• Title/Summary/Keyword: OPT

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Efficient Secretory Expression of Recombinant Endoxylanase from Bacillus sp. HY-20 in Saccharomyces cerevisiae (출아효모(Saccharomyces cerevisiae)에서 Bacillus sp. HY-20균주의 재조합 endoxylanase의 효율적 분비 발현)

  • Kim, Min-Ji;Kim, Bo-Hyun;Nam, Soo-Wan;Choi, Eui-Sung;Shin, Dong-Ha;Cho, Han-Young;Son, Kwang-Hee;Park, Ho-Yong;Kim, Yeon-Hee
    • Journal of Life Science
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    • v.23 no.7
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    • pp.863-868
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    • 2013
  • The XylP gene, which encodes endoxylanase in Bacillus sp. HY-20, was subcloned, and two expression plasmids, pG-xylP and pGMF-xylP were constructed. These plasmids, which contain different signal sequences, XylP s.s and $MF{\alpha}_{opt}$ s.s, respectively, for the secretory expression of endoxylanase, were transformed into Saccharomyces cerevisiae SEY2102 and FY833, respectively. The recombinant endoxylanases were successfully expressed, with a total activity range of 23.7-70.1 unit/ml according to the expression system and host strain. The endoxylanase activity in SEY2102/pGMF-xylP reached a maximum of 88.1 unit/ml in baffled flask culture. Most of the recombinant endoxylanase was efficiently secreted in the extracellular fraction, and the $MF{\alpha}_{opt}$ s.s was more efficient for secreting endoxylanase in yeast than the XylP s.s. Therefore, the expression system developed in this study produces large extracellular amounts of endoxylanase using S. cerevisiae as the host strain, and it could be used in bioethanol production and industrial applications.

Organizational Personality Type and Citizenship Behaviors Perceived by Public Health Center Workers in Chonnam Province, Korea (전남지역 보건소 직원이 인지하는 조직성격유형과 조직시민행동)

  • Cho, Yoo-Hyang
    • Journal of agricultural medicine and community health
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    • v.36 no.1
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    • pp.47-59
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    • 2011
  • Objectives: The purpose of this study was to assess organizational citizenship behaviors (OCBs) and organizational personality types (OPT) perceived by public health center workers and to analyze differences in perceptions according to organizational characteristics. Methods: The subjects of this study were workers at 12 of the 22 public health centers in Chonnam Province, South Korea. To measure organizational characteristics, we employed the OPT Index and OCBs Index. Data were collected for one month, from 15 September to 14 October 2009. Data for 422 workers were used in the final analysis. Descriptive statistics and t?tests were performed as appropriate, utilizing SPSS/WIN 14. Results: Among 16 types of OPT, ESTJ, ISTJ and ESTP were the most prevalent for the 12 public health centers. The average weight of OCBs was 4.77, and courtesy and civic virtue held the first and the second ranks among OCBs, respectively. Statistically significant differences between types of OPT were detected, in that T type was gender (p=.007) while J type was age (p=.010), education (p=.043) and career (p=.000). Statistically significant differences between OCBs were found in age, education and career. Conclusion: Public health centers should have programs to innovate OPT and to improve OCB. The development of such programs should consider characteristics of public health center workers such as age, gender, education and career.

Optimization Algorithm for k-opt Swap of Generalized Assignment Problem (일반화된 배정 문제의 k-opt 교환 최적화 알고리즘)

  • Sang-Un Lee
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.23 no.5
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    • pp.151-158
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    • 2023
  • The researchers entirely focused on meta-heuristic method for generalized assignment problem(GAP) that is known as NP-hard problem because of the optimal solution within polynomial time algorithm is unknown yet. On the other hand, this paper proposes a heuristic greedy algorithm with rules for finding solutions. Firstly, this paper reduces the weight matrix of original data to wij ≤ bi/l in order to n jobs(items) pack m machines(bins) with l = n/m. The maximum profit of each job was assigned to the machine for the reduced data. Secondly, the allocation was adjusted so that the sum of the weights assigned to each machine did not exceed the machine capacity. Finally, the k-opt swap optimization was performed to maximize the profit. The proposed algorithm is applied to 50 benchmarking data, and the best known solution for about 1/3 data is to solve the problem. The remaining 2/3 data showed comparable results to metaheuristic techniques. Therefore, the proposed algorithm shows the possibility that rules for finding solutions in polynomial time exist for GAP. Experiments demonstrate that it can be a P-problem from an NP-hard.

Estimation on Optimum Fishing Effort of Walleye Pollock Fishery in the East Coast of Korea : Based on the Economic Analysis between Danish Seine Fishery and Trawl Fishery for Walleye Pollock (한국 동해 명태 어업의 적정어획노력량 추정 -동해구기선저인망어업과 동해구트롤어업의 경제성분석을 근거로-)

  • 이장욱
    • The Journal of Fisheries Business Administration
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    • v.22 no.2
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    • pp.75-99
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    • 1991
  • A quantitative analysis was carried out to monitor the commercial yield level of walleye pollock Theragra chalcogramma in the east coast of Korea, based on available data on catch and fishing effort, catch per unit of effort including fish prices from 1911 to 1988, using a traditional yield model. The results from the quantitative assessment were based to estimate maximum economic yield (MEY) and optimal fishing effort (E-opt) at MEY. On the other hand, interaction aspects between danish seine fishery and trawl fishery mainly targeting walleye pollock in the east coast of Korea were studied to predict optimal situation in fishing effort level from economic point of view which gives the most benefits to the two fisheries. Total production of walleye pollock in 1911 when its catch record was begun for the first time was about 12, 000 metric tons(M/T), and then the catch trend maintained nearly at the level of 50, 000 M/T per annum, showing a decreasing trend until 1930. The highest production from historical data base on walleye pollock fishery statistics was from the years in 1939 and 1940, about 270, 000 M/T and 26, 000 M/T, respectively. No production of the fish species was recorded during the years from 1943 to 1947, and from 1949 to 1951. From 1952 onwards annual production was only available from the southern part of 38$^{\circ}$N in the east coast. During two decades from 1952 to 1970, the production had sustained about less than 30, 000 M/T every year. Annual production showed an increasing trend from 1971, reaching a maximum level of approximately 162, 000 M/T in 1981. Afterwards, it has deceased sharply year after year and amounted to 180, 000 M/T in 1988. The catch composition of walleye pollock for different fishery segments during 1970~1988 showed that more than 70% of the total catch was from danish seine fishery until 1977 but from 1978 onwards, the catch proportion did not differ from one another, accounting for the nearly same proportion. Catch per unit of effort (CPUE) for both danish seine fishery and trawl fishery maintained a decline tendency after 1977 when the values of CPUE were at level of 800 kg/haul for the former fishery and 1, 300 kg/haul for the latter fishery, respectively. CPUEs of gillnet fishery during 1980~1983 increased to about 3.5 times as high value as in the years, 1970~1979 and during 1987~1988 it decreased again to the level of the years, 1970~1978. The bottom longline fishery's CPUE wa at a very low level (20 kg/basket) through the whole study years, with exception of the value (60 kg/basket) in 1980. Fishing grounds of walleye pollock in the east coast of Korea showed a very limited distribution range. Danish seine fishery concentrated fishing around the coastal areas of Sokcho and Jumunjin during January~February and October~December. Distributions of fishing grounds of trawl fishery were the areas along the coastal regions in the central part of the east coast. Gillnet and bottom longline fisheries fished walleye pollock mainly in the areas of around Sokcho and Jumunjin during January~February and December. Relationship between CPUEs' values from danish seine fishery and trawl fishery was used to standardize fishing effort to apply to surplus production model for estimating maximum sustainable yield (MSY) and optimum fish effort (F-opt) at MSY. The results suggested a MSY of 114, 000 M/T with an estimated F-opt of 173, 000 hauls per year. Based on the estimates of MSY and F-opt, MEY was estimated to be about 94, 000 M/T with a range of 81, 000 to 103, 000 M/T and E-opt 100, 000 hauls per year with a range of 80, 000 to 120, 000 hauls. The estimated values of MEY and E-opt corresponded to 82% of MSY and 58% of F-opt, respectively. An optimal situation in the fishing effort level, which can envisage either simultaneously maximum yield or maximum benefit for both danish seine fishery and trawl fishery, was determined from relationship between revenue and cost of running the fleet : the optimal fishing effort of danish seine fishery was about 52, 000 hauls per year, corresponding to 50 danish seiners and 27, 000 hauls per year which is equal nearly to 36 trawlers, respectively. It was anticipated that the net income from sustainable yield estimated from the respective optimal fishing effort of the two fisheries will be about 3, 800 million won for danish seine fishery and 1, 000 million won for trawl fishery.

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생산현장에서 발생하는 불확실성을 고려한 생산통제기법들의 유용성 분석

  • 이장한;박진우
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 1993.10a
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    • pp.185-190
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    • 1993
  • In this paper, we investigate the effect of production uncertainty - especially demand fluctuation and activity time variation, to production control policies. First, we examine three famous production control policies, namely, MRP, JIT, OPT, from shop floor control perspective and analyze the difference among them. Based on these, simulation studies are performed to draw out the effects of demand fluctuation which are classified into demand lumpiness and demand irregularity, and, the effects of activity time variation which are classified into standard time variation and non-standard time variation. Experimental investigation shows that, in terms of demand fluctuations, MRP is affected by demand lumpiness, but JIT by demand irregularity. And we also see that both MRP and JIT are influenced by standard time variation with respect to activity time variations.

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타부탐색(Tabu Search)의 확장모델을 이용한 '외판원 문제(Traveling Salesman Problem)' 풀기

  • 고일상
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 1996.10a
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    • pp.135-138
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    • 1996
  • In solving the Travel Salesman Problem(TSP), we easily reach local optimal solutions with the existing methods such as TWO-OPT, THREE-OPT, and Lin-Kernighen. Tabu search, as a meta heuristic, is a good mechanism to get an optimal or a near optimal solution escaping from the local optimal. By utilizing AI concepts, tabu search continues to search for improved solutions. In this study, we focus on developing a new neighborhood structure that maintains the feasibility of the tours created by exchange operations in TSP. Intelligent methods are discussed, which keeps feasible tour routes even after exchanging several edges continuously. An extended tabu search model, performing cycle detection and diversification with memory structure, is applied to TSP. The model uses effectively the information gathered during the search process. Finally, the results of tabu search and simulated annealing are compared based on the TSP problems in the prior literatures.

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Implementation of proportional fair scheduler in OFDMA/TDMA wireless access networks (OFDMA/TDMA 시스템에서 PF 스케줄러의 구현)

  • Choi, Jin-Ghoo;Choi, Jin-Hee
    • Journal of The Institute of Information and Telecommunication Facilities Engineering
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    • v.4 no.2
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    • pp.37-43
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    • 2005
  • A simple scheduler satisfying the proportional fairness (PF) was introduced in wireless access networks and revealed that it can achieve a good compromise between total throughput and user fairness. Though it has received much attention for some time, its application was mainly restricted to the single channel systems. In this paper, we study how to implement the PF scheduler in the multi-channel environments such as OFDMA/TDMA. Besides the traditional PF-SC scheme, we propose a new PF-OPT scheme that is the genuine PF scheduler in a sense of maximizing the total log-utility of users. The simulation results show that PF-OPT gives large throughput under the heterogeneous subchannel statistics.

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