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http://dx.doi.org/10.5734/JGM.2015.12.1.31

Neurogenic potentials of human amniotic fluid-derived stem cells according to expression levels of stem cell markers and ingredients of induction medium  

Lim, Eun Hye (Adult Stem Cells Research Institute, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine)
Cho, Jung Ah (Adult Stem Cells Research Institute, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine)
Park, Ho (Adult Stem Cells Research Institute, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine)
Song, Tae Jong (Department of Obstetrics and Gynecology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine)
Kim, Woo Young (Department of Obstetrics and Gynecology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine)
Kim, Kye Hyun (Department of Obstetrics and Gynecology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine)
Lee, Kyo Won (Adult Stem Cells Research Institute, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine)
Publication Information
Journal of Genetic Medicine / v.12, no.1, 2015 , pp. 31-37 More about this Journal
Abstract
Purpose: We investigated the neurogenic potentials of amniotic fluid-derived stem cells (AFSCs) according to the expression levels of stem cell markers and ingredients in the neural induction media. Materials and Methods: Four samples of AFSCs with different levels of Oct-4 and c-kit expression were differentiated neurally, using three kinds of induction media containing retinoic acid (RA) and/or a mixture of 3-isobutyl-1-methylxanthine/indomethacin/insulin (neuromix), and examined by immunofluorescence and reverse transcription-polymerase chain reaction (RT-PCR) for their expression of neurospecific markers. Results: The cells in neuromix-containing media displayed small nuclei and long processes that were characteristic of neural cells. RT-PCR analysis revealed that the number of neural markers showing upregulation was greater in cells cultured in the neuromix-containing media than in those cultured in RA-only medium. Neurospecific gene expression was also higher in Oct-4 and c-kit double-positive cells than in c-kit-low or -negative cells. Conclusion: The stem cell marker c-kit (rather than Oct-4) and the ingredient neuromix (rather than RA) exert greater effects on neurogenesis of AFSCs.
Keywords
Stem cells; Amniotic fluid; Neurogenesis;
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