• Korean Journal of Plant Resources
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• v.11 no.2
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• pp.136-141
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• 1998

To give some information on the harvest and storage of Chinese yam (Dioscorea opposita), the experiment was done to examine the effect of its harvesting date and storage method on tuber weight, tuberrelated characters during the storageor at the end of storage. The yam tubers harvested 4 times at 20 days interval from Oct. 15 were stored with different storage methods(pit, room and cold temperature) and on Mar.15. all the characters were measured. Fresh weight of the tuber harvestedafter Nov. 5 and stored were greater than that done on Oct. 15 while dry tuber weight did not have any difference between the harvesting dates. The fresh weigth done directly from the field on Mar. 15 was higher compared to the other harvesting dates owing to its higher moisture content. The rate of sound tuber stored at room or cold temperature condition was higher than that at the field at which the rate was greater at the harvest after late nov. compared to the earlier harvests. Although the two storages were similar at sound tuber rates and marketable yield, the tuber weight was more reduced when stored at room temperature than when done at cold temperature . Brightness and value 'b' of chromaticaity realted to the tuber coloring and sugar content were higher but moisture content was lowerwhen harvested on Oct. 15 than the other havesting dates. All the characteristics realted to the marketability after storage were nearly same when stored at room and cold temperature. Value 'a' of chromaticity and sugar content were higher when harvest on Mar. 15 than when harvested before Dec. 15 but the 'b' value and mositure content were reverse results.

• Journal of Genetic Medicine
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• v.12 no.1
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• pp.31-37
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• 2015

Purpose: We investigated the neurogenic potentials of amniotic fluid-derived stem cells (AFSCs) according to the expression levels of stem cell markers and ingredients in the neural induction media. Materials and Methods: Four samples of AFSCs with different levels of Oct-4 and c-kit expression were differentiated neurally, using three kinds of induction media containing retinoic acid (RA) and/or a mixture of 3-isobutyl-1-methylxanthine/indomethacin/insulin (neuromix), and examined by immunofluorescence and reverse transcription-polymerase chain reaction (RT-PCR) for their expression of neurospecific markers. Results: The cells in neuromix-containing media displayed small nuclei and long processes that were characteristic of neural cells. RT-PCR analysis revealed that the number of neural markers showing upregulation was greater in cells cultured in the neuromix-containing media than in those cultured in RA-only medium. Neurospecific gene expression was also higher in Oct-4 and c-kit double-positive cells than in c-kit-low or -negative cells. Conclusion: The stem cell marker c-kit (rather than Oct-4) and the ingredient neuromix (rather than RA) exert greater effects on neurogenesis of AFSCs.

• Journal of the Optical Society of Korea
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• v.18 no.1
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• pp.55-59
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• 2014

Sarcopenia, or reduced muscle mass and volume, is due to various factors such as senile change, neuronal degeneration, drug, malignancy, and sepsis. Sarcopenia with the aging process has been evidenced by the decline in muscle mass by 0.5 to 1% per year with 3-5% reduction in muscle strength for 10 years between the ages of 40 and 50, and a 1-2% of decline of mass every year in people aged 60-70. Therefore, early diagnosis and understanding the mechanism of sarcopenia are crucial in the prevention of muscle loss. However, it is still difficult to image changes of muscle microstructure due to a lack of techniques. In this study, we developed an animal model using denervated rats to induce a rapid atrophy in the tibialis anterior (TA) and imaged its structural changes using optical coherence tomography (OCT) along with histologic and ultrasound analyses. Ultrasound showed changes of overall muscle size. Histology revealed that the atrophic TA muscle displayed an increased size variability of muscle fiber and inflammatory changes. Three dimensional OCT imaged the changes of perimysial grid and muscle fiber structure in real time without sacrifice. These observed advantages of multimodal imaging using OCT and ultrasound would provide clinical benefits in the diagnosis of sarcopenia.

• International Journal of Highway Engineering
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• v.15 no.6
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• pp.93-101
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• 2013

PURPOSES : The rumble strip installed at the highway near the tollgate has the purpose to reduce the vehicle velocity or prevent sleepiness by awakening people to the danger. These rumble strip has different vibration decibel from the rumble strip shapes, resulting in some fatigue damage to human because a driver suffers from a lot of stress and displeasure. In this connection, the objective of this paper is to analyze the vibration decibel perceived by a driver in the vehicle under some conditions. METHODS : The vibration decibel conveyed from the tire can be analyzed. The frequency analysis methods were used according to DFT (Discrete Fourier Transform) analysis, FFT (Fast Fourier Transform) analysis, CPB (Constant Percentage Bandwidth) analysis. But the frequency analysis method in this paper is the 1/24 OCT(Octave) band analysis because of the convenience of the analysis and the overall vibration amplitude along the frequency. RESULTS : By using the results of the vibration decibel after analyzing the 1/24 OCT band analysis, these results can be compared from some conditions (e.g., rumble strip shape, uniform velocity of a vehicle, road condition, mass of a vehicle). As a result, the numerical values of decibel are not directly proportional to the vehicle velocity. CONCLUSIONS : At the condition that a vehicle is passing by the rumble strip, the value of a vibration decibel at the rumble strip of the cylinder shape is smaller than the rumble strip of rectangular shape regardless of the rumble strip depth and width. At the mass condition, the more a vehicle is massive, the more the vibration decibel increases. At the road condition, the vibration decibel at the wet road is smaller than the value at dry road condition.

• Korean Journal of Ophthalmology
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• v.32 no.6
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• pp.470-477
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• 2018

Purpose: To investigate the relationship between the progression of visual field (VF) loss and changes in lamina cribrosa depth (LCD) as determined by spectral-domain optical coherence tomography (SD-OCT) enhanced depth imaging in patients with primary open angle glaucoma (POAG). Methods: Data from 60 POAG patients (mean follow-up, $3.5{\pm}0.7$ years) were included in this retrospective study. The LCD was measured in the optic disc image using SD-OCT enhanced depth imaging scanning at each visit. Change in the LCD was considered to either 'increase' or 'decrease' when the differences between baseline and the latest two consecutive follow-up visits were greater than the corresponding reproducibility coefficient value ($23.08{\mu}m$, as determined in a preliminary reproducibility study). All participants were divided into three groups: increased LCD (ILCD), decreased LCD (DLCD), and no LCD change (NLCD). The Early Manifest Glaucoma Trial criteria were used to define VF deterioration. Kaplan-Meier survival analysis and Cox's proportional hazard models were performed to explore the relationship between VF progression and LCD change. Results: Of the 60 eyes examined, 35.0% (21 eyes), 28.3% (17 eyes), and 36.7% (22 eyes) were classified as the ILCD, DLCD, and NLCD groups, respectively. Kaplan-Meier survival analysis showed a greater cumulative probability of VF progression in the ILCD group than in the NLCD (p < 0.001) or DLCD groups (p = 0.018). Increased LCD was identified as the only risk factor for VF progression in the Cox proportional hazard models (hazard ratio, 1.008; 95% confidence interval, 1.000 to 1.015; p = 0.047). Conclusions: Increased LCD was associated with a greater possibility of VF progression. The quantitative measurement of LCD changes, determined by SD-OCT, is a potential biomarker for the prediction of VF deterioration in patients with POAG.

• Journal of Practical Agriculture & Fisheries Research
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• v.14 no.1
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• pp.47-59
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• 2012

The effects of organic calcium compounds (ACa) tree-spray for late growing stage on the mineral nutrition concentration of fruit, russet and quality in 'Fuji' apple were investigated. Optimum spray concentration of leaves by ACa tree-spray during late growth stage were ranged 125 times among 1,000 times. Also, Optimum concentration of fruits was effected to 125 times. Optimum spray times of leaves effected by ACa 500 times tree-spray during late growth stage were effective in one times (Sept. 25) and three times (Sept. 25, Oct. 5, 15). Also, Optimum times concentration of fruit skin was effected to one times (Sept. 25) and fruit flesh was effected two times (Sept. 25, Oct. 5) and three times (Sept. 25, Oct. 5, 15). Phytotoxicity by tree-spray of ACa concentration and times different not occur to leaf and fruit.

• Journal of Animal Reproduction and Biotechnology
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• v.38 no.4
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• pp.199-208
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• 2023

Background: Canine induced pluripotent stem cells (iPSCs) are an attractive source for veterinary regenerative medicine, disease modeling, and drug development. Here we used vitamin C (Vc) to improve the reprogramming efficiency of canine iPSCs, and its functions in the reprogramming process were elucidated. Methods: Retroviral transduction of Oct4, Sox2, Klf4, c-Myc (OSKM), and GFP was employed to induce reprogramming in canine fetal fibroblasts. Following transduction, the culture medium was subsequently replaced with ESC medium containing Vc to determine the effect on reprogramming activity. Results: The number of AP-positive iPSC colonies dramatically increased in culture conditions supplemented with Vc. Vc enhanced the efficacy of retrovirus transduction, which appears to be correlated with enhanced cell proliferation capacity. To confirm the characteristics of the Vc-treated iPSCs, the cells were cultured to passage 5, and pluripotency markers including Oct4, Sox2, Nanog, and Tra-1-60 were observed by immunocytochemistry. The expression of endogenous pluripotent genes (Oct4, Nanog, Rex1, and telomerase) were also verified by PCR. The complete silencing of exogenously transduced human OSKM factors was observed exclusively in canine iPSCs treated with Vc. Canine iPSCs treated with Vc are capable of forming embryoid bodies in vitro and have spontaneously differentiated into three germ layers. Conclusions: Our findings emphasize a straightforward method for enhancing the efficiency of canine iPSC generation and provide insight into the Vc effect on the reprogramming process.

• Journal of Space Technology and Applications
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• v.4 no.1
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• pp.74-85
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• 2024

As the limitations of conventional radio communications between satellites and the ground become apparent, various experiments are being conducted around the world to overcome them with space laser communication. In this study, we address the development of our own optical communications terminal (OCT) and optical ground station (OGS) and the experiments of free-space optical communication (FSOC) using them. Using a 30 mm-diameter OCT and a 250 mm-diameter portable OGS telescope, as well as commercial 10 Gbps SFP+ modules and media converters, we successfully transmitted and received 4K high-definition multimedia interface (HDMI) signals through 1,550 nm optical laser beam. The transmission and reception distances of the experiment were 3, 9, and 20 km, respectively, and the received signal strength at each distance was +6.1, -2.8, and -10.9 dBm, respectively. It was demonstrated that the 4K HDMI video lasted for over 10 minutes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.6
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• pp.400-404
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• 2000

Hairy vetch (vicia villosa Roth), leguminous green manure crop, can increase soil fertility and reduce chemical nitrogen application for cash crop such as spring corn. More yield of hairy vetch is needed at planting cash crop to obtain higher effect of green manure. Hairy vetch waa seeded on Sep.10, Oct.1, and Oct. 20 and at 10, 20, 30 and 40 kg/ha of seeding rate respectively, in 1996 and 1997, Dry matter and nitrogen yield of hairy vetch were measured on May 1 in 1997 and 1998. Proper seeding rate of hairy vetch was 30 kg/ha irrespective of years and seeding dates. Above-ground dry matters of hairy vetch on May 1 in 1997 and 1998 decreased according to delayed seeding, and those were 5.5-7, 4-4.5, 1.3-2.2 ton/ha on Sep. 10, Oct. 1 and Oct 20 of seeding date, respectively in seeding rate 30-40 kg/ha. Also nitrogen yield of hairy vetch on May 1 decreased according to delayed seeding, and those were 220-280, 160-180, 60-100kg/ha on Sep. 10, Oct. 1 and Oct 20 of seeding date, respectively in seeding rate 30-40kg/ha. Therefore, we suggest that hairy vetch has to be seeded earlier in autumn to obtain high green manure yield in spring. To determine the detailed optimum seeding time in autumn, hairy vetch was seeded on Aug. 20, Aug. 31, Sep. 10, Sep. 20, and Sep. 30 in 1999 and was harvested on April 22, April 27, and May 2 in 2000, respectively. Dry matter and nitrogen yield of hairy vetch by seeding in late August were higher than those by seeding in September indicating that dry matter of hairy vetch were 7-8, 6-7, 4-5, 2-3 ton/ha and nitrogen yield were 240-290, 200-260, 150-220, 70-120 kg/ha, respectively when seeded on Aug. 20, Aug. 31, Sep. 10, Sep. 20 and Sep. 30 and harvested on April 22-May 2. Increase of dry matter and N yield of hairy vetch by 10days delayed harvest was higher in late August seeding than in September seeding. So hairy vetch should be seeded in late August if possible to obtain much more green manure yield and be seeded until September because green manure yield decrease rapidly when seeded after October.

• Development and Reproduction
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• v.14 no.4
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• pp.243-251
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• 2010

ESRRB (Estrogen related receptor $\beta$) is an orphan receptor, and have a role on maintaining the undifferentiated state and self-renewal of pluripotent stem cell as a transcription factor which regulates the expression of OCT4 and NANOG genes. Also, Feng et al. (2009) reported that Esrrb, Oct4 and Sox2 could induce pluripotent stem cell from somatic cells. The aim of the present study was to develop the direct delivery system of human ESRRB protein into human amniotic fluid-derived stem cells (AFSCs) and to analyze the effect of ESRRB on the regulation of pluripotency-related genes. Human ESRRB has three isoforms arisen by alternative splicing. We cloned short-form ESRRB and made a fusion protein of ESRRB and R7 for an efficient protein transfer to cell. R7 as cell-penetrating peptide(CPP) can help to transfer ESRRB into cells. R7-ESRRB-His6 protein was observed in the cytoplasm and nuclei within 5 hours after treatment. Also, we could observe R7-ESRRB-His6 protein only in the nuclei within 24 hours. Realtime PCR showed that ESRRB increased expression of OCT4 and NANOG as well as SOX2 gene. Therefore, we demonstrated that R7-ESRRB-His6 proteins were efficiently transferred into the nuclei of AFSCs and work well as a possible transcription factor.