• Title/Summary/Keyword: OSM

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Paleomagnetism of Three Seamounts Northwest of the Marshall Islands from Magnetic Inversion (자기이상 역산에 의한 마샬제도 북서쪽 세 해저산의 고지자기 해석)

  • Lee, Tae-Gook;Moon, Jai-Woon;Ko, Young-Tak;Jung, Mee-Sook;Kim, Hyun-Sub;Lee, Kie-Hwa
    • Ocean and Polar Research
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    • v.26 no.4
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    • pp.559-565
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    • 2004
  • Total magnetic field measurements were performed to study paleomagnetism of three seamounts (OSM7, OSM8-1, and OSM8-2) to the northwest of the Marshall Islands in the western Pacific. The study area is located at the Ogasawara Fracture Zone which is a boundary between the Pigafetta and East Mariana basins. The magnetic parameters and paleopoles of three seamounts were derived from inversion of the measured magnetic field. The goodness-of-fit ratio of OSM7 is too low to be included to the estimation of parameters. The complex magnetic anomalies of center, scarcity of flank rift zones and steep slope at OSM7 suggest that the multiple intrusions of magma converge into the center of volcanic edifice. Inclination calculated from the magnetic anomalies of OSM8-1 and OSM8-2 is $-41.2^{\circ}$, and the paleolatitude calculated from the inclination is $23.6^{\circ}S$. The corresponding paleopoles for OSM8-1 and OSM8-2 are $(24^{\circ}42'W,\;48^{\circ}54'N)\;and\;(18^{\circ}18'W,\;48^{\circ}30'N)$, respectively. In comparison with the apparent polar wander path (APWP) of the Pacific plate, the paleopoles are close to 129-Ma pole. The paleopoles and paleolatitudes of OSM8-1 and OSM8-2 suggest that they were formed at similar time and location. The seamounts have drifted northward about $41^{\circ}$ from the paleolatitude to present latitude of seamounts.

Effect of Oncostatin M on Proliferation and Matrix Synthesis of Dermal Fibroblasts (Oncostatin M이 피부섬유모세포의 증식과 기질생성에 미치는 영향)

  • Chun, Kyung Wook;Lim, Hyung Woo;Han, Seung-Kyu;Kim, Woo Kyung
    • Archives of Plastic Surgery
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    • v.35 no.2
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    • pp.115-120
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    • 2008
  • Purpose: Oncostatin M(OSM) is a multifunctional cytokine that belongs to the interleukin(IL)-6 family. Although there have been a number of studies that focused on the role and mechanism of OSM in various organs and tissues, there are few reports on its effect on wound healing. The final purpose of this project is to evaluate the effect of OSM on wound healing. This pilot study was designed to investigate the effect of OSM on proliferation and matrix synthesis of human dermal fibroblasts, which are the major components of the wound healing. Methods: Excess skin that was obtained from patients who underwent skin grafts, was used for this study. From this material, fibroblasts were isolated and cultured. The cultured fibroblasts were treated with one of four concentrations of OSM. The OSM concentrations used were 0, 50, 100, and 200 ng/ml, respectively. After the OSM treatment, cell proliferation was determined by the MTT assay, collagen synthesis by the C1CP method, GAG levels by the Blyscan Dye method. The parameter levels of each group were compared. Results: OSM treatment increased all the components tested in the study. In particular, cell proliferation, GAG synthesis demonstrated statistically significant increases(p<0.05 in the Mann-Whitney U-test). The highest increase in all the components was obtained at a 100 ng/ml concentration of OSM.Conclusion: The results of the present study indicate that OSM stimulates proliferation and matrix synthesis of human dermal fibroblast and the optimal concentration for wound healing is 100 ng/mL.

Inhibition of Growth and Induction of Differentiation of SMMC-7721 Human Hepatocellular Carcinoma Cells by Oncostatin M

  • Kong, N.;Zhang, X.M.;Wang, H.T.;Mu, X.P.;Han, H.Z.;Yan, W.Q.
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.2
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    • pp.747-752
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    • 2013
  • Oncostatin M (OSM) is a multifunctional cellular regulator acting on a wide variety of cells, which has potential roles in the regulation of gene activation, cell survival, proliferation and differentiation. Previous studies have shown that OSM can induce morphological and/or functional differentiation and maturation of many tumor cells. However, the action of OSM on the induction of differentiation of human hepatocellular carcinoma (HCC) has not been reported. Here, we investigated the effects of different concentrations of OSM on human HCC cell line SMMC-7721 growth, proliferation, cell cycling, apoptosis and differentiation in vitro. Cell growth was determined via MTT assay, proliferation by cell cycle analysis, apoptosis by flow cytometry, morphology by transmission electronic microscopy, and cell function by detection of biochemical markers. Our results demonstrated that OSM strongly inhibited the growth of SMMC-7721 cells in a dose-dependent manner, associated with decreased clonogenicity. Cell cycle analysis revealed a decreased proportion of cells in S phase, with arrest at G0/G1. The apotosis rate was increased after OSM treatment compared to the control. These changes were associated with striking changes in cellular morphology, toward a more mature hepatic phenotype, accompanied by significant reduction of the expression of AFP and specific activity of ${\gamma}$-GT, with remarkable increase in secretion of albumin and ALP activity. Taken together, our findings indicate that OSM could induce the differentiation and reduce cell viability of SMMC-7721 cells, suggesting that differentiation therapy with OSM offers the opportunity for therapeutic intervention in HCC.

Interaction of promyelocytic leukemia/p53 affects signal transducer and activator of transcription-3 activity in response to oncostatin M

  • Lim, Jiwoo;Choi, Ji Ha;Park, Eun-Mi;Choi, Youn-Hee
    • The Korean Journal of Physiology and Pharmacology
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    • v.24 no.3
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    • pp.203-212
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    • 2020
  • Promyelocytic leukemia (PML) gene, through alternative splicing of its C-terminal region, generates several PML isoforms that interact with specific partners and perform distinct functions. The PML protein is a tumor suppressor that plays an important role by interacting with various proteins. Herein, we investigated the effect of the PML isoforms on oncostatin M (OSM)-induced signal transducer and activator of transcription-3 (STAT-3) transcriptional activity. PML influenced OSM-induced STAT-3 activity in a cell type-specific manner, which was dependent on the p53 status of the cells but regardless of PML isoform. Interestingly, overexpression of PML exerted opposite effects on OSM-induced STAT-3 activity in p53 wild-type and mutant cells. Specifically, overexpression of PML in the cell lines bearing wild-type p53 (NIH3T3 and U87-MG cells) decreased OSM-induced STAT-3 transcriptional activity, whereas overexpression of PML increased OSM-induced STAT-3 transcriptional activity in mutant p53-bearing cell lines (HEK293T and U251-MG cells). When wild-type p53 cells were co-transfected with PML-IV and R273H-p53 mutant, OSM-mediated STAT-3 transcriptional activity was significantly enhanced, compared to that of cells which were transfected with PML-IV alone; however, when cells bearing mutant p53 were co-transfected with PML-IV and wild-type p53, OSM-induced STAT-3 transcriptional activity was significantly decreased, compared to that of transfected cells with PML-IV alone. In conclusion, PML acts together with wild-type or mutant p53 and influences OSM-mediated STAT-3 activity in a negative or positive manner, resulting in the aberrant activation of STAT-3 in cancer cells bearing mutant p53 probably might occur through the interaction of mutant p53 with PML.

Effects of Osmolarity and Vitamins on the In Vitro Development of Bovine Embryos in a Chemically Defined, Protein-free Culture Medium (무단백 한정배양액에서 삼투압 및 비타민이 소 수정란의 체외발생에 미치는 영향)

  • 김종홍;이상찬;김병기
    • Korean Journal of Animal Reproduction
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    • v.20 no.1
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    • pp.77-84
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    • 1996
  • The purpose of this study was to evaluate effects of osmolarity and vitamins on the in vitro development of embryos. Bovine embryo that had been matured and fertilized in vitro were cultured in simple, chemically defined, protein free medium (mTLP-PVA). When the osmolarity of the medium supplemented 0.35 mM phos-phate and 19 amino acid was changed by NaCI concentration, significantly(p<0.05) higher pro-portion of 1-cell embryos in the medium of 265 or 290 mOsm developed to the morula (32 ~ 35%) and blastocyst(24 ~ 28%) stage. When embryos were transferred to fresh medium containing 5.56mM glucose at 120hrs post-insemination, the highest proportion of embryos developed to mor-ula(40%) and blastocyst(32%) stages at 290 mOsm(p<0.05), although the value in morulae was not significantly different with that(35%) at 315 mOsm. Vitamins in presence of glutamine and amino acids had no beneficial effects on the development of 1-cell embryos to the blastocyst.

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Role of Plasma Osmolality in AVP and Aldosterone Release in Korean Young Men (혈장 삼투질 농도 변동에 따른 항이뇨 호르몬과 Aldosterone 분비조절 : 한국청년)

  • Lee, Won-Jung;Choo, Young-Eun;Koo, Ja-Hoon
    • The Korean Journal of Physiology
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    • v.21 no.2
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    • pp.297-304
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    • 1987
  • A study was carried out to find out the relationship between arginine vasopressin (AVP) release and plasma osmolality in 15 young men (age: 21.4 yr). After an overnight fasting, wale. (20 ml/kg) was imbibed, and venous blood and urine samples were collected every 30 min for 90 min. then 5% saline was infused (0.06 ml/min/kg) for 120 min. AVP was extracted on Sep-Pak column and measured by radioimmunoassay. Under basal condition, plasma osmolality (pOsm), AVP (pAVP) and aldosterone (pAldo) levels were 286.5 mOsm/kg, 1.1 pg/ml, and 140 pg/ml, respectively. pAVP became undetectable during maximum water diuresis, and increased in response to hypertonic saline infusion. pAVP level began to increase when pOsm was above 280 mOsm/kg. Changes in urinary AVP excretion (uAVP) was parallel to pAVP levels. The fall in pAVP was followed by a decrease in uAVP, uOsm and an increase in free water clearance, while the later rise in pAVP was followed by an increase in uAVP, uOsm and a decrease in free water clearance. When pooling all data together, relationships between pAVP and pOsm, and uAVP and uOsm were best expressed by an exponential relationship (r=0.78, 0.86, respectively). pAldo level decreased to 71 pg/ml after water ingestion, and decreased further to 30 pg/ml 2 hr after 5% saline infusion. Even at the same pNa, pAldo level during dehydration state was significantly higher than during hydration state. Negative exponential relation (r=-0.59) was observed between pAldo and pNa. Response to change in body fluid volume was greater in aldosterone than in AVP release.

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Effects of Organic Selenium Mix on the Performance, Carcass Characteristics, Tissue Selenium Distribution, and Economic Value in Finishing Hanwoo Steers (유기셀레늄 혼합제 급여가 비육말기 거세한우의 성장, 도체성적, 체내 셀레늄 분포 및 경제성에 미치는 영향)

  • Kim, D.K.;Jung, D.U.;Sung, H.G.
    • Journal of Animal Science and Technology
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    • v.47 no.6
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    • pp.975-984
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    • 2005
  • This study fulfilled to investigate the feed efficiency, tissue selenium distribution, carcass characteristic and economic value in finishing Hanwoo steers fed organic selenium mix (OSM) which included seleno-yeast, rumen culture and other microbial supplements. Forty five finishing Hanwoo steers were tested for 4 months dividing to three feeding groups: OSM add as 0.5 ppm Se of DM feeds (0.5 ppm OSM), OSM enriched add as 1.0 ppm Se of DM feeds (1.0 ppm OSM) and basal diet without OSM (control). The total weight gains, the average daily gains and the feed intakes were not differ in treatments (p > 0.05). No differences (p > 0.05) were noted for hot carcass weight, loin eye area, backfat thickness, meat yield index, meat color, fat color, tenderness and maturity. However, the 1.0 ppm OSM showed better performances for feed requirement, TDN per gain, meat yield grade and meat quality grade compared to other groups. Tissue selenium distribution was increased by organic selenium feeding: higher Se concentration in liver and rump of 0.5 ppm OSM (p < 0.05), and kidney, liver, sirloin and rump of 1.0 ppm OSM (p < 0.05) than the tissues of control group. Generally, tissue selenium was the highest value in 1.0 ppm OSM and showed higher concentrate in order; kidney, liver, sirloin and rump. The income over feed cost was 1.06-fold higher in 1.0 ppm OSM than control group. In conclusion, organic selenium mix supplementation and its amounts were not influenced to feed intake, body gain and carcass characteristic but significantly increased tissue selenium. Therefore, these results suggest that finishing Hanwoo steer fed an enriched organic selenium mix with proper probiotics is able to produce “high-Se” beef as high bioavailable form as well as create a beneficial opportunity on Hanwoo farm.

Diagnosis of Multiple Crosstalk-Faults in Optical Cross Connects for Optical Burst Switching (광 버스트 스위칭을 위한 광 교환기에서의 다중 누화고장 진단기법)

  • 김영재;조광현
    • Journal of Institute of Control, Robotics and Systems
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    • v.9 no.3
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    • pp.251-258
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    • 2003
  • Optical Switching Matrix (OSM) or Optical Multistage Interconnection Networks (OMINs) comprising photonic switches have been studied extensively as important interconnecting blocks for Optical Cross Connects (OXC) based on Optical Burst Switching (OBS). A basic element of photonic switching networks is a 2$\times$2 directional coupler with two inputs and two outputs. This paper is concerned with the diagnosis of multiple crosstalk-faults in OSM. As the network size becomes larger in these days, the conventional diagnosis methods based on tests and simulation become inefficient, or even more impractical. We propose a simple and easily implementable algorithm for detection and isolation of the multiple crosstalk-faults in OSM. Specifically. we develop an algorithm for isolation of the source fault in switching elements whenever the multiple crosstalk-faults arc detected in OSM. The proposed algorithm is illustrated by an example of 16$\times$16 OSM.

Effect of Oncostatin M on Wound Healing Activity of Diabetic Fibroblasts in vitro (Oncostatin M이 당뇨 환자 섬유모세포의 창상치유능에 미치는 영향)

  • Lim, Hyung Woo;Chun, Kyung Wook;Han, Seung-Kyu;Kim, Woo Kyung
    • Archives of Plastic Surgery
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    • v.35 no.4
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    • pp.355-359
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    • 2008
  • Purpose: Oncostatin M(OSM) has been known as a role in fibrosis and anti-inflammatory effects of various organs and tissues. Although there have been a number of studies which are focused on the roles and mechanisms of OSM, there are few reports on its effects in chronic wound healing. The purpose of this study is to evaluate the effects of OSM in wound healing activities of dermal fibroblasts of chronic wound in vitro. In particular, this study is focused on cell proliferation and synthesis of collagen and glycosaminoglycan(GAG), which are the major components of the extracellular matrices, of diabetic fibroblasts. Methods: Fibroblasts were isolated from excess skin that was obtained from diabetic foot ulcer patients who underwent debridement. The isolated fibroblasts were cultivated in presence of OSM(100 ng/mL). Cell proliferation, collagen synthesis and GAG levels were compared. Results: All the components tested in this study increased in OSM treatment group. In particular, collagen and GAG synthesis demonstrated statistically significant increases(p<0.05 in the Mann-Whitney U-test). Conclusion: These results indicate that OSM increases wound healing activities of dermal fibroblasts of chronic wound in vitro.

Influence of Osmolality of Complete Semen Extender on Motion Characteristics of Frozen-thawed Ram Spermatozoa

  • Joshi, Anil;Mathur, A.K.;Naqvi, S.M.K.;Mittal, J.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.12
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    • pp.1716-1721
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    • 2006
  • The present study was conducted to observe the effect of osmolality of glycerolated TEST-yolk glycerol extenders on post-thawing sperm kinematics of ram spermatozoa of the native Malpura breed maintained in a semi-arid tropical environment. Good quality semen obtained from adult rams was pooled, split and diluted to 1,000 million spermatozoa per ml in complete TEST-yolk-glycerol extenders of 900, 1,200, 1,500 and 1,800 mOsm/kg osmolality. Diluted semen samples were loaded in 0.25 ml straws and cooled down to $-125^{\circ}C$ freezing temperature at the rate of $-25^{\circ}C$ per minute under controlled conditions before plunging into liquid nitrogen for storage. The thawing of straws was performed at $50^{\circ}C$ in a water bath for 10 seconds and sperm kinematics of the frozen-thawed spermatozoa were assessed by a computer-assisted sperm analysis technique. Osmolality of diluent had no significant effect on post-thawing % motility, % rapid, % medium and % slow moving frozen-thawed spermatozoa but significantly (p< 0.05) affected the % linearity and % straightness. The post-thawing % motility and % rapid motile spermatozoa were highest in samples extended in diluent of 1,500 mOsm/kg osmolality and lowest in 900 mOsm/kg. The curvilinear velocity of spermatozoa was significantly (p<0.05) higher for samples extended in 1,800 mOsm/kg, compared to those in 900 and 1,200 mOsm/kg, but the effect was not significantly different to those extended in diluent of 1,500 mOsm/kg osmolality. The study indicated that ram spermatozoa could tolerate a wide osmolality range for dilution in the complete TEST-yolk-glycerol extender for their cryosurvival. The highest recovery of motile spermatozoa following thawing was achieved in samples extended in the TEST-yolk-glycerol diluent of 1,500 mOsm/kg osmolality.