Journal of Genetic Medicine

Korean Society of Medical Genetics and Genomics (대한의학유전학회)
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Neurogenic potentials of human amniotic fluid-derived stem cells according to expression levels of stem cell markers and ingredients of induction medium

  • Lim, Eun Hye (Adult Stem Cells Research Institute, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine) ;
  • Cho, Jung Ah (Adult Stem Cells Research Institute, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine) ;
  • Park, Ho (Adult Stem Cells Research Institute, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine) ;
  • Song, Tae Jong (Department of Obstetrics and Gynecology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine) ;
  • Kim, Woo Young (Department of Obstetrics and Gynecology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine) ;
  • Kim, Kye Hyun (Department of Obstetrics and Gynecology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine) ;
  • Lee, Kyo Won (Adult Stem Cells Research Institute, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine)
  • Received : 2015.04.10
  • Accepted : 2015.06.17
  • Published : 2015.06.30
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Abstract

Purpose: We investigated the neurogenic potentials of amniotic fluid-derived stem cells (AFSCs) according to the expression levels of stem cell markers and ingredients in the neural induction media. Materials and Methods: Four samples of AFSCs with different levels of Oct-4 and c-kit expression were differentiated neurally, using three kinds of induction media containing retinoic acid (RA) and/or a mixture of 3-isobutyl-1-methylxanthine/indomethacin/insulin (neuromix), and examined by immunofluorescence and reverse transcription-polymerase chain reaction (RT-PCR) for their expression of neurospecific markers. Results: The cells in neuromix-containing media displayed small nuclei and long processes that were characteristic of neural cells. RT-PCR analysis revealed that the number of neural markers showing upregulation was greater in cells cultured in the neuromix-containing media than in those cultured in RA-only medium. Neurospecific gene expression was also higher in Oct-4 and c-kit double-positive cells than in c-kit-low or -negative cells. Conclusion: The stem cell marker c-kit (rather than Oct-4) and the ingredient neuromix (rather than RA) exert greater effects on neurogenesis of AFSCs.

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References

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