• YAKHAK HOEJI
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• v.48 no.2
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• pp.117-121
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• 2004

Ranunculin, a potent cytotoxic component of P. koreana, was synthesized by reacting (s)-(-)-5-(hydroxymethyl)-2(5H)-furanone with 2,3,4,6-tetra-O-acetyl-$\alpha$-D-glucopyranosyl bromide and successive removal of the acetyl protecting group by 0.5 M HCl/MeOH. A new deacetylation process of the intermediate tetraacetylranunculin was deviced giving a yield of 83% of ranunculin. Protoanemonin, the cytotoxic structural moiety of ranunculin, was synthesized by dehydration of (s)-(-)-5-hydroxymethyl-2(5H)-furanone. Ranunculin showed a moderate cytototoxic activity against A-549 (ED$_{50}$=7.53 $\mu\textrm{g}$/$m\ell$), NIH3T (ED$_{50}$=13.6$\mu\textrm{g}$/$m\ell$), and SK-OV-3 (ED$_{50}$=17.5 $\mu\textrm{g}$/$m\ell$). Meanwhile, protoanemonin also exhibited moderate cytotoxicity against A-549 (ED$_{50}$=9.38 $\mu\textrm{g}$/$m\ell$), NIH3T (ED$_{50}$=13.8 $\mu\textrm{g}$/$m\ell$), and SK-OV-3 (ED$_{50}$=15.1 $\mu\textrm{g}$/$m\ell$). It was found that both of the synthetic products showed a potenter cytotoxicity against A-549.ainst A-549.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.8
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• pp.4131-4136
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• 2016

Background: The population in northeast Thailand continues to present with hepatobiliary abnormalities, particularly periductal fibrosis (PDF) which is the result of chronic infection with liver fluke (Opisthorchis viverini; OV) and may lead to the development of cholangiocarcinoma (CCA). Although the prevalence of OV infection has been decreased due to a liver fluke control program over decades, the prevalence of PDF remains high. This study aimed to investigate demographic factors associated with PDF risk based on ultrasonography (US) screening. Materials and Methods: This cross-sectional study is part of the Cholangiocarcinoma Screening and Care Program (CASCAP), a prospective cohort study. Multiple logistic regression was used for data analysis. Results: In 55,246 subjects, the overall prevalence of PDF was 33.0% (95%CI: 32.6 - 33.4). Males (33.9 %) were at higher risk for developing PDF than females (32.2 %) (ORcrude = 0.93; 95%CI: 0.89 - 0.96; p-value < 0.001). Factors associated with an increased PDF risk, in addition to OV infection, included old age (${\geq}70$ years) (ORadj = 1.28, 95% CI: 1.14 - 1.44, p < 0.001) and hepatitis B infection (ORadj = 1.31, 95% CI: 1.11 - 1.55, p = 0.001). In contrast, number of praziquantel treatments (> 2 times) (ORadj = 0.54, 95% CI: 0.47 - 0.63, p < 0.001) and diabetes mellitus (ORadj = 0.57, 95% CI: 0.49 - 0.65, p < 0.001) were significantly associated with a decreased PDF risk. Conclusions: Future US screening should closely examine older people and hepatitis B subjects for the purpose of PDF surveillance among high risk groups for CCA. However, the results of inverse associations require further investigation in order to confirm our findings.

• Journal of Embryo Transfer
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• v.18 no.3
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• pp.187-193
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• 2003

Recently a protocol was developed that precisely synchronizes the time of ovulation in Hanwoo. Cows were treated with GnRH on Day 0, PGF$_2$$\alpha$ 7 d later, GnRH 2 d later, and then time-inseminated approximately 24 h after this second treatment with GnRH. Ovarian morphology was monitored cows by trans-rectal ultrasonography 6.5MHz linear transrectal probe(Sonovet - 600., Medison co. Korea) from 24 hr to 31 hrs after second GnRH injection. The result obtained summarized as follows - 1. Induced ovulation were 24 to 31hr after the second GnRH injection, but high induced ovulation was 28hr. 2. Conception rate with HML(High meat lin) and HIL(High milk lin) treatment were 48.1％(38/79) and 43.9％(40/91), respectively. 3. Conception rate of 1∼2 parity and 3∼4 parity was 44.3％ and 55％, respectively. 4. Conception rate of spring, autumn was more increased, 47.3％ than summer.

• YAKHAK HOEJI
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• v.43 no.2
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• pp.169-172
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• 1999

The acivity-guided fractionation on the MeOH extract of Bombycis corpus inoculated by Beauberia bassiana 101A led to the isolation of two steroids, 24-ethycholest-4-ene-3,6-dione (1) ergosterol peroxide (2), as active principles. Compounds 1 and 2 exhibited cytotoxicity against cultured human tumor cell lines, A-549, SK-OV-3, SK-MEL-2, XF-498 and HCT-15 with ED50 values ranging from 3.42 to $11.37{\;}\mu\textrm{g}/m$.

• Archives of Pharmacal Research
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• v.24 no.5
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• pp.385-389
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• 2001

Twelve 4-substituted cyclopenta[c]quinoline derivatives were synthesized and evaluated in vitro cytotoxicity against four human cancer cell lines (HOP62, SK-OV-3, MD-MB-468 and T-47D). The compounds 6c and 6e bearing p-anisidine and pyrrolidine side chain were more active than the others.

• YAKHAK HOEJI
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• v.26 no.2
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• pp.117-121
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• 1982

This investigation was intended to develope the quantitative gas liquid chromatographic method for quercetin. The method involves derivatization of the sample with trimethylsilylimidazole containing cholesterol as an internal standard and injection into a gas chromatograph with 3% OV-17 column. The calibration curve for the quercetin was linear over a sample in the range of 4-16.mu.g. The minimum detectable amount using FID in $1.6{\times}10^{-10}$ a.f.s. was determined to be $6.64{\times}10^{-10}mol$.

• Archives of Pharmacal Research
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• v.28 no.9
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• pp.1053-1056
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• 2005

Cytotoxic activitiy of seven hederagenin saponins isolated from the root of Dipsacus asper were investigated in vitro against L1210, HL-60 and SK-OV-3 tumor cell lines by the MTT method. $3-O-\alpha-L-rhamnopyranosyl-(1{\rightarrow}2)-\alpha-L -arabinopyranosyl$ hederagenin (2),\;$3-O-\beta-D­xylopyranosyl-( 1{\rightarrow}3)-\alpha-L-Rhamnopyranosyl-(1{\rightarrow}2)-\alpha-L -arabinopyranosyl$ hederagenin (6) and $3-O-\beta-D-glucopyranosyl-(1{\rightarrow}3)-\alpha-L-rhamnopyranosyl-( 1{\rightarrow}2)-\alpha-L-arabinopyranosyl$ hederagenin (7) exhibited the potent cytotoxicity against the three tumor cell lines with $IC_{50}$ values ranging from 4.7 to 8.7 ${\mu}g/mL$, with the exception of compound 7, which exhibited weak cytotoxic activity against SK-OV-3 $(IC_{50}\;22.5\;{\mu}g/mL)$. Other compounds did not exhibit any cytotoxic activity $(IC_{50}>30{\mu}g/mL)$.

• Herbal Formula Science
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• v.7 no.1
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• pp.107-120
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• 1999

The purpose of this Study was to investigate effects of KaegiBokryengHwan(KBH) on anti-tumor, immunocytes and nitric oxide(NO). This Study estimated the proliferation of L1210 cell lines, HeLa cell lines, SK-OV3 cell lines, MCF-7 cell lines, balb/c mouse 3T3 cell lines, mouse thymocytes and mouse splenocytes and NO production from peritoneal macrophages in vitro. and estimated the proliferation of L1210 cells, mouse thymocytes and splenocytes and NO production from peritoneal macrophages and body weight in L1210 cells-transplanted mice in vivo. The result were obtained as follow ; 1. KBH inhibited significantly SK-OV3 cell lines in vitro. 2. KBH was accelerate significantly the proliferation of balb/c mouse thymocytes in vitro. 3. KBH increased significantly NO production from peritoneal macrophages in vitro. 4. KBH didn't effect the cytotoxicity of L1210 cells in L1210 cells-transplanted mice. 5. KBH was accelerate the proliferation of splenocytes in L1210 cells-transplanted mice. 6. KBH increased NO production from peritoneal macrophages in L1210 cells-transplanted mice. 7. KBH increased the body weight as comparing with control group in L1210 cells-transplanted mice.

• Journal of Embryo Transfer
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• v.12 no.1
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• pp.103-110
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• 1997

The present study was carried out to investigate effects of gonadotropin, age of donor, day of estrus cycle gonadotropin injection started and season on embryo production after superovulation in dairy cattles. Embryo collection records were obtained from 177 embryo donor collections from 98 Holstein cows aged from 3 to 9 years during 4 years(1993~1996) at National Livestock Re-search Institute. Superovulation was induced by injections of 3 gonadotropins(FSH-P, FOLLTROPHIN-v* or SUPER-OV*) beginning on days 9 to 14 of the estrus cycle. Em-bryos were collected from donors using a nonsurgical technique on days 6 to 8 after insemi-nation. The results were as follows ;Number of total and freezable embryos per donor cow was affected by gonadotropin(P<0.01). The more number of total and freezable embryos wereobtained by use of FOLLTROPHIN-V (13.2, 7.4) or FSH-P (11.0, 5.7) than SUPER-OV* (5.0, 2.4). Age of donor, the day that gonadotropin was started or season didn't affect total or freezable embryos(P >0.05).

• Journal of Embryo Transfer
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• v.17 no.1
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• pp.23-32
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• 2002

To establish an effective in vivo Hanwoo embryo production system, several factors including types of FSH analogue, FSH treatment day, grade of Corpus Luteum were examined. Among FSH analogues. Super-OV was shown to have lower effect on transferable embryo production (P<0.01). Regarding the day of FSH treatment, there were significant difference in estrus status of donors(P<0.1), fertilization rate, and transferable and freezable embryo production(P<0.05). Finally, embryo production was significantly(P<0.01) higher in grade A (8.06 ∼9.49) Corpus Luteum which showed more than 6 on the left or right ovary than in grade B, C and D(2.93∼6.28).