• Toxicological Research
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• 제18권2호
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• pp.205-213
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• 2002

This study was undertaken to develop a subacute murine model for predicting occurrence of systemic anaphylaxis and to evaluate efficacy of various immunological parameters as the monitoring indices for the occurrence of anaphyalxis. The murine anaphyalxis model was developed through intraperitoneally sensitizing 100 $\mu\textrm{g}$ ovalbumin (OVA) in the presence of 1 mg alum and 300 ng cholera toxin twice a week interval followed by challenging 500 $\mu\textrm{g}$. OVA intravenously. Typical anaphylaxis symptoms were demonstrated at the both BALB/c mice, a strain prone to type-2 response, and C57BL/6 mice. a strain prone to type-1 response. Level of plasma histamine was approximately 50-fold or 30-fold higher in the mice sensitized with OVA than the mice sensitized with alum plus cholera toxin or the saline-treated mice after OVA challenge, respectively. Sensitization and challenge with OVA significantly enhanced plasma leukotriene $B_4$ level but not IgE levels in comparison with the control mice, which indicated the role of leukotriene $B_4$ for progression of anaphyalxis. Furthermore, among mice suffered from anaphylaxis, levels of OVA-specific IgGl were significantly higher in the BALB/c mice than in the C57BL/6 mice, which implied the genetic susceptibility for the induction of systemic anaphylaxis. Conclusively, simultaneous evaluation of histamine, leukotriene $B_4$, and allergen-specific IgG isotype may serve as more efficient monitoring tool for vaccine-related anaphyalxis.

• Natural Product Sciences
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• 제19권4호
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• pp.347-354
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• 2013

Cordyceps militaris, a traditional medicinal mushroom, produces a component compound, cordycepin (3'-deoxyadenosine). Cordycepin has many pharmacological activities including immunological stimulating, anti-cancer, and anti-infection activities. However, the therapeutic mechanism has not yet been elucidated. In this study, we examined the effects of cordycepin on the antigen-presenting function of antigen-presenting cells (APCs). Dendritic cells (DCs) were cultured in the presence of cordycepin and then allowed to phagocytose microspheres containing ovalbumin (OVA). After washing and fixing, the efficacy of OVA peptide presentation by DCs was evaluated using CD8 and CD4 T cells. Also, we confirmed the protein levels of proinflammatory cytokines through RT-PCR and Western blot analysis. Cordycepin decreased both MHC class I and class II-restricted presentation of OVA and suppressed the expression of both MHC molecules and the phagocytic activity toward exogenous OVA. The class II-restricted OVA presentation-regulating activity of cordycepin was also confirmed using mice that had been injected with cordycepin followed by soluble OVA. Furthermore, cordycepin suppressed the mRNA and protein levels of iNOS, COX-2, pro-inflammatory cytokines in a concentration-dependent manner. These results provide an understanding of the mechanism of the T cell response-regulating activity of cordycepin through the inhibition of MHC-restricted antigen presentation in relation to its actions on APCs.

• 한방안이비인후피부과학회지
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• 제26권1호
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• pp.35-49
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• 2013

Objectives : We aimed to determine therapeutic effects of probiotics-fermented Magnolia denudata(MD) in the allergic rhinitis model mice. Methods : Polyphenol production, DPPH radical scavenging activity and NO inhibition of fermented MD by different bacterial strains were evaluated to select the one that is most suitable for fermentation. Thirty C57BL/6 mice were divided randomly into 5 groups as follows: normal group, ovalbumin(OVA)-treated plus water fed(CON group), OVA-treated plus unfermented MD fed(UMD group), OVA-treated plus fermented autoclaved MD fed(A-FMD group) and OVA-treated plus fermented unautoclaved MD fed(FMD group). After 9 weeks, we observed changes in the blood cell count, OVA-specific IgE level, nasal rubbing, nasal mucosal tissue and body weight. Results : Extract of MD fermented by Bifidobacterium breve(BB) for 48 hours showed the highest anti-oxidant activity and anti-inflammatory activity out of all the other bacterial strains. The number of eosinophil count in A-FMD, FMD group and platelet count in FMD group showed statistically significant decrease(p<0.05). OVA-specific IgE level decreased in all 3 experimental groups, significantly in UMD and A-FMD group. Nasal symptoms were attenuated in all 3 experimental groups, statistically significant in A-FMD and FMD group (p<0.05). Histologically, infiltration of eosinophils into the nasal mucosa decreased in all 3 experimental groups, especially marked decrease in FMD group. Conclusions : According to the above results, it is considered that probiotics-fermented Magnolia denudata has inhibitory effects on the allergic rhinitis animal models.

• 한국축산식품학회지
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• 제25권4호
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• pp.458-464
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• 2005

This study was conducted to evaluate the effect of gamma irradiation for the reduction of an egg allergy in a cake with the irradiated egg white. A white layer cake was manufactured including 10- or 20-kGy-irradiated egg white. Enzyme-Iinked immunosorbent assays (ELISAs) with immunoglobulin (Ig) E from egg-allergic patient and with rabbit anti-ovalbumin (OVA) IgG were used to identify and quantify native OVA in the samples. Concentrations of native OVA detected by IgE and IgG in the control were $432.88{\mu}g/g$ sanple and $375.46{\mu}g/g$ sample, respectively. However, native OVA in white layer cake prepared with 10- and 20-kGy-irradiated egg white were detected at low concentrations detected by IgE to 14.27 and $8.78{\mu}g/g$ sample, respectively. Whereas, IgG recognized OVA more often in 10- and 20-kGy samples than in control. The result appear to suggest that irradiating egg white might reduce ie allergenicity by the conformational change of OVA. Therefore, gamma irradiation could utilize reduce an egg allergy.

• 동의생리병리학회지
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• 제20권3호
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• pp.581-589
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• 2006

These studies were investigated the effects of Gamicheungpyehwadam-tang (CPHDT) on immune-cell regulation in association with bronchial asthma in OVA-induced mouse model. The administration of 400 mg/kg CPHDT significantly reduced the number of total cells in lung, peripheral lymph node and spleen in OVA-induced bronchial asthma mouse model. The administration of 400 mg/kg CPHDT significantly reduced $CD3^+,{\;}CD19^+$and $CD3^+,{\;}CD69^+$ cell numbers separated from lung, peripheral lymph node and spleen in OVA-induced bronchial asthma mouse model. CPHDT significantly reduced $CD3^+/CCR3^+,{\;}CD4^+,{\;}B220^+/IgE^+$, and $CD3^+/DX5^+$ cell numbers separated from lung, peripheral lymph node and spleen in OVA-induced bronchial asthma mouse model in a dose dependent manner, However, CPHDT significantly reduced $CD8^+$ cell numbers from only lung and spleen. The administration of CPHDT significantly reduced $NK^+$ cell numbers separated from lung of OVA-induced bronchial asthma mouse model in all concentrations, but 200 mg/kg CPHDT reduced $NK^+$ cell numbers separated from peripheral lymph node. These results suggest that CPHDT has anti-asthma and anti-allergy effects. In addition to, CPHDT may be useful treatment of asthma based on the further studies about the individual efficacy search of the components of CPHDT and the adding of variety drugs to CPHDT.

• 한국수정란이식학회지
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• 제6권2호
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• pp.37-46
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• 1991

A field trial was performed to evaluate the effects of hormone treatment on estrus induction, ovulation, embryo transfer and reproductive performance in post-weaning sows. This trial involved 61 mixed breed sows of varying parity on a commercial pig farm. Sows were allocated to one of five trials: control group involved 25 sows that were treated with a single intramuscular injection of 5 ml physiological saline, 6 sows received 1,500 IU PMSG on the day of weanning and 500 IU HCG at the onset of estrus in trial I, 7 sows received 750 IU PMSG on the day of weanning and 500 IU HCG at the onset of estrus in trial II, 5 sows were treated with the same as trial II on day 28 after weanning in trial III. and 18 sows were treated with 10 mg PGF$_2$$\alpha$ plus 2 mg estradiol benzoate on day 31 after weanning in trial IV. Ovarian responses were checked by laparotomy and ova were recovered by oviducal flushing between 40 and l00hrs after mating. Fertilized ova were transferred into the oviduts of recipient sows synchroni- zed. The results obtained were summarized as follows: 1. Percentages of sows detected in standing estrus following treatment were 86~100% among trial groups. The interval from treatment to standing estrus(6l.7$\pm$0.5lhrs) in lOmg PGF$_2$$\alpha$ and 2mg estradial henzoate treated group was significantly earlier than in other trial groups(P<0.05). 2. Average number of ovulations was 11.5~37.8 among trial groups. The ovulation rate in 1,500 IU PMSG and 500 IU RCG treated group (37.8$\pm$ 19.87) was significantly different from other trial groups(P<0.05). 3. Ova were recovered by oviducal flushing between 40~ l00hrs after mating and recovery rates of ova wore 91.4% between 40~59hrs. 4. Fertilized ova were transferred into the oviducts of 8 recipient sows synchronized with 7 to 17 ova per animal. Three of the recipients were pregnant and delivered 25 piglets. 5. Four of the donor sows in those embryo collection was not successful were pregnant following oviducal flushing and delivered 23 piglets. 6. Recurrence of estrus and farrowing performance of experimental sows were observed following the experiment was no difference among trial groups, respectively.

• 한국정보과학회논문지:소프트웨어및응용
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• 제33권10호
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• pp.886-895
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• 2006

지문분류는 사전에 정의된 클래스로 입력된 지문을 분류하여 자동지문인식 시스템에서 비교해야할 지문의 수를 줄여준다. 지지벡터기계(support vector machine; SVM)는 패턴인식 분야에서 널리 사용되고 있을 뿐만 아니라 지문분류에서도 높은 성능을 보이고 있다. SVM은 이진클래스 분류기이기 때문에 다중클래스 문제인 지문분류를 위해서 적절한 분류기 생성과 결합 기법이 필요하며, 본 논문에서는 일대다(one-vs-all; OVA) 방식으로 구성된 SVM을 naive Bayes(NB) 분류기를 이용하여 동적으로 구성하는 분류방법을 제안한다. 지문분류에서 대표적으로 사용되는 특징인 FingerCode와 지문의 구조적 특징인 특이점과 의사융선을 사용하여 OVA SVM과 NB 분류기를 학습하고, 포섭구조의 분류기를 구성하여 효과적인 지문분류를 수행한다. NIST-4 데이타베이스에 제안하는 방법을 적용하여 5클래스 분류에 대해서 90.8%의 높은 분류율을 획득하였으며, OVA 전략의 SVM을 다중클래스 분류문제에 적용할 때 발생하는 동점문제를 효과적으로 처리하였다.

• 디지털융복합연구
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• 제14권6호
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• pp.317-323
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• 2016

본 연구의 목적은 백삼, 오미자, 맥문동, 길경, 감초 등으로 구성된 백삼복합물이 난알부민으로 유도된 천식 마우스에서의 천식개선에 대한 융복합 연구를 통한 효능을 확인하는 것입니다. 난알부민으로 천식을 유발한 마우스에 백삼복합물을 100 mg/kg과 300 mg/kg을 경구 투여한 후 혈청 내 Th2 사이토카인 IL-4, IL-5, IL-13을 루미넥스를 이용하여 측정하였다. 또한, 혈청과 혈액 내 난알부민 특이-IgE 수치와 호산구, BALF 내 총 세포수는 자동혈구계산기 및 현미경을 통해 측정하였다. 그 결과, 혈청 내 IL-4, IL-5, IL-13 및 난알부민 특이-IgE 수치가 대조군에 비해 각각 감소하였다. 또한, 혈액 내 호산구 및 BALF 내 총 세포수 역시 감소를 보였다. 이러한 결과는 백삼복합물이 Th2 사이토카인과 천식 요인에 대해 감소가 나타나 효과적인 항천식 치료 약물로서 사용될 수 있음을 시사하는 바이다.

• 농업과학연구
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• 제1권1호
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• pp.35-39
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• 1974

본시험(本試驗)은 과배란처리가토(過排卵處理家兎)에서 많은 양(量)이 채취(採取)할수 있는 복수(腹水)(peritoneal fluid)가 가토수정난자(家兎水精卵子)의 in. Vitro 배양(培養)의 배양액(培養液)으로서 이용(利用)할수 있는지를 검토(檢討)하기 위하여 실시(實施)하였다. 그 결과(結果)를 요약(要約)하면 다음과 같다. 2) 2세포(細胞), 4세포(細胞), 8세포(細胞), 16세포기(細胞期)의 가토난자(家兎卵子)를 복수(腹水)를 써서 $37^{\circ}C$에서 48시간(時間) 배양(培養)한 결과 분할(分割)된 난자(卵子)의 정도(程度)는 각각(各各) 78.1%, 83.3%, 91.7% 및 93.6%로서 이들의 성적(成績)은 가토혈청(家兎血淸)으로 배양(培養)했을 경우보다도 어느 것이나 좋았다. 2) 2세포(細胞)의 난자(卵子)를 복수(腹水)로 96시간(時間) 배양(培養)했을 때 배양난자(培養卵子)의 88%가 상실기이상(桑實期以上)으로 발달(發達)되고 24%가 배반포(胚盤胞)에 이르고 있으며 가토혈청(家兎血淸)으로 배양(培養)한 성적(成績)과 거의 같은 경향(傾向)을 보였다. 이상(以上)과 같은 점(點)에서 가토난자(家兎卵子)의 배양액(培養液)으로서 가토(家兎)의 복수(腹水)가 유효(有效)하라는 것이 입증(立證) 되었다.

• Tuberculosis and Respiratory Diseases
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• 제84권1호
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• pp.55-66
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• 2021

Background: Particulate matter 10 (PM₁₀; airborne particles <10 ㎛) inhalation has been demonstrated to induce airway and lung diseases. In this study, we investigate the effects of PM₁₀ inhalation on RNA expression in lung tissues using a murine model. Methods: Female BALB/c mice were affected with PM₁₀, ovalbumin (OVA), or both OVA and PM₁₀. PM₁₀ was administered intranasally while OVA was both intraperitoneally injected and intranasally administered. Treatments occurred 4 times over a 2-week period. Two days after the final challenges, mice were sacrificed. Full RNA sequencing using lung homogenates was conducted. Results: While PM₁₀ did not induce cell proliferation in bronchoalveolar fluid or lead to airway hyper-responsiveness, it did cause airway inflammation and lung fibrosis. Levels of interleukin 1β, tumor necrosis factor-α, and transforming growth factor-β in lung homogenates were significantly elevated in the PM₁₀-treated group, compared to the control group. The PM₁₀ group also showed increased RNA expression of Rn45a, Snord22, Atp6v0c-ps2, Snora28, Snord15b, Snora70, and Mmp12. Generally, genes associated with RNA splicing, DNA repair, the inflammatory response, the immune response, cell death, and apoptotic processes were highly expressed in the PM₁₀-treated group. The OVA/PM₁₀ treatment did not produce greater effects than OVA alone. However, the OVA/PM₁₀-treated group did show increased RNA expression of Clca1, Snord22, Retnla, Prg2, Tff2, Atp6v0c-ps2, and Fcgbp when compared to the control groups. These genes are associated with RNA splicing, DNA repair, the inflammatory response, and the immune response. Conclusion: Inhalation of PM₁₀ extensively altered RNA expression while also inducing cellular inflammation, fibrosis, and increased inflammatory cytokines in this murine mouse model.