• Title/Summary/Keyword: Oak wilt disease

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Isolation and Identification of Entomopathogenic Fungus from the Pine Wilt Disease Vector, Monochamus alternatus Hope(Coleoptera: Cerambycidae) in Korea

  • Shin, Tae-Young;Choi, Jae-Bang;Bae, Sung-Min;Cha, Ye-Rim;Oh, Jeong-Mi;Koo, Hyun-Na;Woo, Soo-Dong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.18 no.2
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    • pp.125-129
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    • 2009
  • Entomopathogenic fungi were isolated directly from a cadaver of adult Monochamus alternatus supporting fungal sporulation, using a semi-selective medium and then screened several fungal colonies. The pathogenicity of each fungus was tested using oak longicorn beetle, Moechotypa diphysis, as substitutive insect. As the result, only one of them showed high pathogenicity against M. diphysis, with up to 100% mortality within 21 days of inoculation. Selected fungus was named as MaW1 and identified by Beauveria bassiana using microscopic examination and DNA analysis. Pathogenicity was also evaluated to M. alternatus.

Attractant Effect of Trap Logs Treated with Ethanol to Platypus koryoensis (Coleoptera: Platypodidae) (에탄올 처리에 의한 광릉긴나무좀(Platypus koryoensis) 유인목 효율 증대)

  • Son, Su-Yeon;Lee, Sun Keun;Seo, Sang-Tae
    • Korean journal of applied entomology
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    • v.54 no.4
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    • pp.443-448
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    • 2015
  • The attractant effect to Platypus koryoensis (Murayama), an insect vector of Korean oak wilt disease, was determined using a multi-funnel trap and trap logs treated with ethanol and ethanol extract of the bark of the Mongolian oak Quercus mongolica (Fisher). The number of entrance holes in ethanol and bark ethanol extract treatments was higher than that in the control (blank bottle). However, the number of entrance holes in ethanol and bark ethanol extract treatments was not significantly different. The number of entrance holes in the logs increased with increase in diameter of the logs. The lower part of the trap logs had significantly more number of entrance holes than that in the upper part (p < 0.05). The lower part of trap logs tends to be rarely exposed to direct sunlight resulting in desiccation of the logs, which in turn provides good conditions for microbial growth, a food source for P. koryoensis. The results show, that ethanol could be used effectively in the monitoring and maintenance of P. koryoensis because of the cheaper cost of ethanol than bark ethanol extracts, and thus, having a higher economical advantage. Further research of oak bark ethanol extracts is necessary to improve its efficiency.

Ecological Characteristics and Changes in Plant Community Structure in Mt. Cheongryang, Incheon (인천시 청량산의 생태적 특성과 식생구조의 변화)

  • Lee, Sang-Hee;Han, Bong-Ho;Park, Seok-Cheol
    • Journal of the Korean Institute of Landscape Architecture
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    • v.49 no.2
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    • pp.74-88
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    • 2021
  • In the present study, the characteristics and changes in the vegetation of Mt. Cheongryang, Inchon, were examined to identify and determine appropriate ways to restore the health of the urban forest and to preserve its vegetation. The vegetation of the community of Quercus mongolica (Mongolian oak) on Mt. Cheongryang appeared to decrease in response to the control of the wilt disease of oak trees. The communities of Sorbus alnifolia (Korean mountain ash) and Styrax japonicus (Snowbell tree) have increased. Pinus rigida (Pitch Pine) had its overall territory decrease, but the current state of the Pinus rigida (Pitch Pine) was estimated to be stable due to its dominance as a tree layer species. In regards to Robinia pseudoacacia (Black Locust), the urbanized species of Sorbus alnifolia (Korean mountain ash), and Styrax japonicus (Snowbell tree), their areas have increased with the appearance of Magnolia obovate (Whiteleaf Japanese Magnolia). The biodiversity of Mt. Cheongryang has decreased by simplifying species in the tree layer and understory species thereof, and the initial success of species in marginal areas has increased. The absence of potential succession was attributed to the termination of ecological succession; thereby, the current vegetation structure was concluded to be remaining as it is for the time being. Soil texture in the mountain primarily consisted of sandy loam or loamy sand; the pH of the soil was in the range 4.26-4.86, rendering a mean pH of 4.59. The content of organic matter (O.M.) appeared having a distributing range of 2.18-9.60%, rendering a mean value of 4.33%. To promote species diversity, several methods are suggested, such as prevention of soil acidification, selecting nationally-grown trees from moist soil or valleys for afforestation, preventing species appearing due to urbanization or excessive growth, protecting the understory vegetation and species with hygropreference, and managing the forest to maintain a multi-layered vegetation structure.

Characteristics of Vegetation Structure of Managed Area by Oak Wilt Disease in Bukhansan National Park (북한산국립공원 참나무시들음병 방제지역 식생구조 특성 연구)

  • Han, Bong-Ho;Choi, Jin-Woo;Yeum, Jung-Hun;Lee, Kyong-Jae
    • Korean Journal of Environment and Ecology
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    • v.28 no.3
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    • pp.342-356
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    • 2014
  • This study aims to provide the fundamental data for restoration through the analysis of vegetation structure characteristic for managed area with deforest and fumigation among the places damaged by oak wilt disease in Bukhansan National Park. Study areas were selected in six damaged areas with 18 plots ($20m{\times}20m$) including comparing plots between year 2006 and year 2011 after preliminary study finding the exact location with easy access. Analysis items are density and coverage of under story layer, Importance Percentage of under story layer and Importance Percentage of urban adapted species and alien species. As a result, density and coverage under the canopy layer's vegetation was higher than comparing plot in Musugol valley and Soquicheon valley which 4 years and more passed. And Wondobong, Darakwon, Doseonsa and Dobong valley which had not been passed 3 years after deforestation and fumigation decreased in those of vegetation. However, the plots in Wondobong and Doseonsa was analyzed to be higher in density of shrubs than comparing plot. In terms of distirbution of species in under canopy layer which is grown as a upper vegetation, Quercus serrata and Sorbus alnifolia dominated each other in under canopy layer except for Wondobong and Darakwon. Wondobong continuously maintained Quercus mongolica and Q. mongolica and Pinus densiflora dominated in under canopy layer of Darakwon. S. alnifolia which is Urban adapted species were distributed in most study areas. In terms of alien species, Magnolia obovata and Robinia pseudoacacia were newly intruded in the opened crown, and Pueraria thunbergiana was distributed in Soquicheon valley. As the result of soil analysis, acidification(pH 4.49) and low level of organic matter(3.23%), available phosphate(1.40mg/kg) and exchangeable cation were analyzed and it was considered that those environment poorly support the vegetation growth.

Antifungal Property of Microorganisms against Korea Oak Wilt Pathogen, Raffaelea quercus-mongolicae (참나무시들음 병원균 Raffaelea quercus-mongolicae에 대한 항균미생물 분리)

  • Lee, Sang-Hyun;Lee, Seung-Kyu;Kim, Jae-Young;Lee, Chong-Kyu;Kim, Kyung-Hee;Yi, Yong-Sub
    • Microbiology and Biotechnology Letters
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    • v.40 no.1
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    • pp.66-69
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    • 2012
  • Five strains out of 200 candidate strains (SG 1-9, 1-12, SG 2-8, 2-10, and 2-17) were selected to determine their antifungal activity against Raffaelea quercus-mongolicae. The 16S rDNA sequences of the five strains were determined by sequencing analysis and analyzed by the homology of the blast program at NCBI. The homology search showed that SG 1-9 and 1-12 had a 98% homology with Streptomyces cinnamoneus and 98% homology with Burkholderia cepacia, while SG 2-8, 2-10, and 2-17 had a 99% homology with Streptomyces fradiae, a 97% homology with Staphylococcus epidermidis, and a 99% homology with Staphylococcus epidermidis. Out of the five selected strains, organic extract and protein extracts of SG2-17 strain broth were employed to determine antifungal activity against Raffaelea quercus-mongolicae. The organic extract exhibited antifungal activity, but the protein extracts did not demonstrate such an activity. Three organic solvents, butanol, benzene, and ethyl acetate, were also used for determination of antifungal activities. The activity measurements revealed that benzene extract possessed the greatest inhibitory effect on the growth of Raffaelea quercus-mongolicae, with the next highest being butanol extract, and ethyl acetate extract being the lowest.