• Clinical and Experimental Reproductive Medicine
• /
• 제45권1호
• /
• pp.17-24
• /
• 2018

Objective: To investigate sperm chromatin/DNA integrity, global DNA methylation, and DNMT mRNA transcription in men with oligoasthenoteratozoospermia (OAT) compared with normozoospermic men. Methods: Semen samples from 32 OAT patients who comprised the case group and 32 normozoospermic men who comprised the control group were isolated and purified using a standard gradient isolation procedure according to World Health Organization criteria. DNMT1, DNMT3A, and DNMT3B transcripts were then compared between groups using real-time quantitative reverse-transcription polymerase chain reaction. Global DNA methylation in sperm was determined by an enzyme-linked immunosorbent assay. Protamine deficiency and the proportion of apoptotic spermatozoa were evaluated using chromomycin A3 (CMA3), aniline blue (AB), and toluidine blue (TB) staining, as well as the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The p-values < 0.05 were considered to indicate statistical significance. Results: Significantly higher proportions of AB+, TB+, CMA3+, and TUNEL+ spermatozoa, as well as DNMT3A and DNMT3B transcription, were found in the OAT group. Positive correlations were detected between sperm parameters, DNA/chromatin damage, and DNMT3A and DNMT3B transcripts. Global DNA methylation was significantly higher in the OAT patients and had a significant correlation with abnormal results of all sperm chromatin integrity tests, but was not associated with DNMT1, DNMT3A, or DNMT3B expression. Conclusion: Oligoasthenoteratozoospermic men showed abnormal sperm parameters, abnormal chromatin/DNA integrity, and a higher global DNA methylation rate, as well as overexpression of DNMT mRNA.

• Journal of Ginseng Research
• /
• 제42권3호
• /
• pp.370-378
• /
• 2018

Background: Ginseng has been the subject of many experimental and clinical studies to uncover the diverse biological activities of its constituent compounds. It is a traditional medicine that has been used for its immunostimulatory, antithrombotic, antioxidative, anti-inflammatory, and anticancer effects. Ginseng may interact with concomitant medications and alter metabolism and/or drug transport, which may alter the known efficacy and safety of a drug; thus, the role of ginseng may be controversial when taken with other medications. Methods: We extensively assessed the effects of Korean Red Ginseng (KRG) in rats on the expression of enzymes responsible for drug metabolism [cytochrome p450 (CYP)] and transporters [multiple drug resistance (MDR) and organic anion transporter (OAT)] in vitro and on the pharmacokinetics of two probe drugs, midazolam and fexofenadine, after a 2-wk repeated administration of KRG at different doses. Results: The results showed that 30 mg/kg KRG significantly increased the expression level of CYP3A11 protein in the liver and 100 mg/kg KRG increased both the mRNA and protein expression of OAT1 in the kidney. Additionally, KRG significantly increased the mRNA and protein expression of OAT1, OAT3, and MDR1 in the liver. Although there were no significant changes in the metabolism of midazolam to its major metabolite, 1'-hydroxymidazolam, KRG significantly decreased the systemic exposure of fexofenadine in a dose-dependent manner. Conclusion: Because KRG is used as a health supplement, there is a risk of KRG overdose; thus, a clinical trial of high doses would be useful. The use of KRG in combination with P-glycoprotein substrate drugs should also be carefully monitored.

• Clinical and Experimental Reproductive Medicine
• /
• 제46권3호
• /
• pp.112-118
• /
• 2019

Objective: Oxidative stress contributes to male infertility, and antioxidants have been recommended for treating idiopathic oligoasthenoteratozoospermia (OAT). There is, however, a lack of agreement on the type, dosing, and use of individual antioxidants or combinations thereof. The purpose of this study was to compare the effects of two doses of coenzyme Q10 (CoQ10) on semen parameters and antioxidant status in men with idiopathic OAT. Methods: In this prospective study, patients with idiopathic OAT received 200 mg/day (n = 35) or 400 mg/day (n = 30) of CoQ10 orally for 3 months. All patients underwent semen analysis according to the fifth editions of the World Health Organization criteria. Total antioxidant capacity (TAC), catalase (CAT) activity, and superoxide dismutase (SOD) activity were measured both before and after treatment. Results: Treatment with CoQ10 (200 mg/day or 400 mg/day) resulted in a significant increase in sperm concentration from baseline ($8.22{\pm}6.88$ to $12.53{\pm}8.11million/mL$, p= 0.019; $7.58{\pm}5.41$ to $12.33{\pm}6.1million/mL$, p= 0.002, respectively), progressive motility ($16.54%{\pm}9.26%$ to $22.58%{\pm}10.15%$, p=0.011; $14.22%{\pm}12.85%$ to $26.1%{\pm}14.52%$, p= 0.001, respectively), and total motility ($25.68%{\pm}6.41%$ to $29.96%{\pm}8.09%$, p= 0.016; $23.46%{\pm}12.59%$ to $34.82%{\pm}14.17%$, p= 0.001, respectively). CoQ10 therapy also increased TAC (p= 0.009, p= 0.001, respectively), SOD activity (p= 0.004, p= 0.001, respectively), and CAT activity (p= 0.039, p= 0.024, respectively). Furthermore, antioxidant measures correlated significantly with seminal fluid parameters (r = 0.36-0.76). Conclusion: CoQ10 supplementation improved semen parameters and antioxidant status in men with idiopathic OAT, with a greater improvement shown in men who took 400 mg/day than in those who took 200 mg/day.

• 농업과학연구
• /
• 제47권4호
• /
• pp.941-949
• /
• 2020

This study evaluated the nutritional value of Italian ryegrass (IRG) as a forage source for Hanwoo. The nutritional value of IRG was assessed and compared to that of rice straw, oat hay, and timothy hay using two different methods: 1) in vitro ruminal fermentation 2) in vivo total tract digestibility. In vitro DM digestibility was lower in rice straw compared to the other three forage sources after both 24 and 48 h of incubation (p < 0.01). Among the four forage sources, IRG had a higher NH3-N concentration after both 24 and 48 h of incubation (p < 0.01). In the in vivo digestibility trials, four different substrates were used: 1) 80% concentrate with 20% rice straw, 2) 80% concentrate with 20% oat hay, 3) 80% concentrate with 20% IRG, and 4) 80% concentrate with 20% timothy hay. The dry matter, crude protein, non-fiber carbohydrate, and detergent fiber digestibility were the greatest in the C80-IRG20 among the four forage groups. In summary, IRG had a similar level of energy efficiency compared to oat hay and timothy hay. Furthermore, the result of the chemical composition analysis showing a higher ammonia concentration in the in vitro fermentation experiment and the high protein digestibility in the in vivo experiment indicate that IRG is a good source of protein compared to oat hay and timothy hay.

• Clinical and Experimental Reproductive Medicine
• /
• 제48권1호
• /
• pp.50-60
• /
• 2021

Objective: This study investigated the mRNA expression of gamma-aminobutyric acid (GABA) receptors in the sperm of oligoasthenoteratozoospermic (OAT) and teratozoospermic (TER) men compared to normozoospermic (NOR) men, as well as the relationships between GABA receptor expression and sperm parameters, fertilization rate, and embryo quality. Methods: The mRNA expression of GABA A-α1 and GABA B-R2 receptors in sperm was examined using reverse transcription-polymerase chain reaction in three groups of patients: NOR (n=32), OAT (n=22), and TER (n=45). The fertilization rate and embryo quality were assessed in 35 patients undergoing intracytoplasmic sperm injection (ICSI; 10 NOR, 10 OAT, and 15 TER men). Results: OAT men had significantly higher mRNA expression of GABA A-α1 and GABA B-R2 receptors in sperm than NOR men; however, the difference between TER and NOR men was not significant. High levels of these receptors were significantly correlated with low sperm concentration, motility, and morphology, as well as the rate of good-quality embryos (GQEs) at the cleavage stage after ICSI. Patients whose female partners had a >50% GQE rate at the cleavage stage had significantly lower levels of GABA A-α1 receptor expression than those whose partners had a ≤50% GQE rate. Conclusion: Our findings indicate that mRNA levels of GABA receptors in human sperm are correlated with poor sperm quality and associated with embryo development after ICSI treatment. The GABA A-α1 receptor in sperm has a stronger relationship with embryo quality at the cleavage stage than the GABA B-R2 receptor.

• Journal of Animal Science and Technology
• /
• 제63권1호
• /
• pp.137-148
• /
• 2021

The effect of the addition of dietary fiber extracted from wheat, bamboo, and oat on the quality of fried pork loin batter was investigated. Quality evaluation included proximate composition, pH, color, viscosity, coating and frying yield, electronic nose, and sensory evaluation. Regarding proximate composition of fried batter and fried pork loin, the water content of the dietary fiber treatments was significantly higher than that of the control (p < 0.05), whereas fat content was significantly lower than that of the control (p < 0.05). The lightness of non-fried batter with dietary fiber treatments was significantly higher than that of the control (p < 0.05), whereas the yellowness was significantly lower than that of the control (p < 0.05). The lightness, redness, and yellowness of fried pork loin with dietary fiber treatment were significantly lower than those of the control (p < 0.05). The viscosity and coating and frying yield of dietary fiber treatments were significantly higher than those of the control (p < 0.05). The volatile compounds of dietary fiber treatments were decreased "tallowy" flavor and increased "buttery" and "milky" flavor. The principal components of bamboo and oat fiber treatments were clearly distinguishable from those of the control; however, similar principal components as those of the control were obtained with wheat fiber treatment. Regarding sensory evaluation, the color, texture, and overall acceptability of wheat and oat fiber treatments were significantly higher than those of the control (p < 0.05), and the flavor of the wheat fiber treatment was significantly higher than that of the control (p < 0.05). These results show that wheat and oat fibers are suitable for fried pork loin batter and improve its quality.

• 한국토양비료학회지
• /
• 제42권6호
• /
• pp.415-422
• /
• 2009

간척조성지에서 녹비 사료작물의 연중 재배기술개발을 위하여 화옹간척지, 이원간척지 및 영산강간척지에 시험포장을 설치하여 하계 녹비 사료작물인 수단그라스, 세스바니아, 제주재래피의 후작으로 동계 녹비 사료작물인 보리, 호밀 및 귀리를 공시하여 시험재배하였다. 토양염농도와 공간변이성은 이원간척지와 화옹간척지가 시험작물의 고사나 생육저해를 유발할 정도로 매우 높은 반면에 영산강간척지는 작물의 생육저해를 유발하지 않을 정도로 매우 낮았다. 시험작물의 파종 후 출현개체수는 염농도의 증가에 따라 로그함수적으로 감소하여 최고 출현개체수의 50%로 감소하는 EC($dS\;m^{-1}$)가 보리 6.5, 호밀과 귀리 5.1로 추정되어 토양염농도가 평균 $6dS\;m^{-1}$을 초과할 경우 맥류의 출현이 크게 감소하였다. 시험작물의 수량은 염농도의 증가에 따라 로그함수적으로 감소하여 청예수량이 50% 이상 감수되는 염농도($dS\;m^{-1}$)는 보리 4.9, 호밀 6.2, 귀리 6.0으로 추정되고, 건물수량은 보리 5.5, 호밀 6.2, 귀리 5.8로 추정되며, 종실수량은 보리 5.6, 호밀 5.8, 귀리 5.7로 추정되어 동작 맥류재배를 위해서는 적어도 $6dS\;m^{-1}$ 이하로 염농도가 관리되어야 할 것으로 생각되었다. 이원과 화옹간척지는 염농도가 $10dS\;m^{-1}$ 이상으로 높아서 출현부진과 생육저해 및 고사가 많아서 수확이 불가능하였으나 영산강간척지는 염농도가 $6.17dS\; m^{-1}$ 이하로 유지되어 염에 의한 피해가 발생하지 않아서 청예수량($kg\;10a^{-1}$)은 호밀 1,479, 귀리 1,328, 보리 1,582이었고, 건물수량($kg \;10a^{-1}$)은 호밀 689, 귀리 683, 보리 701이었으며, 종실수량($kg \;10a^{-1}$)은 보리가 395, 호밀이 164, 귀리가 325를 얻었다.

• 식물병연구
• /
• 제25권4호
• /
• pp.157-163
• /
• 2019

본 연구에서는 귀리에서 독성곰팡이 발생현황을 평가하기 위해 2017년 2018년 4월부터 6월까지 귀리 재배지 강진, 정읍을 정기적으로 모니터링 하였다. 총 745개의 곰팡이 균주를 형태학적 방법으로 분리한 후 마커 유전자 염기서열을 분석하여 동정하였다. 분리된 곰팡이의 약 92%가 Fusarium속 균주였고, Penicillium속(5.9%)과 Aspergillus속(2.1%) 순으로 분리되었다. Fusarium속 균주의 대부분이 F. asiaticum (83.1%)이었고, F. incarnatum (5.4%), F. proliferatum (3.5%), F. fujikuroi (2.8%), F. tricinctum species complex (FTSC) 11 (1.5%), F. graminearum (1.0%) 순으로 많이 분리되었다. F. asiaticum의 약 97%가 nivalenol 독소화학형이 있었고, 3-acetyl deoxynivalenol (3.2%) 독소화학형과 15-acetyl deoxynivalenol (0.4%) 독소화학형도 발견되었다. 선발된 Fusarium 균들의 병원성 실험결과 F. asiaticum이 실험에 사용된 모든 식물체에서 다양한 범위의 병원성을 나타내었다. 병증이 있는 종실에서 분리된 F. graminearum and FTSC 11 균주들은 쌀귀리에서 강한 병원성을 나타내었다. 또한 FTSC 11 균주를 제외한 모든 균주들이 쌀배지에서 nivalenol (0.2-7.6 ㎍/g), deoxynivalenol (0.03-6.1 ㎍/g), zearalenone (0.1-27.0 ㎍/g)을 생성하였다. 본 연구는 국내에서는 처음 보고되는 F. asiaticum에 의한 귀리의 붉은 곰팡이병 사례이다. 본 연구의 결과는 밀, 보리, 벼와 마찬가지로 F. asiaticum이국내귀리에서우점종임을보여준다.

• 한국식품영양과학회지
• /
• 제44권11호
• /
• pp.1660-1665
• /
• 2015

본 연구에서는 국내에서 개발된 귀리 품종의 항산화 성분 및 항산화 활성을 측정하고 항산화 성분과 활성 간의 상관관계를 분석하고자 하였다. 항산화 성분으로는 총 폴리페놀과 vitamin E 함량을 측정하였으며 항산화 활성은 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS) 및 1,1-diphenyl-2-picrylhydrazyl(DPPH) 라디칼 제거능, 환원력, 금속이온 제거능을 측정하였다. 총 폴리페놀 함량은 83.41~130.59 mg/100 g으로 나타났으며 쌀귀리 품종의 총 폴리페놀 함량이 겉귀리보다 높은 것으로 나타났다. 또한 vitamin E 함량은 0.54~1.90 mg/100 g으로 나타났으며 중모 2005 품종을 제외한 모든 품종의 귀리에서 ${\alpha}$-tocotrienol의 비중이 가장 높은 것으로 나타났다. ABTS 라디칼 제거능, DPPH 라디칼 제거능 및 환원력은 대양 품종이 각각 116.14 mg TEAC/100 g, 38.58 mg TEAC/100 g 및 $A_{700}=0.922$로 가장 높은 활성을 보였다. 항산화 성분과 활성 사이의 상관분석 결과 총 폴리페놀 함량과 ABTS 라디칼 제거능(0.847, P<0.01), DPPH 라디칼 제거능(0.887, P<0.01) 및 환원력(0.653, P<0.01) 사이에 높은 상관성을 나타내어 귀리 메탄올 추출물의 항산화 활성에 기여하는 성분은 폴리페놀성 화합물인 것으로 사료된다. 본 연구 결과는 귀리의 항산화 성분과 항산화 활성 연구에 있어 기초자료로서 활용될 수 있을 것으로 생각하며, 국내 개발 귀리 품종의 이용 증진에 영향을 끼칠 것으로 기대한다.

• 한국초지조사료학회지
• /
• 제29권2호
• /
• pp.111-120
• /
• 2009

조사료 생산에 유망한 동계작물중 보리, 밀, 호밀, 귀리, 트리티케일(라이밀) 등 5종의 총체맥류에 대하여 월동 후 예취시기에 따른 생육 및 조사료 생산성에 관한 생장곡선을 조사하기 위하여 충남 예산군 소재의 충남농업기술원 답리작 포장에서 2006${\sim}$2007년에 수행한 결과는 다음과 같다. 지상부 건물중에서 줄기가 차지하는 비율은 월동 후 점차 증가하다가 어느 정점부터는 감소하는 2차원적인 경향을 보였는데, 줄기의 구성비율이 최고가 되는 시기는 각각 영양 보리 5월 1일, 금강 밀 5월 5일, 곡우 호밀 5월 10일, 신영 트리티케일 5월 11일, 삼한 귀리 5월 13일경이었다. 잎은 월동 후 성숙이 진행될수록 직선적으로 감소하는 경향을 보였다. 이삭이 차지하는 비율은 영양 보리가 출수 후 직선적으로 증가하였는데 밀, 트리티케일, 귀리에 비하여 높았고, 호밀이 가장 낮았다. 영양 보리가 출수기 이후에 다른 총체맥류에 비하여 줄기의 구성비율이 낮고 이삭과 잎의 구성비율이 높은 전형적인 총체맥류에 속하였고, 다음은 밀과 귀리이었으며, 호밀은 알곡 비율이 적은 조사료 작물에 해당하였다. 사일리지 제조에 알맞은 수분 함량(60${\sim}$ 70%에 도달하는 시기는 영양 보리 5월 25일, 금강 밀 5월 25일${\sim}$6월 5일, 삼한 귀리 6월 5일이었고, 이 때 생체수량은 곡우호밀>삼한 귀리>신영 트리티케일>영양 보리>금강 밀 순으로 높았다. 생체수량은 월동 후 생육이 진전됨에 따라 증가하다가 일정시기 이후 감소하는 경향을 보였는데, 중부지역에서 생체수량이 최대로 되는 시기는 영양 보리 5월 7일경, 금강 밀 5월 14경, 곡우 호밀 5월 9일, 신영 트리티케일 5월 17일경, 삼한 귀리 5월 말이었다. 건물수량은 4종의 총체맥류 모두 월동 후 성숙기까지 직선적으로 증가하는 양상을 보였고, 사일리지 제조에 알맞은 수분 함량에 도달하는 시기에 조사한 건물수량은 귀리 > 보리 > 밀 > 트리티케일 > 호밀 순으로 높았다. 상대 생장률은 월동 후 서서히 증가하다가 정점 이후로 감소하는 2차원적인 생장을 보였는데, 월동 후 예취시기가 빠를수록 곡우 호밀이 높은 상대생장률을 보였고, 예취시기가 늦을수록 삼한 귀리가 높았으며, 금강 밀, 영양 보리, 신영 트리티케일은 비슷한 상대생장률을 보였다.