$-androst-16-en-3-one (P1) and 5$\alpha$-androst-16-en-3-ol (P2) of lipocalin as receptor of pig pheromones were calculated and discussed. The physicochemical properties of these steroid analogues were mainly followed by steric dissimilar of A and D ring in steroid nucleus. And we found that from correlation with S values and MV constants of molecules, the more MV constants are small, the more S values tend to approach 1. Based on this results, the S-values of 4-androsten-3,17-dione (P1-1) and 5 $\alpha$ -androstan-3-one (P2-1) were 1.0, respectively. The two compounds of them were chosen because they showed the same value each other at a side of hydrophobicity, molar refractivity and molecular volume. It is expected that the new two compounds will be able to substitute for P1 and P2, porcine pheromonal odorants.

• Reproductive and Developmental Biology
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• 제29권1호
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• pp.43-48
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• 2005

돼지 웅성 페르몬인 5a-androst-16-en-3-one을 대체할 수 있는 활성 분자를 탐색하여 가축의 생산과 수요를 조절하기 위한 생물학적 자극 통제 수단으로 활용하고자 냄새 분자로서 2-cyclohexyloxytetrahydrofurane (A) 및 2-phenoxytetrahydrofurane (B) 유도체들의 구조 변화와 수용체인 porcine odorant binding protein (pOBP)에 대한 결합친화력 상수(p[Od.]/sub 50/) 사이의 정량적인 구조-활성관계에 관한 분자 HQSAR 모델XI을 유도하였다. 냄새 분자 중에서 cyclohexyl-치환체(A)가 phenyl-치환체(B)보다 높은 결합 친화력을 나타내었으며(A>B) 모델XI은 분자 조각크기(5∼8), 홀로그램 길이(97 bin)의 키랄성(chirality)조건에서 예측성(q²=0.916)과 상관성(r²=0.988)이 매우 양호하였다. 기여도(contribution map)로부터 냄새 분자의 결합 친화력 상수에 기여하는 부분은 2-oxyfuryl group의 C3 및 C5 원자인 반면에 cyclohexyl 고리상 tert-butyl group의 중심 탄소 원자와 furyl group의 C4 원자 부분은 기여하지 않았다.

• Reproductive and Developmental Biology
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• 제30권1호
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• pp.13-19
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• 2006

생물학적 자극통제 수단으로 활용하기 위한 돼지 웅성 페르몬성 분자를 탐색하고자 일련의 냄새 분자로서 2-(cyclo-hexyloxy) tetrahydrofurane 유도체들의 정량적인 구조와 수용체인 porcine odorant binding protein (pOBP)간의 결합 친화력 상수$(p(Od)_{50})$에 대한 비교 분자장 분석(CoMFA)을 실행하였다. 가장 양호한 CoMFA 모델 AIV $(r^2_{cv}.(q^2)=0.886$ 및 $r^2_{ncv}.=0.984$)은 기질 분자 내 입체 중심(chiral center)의 절대 배열이 $C_1(R),C_2(S)$인 분자를 atom based fit 방법으로 배열하였을 경우의 standard field와 indicator field가 조합된 CoMFA장의 조건에서 유도되었다. 이 CoMFA 모델은 입체장 40.8% 정전기장 14.6%및 소수성장 44.6%가 결합 친화력 상수에 영향을 미치는 요소임을 나타내었다. 등고도의 분석 결과로부터 효과적인 결합 친화력 냄새 분자를 수식하는 데 몇 가지 가치 있는 정보를 얻을 수 있었다.

• Reproductive and Developmental Biology
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• 제30권3호
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• pp.169-174
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• 2006

돼지 페르몬성 분자를 탐색하기 위하여 일련의 green odorant로서 기질 분자인 2-(Cyclohexyloxy)tetrahydrofurane 유도체들의 정량적인 구조와 수용체인 porcine odorant binding protein(pOBP) 사이의 결합 친화력 상수($p(Od)_{50}$)에 대한 비교 분자 유사성 지수 분석(CoMSIA)을 실행하였다. 가장 양호한 CoMSIA 모델(I-AI)은 기질 분자내 입체 중심의 절대 배열이 $I:\;C_{1'}(R),\;C_2(S)$인 분자를 atom based fit 정렬하였을 경우의 입체장 조건에서 유도되었으며 PLS 분석 결과, 예측성이 ${r^2}_{cv.}(q^2)=0.856)$ 그리고 적합성이 ${r^2}_{ncv.}=0.964)$ 이었다. 모델의 CoMSIA 등고도 상, pOBP와 냄새 분자 사이의 상호작용으로부터 가장 높은 결합 친화력을 나타내는 분자의 구조적 특정들을 이해할 수 있었다.

• 한국대기환경학회:학술대회논문집
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• 한국대기환경학회 2004년도 춘계학술대회 논문집
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• pp.65-69
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• 2004

The need for developing sustainable solutions for managing the animal waste is vital for the future of the animal industry in North Carolina. As part of that process, the North Carolina Attorney General has concluded that the public interest will be served by the development and implementation of environmentally superior swine waste management technologies appropriate to each category of hog farms. To facilitate in the development, testing, and evaluation of potential technologies it is necessary that all aspects of environmental issues (air, water, soil, odor and odorants, and disease-transmitting vectors and airborne pathogens) be addressed as Part of a comprehensive strategy, Program OPEN (Odor, Pathogens, and Emissions of Nitrogen) Is comprehensively addressing these issues.

• 한국대기환경학회지
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• 제30권3호
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• pp.211-222
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• 2014

Pork belly meat is one of the most preferred food items for many Korean people. The odorants released from cooking of pork belly meat were measured by three kinds of cooking methods (Charcoal-grill (C), Electric Pan (E), and Gas burner-pan (G)). A total of 16 target compounds including carbonyl compounds, volatile organic compounds, and poly aromatic hydrocarbon were selected and analyzed for comparative purposes. Their emission concentrations were quantified using HPLC-UV, GC-MS, GC-TOF-MS, etc. The gas samples collected by Charcoal-grill cooking showed generally enhanced concentrations of most target compounds among all three kinds of cooking methods. In Charcoal-grill, concentration of benzene, formaldehyde and pyrene went up to 543, 516, and 402 ppb, respectively. It the results are compared in terms of the sum of odor intensity, the highest value (4.25) was also seen from Charcoal-grill. The results of this study confirm that the significantly reduced emission of harmful pollutants can be attained, it pork belly meat is cooked by the Gas or electric pan instead of Charcoal-grill.

• 한국식품영양과학회지
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• 제28권2호
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• pp.312-318
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• 1999

Volatile compounds in salt fermented anchovy on the market were analyzed by vacuum simulta neous distillation solvent extraction/gas chromatography/mass spectrometry/olfactometry(V SDE/ GC/MS/O) and aroma extract dilution anlaysis(AEDA). Predominant odorants(Log3FD$\geq$8) in sample were ethyl methylbutanoate(candy like/sweet) and 2 ethyl 3,5 dimethylpyrazine(nutty/baked potato like). Besides these compounds, 6 odorants such as ethyl 3 methylbutanoate(sweet/floral/ candy like), 3 methylbutanal(dark chocolate like), (Z) 4 heptenal(rancid/fish like), (methylthio) propanal(soy sauce /baked potato like), (E,Z) 2,6 nonadienal(melon /cucumber like) and (E,E) 2,4 decadienal(fatty/cooked soybean like) were potent in odor value of salt fermented anchovy. Seven amino acids having high taste value in sample were glutamic acid, aspartic acid(sour and umami taste), lysine, alanine(sweet), histidine, valine, and methionine(bitter).

• Fisheries and Aquatic Sciences
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• 제26권6호
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• pp.393-405
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• 2023

The aim of this research was to enhance the flavor of visceral extracts from skipjack tuna. Flavor precursors and the optimum condition for the Maillard reaction were determined. The flavor extract was prepared from the tuna viscera using Endo/Exo Protease controlled in 3 factors; temperature, enzyme amounts and incubation time. The optimal condition for producing tuna viscera protein hydrolysate (TVPH) was 60℃, 0.5% enzyme (w/w) and 4-hour incubation time. TVPH were further processed to tuna viscera flavor enhancer (TVFE) with Maillard reaction. The Maillard reactions of TVFE were conducted with or without supplements such as xylose, yeast extract and methionine. The Maillard volatile components were analyzed with gas chromatography-mass spectrometry. Sixteen volatiles such as 2-methylpropanal, methylpyrazine, 2,5-dimethylpyrazine, dimethyl disulfide and 2-acetylthaizone were newly formed via Maillard reaction and the similarity of volatile contents from TVPH and TVFE were virtualized using Pearson's correlation integrated with heat-map and principal component analysis. To virtualize aromagram of TVPH and TVFE, odor activity value and odor impact spectrum (OIS) techniques were applied. According to OIS results, 3-methylbutanal, 2-methylbutanal, 1-octen-3-ol 2,5-dimethylpyrazine, methional and dimethyl trisulfide were the potent odorants contributed to the meaty, creamy, and toasted aroma in TVFE.

• 한국가스학회:학술대회논문집
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• 한국가스학회 2006년도 추계학술발표회 논문집
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• pp.163-168
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• 2006

• 한국가스학회:학술대회논문집
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• 한국가스학회 2000년도 추계학술발표회 논문집
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• pp.211-214
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• 2000