• Korean Journal of Fisheries and Aquatic Sciences
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• v.57 no.3
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• pp.239-252
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• 2024

Recombination activating genes (RAGs) play a crucial role in initiating V(D)J recombination, which is essential for developing adaptive immunity in vertebrates. In this study, we cloned and characterized RAG1/2 cDNA from the marine medaka Oryzias dancena (OdRAG1/2) and investigated their mRNA expression patterns during ontogenetic developmental stages. The OdRAG1 and OdRAG2 cDNA contained open reading frames (ORFs) encoding proteins containing 1,078 and 531 amino acids, respectively. Multiple sequence alignment and phylogenetic analysis revealed that OdRAG1 and OdRAG2 are highly conserved with their corresponding orthologs, featuring distinct core and non-core regions. Notably, expression analysis showed that, in contrast to other fish RAGs studied, OdRAG1/2 expression peaked at 0 days post-hatching (DPH). Additionally, for the expression of T and B cell differentiation markers, CD3γ and CD20, also peaked at 0 DPH. Collectively, adaptive immunity in O. dancena potentially begins during embryonic development, which is critical for V(D)J recombination and essential immune component development, suggesting the early ontogenetic stage interactions between innate and adaptive immunity.

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.141-148
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• 2017

The oxidation ditch (OD) is one of the most widely used processes for treating municipal wastewater. However, the microbial communities in the OD systems have not been well characterized, and little information about the shift of bacterial community during the startup process of the OD systems is available. In this study, we investigated the bacterial community changes during the startup period (over 100 days) of a full-scale OD. The results showed that the bacterial community dramatically changed during the startup period. Similar to the activated sludge samples in other studies, Proteobacteria (accounting for 26.3%-48.4%) was the most dominant bacterial phylum in the OD system, but its relative abundance declined nearly 40% during the startup process. It was also found that Planctomycetes proliferated greatly (from 4.79% to 13.5%) and finally replaced Bacteroidetes as the second abundant phylum in the OD system. Specifically, some bacteria affiliated with genus Flavobacterium exhibited remarkable decreasing trends, whereas bacterial species belonging to the OD1 candidate division and Saprospiraceae family were found to increase during the startup process. Despite of the bacterial community shift, the organic matter, nitrogen, and phosphorus in the effluent were always in low concentrations, suggesting the functional redundancy of the bacterial community. Moreover, by comparing with the bacterial community in other municipal wastewater treatment bioreactors, some potentially novel bacterial species were found to be present in the OD system. Collectively, this study improved our understandings of the bacterial community structure and microbial ecology during the startup of a full-scale wastewater treatment bioreactor.

• Microbiology and Biotechnology Letters
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• v.45 no.2
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• pp.149-154
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• 2017

To increase the efficiency of esterase production by Bacillus, high cell-density culture of recombinant Escherichia coli through fed batch fermentation was tested. Cells were cultured to $OD_{600}$ of 76 (35.8 g/l DCW) with dissolved oxygen level controlled to least above 30% air saturation by supplying pure oxygen. Cells were cultured to an $OD_{600}$ of 90 (42.4 g/l DCW) with glucose feeding controlled to at least 1 g/l. However, the cells reached stationary phase at the late stage of culture, despite glucose being supplied. Cells were cultured to an $OD_{600}$ of 185 (87.3 g/l DCW) by supplying additional medium with fortified yeast extract. To increase the productivity of the recombinant protein, cell growth and esterase productivity based on induction time were evaluated. Late exponential phase induction for esterase production in fed batch fermentation resulted in maximum optical density $OD_{600}$ of 190 (89 g/l DCW) and maximum esterase activity of 1745 U/l, corresponding to a 5.8-fold enhancement in esterase production, compared to the early exponential phase induction. In this study, we established fermentation methods for achieving maximum production of Bacillus-derived esterase by optimizing IPTG induction time in high-cell density culture by supplying pure oxygen and a nitrogen source.

• Journal of Astronomy and Space Sciences
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• v.34 no.4
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• pp.271-280
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• 2017

This study presents the application of satellite laser ranging (SLR) to orbit determination (OD) of high-Earth-orbit (HEO) satellites. Two HEO satellites are considered: the Quasi-Zenith Satellite-1 (QZS-1), a Japanese elliptical-inclinedgeosynchronous-orbit (EIGSO) satellite, and the Compass-G1, a Chinese geostationary-orbit (GEO) satellite. One week of normal point (NP) data were collected for each satellite to perform the OD based on the batch least-square process. Five SLR tracking stations successfully obtained 374 NPs for QZS-1 in eight days, whereas only two ground tracking stations could track Compass-G1, yielding 68 NPs in ten days. Two types of station bias estimation and a station data weighting strategy were utilized for the OD of QZS-1. The post-fit root-mean-square (RMS) residuals of the two week-long arcs were 11.98 cm and 10.77 cm when estimating the biases once in an arc (MBIAS). These residuals were decreased significantly to 2.40 cm and 3.60 cm by estimating the biases every pass (PBIAS). Then, the resultant OD precision was evaluated by the orbit overlap method, yielding three-dimensional errors of 55.013 m with MBIAS and 1.962 m with PBIAS for the overlap period of six days. For the OD of Compass-G1, no station weighting strategy was applied, and only MBIAS was utilized due to the lack of NPs. The post-fit RMS residuals of OD were 8.81 cm and 12.00 cm with 49 NPs and 47 NPs, respectively, and the corresponding threedimensional orbit overlap error for four days was 160.564 m. These results indicate that the amount of SLR tracking data is critical for obtaining precise OD of HEO satellites using SLR because additional parameters, such as station bias, are available for estimation with sufficient tracking data. Furthermore, the stand-alone SLR-based orbit solution is consistently attainable for HEO satellites if a target satellite is continuously trackable for a specific period.

• Parasites, Hosts and Diseases
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• v.23 no.1
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• pp.95-101
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• 1985

Fascioliasis in cattle is one of the most common and very serious trematode diseases in Korea. In the present study, the enzyme linked immunosorbent assay (ELISA) was applied in the diagnosis of fascioliasis using antigen of Fasciela hepatica, perokidase of conjugate anti-cattle Is G and orthophenylenediamine as a substrate by micro-method technique of Volley et at. (1976b) and MacLaren (1978) with a slight modification. Results obtained from the present study are as follows. 1. In assay for optimal dilution of stock antigen, the antigen (protein contents; 0. Bmgymz) was diluted from 1150 to 1/600 with carbonate buffer (pH 9.6), and then absorbance values were measured with 1/100 diluted sera. The regression equations between the OD values of ELISA and dilution of antigen were log Y: -0.181-0.00127X in infected sera, and log Y: -0.578-0. 000879X in normal sera. The significantly higher (p<0.05) OD value was observed in the former. 2. In assay for optimal dilution of sera, the sera were diluted from 1125 to 1/400 with in PBSJ Tween 20 (pH 7.4), and absorbance values were measured with 1/200 diluted antigen. The regression equation between the OD values of ELISA and dilution of sera were log Y: -0.1540-0.0007238X in infected sera and log Y: -0.4834-0.00116X in normal sera. The former was higher than the latter (p<0.05). 3. In the 27 cases of negative intradermal test, OD values of the ELISA are $0.447{\pm}0.144$, the 95% confidence interval (Mean+2 H SD) of the values was 0.735, and there was no case over the values. Therefore, the sensitivity of the antigen to diagnose fascioliasis was 100% in the negative case. The OD value 0.7 which is designed as a criterion (detection level of positive one) is useful for the performance of the ELISA in fascioliasis. 4. According to the OD value of criterion in the regression equations, the optimal dilutions of stock antigen and serum were 1/250 and 1/100, respectively. 5. In the 58 cases of fascioliasis from which the adult could be found in the bile ducts, the OD value was $0.846{\pm}0.224$. The 75% (44 cattle) among them had higher value with compared to the criterion, and the 60% (20 cattle) of the cases of proliferative cholangitis of 33 cattle which had been infected previousely with Fasciola sp. is higher than the criterion. 6. Prevalence of fascioliasis was 43.4% in the application of the ELISA to 272 cattle which were reared in Jeonbug district.

• Korean Journal of Food Science and Technology
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• v.40 no.2
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• pp.178-183
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• 2008

This study investigated the effects of osmotic dehydration (OD) and vacuum impregnation (VI) on the quality of dried apple products. Weight reduction and water loss increased during OD, but these decreased in the apples during VI. In particular, VI's effect on increasing solid gain was superb. For apples in 40% sucrose solution, OD and VI were followed by hot-air drying at 50$^{\circ}C$. The experimental data were fitted successfully using the modified Page model. OD and VI increased drying time and decreased the drying rate constant of these apples as compared to the control. Shrinkage and rehydration capacity greatly decreased in the apples dried by OD and increased in the apples dried by VI as compared to the control. OD also decreased titratable acidity and ascorbic acid content considerably. Sensory evaluations of the products indicated that the apples prepared by OD had higher palatability in their rehydrated form in yoghurt, and the apple products prepared by VI had higher palatability in their dried form.

• Journal of the Korean Mathematical Society
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• v.45 no.4
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• pp.1075-1087
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• 2008

We study a class of KMS-symmetric quantum Markovian semigroups on a quantum spin system ($\mathcal{A},{\tau},{\omega}$), where $\mathcal{A}$ is a quasi-local algebra, $\tau$ is a strongly continuous one parameter group of *-automorphisms of $\mathcal{A}$ and $\omega$ is a Gibbs state on $\mathcal{A}$. The semigroups can be considered as the extension of semi groups on the nontrivial abelian subalgebra. Let $\mathcal{H}$ be a Hilbert space corresponding to the GNS representation con structed from $\omega$. Using the general construction method of Dirichlet form developed in [8], we construct the symmetric Markovian semigroup $\{T_t\}{_t_\geq_0}$ on $\mathcal{H}$. The semigroup $\{T_t\}{_t_\geq_0}$ acts separately on two subspaces $\mathcal{H}_d$ and $\mathcal{H}_{od}$ of $\mathcal{H}$, where $\mathcal{H}_d$ is the diagonal subspace and $\mathcal{H}_{od}$ is the off-diagonal subspace, $\mathcal{H}=\mathcal{H}_d\;{\bigoplus}\;\mathcal{H}_{od}$. The restriction of the semigroup $\{T_t\}{_t_\geq_0}$ on $\mathcal{H}_d$ is Glauber dynamics, and for any ${\eta}{\in}\mathcal{H}_{od}$, $T_t{\eta}$, decays to zero exponentially fast as t approaches to the infinity.

• Journal of Broadcast Engineering
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• v.8 no.3
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• pp.259-267
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• 2003

In this paper, we propose a media processor to provide interactive services in mobile multimedia broadcasting environments. The proposed system Is designed to support various functionalities, such as generation of MPEG-4 IOD (Initial Object Descriptor)/OD(Object Descriptor)/BIFS (Binary Format for Scene) data, encapsulation of MPEG-4 AVC (Advanced Video Coding)/BSAC (Bit Sliced Arithmetic Coding) stream and generated IOD/OD/BIFS data into SL (Sync Layer) packet, packetization of SL packet into TS (Transport Stream) packet and multiplexing. The proposed media processor can provide MPEG-4 based interactive services for users.

• Journal of Korean Society of Rural Planning
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• v.23 no.2
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• pp.43-54
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• 2017

When an age of low growth and population decline, population migration plays an important role in spatial structure of region. There have been many researches on migration and regional spatial structure. The purpose of this study is to examine the changes of Gwangju and Jeonnam region's spatial structure and central area using social network analysis methods. For analysis it was used that population and migration data and passenger OD(Origin and Destination) travel data released by Statistics Korea and Korea Transport Database(KTDB). Using Gephi 0.8.2, migration and passenger OD networks were visualized, and this describe network flow and density. The results of the network centrality analysis show that the most populated village is not always network center though population mass is an important factor of central places. The average eigenvector centrality of 2010 migration is the lowest during 2005-2015, and it means few regions have high centralities. When comparing migration and travel networks, travel data is more effective than migration data in determining the central location considering spatial functions.

• Proceedings of the KSR Conference
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• 2010.06a
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• pp.1148-1158
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• 2010

This paper addresses prospect of the technology for development of NxTIS (NeXt generation Transport Information System) that is targeted to shifting Korean public transit framework to railway-centered one. The Seoul metropolitan area has adopted the smart-card as a payment system for the public transportation since 2004. The transportation record can be used as quite useful information for better planning and operating of the railway system, particularly when the smart-card based payment system is extended to whole nation. This paper introduces technologies to develop Real OD DB and NxTIS, and presents road-map for them. A variety of benefits from the development of the system are estimated both in quantitative and qualitative. The Real OD DB based NxTIS will work to highly prospective for creating a novel value in the railway as a public transportation system.