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Antifungal Property of Microorganisms against Korea Oak Wilt Pathogen, Raffaelea quercus-mongolicae

참나무시들음 병원균 Raffaelea quercus-mongolicae에 대한 항균미생물 분리

  • 이상현 (국립산림과학원 병해충과) ;
  • 이승규 (국립산림과학원 병해충과) ;
  • 김재영 (호서대학교 생화학과) ;
  • 이총규 (진주대학교 산림자원학과) ;
  • 김경희 (국립산림과학원 병해충과) ;
  • 이용섭 (호서대학교 생화학과)
  • Received : 2011.10.17
  • Accepted : 2011.12.09
  • Published : 2012.03.28

Abstract

Five strains out of 200 candidate strains (SG 1-9, 1-12, SG 2-8, 2-10, and 2-17) were selected to determine their antifungal activity against Raffaelea quercus-mongolicae. The 16S rDNA sequences of the five strains were determined by sequencing analysis and analyzed by the homology of the blast program at NCBI. The homology search showed that SG 1-9 and 1-12 had a 98% homology with Streptomyces cinnamoneus and 98% homology with Burkholderia cepacia, while SG 2-8, 2-10, and 2-17 had a 99% homology with Streptomyces fradiae, a 97% homology with Staphylococcus epidermidis, and a 99% homology with Staphylococcus epidermidis. Out of the five selected strains, organic extract and protein extracts of SG2-17 strain broth were employed to determine antifungal activity against Raffaelea quercus-mongolicae. The organic extract exhibited antifungal activity, but the protein extracts did not demonstrate such an activity. Three organic solvents, butanol, benzene, and ethyl acetate, were also used for determination of antifungal activities. The activity measurements revealed that benzene extract possessed the greatest inhibitory effect on the growth of Raffaelea quercus-mongolicae, with the next highest being butanol extract, and ethyl acetate extract being the lowest.

Raffaelea quercus-mongolicae에 대한 항균작용을 하는 미생물을 분리하기 위하여 200개의 균주를 분리하였으며 이 중 SG 1-9, 1-12와 SG 2-8, 2-10, 2-17 5개의 균주에서 항균활성을 확인하였다. 5개의 균주는 확인을 위하여 16S rDNA 염기서열 분석을 하였으며, SG 1-9는 Streptomyces cinnamoneus와 98%의 homology가 SG 1-12는 Burkholderia cepacia와 98% homology가 같은 것으로 나타났다. 또한 SG 2-8은 Streptomyces fradiae와 99%의 homology를 SG 2-10은 Staphylococcus epidermidis와 97%를 SG 2-17은 Staphylococcus epidermidis와 99%의 homology를 나타내었다. 위 5개의 균주 중 활성이 가장 강한 SG 2-17의 유기용매추출물과 단백질추출물을 분리하여 활성을 조사하였으며 유기용매추출물에서 강한 활성을 확인하였다. 3개의 유기용매 중 benzene 추출물이 가장 높은 Raffaelea quercus-mongolicae의 균사성장을 억제하였다.

Keywords

References

  1. Jeon, S. M., K. H. Ka, and K. H. Kim. 2010. Antagonistic properties of mushroom strains to Korean oak wilt pathogen, Raffaelea quercus-mongolicae. Kor J. Mycol. 38: 62-68.
  2. Keiko, K. 2001. Responses of Quercus sapwood to infection with the pathogenic fungus of a new wilt disease vectored by the ambrosia beetle Platypus quercivorus. J. Wood Sci. 47: 425-429.
  3. Kim, S. W., K. S. Kim, K. Lamsal, Y. J. Kim, S. B. Kim, M. Jung, S. J. Sim, H. S. Kim, S. J. Chang, J. K. Kim, and Y. S. Lee. 2009. An in vitro study of the antifungal effect of silver nanoparticles on oak wilt pathogen Raffaelea sp. J. Microbial. Biotechnol. 9: 760-764.
  4. Kinuura, H. 2002. Relative dominance of the model fungus, Raffaelea sp., in the mycangium and proventriculus in relation to adult stages of the oak playpodid beetle, Platypus quercivorus (Coleoptera; Platypodidae). J. For. Res. 7: 7-12.
  5. Lee, S. H., K. H. Kim, S. C. Shin, J. Kim, and Y. S. Yi. 2007. Soil Microorganisms against Cryphonectria parasitica. J. Appl. Biol. Chem. 50: 173-174.
  6. Seo, S. T., K. H. Kim, S. H. Lee, Y. N. Kwon, C. H. Shin, H. J. Kim, and S. Y. Lee. 2010. Genotypic characterization of oak wilt pathogen Raffaelea quercus-mongolicae and R. quercivora strains. Res. Plant Dis. 16: 219-223
  7. Watve, M. G., R. Tickoo, M. M. Jog, and B. D. Bhole. 2001). "How many antibiotics are produced by the genus Streptomyces?". Arch. Microbiol. 176: 386-390.
  8. Williams, S. T., M. Goodfellow, and G. Alderson. 1989. Genus Streptomyces pp. 2452-2492. In Bergey's Manual of Systematic Bacteriology. vol. 4.
  9. Williams, S.T. and F. L. Davis. 1967. Use of a scanning electron microscope for the examination of actinomycetes. Journal of General Microbiology. 48: 171-177.

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  1. Screening of Antifungal Microorganisms with Strong Biological Activity against Oak Wilt Fungus, Raffaelea quercus-mongolicae vol.34, pp.5, 2012, https://doi.org/10.7747/jfes.2018.34.5.395
  2. Screening of Endophytic Fungal Isolates Against Raffaelea quercus-mongolicae Causing Oak Wilt Disease in Korea vol.48, pp.6, 2012, https://doi.org/10.1080/12298093.2020.1830486