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Effects of Probiotic Supplementation on Growth Performance, Blood Metabolites, and Meat Quality of Hanwoo Steer (혼합 생균제 급여가 거세한우의 성장, 혈액성상 및 육질에 미치는 영향)

  • Paradhipta, Dimas H.V.;Jeon, Chung-Hwan;Choi, In-Hag;Lee, Hyuk-Jun;Joo, Young-Ho;Lee, Sung-Shin;Kim, Dong-Hyeon;Kim, Sam-Churl
    • Journal of Environmental Science International
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    • v.26 no.8
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    • pp.967-976
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    • 2017
  • In this study, the effect of probiotic supplementation on growth performance, blood metabolites, and meat quality of Hanwoo steer was investigated. A total of 32 Hanwoo steers (15-17 months, average body weight $462{\pm}37.9kg$) were randomly allotted to 4 dietary treatments (0, 0.5, 1.0, and 1.5% mixed probiotics), with four Hanwoo steers per pen (two replicates per treatments), and reared for 12 months. There were no differences among treatments in growth performance of Hanwoo steer (P>0.05); however, feed intake decreased linearly with increasing levels of mixed probiotics. Growth hormone and Blood Urea Nitrogen (BUN) levels responded linearly with increasing levels of dietary mixed probiotics (P<0.05), but not insulin and blood glucose did not. In particular, total cholesterol was significantly lower for the 1% mixed probiotic treatment in comparison with that of the other treatments (P<0.05). The pH, Thiobarbituric Acid Reactive Substances (TBARS), cooking loss, and meat color were influenced by increasing levels of mixed probiotics (P<0.05), but the carcass characteristics and shear force were not. Regarding sensory evaluation, the addition of mixed probiotics resulted in significant difference in meat color, tenderness, aroma, off-flavor, juiciness, and marbling score, but not in overall acceptability. In addition, fatty acid profiles indicated no differences between control and mixed probiotic treatments. In conclusion, mixed probiotic treatment at 1% levels can enhance consumer preferences possibly by reducing cholesterol and TBARS.

A Study on Scientific Thinking of Korean High School Students-With Reference to Logical Thinking and Reasoning Power- (고교생의 과학적 사고력에 관한 연구-논리적 사고와 추리력을 중심으로-)

  • Hwang, Keung-Yeon;Park, In-Keun;Kim, Tai-Sung
    • Journal of The Korean Association For Science Education
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    • v.9 no.1
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    • pp.19-37
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    • 1989
  • The purpose of this study was to investigate the development of logical thinking and scientific reasoning pattern of Korean high school students. To carry out this study subjects were selected about 2,000 Junior high school students, and about 4,100 senior high school students throughout the nation. They were identified as concrete, transitional or formal operational stage with the use of TOLT(the Test of Logical Thinking) by Tobin and Capie(1980), and TOSR(the Test of Scientific Reasoning) by W.A Farmer(1986). This study turned out that more than 76% of Junior high school students were classified as the concrete operational stage and about 44% of senior high school students were classified as the formal operational stage, while about 26% of them were still in the concrete operational level. This study showed that the main factor of the intellectual development of students is learning by the gradual advancement of their grades and especially entrance into the senior high school rather than by the physical growth. This study also showed that there are the take-off stage of the development of logical thinking between fourteen and fifteen years of their ages. Less than 25% of junior high school students were in the formal operational stages which are capable of control of variables, probabilistic, correlation and combinational logic in problem-solving situation, while 33-54% of senior high school students were in the formal operational levels. 38% of junior high school students were in the formal operational stage which is capable of proportional logic, while about 55% of senior high school students were in the formal operational stage. Less than 20% of senior high school students were classified as group of highly capable of scientific reasoning, while more than 23% of them were classified as group of poor capability. It also turned out that there are differences or no differences between male and female students of each school in problem-solving situation regarding each logic approach. These differences were proved to be fluctuating depending on the situations and their grades. The other results of this study is similar to those of other researches such as Tomlinson-Keasey 1972, Coleman 1973, Lawson 1973, Lawson and Renner 1974, Neimark 1975, Han 1982, and Kim 1989.

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Structural and Functional Analysis of Nitrogenase Fe Protein with MgADP bound and Amino Acid Substitutions (MgADP 결합 및 아미노산 치환 Nitrogenase Fe 단백질의 구조 및 기능 분석)

  • Jeong, Mi-Suk;Jang, Se-Bok
    • Journal of Life Science
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    • v.14 no.5
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    • pp.752-760
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    • 2004
  • The function of the [4Fe-4S] cluster containing iron (Fe-) protein in nitrogenase catalysis is to serve as the nucleotide-dependent electron donor to the MoFe protein which contains the sites for substrate binding and reduction. The ability of the Fe protein to function in this manner is dependent on its ability to adopt the appropriate conformation for productive interaction with the MoFe protein and on its ability to change redox potentials to provide the driving force required for electron transfer. The MgADP-bound (or off) conformational state of the nitrogenase Fe protein structure described reveals mechanisms for long-range communication from the nucleotide-binding sites to control affinity of association with the MoFe protein component. Two pathways, termed switches I and II, appear to be integral to this nucleotide signal transduction mechanism. In addition, the structure of the MgADP bound Fe protein provides the basis for the changes in the biophysical properties of the [4Fe-4S] observed when Fe protein binds nucleotides. The structures of the nitrogenase Fe protein with defined amino acid substitutions in the nucleotide dependent signal transduction pathways of the Switch I and Switch II have been determined by X-ray diffraction methods. These two pathways have been also implicated by site directed mutagenesis studies, structural analysis and analogies to other proteins that utilize similar nucleotide dependent signal transduction pathways. We have examined the validity of the assignment of these pathways in linking the signals generated by MgATP binding and hydrolysis to macromolecular complex formation and intermolecular electron transfer. The results provide a structural basis for the observed biophysical and biochemical properties of the Fe protein variants and interactions within the nitrogenase Fe protein-MoFe protein complex.

Physicochemical and Sensory Characteristics of Rice Cookies Based on Goami 2 with Sesames(White and Black) and Perilla Seeds (흰깨, 검은깨 및 들깨가 고아미 2호를 이용한 쌀쿠키의 이화학적 및 관능적 특성에 미치는 영향)

  • Jung, Young-Jeong;Seo, Han-Seok;Myung, Jyong-Eun;Shin, Jeong-Min;Lee, Eun-Ju;Hwang, In-Kyeong
    • Korean journal of food and cookery science
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    • v.23 no.6
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    • pp.785-792
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    • 2007
  • The aim of this study was to develop rice cookies based on 'Goami 2' rice, and made with sesame (white and black) and perilla seeds. Furthermore, we studied the physicochemical and sensory characteristics of the cookies as well as the alternative effects of the sesames or perilla seeds for butter, where we substituted the sesames or perilla for 30% of the total butter. The pH values of the cookie doughs made with the sesames or perilla seeds were significantly different (p<0.001), whereas the spread factors of the doughs were not significantly different. The Hunter color L-, a-, and b- values were significantly different for the cookies made with the added sesames or perilla seeds (p<0.001). The brittleness of the Goami 2 rice cookies (control) was significantly higher than that of the cookies made with the sesames or perilla (p<0.001). In a descriptive analysis performed by 8 trained panelists, the following sensory characteristics: yellow, black, sweet taste, nutty taste, nutty odor, oily flavor, and off flavor, were significantly different among the cookie samples. In a consumer test by 50 women students, the Goami 2 rice cookies were preferred in the following order: Goami 2 rice cookies with no additions > black sesame cookies > white sesame cookies > perilla cookies. In conclusion, some physicochemical and sensory characteristics of the cookies were different, in relation to being made with or without the sesame or perilla seeds. However, because of the small difference in acceptance among the cookies, the sesame and perilla rice cookies show strong potentials for consumer acceptance. Moreover, the black sesame cookies were more acceptable in terms of butter substitution for the cookie preparation.

Inhibition Effect of Enzymatic Hydrolysate from Japanese Mud Shrimp Upogebia major on TNF-α-induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells (HUVECs) (혈관내피세포에서 TNF-α로 유도되는 혈관염증에 대한 쏙(Upogebia major) 효소가수분해물의 억제 효과)

  • Kim, So-Yeon;Yang, Ji-Eun;Song, Jae-Hee;Maeng, Sang-Hyun;Lee, Ji-Hyun;Yoon, Na-Young
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.2
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    • pp.127-134
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    • 2018
  • Arteriosclerosis is the major cause of coronary artery and cerebrovascular disease, which are leading causes of death. Pro-inflammatory cytokines induce injury to vascular endothelial cells by increasing cell adhesion molecules, leading to vascular inflammation, a major risk factor for the development of arteriosclerosis. In the current study, we investigated the inhibitory effect of enzymatic hydrolysate from Japanese mud shrimp Upogebia major on the inflammation of tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$)-stimulated human umbilical vein endothelial cells (HUVECs). We first evaluated the antioxidant and angiotensin I-converting enzyme (ACE) inhibitory activities of eight U. major enzymatic hydrolysates: alcalase, papain, ${\alpha}$-chymotrypsin (${\alpha}-Chy$), trypsin, pepsin, neutrase, protamex and flavourzyme. Of these, ${\alpha}-Chy$ exhibited potent antioxidant and ACE inhibitory activities. The ${\alpha}-Chy$ hydrolysate was fractionated by two ultrafiltration membranes of 3 and 10 kDa. The ${\alpha}-Chy$ hydrolysate of U. major and its molecular weight cut-off fractions resulted in a significant reduction in NO production and a decrease in cell adhesion molecules [vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1) and endothelial-selectin (E-selectin)] and pro-inflammatory cytokines [interleukin-6 (IL-6), interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1)] in $TNF-{\alpha}$-stimulated HUVECs. These results suggest that enzymatic hydrolysate from U. major can be used in the control and prevention of vascular inflammation and arteriosclerosis.

Characteristics of Dissimilar Materials Al alloy(A6005)-Mg alloy(AZ61) Under Friction Stir Welding for Railway Vehicle (철도차량 적용을 위한 Al alloy(A6005)-Mg alloy(AZ61) 이종소재 마찰교반용접 특성 연구)

  • Lee, Woo-Geun;Kim, Jung-Seok;Sun, Seung-Ju;Lim, Jae-Yong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.8
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    • pp.706-713
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    • 2016
  • In this study, the welding characteristics of friction stir welding were investigated in accordance with the tool plunge position and cooling to the base materials for the joining of dissimilar materials (A6005-AZ61). Other different welding conditions, such as the tool rotation speed and welding speed, were fixed to 500rpm-30mm/min, respectively, and welding was then carried out by placing the Mg alloy (AZ61) on the advancing side and Al alloy(A6005) on the retreating side. Welding was conducted under six different conditions. To investigate the welding characteristic, tensile test and microstructure observations using an optical microscope were carried out. As the tensile test result, the maximum strength appeared under the condition in which the tool is moved 1 mm to the Mg alloy direction and cooling to the base materials. Under the same welding conditions, the strength with cooling was approximately two times higher than that without cooling. The tool was located in each direction of 1.7 mm from the weld line. Therefore, in the excessive off-set of tool position, the welding integrity was in an extremely poor condition due to the lack of stirring. This study was confirmed by the A6005-AZ61 dissimilar friction stir welding the welding speed and the tool rotation speed. In addition, the temperature control and tool plunge position are important welding parameters.

Design and Analysis of Ubiquitous Social Network Management Service Model: u-Recruiting Service Model (유비쿼터스 사회연결망관리 서비스 모델 설계 및 분석: u-구인 구직 서비스 모델을 중심으로)

  • Oh, Jae-Suhp;Lee, Kyoung-Jun;Kim, Jae-Kyeong
    • Information Systems Review
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    • v.13 no.1
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    • pp.33-59
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    • 2011
  • Although online social network services widely used in human networking and recruiting industries, it is showing off its limitations in followings-it's hard to reach the status of seamless connection between offline and online; the incompletion and low credibility of the information came from non-face-to-face profile exchange; and the restraint of user autonomy due to centralized control. This paper defines the ubiquitous social network management which enables the seamless real-time face-to-face social interactions of the users based on WPAN (Wireless Personal Area Network) who share the same interest in real word and deduces a ubiquitous social network management framework based on it. As an instance of ubiquitous social network management, u-Recruiting service model will be designed and analyzed. The Analysis using the business model will be followed by the possible scenario of service model. The role, value proposition and potential benefits of the each participants in this service model and will be given as well. In order to evaluate relative advantages of the model suggested by this study, 6 cases will be compared.

A study on the Shelf-life Extension of Minimally Processed Carrot (최소가공 당근의 유통기한 연장 연구)

  • Lee, Kyoung-Hae;Lim, Hui-Kyoung
    • Journal of Food Hygiene and Safety
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    • v.23 no.4
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    • pp.330-337
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    • 2008
  • Betarich carrots were washed at various hypochloric acid(HCA) concentration and steeping time and packed in PE bag keeping at $8^{\circ}C$ for 12 days, in order eventually to examine microbiology, firmness, surface color, whiteness index(WI) and sensory quality. It was found that total bacterial counts at T-II and T-III with the 3 minute steeping were $4.37{\pm}0.19\;\log\;CFU/g$ and $4.27{\pm}0.13\;\log\;CFU/g$ respectively, showing slight decrease compared to control condition. E. coli was not detected at all treatments but less coliforms were detected at the 8-day treatments of T-II and T-III. Treatment of 3-minute steeping showed smaller reduction in firmness than that of I-minute steeping at 12-days, and T-1 (T-3) had the largest (smallest) reduction among them. It was also found that during the treatment the L-value showed decreasing trend, but the parameter a- and b-value showed increasing trend. WI increased, and its change was small with the increase in HCA concentration. The sensory quality check after 8 day storage showed that evaluation of the off-flavor appeared to be significantly high (p<0.05) at both non-treatment and HCA treatment. On the basis of the results above, it is likely to be more effective to prolong the period of circulation of beta rich carrots if you use HCA over 50 ppm for washing betarich carrots. This study will contribute to improve safety and quality in circulation of beta rich carrots.

Random Balance between Monte Carlo and Temporal Difference in off-policy Reinforcement Learning for Less Sample-Complexity (오프 폴리시 강화학습에서 몬테 칼로와 시간차 학습의 균형을 사용한 적은 샘플 복잡도)

  • Kim, Chayoung;Park, Seohee;Lee, Woosik
    • Journal of Internet Computing and Services
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    • v.21 no.5
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    • pp.1-7
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    • 2020
  • Deep neural networks(DNN), which are used as approximation functions in reinforcement learning (RN), theoretically can be attributed to realistic results. In empirical benchmark works, time difference learning (TD) shows better results than Monte-Carlo learning (MC). However, among some previous works show that MC is better than TD when the reward is very rare or delayed. Also, another recent research shows when the information observed by the agent from the environment is partial on complex control works, it indicates that the MC prediction is superior to the TD-based methods. Most of these environments can be regarded as 5-step Q-learning or 20-step Q-learning, where the experiment continues without long roll-outs for alleviating reduce performance degradation. In other words, for networks with a noise, a representative network that is regardless of the controlled roll-outs, it is better to learn MC, which is robust to noisy rewards than TD, or almost identical to MC. These studies provide a break with that TD is better than MC. These recent research results show that the way combining MC and TD is better than the theoretical one. Therefore, in this study, based on the results shown in previous studies, we attempt to exploit a random balance with a mixture of TD and MC in RL without any complicated formulas by rewards used in those studies do. Compared to the DQN using the MC and TD random mixture and the well-known DQN using only the TD-based learning, we demonstrate that a well-performed TD learning are also granted special favor of the mixture of TD and MC through an experiments in OpenAI Gym.

Fabrication of passive-aligned optical sub-assembly for optical transceiver using silicon optical bench (실리콘 광학벤치를 사용한 수동정렬형 광송수신기용 광부모듈의 제작)

  • Lee, Sang-Hwan;Joo, Gwan-Chong;Hwang, nam;moon, Jong-Tae;Song, Min-Kyu;Pyun, Kwang-Eui;Lee, Yong-Hyun
    • Korean Journal of Optics and Photonics
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    • v.8 no.6
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    • pp.510-515
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    • 1997
  • Packaging takes an extremely important element of optical module cost due primarily to the added complication of alignment between semiconductor devices and optical fiber, and many efforts have been devoted on reducing the cost by eliminating the complicated optical alignment procedures in passive manner. In this study, we fabricated silicon optical benches on which the optical alignments are accomplished passively. To improve the positioning accuracy of a flip-chip bonded LD, we adopted fiducial marks and solder dams which are self-aligned with V-groove etch patterns, and a stand-off to control the height and to improve the heat dissipation of LD. Optical sub-assemblies exhibited an average efficiency of -11.75$\pm$1.75 dB(1$\sigma$) from the LD-to-single mode fiber coupling and an average sensitivity of -35.0$\pm$1.5 dBm from the fiber and photodetector coupling.

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