• Title/Summary/Keyword: OEP

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Molecular Cloning and Characterization of Outer Envelope Membrane Protein from Salicornia herbacea (퉁퉁마디로부터 색소체 외막 단백질 유전자의 분리 및 발현분석)

  • Ermawati Netty;Cha, Joon-Yung;Liang, Yingshi;Jung, Min-Hee;Shin, Dongjin;Lee, Byung-Hyun;Lee, Kon-Ho;Son, Daeyoung
    • Journal of Plant Biotechnology
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    • v.31 no.4
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    • pp.273-278
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    • 2004
  • Complementary DNA encoding chloroplast outer envelope membrane protein (OEP) from the halophyte Salicornia herbacea has been cloned and sequenced. The full length cDNA is 596 bp and encodes a polypeptide of 91 amino acid residues with a molecular mass of 8.9 kDa. The expression level of ShOEP increased by salt, drought and ABA treatments. ShOEP expression was largely induced in roots and shoots by high salts. The biological function of ShOEP was examined by yeast complementation. ShOEP can suppress Na$^{+}$ sensitivity of yeast mutant (cnb$\Delta$) in the presence of salt. These results suggest that ShOEP is a salt inducible gene and may have functions in the regulation of plant salt stress.ant salt stress.

Improved Original Entry Point Detection Method Based on PinDemonium (PinDemonium 기반 Original Entry Point 탐지 방법 개선)

  • Kim, Gyeong Min;Park, Yong Su
    • KIPS Transactions on Computer and Communication Systems
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    • v.7 no.6
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    • pp.155-164
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    • 2018
  • Many malicious programs have been compressed or encrypted using various commercial packers to prevent reverse engineering, So malicious code analysts must decompress or decrypt them first. The OEP (Original Entry Point) is the address of the first instruction executed after returning the encrypted or compressed executable file back to the original binary state. Several unpackers, including PinDemonium, execute the packed file and keep tracks of the addresses until the OEP appears and find the OEP among the addresses. However, instead of finding exact one OEP, unpackers provide a relatively large set of OEP candidates and sometimes OEP is missing among candidates. In other words, existing unpackers have difficulty in finding the correct OEP. We have developed new tool which provides fewer OEP candidate sets by adding two methods based on the property of the OEP. In this paper, we propose two methods to provide fewer OEP candidate sets by using the property that the function call sequence and parameters are same between packed program and original program. First way is based on a function call. Programs written in the C/C++ language are compiled to translate languages into binary code. Compiler-specific system functions are added to the compiled program. After examining these functions, we have added a method that we suggest to PinDemonium to detect the unpacking work by matching the patterns of system functions that are called in packed programs and unpacked programs. Second way is based on parameters. The parameters include not only the user-entered inputs, but also the system inputs. We have added a method that we suggest to PinDemonium to find the OEP using the system parameters of a particular function in stack memory. OEP detection experiments were performed on sample programs packed by 16 commercial packers. We can reduce the OEP candidate by more than 40% on average compared to PinDemonium except 2 commercial packers which are can not be executed due to the anti-debugging technique.

Emission Characteristic of PtOEP Phosphor in Single- and Multi-layer Electroluminescence Devices

  • Tsuboi, Taiju;Tanigawa, Masayuki
    • 한국정보디스플레이학회:학술대회논문집
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    • 2003.07a
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    • pp.887-888
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    • 2003
  • We report the electroluminescence properties of single- and multi-layer electroluminescence devices using PtOEP phosphor. Weak emission bands with peaks at 540 and 567 nm are observed in the former and latter devices, respectively, besides the well-known 648 nm PtOEP emission. The 540 nm emission increases in proportion to the third power of current density, while the 648 nm emission band increases linearly. Discussion is made on a reason for a much smaller luminance of PtOEP compared with $Ir(ppy)_{3}$ phosphor.

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Targeting of Nuclear Encoded Proteins to Chloroplasts: a New Insight into the Mechanism

  • Lee, Yong-Jik;Kim, Yong-Woo;Pih, Kyeong-Tae;Hwang, Inhwan
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.5
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    • pp.407-409
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    • 2000
  • Outer envelope membrane proteins of chloroplasts encoded by the nuclear genome are transported without the N-terminal transit peptide. Here, we investigated the targeting mechanism of AtOEP7, an Arabidopsis homolog of small outer envelope membrane proteins in vivo. AtOEP7 was expressed transiently in protoplasts or stably in transgenic plants as fusion proteins with GFP. In both cases AtOEP7:GFP was targeted to the outer envelope membrane when assayed under a fluorescent microscope or by Western blot analysis. Except the transmembrane domain, deletions of the N- or C-terminal regions of AtOEP7 did not affect targeting although a region closed to the C-terminal side of the transmembrane domain affected the targeting efficiency. Targeting experiments with various hybrid transmembrane mutants revealed that the amino acid sequence of the transmembrane domain determines the targeting specificity The targeting mechanism was further studied using a fusion protein, AtOEP7:NLS:GFP, that had a nuclear localization signal. AtOEP7:NLS:GFP was efficiently targeted to the chloroplast envelope despite the presence of the nuclear localization signal. Taken together, these results suggest that the transmembrane domain of AtOEP7 functions as the sole determinant of targeting specificity and that AtOEP7 may be associated with a cytosolic component during translocation to the chloroplast envelope membrane.

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De-Obfuscated Scheme for Obfuscation Techniques Based on Trampoline Code (트램폴린 코드 기반의 난독화 기법을 위한 역난독화 시스템)

  • Minho Kim;Jeong Hyun Yi;Haehyun Cho
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.33 no.6
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    • pp.1043-1053
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    • 2023
  • Malware analysts work diligently to analyze and counteract malware, while developers persistently devise evasion tactics, notably through packing and obfuscation techniques. Although previous works have proposed general unpacking approaches, they inadequately address techniques like OEP obfuscation and API obfuscation employed by modern packers, leading to occasional failures during the unpacking process. This paper examines the OEP and API obfuscation techniques utilized by various packers and introduces a system designed to automatically de-obfuscate them. The system analyzes the memory of packed programs, detects trampoline codes, and identifies obfuscated information, for program reconstruction. Experimental results demonstrate the effectiveness of our system in de-obfuscating programs that have undergone OEP and API obfuscation techniques.

Quantitative Visualization of Dissolved Oxygen Concentration Field in Micro Flows using PtOEP/PS Membrane (마이크로 유동에서 PtOEP/PS 박막을 이용한 용존 산소 농도장의 정량적 가시화)

  • Song, Dae-Hun;Kim, Hyun-Dong;Kim, Kyung-Chun
    • Journal of the Korean Society of Visualization
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    • v.9 no.1
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    • pp.36-41
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    • 2011
  • It is highly needed to measure the dissolved oxygen (DO) concentration field in water for a variety of purposes such as biological, industrial, environmental monitoring and medical application. Application of PSP (Pressure Sensitive Paint) which is sensitive to oxygen concentration has been carried out to measure DO concentration field using PtOEP/PS film and intensity based method under the UV-LEDs illumination. A micro round water jet having 100% of DO was obliquely impinged on to a PtOEP/PS film coated plate placed in a 0% of DO water container. DO concentration fields on the impinging plate were quantitatively visualized with a $2.94\;{\mu}m$ of spatial resolution. Through pixel-by-pixel calibration, uncertainty of each pixel by different sensitivity, different dye concentration and non-uniformity of illumination was removed. It is demonstrated that the high DO concentration region was coincided with the impingement area. The DO concentration gradient due to DO diffusion was affected by Reynolds number.

A Study on the distribution of Anti-OEP, protease and elastase HA titer of Pseudomonas aeruginosa in healthy mother and infant (한국 건강유아(健康乳兒) 및 모체(母體)에 있어서 녹농균(綠膿菌)의 OEP, protease 및 elastase에 대(對)한 혈구응집가(血球凝集價))

  • Kim, Hae-Keun;Cho, Yang-Ja
    • The Journal of the Korean Society for Microbiology
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    • v.15 no.1
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    • pp.39-46
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    • 1980
  • This experiment has been made to evaluate the distribution of OEP-HA titer, protease-HA titer and elastase-HA titer of antibody which are the common antigen of Pseudomonas aeruginosa in the serum of healthy mother-newborn infant-pairs, term pregnancy women and under 7 month old infants. By analysing the normal limt of these antibodies, it is expected that the result can be clinically applicable to the diagnosis, management and prevention of Pseudomonas aeruginosa infection, which shows rapid increase nowadays, 1. The Anti OEP-HA titer showed under 1:8, a very low titer, in 73% of cord sera group, but over 1:32 in 68% of mother sera group. 2. In healthy mother-newborn infant-pairs group, the anti OEP-HA titer of mothers showed 1:56, 48, much higher than that of newborn infant, 1:16.42. from which it can be concluded that their titer has no relation between the two. 3. The anti OEP-HA titers of healthy mother and term pregnancy women showed 1:56.5 and 1:53, 4, respectively, which are very similar to each ether, but in infant group, the titer showed 1:33, 51 higher than that of cord sera, 1:16.42 4. The anti protease-HA titer showed under 1:8 in 64-77.5% of total cases, which is lower than that of anti OEP-HA titer. 5. The anti elastase-HA titer showed under 1:8 in 93.75% of cord sera group and in 70.27-76.37% of infant, term perenancy women and mother group, but in infant group, the anti elastase-HA titer showed 1:16.56 higher than that of cord sera group 1:8.5.

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Effect of Supplementation of Trehalose, Glycerol on Conventional Freezing and Vitrification of Boar Sperm

  • Choi, Sun-Ho;Lee, Mi-Jin;Lee, Kyung-Mi;Sa, Soo-Jin;Kim, Hyun-Jong;Jin, Hyun-Ju;Song, Yong-Sup;Park, Jun-Cheol
    • Journal of Embryo Transfer
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    • v.29 no.4
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    • pp.397-401
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    • 2014
  • The boar sperm has more lipid droplets and specialty of seminal plasma compared with other species, causing difficulties of freezing sperm and decreases for the utilization of frozen semen into the artificial insemination. However, several studies reported significant results for the recovery of sperm motility and reproductive by addition of cryoprotectants and seminal plasma after thawing. This study was designed to investigate the effects of supplementation of trehalose or glycerol in the LEY (lactose and egg yolk in BTS) solution for the conventional freezing and vitrification process. Two boars aged 16 months were used to collect semen for 2 times in a week. The samples were allotted to 3 freezing solutions (LEY + glycerol 10.5% + OEP 1.5%, LEY + trehalose 1M + OEP 1.5%, and sucrose 1.5M + trehalose 1 M + OEP 1.5%) after centrifugation at 800 g for 10 minutes. Semen was equilibrated in freezing solutions for 10 minutes and injected into plastic straws with 2~3 air bubbles to minimize freezing damages. Vitrification was performed to locate sperm in 5 cm above $LN_2$ for 5 minutes, and the conventional freezing was conducted with an automatic freezer. Motility and survival rates were measured by CASA (Computer assisted sperm an alyzing system) and FITC (Fluorescein isothiocyanate), respectively after thawing semen at $50^{\circ}C$ for 12 seconds. The results were analyzed by ANOVA with STATVIEW statistical program. The vitrificatioin solution (LEY + 10.5% glycerol + 1.5% OEP) presented higher motility (20.9%) than other solutions while the solution (LEY + 1M trehalose + 1.5% OEP) showed the lowest (motility : 5.2%). However, survival rates of vitrified sperms detected by FITC showed 1~4% live sperms in almost of dead sperms at all vitrification solutions' groups, but survival rate of freezing solution of LEY + 1M trehalose + 1.5% OEP LEY and LEY + 10.5% glycerol + 1.5% OEP were showed 49%, and 79%, respectively. There were differences (P<0.05) survival rate of conventional freezing in LEY + 10.5% glycerol + 1.5% OEP and LEY + 1M trehalose + 1.5% OEP and the remaining showed no differences. The results suggested that vitrified boar semen was not enough to be utilized for the artificial insemination, but it showed possibility to utilize for ICSI and conventional freezing with glycerol would be useful method for artificial insemination in pig while we choose the outstanding semen against tolerance to freezing damages.

Optical study on the morphology of organic molecules in thin solid films

  • Tsuboi, Taiju
    • 한국정보디스플레이학회:학술대회논문집
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    • 2006.08a
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    • pp.577-582
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    • 2006
  • Unlike the case of $Ir(ppy)_3$, various aggregates of PtOEP including dimer are formed in PtOEP-doped films and neat film. Such a difference is due to difference of competition among solid state solvation effect, dipole-dipole interactions between dopant molecules, and intermolecular covalent bonding.

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Effect of Host Materials on Eelectrophosphorescence Properties of PtOEP-doped Organic Light-emitting Diodes

  • Kang, Gi-Wook;Lee, Chang-Hee
    • Journal of Information Display
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    • v.8 no.2
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    • pp.15-19
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    • 2007
  • We have studied the effect of host materials on the electrophosphorescence properties by comparing three different host materials such as tris(8-hydroxyquinoline)-aluminum (III) $(Alq_3)$, bis(8-hydroxyquinoline)-zinc (II) $(Znq_2)$, and 4,4'-N,N' dicarbazole-biphenyl (CBP) doped with a red-emissive phosphorescent dye, 2,3,7,8,12,13,17,18-octaethyl-21H,23H-porphyrin platinum (II) (PtOEP). The EL spectra show a strong red emission (peak at 650 nm) from the triplet excited state of PtOEP and a very weak emission from an electron transport layer of $Alq_3$ and a hole transport layer of N,N'-diphenyl-N,N'-bis(3-methylphenyl)-1,1-biphenyl-4,4'-diamine (TPD). We find that the triplet exciton lifetime and the quantum efficiency decrease in the order of CBP, $Alq_3$, and $Znq_2$ host materials. The results are interpreted as a poor exciton confinement in $Alq_3$, and $Znq_2$ host compared with in CBP. Therefore, it is very important for the triplet-exciton confinement in the emissive layer for obtaining a high efficiency.