• 제목/요약/키워드: ON cell

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Matlab을 이용한 망막신경절세포 감수야 구성 (Reconstruction of Receptive Field of Retinal Ganglion Cell Using Matlab)

  • 예장희;진계환;구용숙
    • 한국의학물리학회지:의학물리
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    • 제17권4호
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    • pp.260-267
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    • 2006
  • 개개의 망막신경절세포는 자신이 담당하고 있는 망막의 특정부위에 빛자극이 가해지면 그 빛자극의 특징을 활동전위의 형태로 인코딩하게 된다. 이때 개개의 망막신경절세포가 담당하고 있는 망막의 특정부위를 감수야(receptive field)라 부른다. 그러므로 망막신경절세포의 전기적 특성을 파악하기 위해서는 감수야의 위치를 규정하는 작업이 반드시 필요하다. 그 이유는 감수야의 배열 상태를 알게 되면 신경절세포가 어떻게 시각자극을 인코딩하는지 그 메커니즘에 관한 통찰이 가능하기 때문이다. 본 논문에서는 무작위 바둑판 자극을 MEA의 개개 채널에 독립적으로 인가함과 동시에 여러 망막신경절세포의 흥분파를 기록하였다. 이후 오프라인에서 망막신경절세포의 파형을 추출하고 ON-cell, OFF-cell, ON/OFF-cell로 분류한 후 개개의 망막신경절세포의 감수야를 WATLAB을 이용하여 구현하여 보았다. 이런 방식으로 재구성된 ON-cell과 OFF-cell의 감수야의 예를 제시한다.

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자궁경부암(子宮頸部癌)과 유방암(乳房癌)에 대한 삼릉(三稜)의 세포자멸사 연구 (Study of Apoptosis by Scirpi Tuber in Hela Cell and MCF-7 Cell)

  • 류갑순;이진무;이창훈;장준복;이경섭
    • 대한한방부인과학회지
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    • 제24권3호
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    • pp.1-13
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    • 2011
  • Objectives: This study was designed to investigate the analysis of apoptosis by Scirpi Tuber in Hela cell and MCF-7 cell. Methods: For cytotoxic effect of Scirpi Tuber extract, Scirpi Tuber extract were cultured on NIH3T3 cell in vitro. After treatment with various concentration of Scirpi Tuber, cell growth was evaluated in Hela cell and MCF-7 cell. Hoechst 33342 staining was performed to estimate DNA fragment effect of Scirpi Tuber on the apoptosis in Hela cell and MCF-7 cell. Annexin V/PI apoptosis assay was used to estimate the effects of Scirpi Tuber on the early apoptosis in Hela cell MCF-7 cell. All the stained cells were analyzed by a FACS. RT-PCR was used to estimate the apoptosis gene expression effect of Scirpi Tuber extract on Hela cell and MCF-7 cell. Results: Cytotoxic effect of Scirpi Tuber extract was not found on per NIH3T3 cell. The viability of Hela cell was significantly decreased Scirpi Tuber (500, $1000{\mu}g/m\ell$) in Hela cell 1day, 3day and 5days after treatment (p<0.01). The viability of MCF-7 cell was significantly decresed Scirpi Tuber ($1000{\mu}g/m\ell$) in MCF-7 cell (p<0.01), Scirpi Tuber ($500{\mu}g/m\ell$) in MCF-7 cell only 3days after treatment (p<0.01). In RT-PCR analysis, after treatment of $100{\mu}g/m\ell$ of ACR extract, BCL-2 were decreased and BAX, caspase-3 were increased both in Hela cell and MCF-7 cell. DNA fragmentation was observed the Scirpi Tuber on Hela cell and MCF-7 cell. As time goes on DNA fragmentation incresed. In Annexin V/PI apoptosis assay, after treatment of $1mg/m\ell$ of Scirpi Tuber, the early apoptotic cell increased both in Hela cell and MCF-7 cell. As time goes on apoptotic cell increased. Conclusion: Scirpi Tuber appears to have considerable activity on the apoptosis in Hela cell and MCF-7 cell.

A Study On the Cooling Effect of the Floating Horizontal Solar Cell

  • Jae-hyuk Lee
    • International journal of advanced smart convergence
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    • 제12권2호
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    • pp.182-186
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    • 2023
  • In this study, we measured the power and temperature of the floating horizontal solar cell in a coastal lagoon and compared with those of ground solar cell and water platform solar cell. Because the bottom surface of the floating horizontal solar cell was contacting the water, cooling effect was expected stronger than other cells. As a result of the measurement, the power of floating horizontal cell was 11.7% higher than that of the ground cell and 15% higher than that of the water platform cell. During the measurement, it was observed that water waves were continuously flowed on the top surface of floating horizontal cell by the wind, and it could be assumed that the cooling effect occurred not only on the bottom surface of the cell but also on the top surface. In order to analyze the cooling effect and power increasing of the horizontal cell in the wave situation, we measured power and temperature of the cell while generating artificial waves in a laboratory equipped with Zenon lamp as a solar simulator. At the height of thewater surface, the power of the cell with waves was 3.7% higherthan without waves and temperature was 4.6℃ lower. At 1 cm and 2 cm below the watersurface, power of the cell with waves was decreased by 14% and 11% than without waves while temperature was same . At 3 cm below the water surface, there was no effect of waves.

가감소합향원(加減蘇合香元) 향기액이 세포활성에 미치는 영향과 항산화효과에 관한 연구 (The Effect of GagamSohabhwang-won(Jiajiansuhexiang-yuan) Essential Oil on Cell Activity and Anti Oxidation)

  • 윤여국;김근우;구병수
    • 동의신경정신과학회지
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    • 제20권3호
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    • pp.1-13
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    • 2009
  • Objectives : The glial cell, located in between the blood vessel and nerve cell, takes charge of the cell support, nutrition supply, elimination of body waste, and cell action. GagamSohabhwangwon(GGSH), a chinese traditional medicinal prescription has been used orally for the treatment of seizures, infantile, convulsion, stroke and so forth. This paper examines the effect of the GagamSohabhwangwon(GGSH) essential oil on cell activity and anti oxidation. Methods : MTT assay methods were employed to measure the cell activity based on the amount of the GagamSohabhwangwon(GGSH) essential oil by using primarily cultivated glial cell. In addition, this paper measured a viability of the glial cell after a protein active retarder control to confirm the multiplication of the cell and examined the cell extinction by the active oxygen, an extinction shielding effect with different amount of the GagamSohabhwangwon(GGSH) essential oil to observe anti oxidation. Furthermore, this paper measured a viability of the cell and phosphorylation(phosphorylation) of the protein which affects the multiplication of the glial cell. Results : When controlling the amount of the GagamSohabhwangwon(GGSH), there was a multiplication effect of the primary glial cell, the multiplication of the cell was dependent on the density of the GagamSohabhwangwon. The multiplication power of the primary glial cell was suppressed by PKA inhibiter (H89). In compliance with the active oxygen the extinction of the primary glial cell was dependent on the density of the GagamSohabhwangwon, there was a shielding effect of the cell extinction when GagamSohabhwangwon(GGSH) was preprocessed. When inducing the multiplication of the primary glial cell, phosphorylation of the Akt, BDNF, CREB, ERK and ERM were increased. Conclusions: Based on the results, GagamSohabhwangwon essential oil will have the effect which activates the nervous system cell and protects the cell through anti oxidation.

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HeLa cell과 MCF-7 cell에 대한 오가피(五加皮)의 apoptosis 효과 (Effects of Acanthopanacis Cortex Radicis on the Apoptosis in HeLa cell and MCF-7 cell)

  • 김경숙;이진무;이창훈;장준복;이경섭
    • 대한한방부인과학회지
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    • 제24권3호
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    • pp.14-27
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    • 2011
  • Objectives: This study was designed to investigate the effects of Acanthopanacis Cortex Radicis extract(ACRE) on the apoptosis in HeLa cell and MCF-7 cell. Methods: After treatment with various concentration of ACRE, cell growth was evaluated in HeLa cell and MCF-7 cell. Hoechst 33342 staining was performed to estimate DNA fragment effect of ACRE on the apoptosis in HeLa cell and MCF-7 cell. Annexin V/PI apoptosis assay was used to estimate the effects of ACRE on the early apoptosis in HeLa cell and MCF-7 cell. RT-PCR was used to estimate the apoptosis gene expression effect of ACRE on Hela cell MCF-7 cell. Results: Under $0.1mg/m\ell$ of ACRE, cytotoxic effect was not found per NIH3T3 cell. The viability of HeLa cell and MCF-7 cells was significantly decreased ACRE ($100{\mu}g/m\ell$) in HeLa cell and MCF-7 cell, ACRE ($50{\mu}g/m\ell$) in HeLa cell 3 days after treatment, in MCF-7 cell 1&3 days after treatment (p<0.01). DNA fragmentation was observed 3 days after treatment of cl of ACRE on HeLa cell and MCF-7 cell. In Annexin V/PI apoptosis assay, after treatment of $100{\mu}g/m\ell$ of ACRE, the early apoptotic cell increased both in HeLa cell and MCF-7 cell. In RT-PCR analysis, after treatment of $100{\mu}g/m\ell$ of ACRE, bcl-2 were decreased and bax, caspase-3 were increased both in HeLa cell and MCF-7 cell. Conclusions: ACRE appears to have considerable activity on the apoptosis in HeLa cell and MCF-7 cell.

VA 액정 셀의 러빙방향에 따른 패턴 확인 (Pattern Check According To The Rubbing Direction Of The LC VA Cell)

  • 김정하;김학래;송장근
    • 한국정보통신학회:학술대회논문집
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    • 한국정보통신학회 2015년도 추계학술대회
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    • pp.481-483
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    • 2015
  • LCD의 특성을 이해하기 위해 Vertical Alignment Liquid Crystal Cell을 만들어보고, 전류를 흘려 Cell에 나타나는 pattern을 확인한다. 다양한 rubbing 방향을 주어 완성된 Cell에 나타나는 여러 가지 pattern들을 확인, 비교해보고 그 pattern의 발생 원인에 대해 연구한다. Cell에 전류를 가해주면 line이 나타나는데 가해준 전압과 주파수가 높을수록 line이 많이 나타나는 것을 확인할 수 있었다.

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Every Single Cell Clones from Cancer Cell Lines Growing Tumors In Vivo May Not Invalidate the Cancer Stem Cell Concept

  • Li, Fengzhi
    • Molecules and Cells
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    • 제27권4호
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    • pp.491-492
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    • 2009
  • We present the result of our research on the tumorigenic ability of single cell clones isolated from an aggressive murine breast cancer cell line in a matched allografting mouse model. Tumor formation is basically dependent on the cell numbers injected per location. We argue that in vivo tumor formation from single cell clones, isolated in vitro from cancer cell lines, may not provide conclusive evidence to disprove the cancer stem cell (CSC) theory without additional data.

Effects of Non-Uniform Traffic Distribution on the Capacity of Reverse Link CDMA System

  • Cho, Choon-Geun;Ann, Jong-Hoon;Tchah, Kyun-Hyon
    • 한국통신학회논문지
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    • 제25권12A호
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    • pp.1828-1835
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    • 2000
  • In this paper, we analyzed the other-cell interference characteristics for various non-uniform traffic distributions and their effects on the capacity of multi-cell CDMA system. We consider three different traffic distributions, i.e., linear, exponential and Gaussian traffic distribution with distribution parameters. Changing the distribution parameter, we can obtain the center-focused distributions or uniform distributions for each model. From the results of other-cell interference calculation we can see that the other-cell interference decreases, as the user concentrates on the base station. Also using frequency reuse efficiency indicating the capacity reduction of a multi-cell system when compared to a single cell system, we evaluate the effect of traffic distribution on the reverse link CDMA capacity. For linear case, the capacity of multi-cell system is reduced to 0.637∼0.867 times that of single cell system. On the other hand, for both exponential and Gaussian cases, the capacity under a multi-cell environment is equal to 70∼100% of that under a single cell. Therefore, we conclude that the average capacity of multi-cell CDMA system are increased when users are likely to be at near the cell base station due to reduced total other-cell interference and decreased when users exist at near the cell edge regardless of traffic distribution models.

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Kinetic Analysis of the Effect of Cell Density on Hybridoma Cell Growth in Batch Culture

  • Lee, Eun-Yeol
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제7권2호
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    • pp.117-120
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    • 2002
  • The effect of cell density on cell growth was investigated in a suspension batch culture of hybridoma cells. The specific growth rate was found to increase with increasing initial cell density and then to decrease with further increases in initial cell density. In order to quantitatively describe the dependence of specific growth rate on cell density, a kinetic model is proposed, which satisfactorily represents the experimental data.

셀 전송비율에 의한 우선순위 제어방식을 사용한 ATM 스위치의 성능 분석 (Performance Analysis of ATM Switch Using Priority Control by Cell Transfer Ratio)

  • 박원기;김영선;최형진
    • 전자공학회논문지A
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    • 제32A권12호
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    • pp.9-24
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    • 1995
  • In this paper, we proposed and analysed two kinds of priority control mechanism to archive the cell loss rate requirement and the delay requirement of each class. The service classes of our concern are the high time priority class(class 1) and the high loss priority class(class 2). Two kinds of priority control mechanism is divided by the method of storing the arriving class 2 cell in buffer on case of buffer full. The first one is the method which discarding the arriving class 2 cell, the second one is the mothod which storing the arriving class 2 cell on behalf of pushing out the class 1 cell in buffer. In the proposed priority schemes, one cell of the class 1 is transmitted whenever the maximum K cells of the class 2 is transmitted on case of transmitting the class 1 cell and the class 2 cell sequentially. In this paper, we analysed the cell loss rate and the mean cell delay for each class of the proposed priority scheme by using the Markov chain. The analytical results show that the characteristic of the mean cell delay becomes better for the class 1 cell and that of the cell loss rate becomes better for the class 2 cell by selecting properly the cell transfer ratio according to the condition of input traffic.

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