• International Journal of Computer Science & Network Security
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• 제23권6호
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• pp.107-114
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• 2023

Successful implementations of DevOps practices significantly improvise software efficiency, collaboration and security. Most of the organizations are adopting DevOps for faster and quality software delivery. DevOps brings development and operation teams together to overcome all kind of communication gaps responsible for software failures. It relies on different sets of alternative tools to automate the tasks of continuous integration, testing, delivery, deployment and monitoring. Although DevOps is followed for being very reliable and responsible environment for quality software delivery yet it lacks many quantifiable aspects to prove it on the top of other traditional and agile development methods. This research evaluates quantitative performance of DevOps and traditional/ agile development methods based on software metrics. This research includes three sample projects or code repositories to quantify the results and for DevOps integrated selective tool chain; current research considers our earlier proposed and implemented DevOps hybrid model of integrated automation tools. For result discussion and validation, tabular and graphical comparisons have also been included to retrieve best performer model. This comparative and evaluative research will be of much advantage to our young researchers/ students to get well versed with automotive environment of DevOps, latest emerging buzzword of development industries.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.108-108
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• 2003

The purpose of this is to investigate the effects of vitrification in open pulled straws (OPS) on in vitro survival of porcine embryos. Blastocysts were produced by in vitro fertilization of slaughterhouse-derived, in vitro matured oocytes with frozen-thawed boar semen, and subsequent culture on granulosa cell monolayer. After frozen-thawing, embryos were culture in NCSU-23 medium with 5 mM hypotaurine, 4 mg/$m\ell$ BSA and 10 ng/$m\ell$ for 48 hrs to survival tests. When blastocysts were frozen-thawed by OPS methods, the embryos with normal morphology were 32.1, 34.5 and 38.9 % in early blastocyst, blastocyst and expanded blastocyat stages. The rates of partial damaged embryos were significantly (P<0.05) higher in early biastocysts than expanded blastocysts. In another experiment, the embryos frozen by OPS methods were cultured for 48 hrs for survival and developmental rates in vitro. The proportions of embryos hatched were 11.8, 20.2 and 33.3% in embryos frozen-thawed at stages of early blastocyst, blastocyst and expanded embryos. On the other hand, The proportions of embryo with normal morphology after culture were 23.5, 25.0 and 33.3% in embryos frozen-thawed at stages of early blastocyst, blastocyst and expanded embryos. These finding indicate the possible broader application for OPS methods that this procedure described is relatively harmless, that it can be used for blastocysts of different developmental stages.

• 한국수정란이식학회지
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• 제19권1호
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• pp.27-34
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• 2004

This study evaluated the efficiency and compared with different materials of loading vessels for vitrification-plastic/glass, copper grid and nylon. The loading method, vitrification, cryop-reservation and warming method of the oocytes were examined. The loading samples prepared in manual or company-made and sterilized, loaded the COCs selected on each samples and cultured for maturation during 40 hours, and then exposed sequentially to ethylene glycol solution. Thawing method was reversely treated and exposed for warmed oocytes. After oocytes were thawed, fertilized and cultured in vitro for 3-4 hours, rates of development and morphological appearance were examined. The results were as summarized: ㆍOPS from company-made or hand-made of the hematocrit micropipettes, NLS from fishing line and EMG from company-made for EM were used for loading oocytes, respectively. ㆍThe efficiency of freezing method and loading convenience were orderly higher in OPS, NLS and EMG. The optimal capacity per vessel was orderly lowered in NLS, EMG and OPS, respectively. ㆍAfter oocytes were warmed, the recovery rate, morphology and rate of development were orderly higher in OPS, NLS and EMG, respectively. ㆍIn conclusion, OPS has the advantages of achieving a little more survival and preserving results than other two loading methods.

• 대한한방내과학회지
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• 제31권3호
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• pp.600-611
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• 2010

Purpose : This study was to observe the nephroprotective effects of the traditional prescription, Opi-san(OPS). OPS has been generally used for treating various edematous renal diseases, including acute renal failure. Methods : Three different dosages of OPS extracts were administered once a day for 28 days (5 mg/kg, single intraperitoneally administered). On the 23rd day after OPS extract treatment, cisplatin was treated. All the rats (6 groups of 8 rats each) were checked as follows. Changes of body weight, kidney weight, serum BUN and creatinine levels were observed, as well as changes to the kidney MDA and GSH contents. The results were compared with captopril 100 mg/kg, of which the effects on cisplatin-induced acute renal failures were already confirmed. Results : Cisplatin-induced ARF were induced by oxidative stress and related lipid peroxidation in the present study. However, these ARF and inhibition of antioxidant effects induced by cisplatin were dose-dependently reduced by treatment of OPS extracts except in the 100 mg/kg treated group. The effects of OPS extracts at 500 mg/kg were similar to those of 100 mg/kg of captopril. Conclusion : This study suggests that OPS extracts 500 mg/kg showed favorable effects on the cisplatin-induced rat's ARF as similar to 100 mg/kg of captopril.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.76-76
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• 2001

The objective of this study was to optimize the vitrification method of in vitro produced bovine embryos. Thus, in vitro produced embryos at 8 cell, morula and blastocyst stages were vitrified on electron microscope grids (EM grids) or in open pulled straws (OPS) with EG5.5 (5.5 M ethylene glycol, 1.0 M sucrose and 10% FBS in m-DPBS medium) freezing solution and their survival rates after thawing were compared. The embryos on EM grids or in OPS were briefly exposed to EG5.5 freezing solution and plunged directly into liquid nitrogen within 30 to 35 sec. Post-thawed embryos were serially diluted in 0.5, 0.25 and 0.125 M sucrose in m-DPBS, each for 1 min, and then cultured in CRI aa medium supplemented with 10% FBS. Embryonic survival rate was assessed as re-expanded and hatched rates of those embryos after warming. The rates of re-expansion embryos did not significantly different between EM grid (8 cell: 42.10%, morula: 66.66% and blastocyst: 77.08%) and OPS (8 cell: 47.36%, morula: 61.90% and blastocyst: 83.33%) methods. In addition, the hatched rates in EM grid (8 cell: 31.57%, morula: 57.14% and blastocyst: 72.91%) were similar to those in OPS (8 cell. 34.21%, morula: 50.00% and Blastocyst: 77.08%). Interestingly, even at the same blastocyst stage, the in vitro survival of day 7 embryos (EM grid: 79.48 and OPS: 87.18%) was higher than those of day 8 embryos (EM grid: 72.10 and OPS: 82.06%). The total cell number of blastocyst developed in vitro after vitrification was examined with Hoechst 33342 staining to compare the embryo quality among different treatment groups. The total cell number of blastocyst was not significantly different between vitrified groups (EM grid: 162.4$\pm$8.0 and OPS: 158.4$\pm$7.1) and unvitrified control (168.0$\pm$5.6). These results indicate that both vitrification containers can provide the high rate of embryo survival. Moreover, the OPS container may not need a cap to protect the container from floating after immersion in L$N_2$. Therefore, this study suggest that bovine embryos can be cryopreserved easily, effectively and successfully by vitrification method using EM grid or OPS with EG5.5 freezing solution. In the future, the Pregnancy rate would be investigated after transfer of our vitrified embryos into the appropriated recipients.

• 한국가축번식학회지
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• 제23권4호
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• pp.313-321
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• 1999

본 연구는 체외생산된 배반포기배의 vitrification 을 위한 용기로서 glass micropipette(GMP)을 이용할 수 있는지, GMP 와 OPS 로 동결융해 후 생존율의 비교 및 GMP vitrification 후 hatching 율의 향상을 위하여 실시하였다. GMP vessel은 열전도율과 수정란을 포함하는 적은 질량 때문에 OPS 보다 동결 및 융해속도를 높일 수 있다. 3개의 체외수정란을 vitrification 용액에 노출시키고 OPS 또는 GMP vessel에 loading 시킨 후 액체질소에 침적하는데까지 20~25초 이내에 실시하였다. 동결ㆍ융해한 배반포기배는 0.25와 15 M sucrose solution 및 TCM 1999에 각각 5분씩 차례로 희석한 후 10% FCS가 첨가된 TCM 199에 24시간동안 배양하였다. OPS(75.9%)와 GMP(80.0%) 방법간의 re-expanding 율은 유의적 (P＜0.05)인 차이가 없었다. OPS(34.1%)와 GMP(37.5%) 방법에서 hatching 율은 intact group(54.3%) 보다도 유의적 (P＜0.05)으로 낮았다. 비록 GMP straw 당 3개 이하의 blastocysts 를 loading 하였더라도 narrow portion(83.3%) 보다도 wide portion(S6.7%)에서 vitrified 되었다면 re-expanding 율이 유의적 (P＜0.05)으로 낮았다. 비록 30초 처리군과 무처리군 간에는 유의적인 차이가 없었지만 0.05% pronase 용액에 30, 60 및 90초간 처리군 (45.9, 54.7 및 57.5%)의 hatching 율은 무처리구 (35.0%) 보다 유의적 (P＜0.05)으로 높았다. 이러한 결과들은 OPS와 GMP vitrification vessel은 체외생산된 배반포기배의 높은 생존율을 얻을 수 있다. 그러나 GMP vessel은 L$N_2$침적 후 vessel의 floating을 방지하기 위한 또 다른 cap 이 필요하지 않다는 유리한 점을 가지고 있다. 수정란의 loading 위치, 즉 narrow 또는 wide portion에 따라 소 체외 생산된 배반포기배의 생존력에 제한적인 요인으로 고려된다. 0.5% pronase 용액에 60 또는 90초간의 노출은 융해후 hatching 율을 향상시킬 수 있었다.

• 한국수정란이식학회지
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• 제23권3호
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• pp.217-222
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• 2008

In previous studies, we reported that sow which was transferred OPS-freezing embryos not able to deliver a piglet (Kim et al, 2004). This study was conducted to investigate a possibility of gilt as recipients which produce piglets after transfer of OPS-freezing embryos. All transferred embryos were prepared by in vitro production (IVP) system. In vitro culture (IVC) medium used glucose-free NCSU23 supplemented with 5mM sodium pyruvate, 0.5 mM sodium lactate and 4 mg/ml bovine serum albumin for 2 days at $39^{\circ}C$. From day 3 of IVC, 10% fetal bovine serum albumin was added to the culture medium. In preparing of freezing embryos, embryos were treated with 7.5 $\mu g/ml$ cytochalasin-B for 30 min and centrifuged at $13,000{\times}g$ for 13 min. And then, embryos were exposed sequentially to an ethylene glycol (EG) solution, aspirated into open pulled straw (OPS), and plunged or thawed into the liquid nitrogen. In embryo transfer (ET), we used two kinds of type (surgical method vs. non-surgical method). In surgical method of embryo transfer, $55\sim65$ embryo were transferred in both uterine horn of two recipient gilts by plastic straw. Non-surgical method which is like artificial insemination was performed on three gilts. Each 140 frozen embryos were transferred to two gilts and 40 fresh embryos to one gilt. Pregnancy establishment was shown one recipient at 45 days after ET. However, the one recipient was also aborted at 58 days after ET. These results suggest that gilts can be considered as a candidate of recipients for OPS-freezing embryo transfer.

• 한국수정란이식학회지
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• 제27권1호
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• pp.63-69
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• 2012

The objective of this study was to investigate the effectiveness of cryopreservation methods for the effect of various vitrification containers, such as EM-grid, OPS, or cryo-loop on the survival and developmental rate of vitrified mouse pronuclear embryos, and mouse cleavage embryo, at 21, 24, 27 and 30 hr after hCG injection. Post-thaw cleavage was similar among treatments, while the developmental rates of mouse blastocyst and hatched blastocyst were higher ($p$ <0.05) in 27 hr and 30 hr than 21 hr. The developmental rate of hatched blastocyst at vitrified cleavage mouse embryos in cryo-loop was significantly higher than vitrified pronuclear embryos of control group as well as EM-grid and OPS ($p$ <0.05). The developmental rate using cryo-loop was higher than EM-grid, but in case of OPS at vitrified cleavage and mouse pronuclear embryos, no significant difference was noticed. These results of our study show that the developmental rates of mouse embryos were unaffected by various vitrification containers, but in case of mouse embryos and hatched blastocysts at late vitrified pronuclear embryos the developmental rates were higher than early vitrified pronuclear embryos. Moreover, the developmental rate of hatched blastocyst at vitrified cleavage mouse embryos was significantly higher than vitrified pronuclear embryos. For better execution of this study, it will be mandatory to include improvement of vitrification containers, cryopreservation methods and conditions, higher survival rate, safe preservation, contamination and embryo loss.

• 한국빅데이터학회지
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• 제7권1호
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• pp.49-61
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• 2022

스포츠 분야에서는 팀 전략 구상과 마케팅 등 팀 운영에 있어서, 데이터 분석의 비중이 점점 더 커지고 있다. 특히, 한국프로야구에서는 한 시즌이 끝나면 FA, 트레이드 등 다음 해 팀 전략을 구상하기 위해서 선수 영입과 선수 육성 등의 계획을 수립하는데, 이 때 선수들의 다음 해 성적을 예측하는 것이 매우 중요하다. 본 연구에서는 타자만으로 대상을 한정지어 다음 해의 성적이 상승할지를 예측해보고자 하였다. 상승 및 하락의 기준이 되는 기록으로는, 계산하기 쉽고 팀 득점과의 관계가 높은 OPS로 하였다. 본 연구에서 데이터는 한국프로야구 1982년부터 2021년까지 40년간의 정규시즌 데이터를 사용하였고, 실험 방법으로는 11개의 머신러닝 분류 모델을 사용하였다. OPS의 상승 및 하락 여부를 예측해본 결과, RBF SVM, Neural Net, Gaussian Process, AdaBoost가 다른 분류 모델에 비해 정확도가 높게 나왔고 나이는 정확도에 큰 영향을 주지 못했다.

• Journal of Animal Science and Technology
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• 제58권3호
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• pp.13.1-13.7
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• 2016

Background: In the present study, various freezing containers were tested for mouse embryos of respective developmental stages; embryos were vitrified and then their survival rate and developmental rate were monitored. Mouse two cell, 8 cell, and blastula stage embryos underwent vitrification freezing-thawing and then their recovery rate, survival rate, development rate, and hatching rate were investigated. Methods: EM-grid, OPS, and cryo-loop were utilized for vitrification freezing-thawing of mouse embryos. Results: It was found that recovery rate and survival rate were higher in the group of cryo-loop compared to those of EM-grid (p < 0.05). Embryonic development rate, two cell embryos to blastocyst, as well as hatching rate were higher in the control group compared to the EM-grid group and OPS group (p < 0.05), yet no difference was noted between the control group and cryo-loop group. Development rate and hatching rate of eight cell morulae and blastocysts were all lower in the treatment groups than the control group whilst hatching rate of blastocysts was higher in the control group compared to the groups of EM-grid and OPS (p < 0.05); although the cryo-loop group was shown to be slightly higher than other groups, it was not statistically significant. Conclusions: In the study, we investigate effects of freezing containers on vitrified embryos of respective developmental stages; it was demonstrated that higher developmental rate was shown in more progressed (or developed) embryos with more blastomeres. There was however, no difference in embryonic development rate was shown amongst containers. Taken together, further additional studies are warranted with regards to 1) manipulation techniques of embryos for various vitrification freezing containers and 2) preventive measures against contamination via liquid nitrogen.