• BMB Reports
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• v.39 no.6
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• pp.782-787
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• 2006

A new protein was cloned and identified as the sixth subunit of Choristoneura fumiferana origin recognition complex (CfORC6). The newly identified 43 kDa protein CfORC6 is much bigger than DmORC6 (25.7 kDa) and HsORC6 (28.1 kDa), though it's 23.85% identical to DmORC6 and 23.81% identical to HsORC6. Although the molecular weight of CfORC6 is close to ScORc6 (50 kDa), CfORC6 is only 14.03% identical to ScORC6. By alignment, it was found that the N-terminal of CfORC6 has about 30% identities with other ORC6s, but about 100aa of C-terminal of CfORC6 has no identity with other ORC6s. Like ScORC6, CfORC6 has many potential phosphorylation sites, (S/T)PXK. Like DmORC6, CfORC6 has leucine-rich region in the relevant site. Northern Blot showed that CfORC6 mRNA is about 2,000nt. Southern Blot confirmed that there is one copy of CfORC6 gene in spruce budworm genome. Western blot showed that infection of Cf124T cells with CfMNPV didn't affect the expression levels of CfORC6, at least up to 26 hr post infection.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.04a
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• pp.263-267
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• 2003

물만 주입한 1군과 물-토양을 주입한 2군, 물-ORC를 주입한 3군, 물-토양-ORC를 전부 주입한 4군의 4개군으로 조건을 차별화하여 2$0^{\circ}C$ 항온 OER시험을 실시하였다. 1군은 약4mg/L, 2군은 3mg/L, 3군은 12~13mg/L, 4군은 11~12mg/L의 DO를 나타내었다. ORC를 투여하지 않은 1군과 2군은 2$0^{\circ}C$ 용존산소 포화선인 Bmg/L에 크게 못미치는 것으로 나타났고 ORC를 투여한 3군과 4군은 포화선보다 3~4mg/L 높은 수치를 나타내었다. 토양을 주입한 2군과 4군에서는 토양 미생물의 영향을 받아 DO가 감소하나 ORC를 투입한 4군에서는 그 영향이 미미하게 나타난다. TCE 분해능 시험은 15$^{\circ}C$에서 항온으로 30일간 시험하였다. 초기 농도 5000ppb가 30일 경과 후 약 4000ppb로 약20%의 분해율을 나타내었고, 메탄을 첨가했을 때 더 잘 분해됨을 보여주었다.

• Journal of Veterinary Clinics
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• v.27 no.6
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• pp.698-703
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• 2010

This study was performed to compare the efficacy of the sodium carboxymethylcellulose (SCMC), oxidized regenerated cellulose (ORC), and their combination with vitamin E for the prevention of postoperative adhesions in the dog. Twenty five dogs were randomly divided into five groups ; non-treated group (Control Group), SCMC-treated group (SCMC Group), ORC-treated group (ORC Group), vitamin E and SCMC-treated group (SCMC + E Group), and vitamin E and ORC-treated group (ORC + E group). After laparotomy, 5 abrasions were made to induce intraperitoneal adhesions on the surface of the ileal serosa. The adhesions were occurred in serosa to mesentary (37.6%), serosa to serosa (24%), serosa to omentum (8.8%) and serosa to parietal peritoneum (3.2%). The incidences of adhesions were 92%, 84%, 64%, 56% and 68% in Control, SCMC, ORC, SCMC+E and ORC + E Group, respectively. The adhesion scores in SCMC+E Group were significantly lower than those in the other groups (p < 0.01). In conclusion, this study showed that oral supplements of vitamin E and intraperitoneal administration of 2% SCMC solution were effective on reducing intraperitoneal adhesions in the dog.

• Journal of Advanced Marine Engineering and Technology
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• v.36 no.5
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• pp.595-602
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• 2012

In this study, for the purpose of reduction of $CO_2$ gas emission and to increase recovery of waste heat from ships, the ORC (Organic Rankine Cycle) is investigated and offered for the conversion of temperature heat to electricity from waste heat energy from ships. Simulation was performed with waste heat from the exhaust gasse which is relatively high temperature and cooling sea water which is relatively low temperature from ships. Various fluid is used for simulation of the ORC system with variable temperature and flow condition and efficiency of system and output power is compared. Finally, 2,400kW output power is obtained by system optimization of the preheater and reheater utilizing waste heat form sea water cooling system.

• Journal of the Korean Institute of Gas
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• v.20 no.6
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• pp.65-72
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• 2016

ORC system was designed and constructed for utilizing the heat of the exhaust gas and coolant released from the gas engine which was modified to use natural gas as a fuel. In this paper the components of the ORC system were designed and manufactured based on measured data of the gas engine. The components are composed of two plate heat exchanger, the 5kW-class expander and multi stage centrifugal pump. The thermodynamic performance of the ORC system was analyzed by using the electric heater. Also, the developed ORC system was implemented to modified natural gas engine. Two gas engines were used to supply heat to the ORC system. As a result of test bench, when the heat source temperature is $110^{\circ}C$ expander shaft power, the pressure ratio and cycle efficiency is 5.22kW, 7.41, 9.09%. As a result of field test, when the heat source temperature is $86^{\circ}C$ expander shaft power, the pressure ratio and cycle efficiency is 2kW, 3.75, 6.45%.

• Journal of the Institute of Electronics and Information Engineers
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• v.53 no.3
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• pp.3-10
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• 2016

To establish a network environment where multiple wireless personal area networks(WPAN) are simultaneously operating in the same area, we need to consider the effects of inter-device interference. The extent of interference effect from neighboring frequency channel interferer may be different depending on the modulation types that are used for constructing the wireless network. If a modulation scheme with high spectral side lobes is used, the spatial separation between devices plays a major role in determining the amount of interference from neighboring frequency channel interferer. In this paper, we investigate the effect of inter-device interference in the overlapped raised cosine offset quadrature-quadrature phase shift keying (ORC-$OQ^2PSK$) modulation based WPAN. By comparing the results with those of GFSK modulation based WPAN, we verify the feasibility of ORC-$OQ^2PSK$ based WPAN.

• Journal of the Korean Institute of Gas
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• v.18 no.2
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• pp.41-47
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• 2014

In this work a thermodynamic performance analysis is carried out for a combined cycle consisted of an organic Rankine cycle (ORC) and a LNG cycle. The combined system uses a low grade waste heat in the form of sensible energy and the LNG cold energy is used for power generation as well as for heat sink. The effects of the key parameters of th system such as turbine inlet pressure, condensation temperature and source temperature on the characteristics of system are throughly investigated. The simulation results show that the thermodynamic performance of the combined system can be significantly improved compared to the normal ORC which is not using the LNG cold energy.

• Child Health Nursing Research
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• v.22 no.4
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• pp.379-389
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• 2016

Purpose: A longitudinal study was conducted to explore flora colonization and oral glucose high-risk newborns during the first 7 days after birth. Methods: Oral secretions of hospitalized newborns were obtained for microbial cultures and glucose test at days 1-7 after birth. Results: Among the total 112 newborns, 40% were girls and 73% were premature. Mean gestational age was $34.4{\pm}3.2$ weeks and weight was $2,266{\pm}697.5$ grams. The most common flora included Streptococcus (28.2%), Methicillin-resistant Staphylococcus aureus (MRSA, 10.9%), Staphylococcus (6.0%) and Coagulase-Negative Staphylococcus (CNS, 4.0%). The average oral glucose level was $29.2{\pm}23.0mg/dL{\sim}58.2{\pm}39.5mg/dL$. Newborns with higher oral glucose than serum (crude odds ratio [ORc] =1.75; 95% confidence interval [CI] =1.03-2.97), phototherapy (ORc=3.30; 95% CI=2.29-4.76) and prone position (ORc= 2.04; 95% CI=1.13-3.69) were more likely to be colonized. Having oral tubes (ORc=0.42; 95% CI=0.29-0.59), parental nutrition (ORc=0.21; 95% CI=0.13-0.32) and antibiotics (ORc=0.51; 95% CI=0.36-0.73) had protective effects. For oral glucose statistical significances existed on time effect among newborns with Streptococcus (F=9.78, p=.024), MRSA (F=7.60, p=.037) or CNS (F=11.15, p=.019) and interaction between time and colonization among newborns with all of four flora (F=2.73, p=.029) or colonization with only Staphylococcus (F=2.91, p=.034). Conclusion: High-risk newborns develop flora colonization at an early period of life. Their clinical features were associated with types and time of oral flora colonization. They need close monitoring and multifaceted intervention to improve oral environment and infection control.

• Biomedical Science Letters
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• v.23 no.4
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• pp.372-379
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• 2017

This study was investigated to test whether paternal DNA that was destined for degradation was properly licensed by testing for the presence of mini-chromosome maintenance protein (MCM) 7 and origin recognition complex (ORC) 2 in the paternal pronuclei. ORC2 is one of the first licensing protein to come on and MCM7 is one of the last licensing protein to come on. Zygotes were prepared by injection of control and treated sperm injection (ICSI). To control for DNA breakage, epididymal spermatozoa were treated with DNase I to fragment the DNA, then injected into oocytes. The presence of MCM7 and ORC2 in the pronuclei of mouse zygotes was tested by immunohistochemistry, just before the onset of DNA synthesis, at 5 h after fertilization, and after DNA synthesis began, at 9 h post fertilization. We found that in all cases, both MCM7 and ORC2 were present in both pronuclei at 5 h after sperm injection, just before DNA synthesis began. This indicates that no matter how extensive the DNA damage, recruitment of licensing proteins to the origins of replication was not inhibited. Sperm DNA fragmentation does not prevent licensing of DNA replication origins. Furthermore, the embryo recognizes DNA that is damaged by nucleases. Our data indicate that the one-cell embryo does harbor a mechanism to prevent the replication of severely damaged DNA from spermatozoa, even though the embryos do not undergo classical apoptosis.

• Journal of Navigation and Port Research
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• v.36 no.5
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• pp.349-355
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• 2012

In this study, for the purpose of reduction of $CO_2$ gas emission and to increase recovery of waste heat from ships, the ORC(Organic Rankine Cycle) is investigated and offered for the conversion of temperature heat to electricity from waste heat energy from ships. Simulation is performed with waste heat from the exhaust gasse which is relatively high temperature and cooling sea water which is relatively low temperature from ships. The result shows that 1,000kW power generation is available from exhaust gas and 600kW power generation is available from sea water cooling system. Different fluid is used for simulation of the ORC system with variable temperature and flow condition and efficiency of system and output power is compared.