Browse > Article

Effect of Oncostatin M on Wound Healing Activity of Diabetic Fibroblasts in vitro  

Lim, Hyung Woo (Department of Plastic Surgery, Korea University College of Medicine)
Chun, Kyung Wook (Department of Plastic Surgery, Korea University College of Medicine)
Han, Seung-Kyu (Department of Plastic Surgery, Korea University College of Medicine)
Kim, Woo Kyung (Department of Plastic Surgery, Korea University College of Medicine)
Publication Information
Archives of Plastic Surgery / v.35, no.4, 2008 , pp. 355-359 More about this Journal
Abstract
Purpose: Oncostatin M(OSM) has been known as a role in fibrosis and anti-inflammatory effects of various organs and tissues. Although there have been a number of studies which are focused on the roles and mechanisms of OSM, there are few reports on its effects in chronic wound healing. The purpose of this study is to evaluate the effects of OSM in wound healing activities of dermal fibroblasts of chronic wound in vitro. In particular, this study is focused on cell proliferation and synthesis of collagen and glycosaminoglycan(GAG), which are the major components of the extracellular matrices, of diabetic fibroblasts. Methods: Fibroblasts were isolated from excess skin that was obtained from diabetic foot ulcer patients who underwent debridement. The isolated fibroblasts were cultivated in presence of OSM(100 ng/mL). Cell proliferation, collagen synthesis and GAG levels were compared. Results: All the components tested in this study increased in OSM treatment group. In particular, collagen and GAG synthesis demonstrated statistically significant increases(p<0.05 in the Mann-Whitney U-test). Conclusion: These results indicate that OSM increases wound healing activities of dermal fibroblasts of chronic wound in vitro.
Keywords
Oncostatin M; Diabetic fibroblasts;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Han SK, Choi KJ, Kim WK: Clinical application of fresh fibroblast allografts for the treatment of diabetic foot ulcers: a pilot study. Plast Reconstr Surg 114: 1783, 2003   DOI   ScienceOn
2 Cavallini M: Autologous fibroblasts to treat deep and complicated leg ulcers in diabetic patients. Wound Repair Regen 15: 35, 2007   DOI   ScienceOn
3 Brown TJ, Lioubin MN, Marquardt H: Purification and characterization of cytostatic lymphokines produced by activated human T lymphocytes. Synergistic antiproliferative activity of transforming growth factor beta 1, interferon-gamma, and oncostatin M for human melanoma cells. J Immunol 139: 2977, 1987
4 Mosley B, De Imus C, Friend D, Boiani N, Thoma B, Park LS, Cosman D: Dual oncostatin M (OSM) receptors. Cloning and characterization of an alternative signaling subunit conferring OSM-specific receptor activation. J Biol Chem 271: 32635, 1996   DOI   ScienceOn
5 Goren I, Kampfer H, Muller E, Schiefelbein D, Pfeilschifter J, Frank S: Oncostatin M expression is functionally connected to neutrophils in the early inflammatory phase of skin repair: Implications for normal and diabetes-impaired wounds. J Invest Dermatol 126: 628, 2006   DOI   ScienceOn
6 Broughton G 2nd, Janis JE, Attinger CE: Wound healing: an overview. Plast Reconstr Surg 117(7 Suppl): 1e-S, 2006   DOI   ScienceOn
7 Heinrich PC, Behrmann I, Haan S, Hermanns HM, Müller-Newen G, Schaper F: Principles of interleukin (IL)-6-type cytokine signalling and its regulation. Biochem J 374: 1, 2003   DOI   ScienceOn
8 Scaffidi AK, Mutsaers SE, Moodley YP, McAnulty RJ, Laurent GJ, Thompson PJ, Knight DA: Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts. Br J Pharmacol 136: 793, 2002   DOI   ScienceOn
9 Hibi M, Nakajima K, Hirano T: IL-6 cytokine family and signal transduction: a model of the cytokine system. J Mol Med 74: 1, 1996   DOI
10 Ihn H, Tamaki K: Oncostatin M stimulates the growth of dermal fibroblasts via a mitogen-activated protein kinase-dependent pathway. J Immunol 165: 2149, 2000   DOI
11 Wallace PM, Macmaster JF, Rillema JR, Rouleau KA, Hanson MB, Burstein SA, Shoyab M: In vivo properties of oncostatin M. Ann N Y Acad Sci 762: 42, 1995
12 Hehenberger K, Kratz G, Hansson A, Brismar K: Fibroblasts derived from human chronic diabetic wounds have a decreased proliferation rate, which is recovered by the addition of heparin. J Dermatol Sci 16: 144, 1998   DOI   ScienceOn
13 Richards CD, Shoyab M, Brown TJ, Gauldie J: Selective regulation of metalloproteinase inhibitor (TIMP-1) by oncostatin M in fibroblasts in culture. J Immunol 150: 5596, 1993
14 Wallace PM, Macmaster JF, Rouleau KA, Brown TJ, Loy JK, Donaldson KL, Wahl AF: Regulation of inflammatory responses by oncostatin M. J Immunol 162: 5547, 1999
15 Chun KW, Han SK, Lee BI, Kim WK: Optimal concentration of OSM for wound healing activity of fibroblasts. J Korea Wound Care Soc 2: 77, 2006