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http://dx.doi.org/10.4489/KJM.20170015

Antioxidant Properties of Lentinula edodes after Sawdust Bag Cultivation with Different Oak Substrates  

Seo, Sooyoung (Wood Chemistry and Microbiology Division, National Institute of Forest Science)
Park, Youngae (Wood Chemistry and Microbiology Division, National Institute of Forest Science)
Jang, Yeongseon (Wood Chemistry and Microbiology Division, National Institute of Forest Science)
Ka, Kang-Hyeon (Wood Chemistry and Microbiology Division, National Institute of Forest Science)
Publication Information
The Korean Journal of Mycology / v.45, no.2, 2017 , pp. 121-131 More about this Journal
Abstract
We evaluated the antioxidant properties of Lentinula edodes upon sawdust bag cultivation with 5 oak substrates: Quercus acutissima, Q. mongolica, Q. serrata, Q. aliena, and Q. variabilis. We found that the optimal extraction conditions were 70% (v/v) methanol shaken at 150 rpm at $25^{\circ}C$ and 150 rpm for 24 h. The methanolic extracts from L. edodes contained high phenolic and flavonoid contents, they also exhibited stronger antioxidant activities. The total phenolic contents and total flavonoid contents of the mushroom extracts ranged from 2.37 to 3.12 milligrams of gallic acid equivalents per gram of dried mushroom (mg GAE/g) and 0.48 to 0.48 milligrams of quercetin equivalents per gram of dried mushroom (mg QE/g), respectively. In addition, the mushroom extracts exhibited 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (15.8% to 89.4%) at 2 to 10 mg/mL, ferric reducing antioxidant power (0.153 to 0.425) at 5 to 20 mg/mL, and reducing power (0.078 to 0.359) at 5 to 20 mg/mL, respectively. Q. aliena more effectively increased total phenolic contents, total flavonoid contents, and antioxidant activities than the other oak substrates.
Keywords
Antioxidant activity; Lentinula edodes; Sawdust bag cultivation;
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