• Bulletin of the Korean Chemical Society
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• v.29 no.7
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• pp.1311-1314
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• 2008

Bacillus halodurans O-methyltransferase (BhOMT) is an S-adenosylmethionine dependent methyltransferase. In our previous study, three dimensional structure of the BhOMT has been determined by comparative homology modeling and automated docking study showed that two hydroxyl groups at 3'- and 4'-position in Bring and structural rigidity of C-ring resulting from the double bond characters between C2 and C3 of flavonoid, were key factors for interaction with BhOMT. In the present study, BhOMT was cloned and expressed. Binding assay was performed on purified BhOMT using fluorescence experiments and binding affinity of luteolin, quercetin, fisetin, and myricetin were measured in the range of $10^7$. Fluorescence quenching experiments indicated that divalent cation plays a critical role on the metal-mediated electrostatic interactions between flavonoid and substrate binding site of BhOMT. Fluorescence study confirmed successfully the data obtained from the docking study and these results imply that hydroxyl group at 7-position of luteolin, quercetin, fisetin, and myricetin forms a stable hydrogen bonding with K211 and carboxyl oxygen of C-ring forms a stable hydrogen bonding with R170. Hydroxyl group at 3'-and 4'-position in the B-ring also has strong $Ca^{2+}$ mediated electrostatic interactions with BhOMT.

• The Journal of Internal Korean Medicine
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• v.21 no.4
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• pp.565-573
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• 2000

Objective : This research was carried out to find out the general understanding of allergic diseases, the frequencies of allergic diseases and the attitude toward their oriental medical treatment(OMT) among common people. Methods : A total of 360 visitors at The 2nd International Exhibition on Oriental Medicine in 2000, participated in this research. We performed this survey with 22 items on the questionnaire. Results : Respondents knew the. fact that the allergic diseases were hereditary and could be affected by the living environment. 41.8% of the respondents had a past history of allergic diseases. Among the people who had allergic diseases, 63.5% had allergic rhinitis, which was the largest portion. Among them, 52.9% had a familial history. 81.5% of respondents with allergic diseases received western medical treatment for their allergies, 16.3% received both western and oriental medical treatment and 2.2% received OMT only. Among those who were treated for allergic disease, 62.0% were unsatisfied with the low effect of the treatment. To the point of excellence of OMT, 69.7%, which was the largest portion of respondents, believed that OMT could improve their constitutions. The problem with OMT was the high price of herbal medicine and this was the problem most frequently stated, by 51.4% of respondents. There was a positive attitude towards the effect of OMT, and respondents had a preference to receive OMT in the future(p<0.01). Conclusions : These days, it is a fact that the OMT for allergic diseases has a lower proportion to that of western treatment In order to increase the role of OMT, we think, oriental medical doctors need to develop an explanatory model of improving $people^{\circ}{\phi}s$ constitutions and should lower the cost of herbal medicine.

• Journal of Microbiology and Biotechnology
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• v.16 no.7
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• pp.1090-1096
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• 2006

O-Methylation is a common modification reaction found in nature, and is mediated by an O-methyltransferase (OMT). OMTs have been mainly studied in plants, whereas only a few OMTs have been studied in microbes. When searching the Bacillus cereus genome, four putative small molecular OMTs were identified, among which BcOMT-1 was cloned and expressed in E. coli as a his-tag fusion protein. The whole cell expressing BcOMT-1 was used to methylate several flavonoids. Eriodictyol, luteolin, quercetin, and taxifolin, all of which contain 3' and 4' hydroxyl groups, served as methyl group acceptors for BcOMT-1, whereas naringenin, apigenin, 3,3'-dihydroxyflavone, and 3,4'-dihydroxyflavone did not function as substrates. Analysis of the reaction products using HPLC showed two different peaks, and NMR revealed that the methylation position was at the hydroxyl group of either carbon 3' or 4'. Therefore, this showed that BcOMT-1 used flavonoids containing ortho hydroxyl groups and transferred a methyl group to either of two hydroxyl groups.

• The Journal of Korean Medicine
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• v.26 no.3 s.63
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• pp.124-134
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• 2005

Objectives : This study was peformed to evaluate the actual state and the effects of Smoking Prevention or Stop-Smoking Programs (Sp/SSP) for middle school students and to confirm students' needs for Oriental Medical Treatments (OMT) in SP/SSP. Methods : Thirty-nine middle school students who took part in the SP/SSP of the Public Health Center (PHC) in July 2004 answered the questionnaire. There were twenty students in $PHC{\alpha}$, and 19 students in $PHC{\beta}$. Resillts : 1. At the elementary school, SP/SSP is not enough, compared with middle school (p=.000). 2. Subjects preferred tile documents from PCH to those from school $(PCH{\alpha}: p-value=.002,\;PCH{\beta}: p-value=.004)$ 3. There was no mention of OMT in school SP/SSP. 4. The degree of thinking about need of teaming OMT in SSP has a positive correlationship (p-value=.000) with intention to be treated with auricular acupuncture therapy. Results : The SP/SSP for middle-school students must be improved to be more active, more experimental and more individual. Also, a more various, positive approach in OMT is required toward the Stop-Smoking Program.

• Journal of Microbiology and Biotechnology
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• v.16 no.4
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• pp.619-622
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• 2006

Biotransformation is a good tool to synthesize regioselective compounds. It could be performed with diverse sources of genes, and microorganisms provide a myriad of gene sources for biotransformation. We were interested in modification of flavonoids, and therefore, we cloned a putative O-methyltransferase from Bacillus cereus, BcOMT-2. It has a 668-bp open reading frame that encodes a 24.6-kDa protein. In order to investigate the modification reaction mediated by BcOMT-2, it was expressed in E. coli as a His-tag fusion protein and purified to homogeneity. Several substrates such as naringenin, luteolin, kaempferol, and quercetin were tested and reaction products were analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). BcOMT-2 could transfer a methyl group to substrates that have a 3' functional hydroxyl group, such as luteolin and quercetin. Comparison of the HPLC retention time and UV spectrum of the quercetin reaction product with corresponding authentic 3'-methylated and 4'-methylated compounds showed that the methylation position was at either the 3'-hydroxyl or 4'-hydroxyl group. Thus, BcOMT-2 transfers a methyl group either to the 3'-hydroxyl or 4'-hydroxyl group of flavonoids when both hydroxyl groups are available. Among several flavonoids that contain a 3'- and 4'-hydroxyl group, fisetin was the best substrate for the BcOMT-2.

• Proceedings of the IEEK Conference
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• 2002.06a
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• pp.363-366
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• 2002

In this paper, we present a Ku-band OMT(orthomode transducer) structure employing a simple waveguide transition withou a septum. Various aspects of the OMT design are discussed. The design is carried out using a commercial software HFSSe The designed OMT shows good characteristics over 12.5114.25 CHz frequency bands.

• Journal of the Korean Society for information Management
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• v.14 no.2
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• pp.165-189
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• 1997

In analyzing, designing, implementing the system, the traditional modeling method lacks design procedure and specification in reflecting the functional or nonfunctional requirements perfectly and consequently. These problems are unreliable in the Library and Information System since it requires a diversity of reference methodes based on various types of data. In this paper, we modify the method partially which the OMT proposes and apply it on system development. We also prepare the object oriented modeling modified from OMT type' modeling for Library User System Requirements.

• Journal of Oriental Neuropsychiatry
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• v.19 no.2
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• pp.313-320
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• 2008

The purpose of this study was to investigate the clinical application of oriental medical therapy and Art-therapy to the panic disorder patient. We treated the patient with OMT(oriental medical therapy) and Art-Therapy. Art-Therapy helped the patient to solve the problems related with her family and OMT helped to improve the patient's symptoms. The application of Art-Terapy and OMT has positive effects on the patient with panic attack.

• Journal of Oriental Neuropsychiatry
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• v.16 no.1
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• pp.201-209
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• 2005

The purpose of this study was to investigate the clinical application of oriental medical therapy(OMT) and Myers-Briggs Type Indicator(MBTI) to Bipolar disorder. We treated the patient with OMT and Counseling. Counseling with the MBTI helped the patient to solve the life stress induced by the spouse conflict. Following the treatment, the patient's symptoms(somatic & mental) were improved. The applicability of Counseling with the MBTI has positive effects on the patient with Bipolar disorder.

• Journal of Positioning, Navigation, and Timing
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• v.12 no.4
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• pp.431-436
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• 2023

In order to provide continuous Korea Augmentation Satellite System (KASS) services, the operation organization should continuously and systematically perform operations, maintenance, and technical activities. All subsystems of KASS, all Line Replacement Units (LRUs), and Software Replacement Units (SWRUs) should be operated and maintained continuously. In order to effectively manage these activities, it is necessary to develop an Operation Management Tool (OMT). For this purpose, operation management, operation organization, and operation activities were defined. The defined requirements were divided into general, function, interface, and database for OMT development, and functions and decision-making procedures consisting of site/asset/trouble/work order/schedule management were designed. The OMT developed by reflecting the site and asset information as well as the design content met predefined requirements. The OMT will be actively used in actual KASS operation and will evolve by continuously reflecting the additional requirements of operators. Through this tool, KASS will support continuous service provision.