• Mycobiology
• /
• v.31 no.4
• /
• pp.200-204
• /
• 2003

Cultural characteristics and phylogenic relationships were investigated and classified among collected strains in Pholiota spp. which contain P. adiposa, P. squarrosa, P. nameko etc. They were tested on the four different media(PDA, MCM, YM, MEA) and sawdust(Alder, Oak, Pine, Popular) substrates. There was a little variation according to the media and sawdust substrates, although PDA and popular sawdust substrate seemed to be better. Most strains showed white colonies, but some strains were brown. Mycelial growth length differed according to the strains. To classify species, the internal transcribed spacer regions(ITS) of the ribosomal DNA(rDNA) repeats from Pholiota spp. were amplified using polymerase chain reaction(PCR) and then sequenced. According to the analysis of ITS sequences, they were classified into five clusters. Their spacer regions were $644{\sim}700$ nucleotides in length. The reciprocal homologies of each ITS region among these strains were ranged from $49.6{\sim}99.9%$. The phylogenic analysis might give a criterion to classify species in the collected strains.

• Mycobiology
• /
• v.40 no.3
• /
• pp.164-167
• /
• 2012

The effects of aeration through lid filters on the hyphal growth of Lentinula edodes (oak mushroom) in sawdust cultivation bags were investigated. The aeration treatment levels were traditional 27 mm hole cotton plugs, cotton balls and combinations of seven hole sizes ${\times}$ two hole positions (up and under) in the lids covering plastic bags containing 1.4 kg sawdust medium at 63% moisture that had been autoclaved for one hour and inoculated with sawdust spawn of L. edodes strain 921. Aeration treatment effects were measured based on the $CO_2$ concentration at the 15th wk, as well as the hyphal growth rate and degree of weight loss of bags every 14 days for 15 wk. In bags with traditional cotton plugs, the $CO_2$ concentration was $3.8{\pm}1.3%$, daily mean hyphal growth was $2.3{\pm}0.6mm$ and daily mean weight loss was $0.84{\pm}0.26g$. In the bags with 15 mm diameter holes, the $CO_2$ concentration was $6.0{\pm}1.6%$, daily hyphal growth was $2.8{\pm}0.2mm$ and daily weight loss was $0.86{\pm}0.4g$. The bags with 15 mm holes had a higher $CO_2$ concentration and lower water loss than bags with other hole sizes, but the hyphal growth was not significantly different from that of other bags. The weight loss of bags increased proportionally relative to the lid hole sizes. Taken together, these results indicate that traditional cotton plugs are economically efficient, but 15 mm hole lids are the most efficient at maintaining hyphal growth and controlling water loss while allowing $CO_2$ emissions.

• Journal of Mushroom
• /
• v.16 no.4
• /
• pp.331-333
• /
• 2018

The incorporation of Shiitake culture into sawdust is a widely utilized technique that can assist in reducing the cost and time consumption associated with oak cultivation. In sawdust cultivation, browning of the surface mycelia is an important stage with respect to the utility and longevity of the sawdust media. Surface browning forms a protective coating on the substrate, which can inhibit the invasion of pathogens and suppress water evaporation. Several different light sources (red LED, white LED, blue LED, and fluorescent light) were used and the intensity of illumination was carefully controlled (1.5, 10.5, $20.5{\mu}mol/m^2s$ for LEDs and 10, 100, 300 lux for the fluorescent light) to induce browning. The light sources were regulated via a 1 h on/off cycle in a controlled room environment at a temperature of $20^{\circ}C$, 60% humidity, and 1200 ppm $CO_2$ concentration for 60days. The browning effect varied depending on the source and the intensity of illumination. This effect was most effectively induced at $1.5{\mu}mol/m^2s$ for the red and blue LEDs. All light sources induced less browning at the highest intensity of illumination. This indicates that intensity values higher than $20.5{\mu}mol/m^2s$ in the case of the LEDs and 300 lux for the fluorescent light are not effective. After harvesting of the fruit bodies, we measured the weight, length, and width of the pileus and stipe in addition to their chromaticity and hardness. Treatment with $1.5{\mu}mol/m^2s$ blue LED produced the best harvest with the highest average chromaticity, weight (21.2 g), stipe length (30.8 mm), and hardness (377.9 g), with a fine length and width of the pileus.

• The Korean Journal of Mycology
• /
• v.6 no.2
• /
• pp.31-36
• /
• 1978

The study has been carried out to invetigate the possibility of oak mushroom (shiitake) cultivation on Ban Oak (Quercus incana) growing naturally in Simlaregion, India. The survival and growth of oak mushroom mycelium and fruit body formation on Q. incana as well as the composition of the log were compared with that of Bristle-tooth oak (Q. serrata) which are being used for mushroom cultivation in Korea. The results are summarized as follows. 1. The content of alcohol-benzen extract, NaOH extract, NaOH extract and ash were higher in Q. incana than in Q. Serrata. While cellulose and pentosan were less in the formers. 2. The mycelial growth of oak mushroom were more rapid on sawdust medium of Q. Serrata than on Q. incana. However the mycelial growth on the later were more compact. 3. The mycelial growth of oak mushroom were more rapid on the logs of Q. serrata than Q. incana. The mycelium survived well on both two species, and no difference in the survival rate of mycelium were observed. 4. The first fruit bodies on logs of Q. Serrata and Q. incana were appeared 16 months inoculation of spawn. 5. In view of the above results. it seems that the cultivation of oak mushroom (shiitake) on Ban oak (Q. incana), growing in India, is possible.

• The Korean Journal of Mycology
• /
• v.45 no.2
• /
• pp.121-131
• /
• 2017

We evaluated the antioxidant properties of Lentinula edodes upon sawdust bag cultivation with 5 oak substrates: Quercus acutissima, Q. mongolica, Q. serrata, Q. aliena, and Q. variabilis. We found that the optimal extraction conditions were 70% (v/v) methanol shaken at 150 rpm at $25^{\circ}C$ and 150 rpm for 24 h. The methanolic extracts from L. edodes contained high phenolic and flavonoid contents, they also exhibited stronger antioxidant activities. The total phenolic contents and total flavonoid contents of the mushroom extracts ranged from 2.37 to 3.12 milligrams of gallic acid equivalents per gram of dried mushroom (mg GAE/g) and 0.48 to 0.48 milligrams of quercetin equivalents per gram of dried mushroom (mg QE/g), respectively. In addition, the mushroom extracts exhibited 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (15.8% to 89.4%) at 2 to 10 mg/mL, ferric reducing antioxidant power (0.153 to 0.425) at 5 to 20 mg/mL, and reducing power (0.078 to 0.359) at 5 to 20 mg/mL, respectively. Q. aliena more effectively increased total phenolic contents, total flavonoid contents, and antioxidant activities than the other oak substrates.

• Journal of Mushroom
• /
• v.21 no.3
• /
• pp.185-189
• /
• 2023

This study was conducted to develop a renewable and sustainable bio-material to replace polystyrene (EPS) in fungal-mycelium-based composite using agricultural by-products. Four mushrooms (Ganoderma lucidum, Fomitella fraxinea, Phellinus linteus, and Schizophyllum commune) were cultured in an oak sawdust plus rice bran substrate to select the mushroom with the best growth. The mycelia of G. lucidum showed the best growth. To investigate the optimal mixing ratio with spent mushroom substrate (SM) and oak sawdust (OS), samples were prepared by mixing SM and OS at ratios of 50%:50%, 60%:40%, and 80%:20% (w/w). Each substrate was then inoculated with G. lucidum. G. lucidum showed the best mycelial growth of 140.0 mm in the substrate with SM and OS mixed at a 60%:40% ratio. It was also found that the substrate with SM and OS mixed at a 60%:40% ratio had the best handling properties. The compressive strength of mycelial materials inoculated with G. lucidum was in the range of 300-302 kgf mm^-1, and the materials were four times stronger than polystyrene materials. These results indicate that substrates comprising spent mushroom substrate mixed with oak sawdust can be successfully upcycled to mycelium-based composite materials using G. lucidum. This represents a sustainable approach.

• Journal of the Korean Wood Science and Technology
• /
• v.43 no.6
• /
• pp.757-767
• /
• 2015

This study was conducted to investigate the effects of moisture content and particle size of sawdust on the fuel characteristics of wood pellets produced with Mongolian oak (Quercus mongolica, QUM), red pine (Pinus densiflora, PID) and larch (Larix kaempferi, LAK) sawdust using a flat-die pelletizer. Prior to produce wood pellets, the sawdust was controlled to the moisture content of 8, 11, 12% and was screened to the particle size of 2 and 4 mesh. In the analysis of its chemical composition, QUM had a high ash content, and PID and LAK contained large amount of lignin. In case of the fuel characteristics, PID pellets had the lowest moisture content of pellets (P-MC), and LAR pellets was found to have the highest bilk density (BD) and durability (DU). With the increase of moisture content of sawdust (S-MC), P-MC and DU of QUM, PID and LAK pellets increased, but BD of QUM and LAK pellets decreased. When size of sawdust used for the production of wood pellets decreased, P-MC and BD of LAK pellets and BD of QUM pellets increased. Decrease of particle size contributed to the increase of DU of QUM, PID and LAK pellets. In addition, BD and DU of QUM pellets produced with 12% S-MC sawdust increased as its particle size reduced. For LAK pellets, DU was not influence by particle size in the S-MCs of 10% and 12%, but increased with the decrease of particle size in the S-MC of 8%. Based on the results and economical aspects, 10% MC and 2 mesh paricle size for QUM sawdust and 12% MC and 2 mesh particle size for PID sawdust might be optimal conditions for pellets production, and fuel characteristics of wood pellets produced by the conditions greatly exceeded the minimum requirements for the $1^{st}$-grade wood pellets of the standard designated by Korea Forest Research Institute.

• Journal of the Korean Wood Science and Technology
• /
• v.21 no.1
• /
• pp.7-14
• /
• 1993

To increase the production of mushrooms and the economic efficiency of mushroom cultivation, the pinchips(PC) and sawdusts(SD) of three Korean oak species were tested as cultivating media for Lentinus edodes with varing the composition of the media. The results are as follows. 1. Organic acids(tannic acid and citric acid), which were added in the PC medium to enrich the quality of mushrooms, did not increase the growths of the mycelia and the quality. 2. The mushrooms were able to be harvrested from six months after beginning the cultivation. No significant differences were found among the qualities of the mushrooms produced by various treatments. 3. The production rate of mushroom was relatively high with, 28.4~29.3% (700~730gr./2,500gr pack). The net profit estimated was about 1,450 won per pack.

• Proceedings of the Korean Society for Bio-Environment Control Conference
• /
• 2002.04a
• /
• pp.97-100
• /
• 2002

표고버섯은 약리 효과 면에서 Lentinan에 의한 항암 효과와 Eritadenine의 콜레스테롤 수치를 낮추는 효과 등이 있기 때문에(이, 2000), 세계적으로 표고의 생산과 소비량은 급격하게 증가하고 있다(Royse, 2002). 국내 대부분의 표고 생산은 많은 원목과 노동력이 요구되지만 어느 정도의 품질이 보장되는 원목에 의존하고 있기 때문에, 톱밥재배가 정착되기 위해서는 품질을 증진시키는 것이 필요하다. (중략)

• Journal of Mushroom
• /
• v.7 no.3
• /
• pp.115-121
• /
• 2009

Recently sawdust cultivation of Shiitake mushroom (Lentinula edodes ) is getting increased because log cultivation is getting difficult to get oak logs. It is important to make mycelia browning on the substrate surface in sawdust cultivation. This browned surface plays an important role like as artificial bark of the oak log, which protects the other pests and suppresses water evaporation in the substrate. The period for mycelia browning is so long that the sawdust cultivation of Shiitake mushroom can not spread well into the mushroom farms. In this article we would like to discuss about the effect of environmental condition to the mycelial browning during sawdust bag cultivation for the To reduce the period required for browning of substrates, sawdust substrates was illuminated light with difference intensity. One hundred Lux light illumination was needed for producing normal yield of fruit body but fruit body yield was low and abnormally shaped fruit body was produced when cultured under the dark condition of incubation. Illumination over 200lux is necessary for the successful browning of substrates during incubation. Optimum incubation temperature for browning of substrates and fruiting was $25^{\circ}C$. The treatment of cotton plug with different size to identify the effect of aeration on the browning of substrates and fruiting showed rapid mycelial growth and reduced the periods for browning as the size of cotton plug was bigger. However, yield of fruit body was the highest at 16mm diameter cotton plug as compared to 20mm of that. $CO_2$ content in vessel of substrates was low as the size of cotton plug was bigger during incubation. $CO_2$ content during incubation of substrate was highest in periods between 8 week and 14 week after inoculation of shiitake when substrate was changed color into brown. $C_2H_4$ content in vessel with substrates was highest at 8mm diameter cotton plug and it was increased by order of 12, 16, 20, 0, 4 mm diameter cotton plug during substrate incubation. Sawdust substrate was soaked in cold water for different time to identify soaking effect of sawdust substrate on fruit body yield and activities of enzymes in these substrates were investigated. The fruit body yield was increased up to 40% by soaking substrates in comparison with unsoaked substrates. The soaked substrates showed 165, 175g/1,000ml at treatment of 4 and 15 hours, respectively. Cellulose activities in soaked substrates were not changed with soaking time, but activities of laccase, lignin degradation enzyme, were drastically increased up to 4 times in comparison with unsoaked substrates.