• Journal of Microbiology
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• 제37권3호
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• pp.128-135
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• 1999

Among oil-degrading microorganisms isolated from oil-polluted industrial areas, one yeast strain showed high degradation activity of aliphatic hydrocarbons. From the analyses of 18S rRNA sequences, fatty acid, coenzyme Q system, G+C content of DNA, and biochemical characteristics, the strain was identified as Yarrowia lipolytica 180. Y. lipolytica 180 degraded 94% of aliphatic hydrocarbons in minimal salts medium containing 0.2% (v/v) of Arabian light crude oil within 3 days at 25$^{\circ}C$. Optimal growth conditions for temperature, pH, NaCl concentration, and crude oil concentration were 30$^{\circ}C$, pH 5-7, 1%, and 2% (v/v), respectively. Y. lipolytica 180 reduced surface tension when cultured on hydrocarbon substrates (1%, v/v), and the measured values of the surface tension were in the range of 51 to 57 dynes/cm. Both the cell free culture broth and cell debris of Y. lipolytica 180 were capable of emulsifying 2% (v/v) crude oil by itself. They were also capable of degrading crude oil (2%). The strain showed a cell surface hydrophobicity higher than 90%, which did not require hydrocarbon substrates for its induction. These results suggest that Y. lipolytica has high oil-degrading activity through its high emulsifying activity and cell hydrophobicity, and further indicate that the cell surface is responsible for the metabolism of aliphatic hydrocarbons.

• 한국항해항만학회지
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• 제29권3호
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• pp.275-282
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• 2005

한국의 등대는 1903년 6월 팔미도에서 점등된 것을 기점으로 지난 2003년에 건립 100주년을 맞이하였다. 등대는 본래 천혜의 자연조건 속에 입지해 있고, 바다와 관련된 시적 감흥을 간직하고 있으며, 태양에너지 등을 동력원으로 하는 친환경적인 건측시설이다. 이처럼 등대는 그 잠재석 가치와 개발 가능성이 높은 시설이다. 그 가운데 적절하게 개발된 유인등대는 이제 전통적인 기능과 역할에 충실한 항로표지일 뿐만 아니라 온 국민이 향유하고 체험하는 새로운 사설이자 공간이 되였다. 따라서 유인등대를 친수공간으로 개발할 경우 보다 체계적이고 미래지향적인 건축사설, 운영프로그램 그리고 관련제도 등이 수반되어야 할 것이다. 본 연구는 해양수산부의 친수공간개발사업이 실시된 이래 전국의 유인등대가 보다 더 적절하게 활용되기 위해 다각도로 검토되어야 할 논제들을 밝히고자 의도하였다.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2016년도 춘계학술대회
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• pp.18-19
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• 2016

현재, 우리나라 전 해상에서는 38개소의 유인등대가 운영 중이다. 사람이 거주하면서 등대를 관리하는 유인등대는 항로표지로서의 기능강화와 관리체계 효율화 방안을 마련하기 위해 변화가 불가피하다. 이들 유인등대 중 무인화 등대에 부합하는 등대를 선정하기 위한 유인등대 관리체계 개선 기준(항로표지 기능, 광역관리 기능성, 국가정책, 해양문화공간 운영)을 마련하여 무인등대화를 위한 평가자료를 수집 및 분석하여 AHP 가중치 및 리커드 척도(Likert Scale) 분석 기법을 적용한 결과를 토대로 관할청에서 멀리 떨어진 항로표지의 기능장애시 복구시간을 단축하기 위해 유인등대의 역할과 기능을 강화할 수 있고, 주변의 항로표지를 소권역화 할 수 있는 거점등대를 선정하였다. 이에 본 논문은 우리나라 전 해상에 산재되어 있는 항로표지시설들을 효과적으로 관리 및 유인등대의 생활환경 개선에 도움이 될 것으로 판단된다.

• ALGAE
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• 제30권3호
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• pp.183-195
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• 2015

The dinoflagellate genus Pelagodinium is genetically classified in distinct sub-clades and subgroups. However, it is difficult to determine whether this genetic diversity represents intra- or interspecific divergence within the genus since only the morphology of the type strain of the genus Pelagodinium, Pelagodinium bei, is available. An isolate associated with the genus Pelagodinium from Shiwha Bay, Korea, was recently cultured. This isolate was clustered with 3 to 4 strains from the Atlantic Ocean, Mediterranean Sea, and Indian Ocean. This cluster was distinct from the subgroup more closely associated with P. bei. The morphology of the isolate was analyzed using optical and scanning electron microscopy and was almost identical to that of P. bei except that this isolate had two series of amphiesmal vesicles (AVs) in the cingulum, unlike P. bei that has one series. When the pigment compositions of the isolate and P. bei were analyzed using high-performance liquid chromatography, these two strains had peridinin as a major accessory pigment and their pigment compositions were almost identical. In addition, the swimming behaviors of these two strains were very similar. The reexamination of the type culture of P. bei revealed two series in the cingulum as for the isolate. The new findings on the number of series of AVs in the cingulum, the pigment composition, and the swimming behaviors suggest that P. bei and the isolate are conspecific despite their genetic divergence. This study provides a basis to further understand the molecular classification within Pelagodinium combining genetic, morphological, pigment, and behavioral data.

• 한국패류학회지
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• 제19권1호
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• pp.33-40
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• 2003

To obtain the basic information on culture conditions for the larvae of Saxidomus purpuratus, experiments were conducted on the population from southern coast for (1) the success in fertilization and development from artificial fertilization among different months of a year, (2) the viability of sperms after exposure to seawater, (3) and the effects of temperature, salinity, and food organism on the survival and growth of larvae. Gametes obtained from dissection showed high rate of fertilization at all months. But the rate of development was higher only May-July. Developmental success seemed to be related with the quality of eggs at the time of fertilization. Developmental times for 2-cell, 4-cell, 8-cell, blastula, trochophore larva, and veliger larva at 20$^{\circ}C$ were 1.5, 2, 4, 18, 24, and 32 hr, respectively. Sperms could survive for more than 8 hr, however, actively swimming sperms could be found within 1 hr after exposure to seawater. It is recommended that sperms should be used for fertilization as soon as possible when they are exposed to seawater. At temperature of 35$^{\circ}C$, all the larvae died during 48 hr. Larval survival decreased when salinity was either lower than 20 psu or higher than 40 psu, and was 0% when salinity was 10 psu. Optimal range of temperature and salinity for rearing larvae of S. purpuratus were 20-25$^{\circ}C$ and 20-40 psu, respectively. Larvae grew from 111.5 to 235.3 ${\mu}$m during 21 days. Larvae fed mixed diets grew faster than unialgal diets. The fastest growth was observed when larvae were fed on the mixture of Isochrysis galbana and Nannochloris oculata.

• 한국콘텐츠학회논문지
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• 제13권2호
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• pp.127-136
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• 2013

본 연구의 목적은 캔 아트 문화 사업화를 위해 필요한 성공요인을 계층분석 연구방법론을 활용하여 도출하고, 캔 아트 문화사업화의 블루오션 전략을 제시 하였다. 연구결과는 다음과 같다. 첫째, 가장 중요하다고 판단된 캔 아트의 문화사업화 성공요인은 Eco 상품화(0.227), 문화적 가치(0.223), 유통(0.217), 체험학습(0.202) 순으로 나타났다. 둘째, 캔 아트 산업이 성공하기 위해 중요한 세부 중요 요인은 Eco디자인(0.118), 재활용 인식개선(0.118), 교육적 가치 증대(0.117), Viral 마케팅(0.113), 정기적인 체험학습(0.105) 순으로 고려되었다. 그리고 본 연구시사점은 캔 아트를 블루오션으로서 창조해야 할 전략은 환경 공예교육으로 심화 시키고, Eco 상품화에 주력하는 것으로 제시하고 있다.

• 수산해양기술연구
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• 제50권3호
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• pp.385-395
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• 2014

This study is one of the research series for providing basic data that would contribute to the development of the field of fishing industry through the systematic fishing management, protection of fishery resources and healthy fishing culture with safety measures for fishermen in Jeju island according to the implementation of "Fishing Management and Support Act". As a result of analyzing the related acts, the contents of this study are related with provisions of "Fishing management and Support Act" on acts on fishing management, fish bait management, support for fishing and fishing related industries and with provisions of "Act of the preservation and management of desert island" on the preservation, use and development of desert island (use and development in a desert island, behavior in usable desert island). As a result of the survey, the rocks on the seashore showed the highest popularity with 52.6% for fishermen and the seawall was the highest with 47.1% for Maritime police as their fishing place in the fishing related activity data. And as the survey result about the awareness of "Fishing management and Support Act", 65.2% of general fishermen, 36.0% of maritime police and 70.5% of fishing related agencies answered that they knew the act. It was also surveyed whether they consider its contribution to fishing related industry development and positive result was shown by general fishermen and maritime police with 46.8%, 48.0% respectively whereas fishing-related enterprises showed negative answer with 47.7%. In the survey of fishing related regulation standard, general fishermen, maritime police and fishing related agencies all showed different opinions regarding the regulation standard. It is recommended that regulation standard needs to be set up after detailed review.

• 수산해양기술연구
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• 제59권2호
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• pp.164-171
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• 2023

This study analyzed the flow inside floating seedling equipment for Scapharca subcrenata. Due to the aging society of fishing villages, it is impossible to continuously input the labor force. Therefore, it is necessary to improve efficiency. Scapharca subcrenata has high per capita consumption. It serves as an important aquatic food resource. Scapharca subcrenata culture tends to be highly dependent on the natural environment. Production of Scapharca subcrenata is difficult to predict with low stability. In the past, manpower directly installed bamboo nets in mudflats. The seedling equipment devised in this study is a floating type and can be freely moved on the sea according to the prediction of Scapharca subcrenata generation. The flow around the floating seedling equipment was analyzed by numerical analysis. The physical phenomena of the flow around the net inside the floating seedling equipment were visualized. As a result, the space between the floating seedling equipment and the bottom net and the space between the net groups showed a lower flow rate than the inlet flow rate. It is expected that the low flow rate of the floating seedling equipment will have a positive effect on the attachment of Scapharca subcrenata.

• 환경생물
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• 제40권3호
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• pp.275-280
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• 2022

부유물질은 수생생물의 성장과 생존에 중요한 역할을 한다. 본 논문은 cyst에서 부화한 Artemia 유생(branchiopoda), 조간대에서 채집한 T. west (copepod)와 H. koreanus (amphipoda)를 사용하여 96시간 동안 부유물질에 노출된 생물의 생존 영향을 규명하였다. 실험 결과 Artemia 유생과 T. west의 생존율은 50.0mg L^-1 이상의 부유물질 농도에 영향을 받지 않으나 H. koreanus는 부유물질 농도가 증가함에 따라 낮은 생존율을 보였다(p<0.05). 결론적으로 50.0mg L^-1 이상의 부유물질에 지속적으로 노출된 Artemia 유생과 T. west는 미세한 생태 스트레스의 영향을 받지만 H. koreanus는 생존 가능한 내성범위를 벗어나 사망한 개체가 증가할 것으로 판단된다.

• ALGAE
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• 제37권2호
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• pp.105-121
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• 2022

Modern seaweed farming relies heavily on seedlings from natural beds or vegetative cuttings from previous harvests. However, this farming method has some disadvantages, such as physiological variation in the seed stock and decreased genetic variability, which reduces the growth rate, carrageenan yield, and gel strength of the seaweeds. A new method of seedling production that is sustainable, scalable, and produces a large number of high-quality plantlets is needed to support the seaweed farming industry. Recent use of tissue culture and micropropagation techniques in eucheumatoid seaweed production has yielded promising results in increasing seed supply and growing uniform seedlings in large numbers in a shorter time. Several seaweed species have been successfully cultured and regenerated into new plantlets in laboratories using direct regeneration, callus culture, and protoplast culture. The use of biostimulants and plant growth regulators in culture media increases the seedling quality even further. Seedlings produced by micropropagation grew faster and had better biochemical properties than conventionally cultivated seedlings. Before being transferred to a land-based grow-out system or ocean nets for farming, tissue-cultured seedlings were recommended to undergo an acclimatization process to increase their survival rate. Regular monitoring is needed to prevent disease and pest infestations and grazing by herbivorous fish and turtles during the farming process. The current review discusses recent techniques for producing eucheumatoid and other valuable seaweed farming materials, emphasizing the efficiency of micropropagation and the transition from laboratory culture to cultivation in land-based or open-sea grow-out systems to elucidate optimal conditions for sustainable seaweed production.