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http://dx.doi.org/10.4014/kjmb.1110.10003

Antifungal Property of Microorganisms against Korea Oak Wilt Pathogen, Raffaelea quercus-mongolicae  

Lee, Sang-Hyun (Division of Forest Insect Pests and Disease, KFRI)
Lee, Seung-Kyu (Division of Forest Insect Pests and Disease, KFRI)
Kim, Jae-Young (Department of Biochemistrye, Hoseo University)
Lee, Chong-Kyu (Department of Forest Resources, Jinju National University)
Kim, Kyung-Hee (Division of Forest Insect Pests and Disease, KFRI)
Yi, Yong-Sub (Department of Biochemistrye, Hoseo University)
Publication Information
Microbiology and Biotechnology Letters / v.40, no.1, 2012 , pp. 66-69 More about this Journal
Abstract
Five strains out of 200 candidate strains (SG 1-9, 1-12, SG 2-8, 2-10, and 2-17) were selected to determine their antifungal activity against Raffaelea quercus-mongolicae. The 16S rDNA sequences of the five strains were determined by sequencing analysis and analyzed by the homology of the blast program at NCBI. The homology search showed that SG 1-9 and 1-12 had a 98% homology with Streptomyces cinnamoneus and 98% homology with Burkholderia cepacia, while SG 2-8, 2-10, and 2-17 had a 99% homology with Streptomyces fradiae, a 97% homology with Staphylococcus epidermidis, and a 99% homology with Staphylococcus epidermidis. Out of the five selected strains, organic extract and protein extracts of SG2-17 strain broth were employed to determine antifungal activity against Raffaelea quercus-mongolicae. The organic extract exhibited antifungal activity, but the protein extracts did not demonstrate such an activity. Three organic solvents, butanol, benzene, and ethyl acetate, were also used for determination of antifungal activities. The activity measurements revealed that benzene extract possessed the greatest inhibitory effect on the growth of Raffaelea quercus-mongolicae, with the next highest being butanol extract, and ethyl acetate extract being the lowest.
Keywords
An antifungal activity; organic extract; Raffaelea quercus-mongolicae; 16S rDNA sequences;
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Times Cited By KSCI : 2  (Citation Analysis)
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